The invention discloses sucrose waste molasses aquiculture feed and a preparation method thereof. The sucrose waste molasses aquiculture feed consists of the following components in parts by weight: 4-60 parts of sucrose waste molasses, 4-20 parts of rice bran or wheat bran, 0-16 parts of starch, 24-26 parts of soybean meal, 18-24 parts of rapeseed meal, 13-16 parts of fish meal, 8-12 parts of meat and bone meal, 2-3 parts of soybean oil, 0.8-1.2 parts of calcium dihydrogen phosphate, 0.15-0.20 parts of choline chloride, 0.8-1.2 parts of complex minerals and 0.1-0.3 parts of compound vitamins. The preparation method comprises the steps of using water-absorbing biological raw materials, i.e. the rice bran, bran meal or and / the starch as water absorbing raw materials, making high-viscosity sucrose waste molasses into solid molasses powder, adding other feed raw materials, evenly mixing, adding water, granulating and screening to obtain the aquiculture feed. When the sucrose waste molasses fully replaces the starch, the intake, the specific growth rate and the feed benefit to protein benefit ratio are obviously increased (p is less than 0.05), the cost of the aquiculture feed is reduced, the rich byproduct resources in the sugar industry in China are reasonably used, the economic benefit of the sucrose waste molasses is improved, the grain resources are saved, and the economic benefit, the social benefit and the environmental benefit are better.