143 results about "Total antioxidant" patented technology

The present invention provides a performance assay that measures the total antioxidant activity of a composition using oxygen uptake in contrast to prior art methods that measure antioxidant capacity by indirectly measuring degradation of a fluorescent compound by following the disappearance of fluorescence. Using the performance antioxidant assay of the present invention, an antioxidant composition having synergistic activity is provided by the present inventors that includes flavonoids such as the flavonol quercetin, mixed tocopherols or tocotrienols, grape skin extract, green tea extract and bush plum. The antioxidant activity of the present composition exceeds 6,000 micromoles Trolox equivalent units per gram using the present invention.

A composition of cold pressed botanic oils exhibiting antioxidant activity. The composition is a combination of at least two oils, which can be black cumin oil, black raspberry oil, red raspberry oil, pomegranate oil, pumpkin oil, or sesame, flax, chardonnay grape seed oil or any other fruit, herb, vegetable, grain or nut oil. The composition exhibits total antioxidant activity greater than the sum of the weighted average of the antioxidant activities of the individual oils. A method of producing a composition of botanic oils exhibiting antioxidant activity, comprising extracting oil in an oxygen deprived environment from fruits and vegetables and combining the extracted oils. The composition exhibits total antioxidant activity greater than the sum of the weighted average of the antioxidant activities of the individual oils.

The invention relates to a quality assurance and identification method for high-quality strawberries, belonging to the technical field of biology. The quality assurance and identification method comprises the following steps: determining phenols and flavor substances of different varieties of strawberries by virtue of HPLC-MS and GC-MS, and establishing a chemical fingerprint chromatography database; determining the total antioxidant activity of the various strawberries by virtue of an ORAC method; analyzing blastogenesis diversity of the strawberries by utilizing RFLP, RAPD and SSR methods and combining key enzyme genes in synthetic routes of anthocyanin and polyphenol; establishing a spectrum-effect relationship between chemical fingerprint chromatography and biological activity information of the different varieties of strawberries, and analyzing the relation between hereditary basis and functional quality character of the strawberries. The high-quality variety of the strawberries is selected by combining chemical fingerprint chromatography, the effectiveness evaluation, genetic diversity analysis and the spectrum-effect relationship and is subjected to authenticity identification, so that a scientific, reliable, uniform and standard strawberry quality evaluation system is established, and technical support and scientific basis are provided for the quality control of the strawberries.

A method for increasing the longevity of an old animal comprising administering to the animal a composition comprising one or more antioxidants in a total antioxidant amount sufficient to increase the longevity of the animal.

An ophthalmic composition for artificial tears useful for treating dry eye symptoms or for general eye protection. The composition includes a physiologically balanced salt solution and one or more anti-oxidative ingredients which collectively confer on said ophthalmic composition a total antioxidant capacity as a FRAP value of greater than 100 μmol / l, preferably greater than 150 μmol / l. The composition also has peak UV absorption in the range of 200-350 nm. Suitable anti-oxidative ingredient includes ascorbate (ascorbic acid), urate (uric acid), cysteine, glutathione, tyrosine, lactoferrin, transferrin, albumin, caeruloplasmin and lacrimal gland peroxidase.

A method for increasing the longevity of an old animal comprising administering to the animal a composition comprising one or more antioxidants in a total antioxidant amount sufficient to increase the longevity of the animal.

The invention relates to a preparation method of functional wheat bran, which comprises the steps of removing 1%-4% of bran component a of wheat with a wipe-type wheat peeler, continuing to peel, obtaining a bran component b accounting for 5%-10% of wheat grains, adding alkaline liquor to the bran component b till the moisture content of materials reaches 15%-50%, uniformly mixing, conducting solid alkaline hydrolysis for 2-48hr at 20-60 DEG C, extruding and expanding the materials after the alkaline hydrolysis with a twin-screw extruder, and preparing the functional wheat bran through low-temperature superfine grinding, size mixing, homogenizing, spray drying or drum drying. Compared with the common bran, the functional wheat bran has higher antioxidant activity; the content of available phenolic acid of the wheat bran is increased by 3-6 times; the DPPH (1,1-diphenyl-2-picrylhydrazyl) radical scavenging capacity is increased by 3-5 times; and the total antioxidant capacity of the bran is increased by 3-4 times.

