31 results about "Gentiana straminea" patented technology

The invention relates to a novel secoiridoid compound and application in the preparation of anti-inflammatory drugs. A novel compound is extracted and separated from gentiana straminea, has a chemical structural general formula shown in a formula I and has anti-inflammatory activity. The invention also relates to a use of the compound or pharmaceutically acceptable salts of the compound in the preparation of drugs, in particular to a use in the preparation of anti-inflammatory drugs. The invention also relates to a pharmaceutical composition taking the compound as a main component.

The present invention relates to a method for preparing gentiopicroside chemical reference substance from Gentiana gentipicroside, the method comprising the following steps: (1) extraction with 95% ethanol solution: drying the whole herb of gentipicroside in the shade, crushing, carrying out alcohol extraction, filtering, and drying to paste , to obtain the alcoholic extract of Gentiana japonica; (2) Resin column enrichment: dissolving the alcoholic extract of Gentiana kinkanii with methanol solution and separating it through a resin column, first eluting with aqueous solution to remove large polar impurities, and then using 30% methanol solution, Step gradient elution with 50% methanol solution, collect 50% of the eluate, and dry the 50% of the eluate under reduced pressure to obtain the crude product of Gentiana japonica; (3) Refined by reverse-phase liquid phase preparative chromatography: after the crude product of Gentiana genus twisted is dissolved in methanol solution , after filtration, reversed-phase liquid phase preparative chromatographic separation, the main chromatographic peak fraction in the preparative chromatogram was collected, and the chromatographic peak fraction was dried under reduced pressure to obtain a white powdery reference substance with a purity greater than 98%. The invention has short separation time, good separation effect and high product purity.

The invention relates to a process for extracting gentiopicrin from flowers of gentiana straminea. The process is as follows: after the dried flower of gentiana straminea is crushed, cold maceration and extraction is carried out at room temperature taking alcohol as an extraction solvent, and gentiopicrin with higher purity is obtained through concentration, absorption and drying. The invention makes a study of the extraction and preparation process of gentiopicrin in gentiana straminea for the first time, determines the optimal cold maceration and extraction conditions and the purification process of the gentiopicrin in the gentiana straminea, and meets the requirements of developing various medicines taking gentiana straminea as main raw materials.

The invention relates to a cultivation method of plateau plants, in particular to a bionic cultivation method of gentiana straminea maxim. The method comprises the following steps of: (1) selecting a gentiana straminea maxim planting base; (2) selecting land and treating soil; (3) preparing seeds; (4) planting; (5) building a meshy fence around the planting base when planting the gentiana straminea maxim in pastoral regions at a higher altitude; suppressing weeds as well as preventing and controlling rodents; and overwintering; and (6), harvesting and primarily processing medicinal herbs. Compared with the traditional gentiana planting method, the bionic cultivation method of the gentiana straminea maxim has the advantages that: 1. the incidence rate of annual weeds in the soil with the depth of less than 10cm can be reduced without ploughing (non-tillage) and stirring the soil when planting, thereby saving the cost; 2. the weeding control is carried out before planting by utilizing biopesticide herbicides with short residue longevity so as to suppress perennial weeds, the gentiana is easy to germinate under the overshading and water-retention of withered and yellow weeds, and thegermination speed of the germinate is faster than that of the conventional method at cold areas; and 3. the mechanical cutting is adopted for weeding without pollution, thereby saving the weeding cost.

The invention provides an alpine gentian herb HPLC detection method including the following operation steps: taking a to-be-detected medicinal material standard, and extracting with methanol, and preparing a test sample solution; injecting the test sample solution into a high performance liquid chromatograph, detecting, and building a standard fingerprint atlas; taking a to-be-detected medicinal material, obtaining a fingerprint atlas according to the method with the steps, and comparing the fingerprint atlas with the standard fingerprint atlas. The detection method has good degree of separation, has more peak appearing number, can distinguish multiple varieties of alpine gentian herb from confusion or counterfeit species, and provides a guarantee for the quality of the alpine gentian herb medicinal material.