The invention relates to preparation and application methods of penaeus monodon fermented feed. The preparation method comprises the following steps: respectively carrying out enlarged culture on bacillus licheniformis, lactobacillus plantarum and candida utilis, then mixing in a volume ratio of 1:1:1, adding brown sugar (2g / 1000mL) and running water into the mixed bacterial solution under the condition that the ratio of the culture solution to water is 1:80, uniformly mixing, uniformly stirring with penaeus monodon compound feed in a ratio of 2:5, sealing with a plastic film, and fermenting for 18-28 hours, so that the fermented feed is obtained. The invention also provides the application method of the fermented feed. The prepared fermented feed is used for feeding penaeus monodon once a day, and the feeding amount is 20-30% of the daily total feeding amount. The prepared fermented feed can obviously improve nitric oxide synthase activity, superoxide dismutase activity, catalase activity, phenoloxidase activity, glutathione peroxidase activity and total antioxidant capacity of penaeus monodon and obviously reduce the malondialdehyde content; and composition of florae in intestines of the penaeus monodon is obviously improved, and the survival rate of penaeus monodon culture is obviously increased.

The invention discloses Oudemansiella canarii and application thereof. The strain number of Oudemansiella canarii is JZB2115055, and the accession serial number of Oudemansiella canarii in the China General Microbiological Culture Collection Center is CGMCC No.6171. The IC50 of the scavenging rate of Oudemansiella canarii flavone extract on DPPH free radicals is 1.73 micrograms / ml, the IC50 of the scavenging rate of Oudemansiella canarii polysaccharide extract on DPPH free radicals is 593.66 micrograms / ml, the IC50 of the total antioxidant capacity of Oudemansiella canarii flavone extract is 24.74 micrograms / ml, and the IC50 of the total antioxidant capacity of Oudemansiella canarii polysaccharide extract is 240.18 micrograms / ml. Oudemansiella canarii flavone extract and Oudemansiella canarii polysaccharide extract can be developed into an antioxidant or a free radical scavenger.

The invention discloses a preparation method of antioxidant sea-buckthorn polypeptide. The preparation method comprises the following steps: performing cleaning and soaking treatment on sea-buckthorn seeds, and then performing enzymatic hydrolysis extraction for two times by using pepsase; subsequently decompressing, concentrating and drying to obtain the sea-buckthorn seed polypeptide. The determination of total antioxidant capacity (AOV) shows that the scavenging rate of hydroxy free radicals is as high as 91.35 percent, and the scavenging rate of superoxide anions is as high as 64.33 percent when the concentration of a polypeptide solution is 20mg / mL; the antioxidant sea-buckthorn polypeptide has high oxidation resistance and has a broad application prospect in aspects of antioxidant food and cosmetics.

The invention relates to a detection method for obtaining material composition and total antioxidant activity of lonicera confusa. The detection method comprises the following steps: preparing a test solution; preparing a DPPH solution; preparing a series of concentration gradient VC solution as a standard substance, carrying out HPLC-DPPH analysis by taking DPPH as a to-be-scavenged object, and establishing a regression equation of relationship between VC concentration and free radical scavenging rate; and injecting the test solution into the HPLC-DPPH analysis system to obtaining the chemical ingredient information of a sample and the total free radical scavenging rate of the sample, and substituting into the regression equation, so that the total antioxidant activity of the sample is obtained. The detection method provided by the invention has the advantages that an HPLC-DPPH online combination method is adopted, and chemical fingerprint chromatography information and the total antioxidant activity of the lonicera confusa can be obtained at the same time, so that the advantage of carrying out quality control and 'spectrum-effect' evaluation on the lonicera confusa is realized.

The invention discloses an application of inonotus obliquus polysaccharide used as a feed additive for litopenaeus vannamei, and the addition amount of the inonotus obliquus polysaccharide in a litopenaeus vannamei basal feed is 500-1500 mg / kg. The fact that the inonotus obliquus polysaccharide is used as the additive for the litopenaeus vannamei feed can substantially raise the weight gain rate and the specific growth rate of the litopenaeus vannamei, substantially reduce the feed coefficient, meanwhile substantially increase lysozyme and alkaline phosphatase in serum of the litopenaeus vannamei and raise total antioxidant capacity of the litopenaeus vannamei, increase nitric oxide synthetase and superoxide dismutase in the serum, and reduce the content of malonaldehyde in the serum. The inonotus obliquus polysaccharide has an equivalent action effect to beta glucan, and is a new-type immunopotentiator, and has a broad application prospect in the aquaculture field of the litopenaeus vannamei.

The invention aims to provide application of organic dicarboxylic acid salts, namely new application in pharmacy, in particular application in preparing medicaments for preventing and treating cataract. New anti-cataract active components in No.1, No.2, No.3, No.4, No.6, NO.7, No.8 and No.9 compounds are discovered by screening rabbit and rat H2O2 induced cataract and rat xylose induced cataract models, and the medical application of the compounds in preparing the medicaments for resisting oxidative damage and sugar metabolism disorder induced cataract is discovered. The No.4 compound has strong and high effect of resisting phacoscotasmus, and has good medicinal prospect. The No.4 compound has obvious effects of improving rabbit lens water-soluble proteins (sol-pro) and total antioxidant capacity (T-AOC), and reducing malondialdehyde (MDA), so the effect of the No.4 compound of resisting H2O2 induced lens oxidative damage is related to the strong oxidation resistance of the No.4 compound.

The invention discloses an astaxanthin nanoliposome and a preparation method and application thereof. The preparation method comprises the following steps: S1, dissolving astaxanthin, cholesterol andphospholipid in a mixed solution of dichloromethane and trichloromethane, oscillating and fully mixing, decompressing and evaporating to remove an organic solvent so as to form a layer of uniform film, and then drying in vacuum; S2, adding a buffer solution into the film, eluting the film, and rotationally dissolving and uniformly dispersing to obtain an astaxanthin liposome suspension; and S3, sealing the suspension in a dark place at a temperature of 20-30 DEG C for 6-24 hours, and then carrying out membrane filtration by using a liposome extrusion instrument to obtain the astaxanthin nanoliposome. The astaxanthin nanoliposome has the characteristics of excellent characterization characteristic, high entrapment rate, uniform particle size, good dispersibility, good stability and strong antioxidant activity, greatly improves the specific growth rate and survival rate of prawns, the total antioxidant capacity and the immunity, and has the advantages of high synthesis rate, simple process and easy industrialization.

The invention discloses an additive premix with a function of sow oxidative stress reduction. The additive premix comprises a carrier and an additive composition, and the additive composition is prepared from 8-50mg / kg of 4',7-dyhydroxyl isoflavone, 10-25mg / kg of selenomethionine, 300-500mg / kg of ferrous fumarate, 100-400mg / kg of zinc glycinate, 10-1000KIU / kg of vitamin A, 30-70mg / kg of vitamin Eand 1-20mg / kg of vitamin C. The invention further provides a utilization method of the additive premix, adding the additive premix formed by a composition composed of biological activate substances ismore effective than adding any single component, and accordingly consumption of each additive is reduced, and cost saving is realized. By adoption of the additive premix, total antioxidant capacity of sow plasmas can be remarkably increased, sow lipid peroxidation degree is lowered, sow oxidative damage resistance is improved, and accordingly sow oxidative stress reduction is reduced.

The invention relates to a kit for detecting total antioxidant capacity. The kit comprises an R1 reagent and an R2 reagent with a volume ratio of 11:1, wherein the R1 reagent comprises a liquid A and a liquid B with a volume ratio of 10:1; the liquid A comprises acetate and the balance of double distilled water; the liquid B comprises a complex TPTZ (tripyridyl-triazine), acid and the balance of double distilled water; and the R2 reagent comprises an oxidizing agent FeCl3 and the balance of double distilled water. According to the invention, by adopting an FRAP (Ferric Reducing Antioxidant Power) method, the total antioxidant capacity of a sample to be detected is detected, the total antioxidant capacity of a reaction organism can be reflected, and the antioxidant state can be well reflected compared with that of the single antioxidant measurement. By using a spectrophotometer, a microplate reader or fully automatic biochemical analyser, the kit is high in automation and is beneficial to the popularization of the daily inspection work. A measurement value is high in dependency degree with in vivo test data. The kit is low in manufacture cost.

The present invention discloses a taurine compound preparation for groupers and an application thereof. The taurine compound preparation is composed of the following raw materials in parts by weight: 30-70 parts of taurine, 10-40 parts of alanyl-glutamine, 2-6 parts of vitamin C polyphosphate, 1-5 parts of vitamin E and 4-19 parts of corn starch. The taurine compound preparation for the groupers has the advantages of promoting the growth and feed conversion efficiency of the groupers, reducing the ratio of liver weight and body weight, reducing the deposition of body fat and liver fat of the groupers, can significantly improve the activity and total antioxidant capacity of superoxide dismutase, catalase, serum antioxidant enzymes and glutathione peroxidase in liver and plasma of saddletail groupers, and at the same time significantly reduces the malondialdehyde content.

A direct comparisons of the antioxidant activities of various pure compounds (eg., Trolox®(6-hydroxy-2,5,7,8-tetramethylchroman-2-carboxylic acid), extracts and biological fluids (eg. human plasma) can be effected by means of standardisable, objective method of determining and measuring the antioxidative and oxidative radical scavenging activities of a natural or synthetic substance, including the measurement of an indicator reaction product gas, ethylene, in the reaction headspace using Selective Ion Flow Tube Mass Spectrometry (SIFT-MS). This method assays ethylene liberated from α-keto-γ-methiolbutyric acid (KMBA) on reaction with peroxy radicals, other radicals or other reactive oxygen species that can oxidise KMBA to ethylene. From the production rate and concentration of the ethylene, the total antioxidant activity of an added analyte in question and its rate of reaction with oxidative free radicals may be determined.

The invention discloses an application of 2,6-di-tert-butyl-4-methylphenol as a plant activator on apple fruits, and belongs to the technical field of plant induced disease resistance. A 2,6-di-tert-butyl-4-methylphenol solution with the concentration of 0.1 mmol*L<-1>-0.2 mmol*L<-1> can be used for inducing apple fruit disease resistance and preventing and treating apple diseases. The 2,6-di-tert-butyl-4-methylphenol solution has no isolated bactericidal or bacteriostatic effect in itself, can induce plant disease resistance under living body conditions, and the drug resistance produced by induction has the characteristics of long persistence and broad spectrum, and 2,6-di-tert-butyl-4-methylphenol is environmentally friendly and safe and does not suitably produce drug resistance. 2,6-di-tert-butyl-4-methylphenol has the advantages of simple use method, low concentration, low amount and low cost, and can also improve the activity of defense enzymes in plants and total antioxidant capacity.

The invention relates to a preparation method of five-cereal yoghourt. The five-cereal yoghourt is prepared by adding black sesames, black soybeans and corns as five-cereal raw materials into milk, and carrying out homogenizing, sterilizing, inoculating, fermenting and other steps on the five-cereal raw materials. The yoghourt prepared by adopting the method has the characteristics of uniform color, fine and smooth texture, no lamination, proper thickness and the like, has a flavor of corn and fresh scent of the black sesame, is relatively good in flavor, and relatively long in storage period. The five-cereal yoghourt disclosed by the invention is relatively strong in oxidation resistance; and the total antioxidant capability, the DPPH free radical scavenging rate, the hydroxyl free radical scavenging rate, the reducing capability, and the superoxide anion free radical scavenging capability are enhanced along with the enhancement of the mass concentration of the yoghourt.

The invention belongs to the field of pharmacy and relates to a new application of a traditional Chinese medicine motherwort extract in pharmacy, in particular to the application of the purified motherwort extract in preparing nerve protection medicine for treating cerebral injury, especially the application of the purified motherwort extract in preparing brain protection medicine for treating artery obstruction in the brain. By an animal experiment of the purified motherwort extract, a result indicates that the brain stem volume is obviously reduced after the purified motherwort extract is applied, the obstacle grading of the nerve function is decreased by being compared with a contrast group, the total antioxidant concentration in plasma is increased, the DNA oxidation injury is reduced, the expression levels of apoptosis and apoptosis promoting protein are reduced, and the content of apoptosis resistance protein is also increased. The purified motherwort extract can be used for preparing the nerve protection medicine for treating the cerebral injury and can effectively treat cerebral apoplexy.

The invention belongs to the field of medicine, and particularly relates to a method for screening and quantifying ABTS<+>-CE-DAD of antioxidant active components in traditional Chinese medicine. Taking Shuxuening as an example, specific to the aim of verifying the feasibility of the method, nine types of antioxidant active components in Shuxuening are screened and determined. In an experimental process, reaction conditions such as the pH value and phosphate concentration of a buffer solution, the concentrations of sodium dodecyl sulfate and beta-cyclodextrin, the content of acetonitrile, the temperature and the voltage are optimized. Under the same condition, ABTS+ and a Shuxuening injection sample are sampled in sequence, and components with antioxidant activity are reacted with ABTS+ and detected separately. Meanwhile, the certain detected antioxidant active components can be quantified by the determined conditions, and the total content of the active components in a sample is found to be linear with total antioxidant activity. As proved by the result of methodology, the method can be used for screening active compounds in traditional Chinese medicine on line, is easy, convenient, reliable, low in energy consumption, environmentally friendly, and easy for automation.

The invention relates to a method for measuring the total antioxidant capacity of small berries, provides an ORAC detection kit suitable for the determination of the total antioxidant capacity of small berries and a detection method thereof, and belongs to the technical field of activity detection and determination. The kit includes: antioxidant standard substance Trolox storage reagent, fluorescent substance fluorescein sodium storage reagent, free radical generator AAPH reagent, phosphate buffer, and fluorescent microwell reaction plate, which is made of a plastic or hard paper material Boxes are packed together to make up. The ORAC detection method for the total antioxidant capacity of small berries comprises the following steps: small berry sample pretreatment; detection with reagent kits; and analysis of detection results. By mixing the sample and the reagent in a certain volume ratio, the fluorescence microplate analyzer is used to detect the change of the fluorescence intensity of the complex, thereby measuring and calculating the total antioxidant capacity of the small berry. The invention can provide a complete set of total antioxidant capacity test of small berries, has high sensitivity and good accuracy, and is convenient for popularization and application.

The invention belongs to biotechnology pharmacy technology field. The recombination plasmid Pci-Hpon3 expressed in zooblast is constructed by used human mintacol enzyme 3 gene (Hpon3), Hpon3 is expressed efficiently and secreted into blood in small murine skeletal muscles organizing in electric transferring method to improve PON3 in blood serum and enforce the ability of small mouse antioxidation pressure so that it can control liver damage efficiently. The activity of Hpon3 transgene expression small mouse blood serum hydrolytic decomposition melilotol is improved by 1.4 times and can maintain 24 days. After small mouse acute or subacute liver damage mould is built by injecting carbon tetrachloride into abdominal cavities of transgene group and normal small mouse, the blood serum ALT and AST level of transgene small mouse are lower greatly, malondialdehyde (MDA) level of liver homogenate is lower, reducing glutathione (GSH) and total antioxidant ability (T-AOC) are higher than contrasting group, and transgene group small mouse liver cell damage extent indicated in nosology slicer result is lower than CC1(4) control group's indicate that recombination human PON3 plasmid transgene expression can improve efficiently blood serum PON3 activity and reduce liver oxygenizing pressure so that it can be used for preventing and treating liver damage.

The present invention relates to a kind of anti-senility plaster. Magnetic powder, rhodiola root, corydalis tuber, cassia, asarum herb, Kansui, licorice, musk, borneol and ginger juice in certain proportion are prepared into the plaster; and the plaster is adhered to corresponding acupoint. The plaster can eliminate free radical, lower the lipid peroxide level and strengthen SOD activity and total antioxidant capacity, and has the effects of warming and dredging meridian, promoting blood circulation to disperse blood clots, invigorating spleen and kidney, regining, resisting senility, etc. Needing no oral taking, the plaster has reduced liver burden, no toxic side effect, convenient use a fast acting.

The invention discloses a chitooligo saccharide piglet feed, and belongs to the technical field of pig feeds. The chitooligo saccharide piglet feed is prepared from a basic feed and a feed additive, wherein the feed additive is chitooligo saccharide; the basic feed is prepared from corn meal, bean pulp, wheat bran, stone powder, rice bran, peanut meal, fish meal, calcium hydrogen phosphate, edible salt, glucose, amino acid, zeolite powder, composite vitamin for pigs and composite trace elements for the pigs. The feeding method for the chitooligo saccharide piglet feed is simple, and the chitooligo saccharide piglet feed is safe, nontoxic, reliable and low in cost; the feed conversion ratio of weaned pigs can be effectively increased, and the diarrhea rate can be decreased; the total antioxidant capacity in serum is improved, the contents of glutathion peroxidase, superoxide dismutase and catalase are increased, and the content of malonaldehyde is decreased; therefore, the chitooligo saccharide piglet feed is favorable for overall healthy and sustainable development of the pig industry.

The invention provides a preparation method of antrodia camphorata conidial powder and antrodia camphorata grain powder. The preparation method has the advantages that due to the fact that the fermentation process is simple and high in efficiency, a large amount of antrodia camphorata conidial powder can be obtained, large-scale production can be achieved easily, and the method has important industrial application value; the intermediate products of the prepared antrodia camphorata conidial powder can be dried and crushed to obtain the antrodia camphorata grain powder with antrodia camphorata fragrance and good total antioxidant activity.

The present invention discloses a preparation method of green plum wine combined with glutinous rice saccharification fermentation. The preparation method comprises the following steps: 1, high-quality green plums are selected, the selected green plums are washed, and the washed green plums are drained; 2, the drained green plums are frozen; 3, the frozen green plums are thawed and the thawed green plums are squeezed into juice to obtain green plum juice; 4, glutinous rice is soaked, the soaked glutinous rice is steamed, the steamed glutinous rice is sprayed to be cooled to 30-32 DEG C, the cooled glutinous rice is put into a cylinder, rhizopus is added, hole-digging saccharification fermentation is conducted for 44-48 hours, the green plum juice and water for wine-brewing are added, initial sugar content of fermentation liquid is controlled at 20-23%, and pH is 3.2-3.5; 5, acid-lowering yeasts are added; 6, main fermentation is conducted; 7, wine body and wine residues are quickly separated; 8, the wine body is clarified with a clarifying agent and the clarified wine body is put still at room temperature for 3 days; and 9, filtering and wine heating for sterilization are conductedto obtain clarified golden wine liquid. The green plum freezing and juice squeezing, glutinous rice saccharification and green plum wine acid-lowering fermentation are combined to obtain the green plum wine which is golden in wine liquid, has a mellow green plum flavor, and is full in the wine body, pure in mouthfeel and high in total antioxidant capacity.

The invention provides a preparation method of silkworm pupa protein antioxidant peptides and belongs to the technical field of food processing. The preparation method comprises the following steps: 1) mixing a silkworm pupa powder and petroleum ether and performing ultrasonic treatment and solid-liquid separation; 2) mixing a degreased silkworm pupa powder, water and silkworm pupa proteolytic enzymes for enzymolysis; 3) performing enzyme deactivation on a silkworm pupa powder enzymatic hydrolysis solution, centrifuging, filtering the obtained liquid supernatant by adopting a filter membrane of 0.40-0.50 mum and collecting a filtrate; 4) performing ultra-filtration on the filtrate by adopting an ultra-filtration membrane of 3000 Da and collecting a permeate to obtain a ultra-filtrate; 5) performing ultra-filtration on the ultra-filtrate by adopting an ultra-filtration membrane of 200 Da and collecting an intercept object. In the silkworm pupa protein peptides prepared through the preparation method provided by the invention, the content of essential amino acids reaches about 43 percent, and the total antioxidant ability, the ability to scavenge superoxide anions (O2.), the abilityto scavenge .OH and the ability to scavenge DPPH are stronger.