321 results about "Specific growth" patented technology

A method for producing the high-value ketocarotenoid astaxanthin by the green microalga Chlorella zofingiensis in dark-heterotrophic cultures shows excellent growth and high-yield astaxanthin production on glucose-supplemented media in the dark. The specific growth rate and astaxanthin yield can be as high as 0.031 h−1, and 10.3 mg l−1, respectively, which are the highest so far reported in heterotrophic algal cultures. The light-independent astaxanthin-producing ability of Chlorella zofingiensis can be employed for commercial production of astaxanthin using industrial fermenters.

The present invention discloses a method of cross-linking amnion to be an improved biomedical material. The present invention adopts the amnion cross-linked by EDC (N-(3-dimethylaminopropyl)-N′-ethyl-carbodiimide HCI) or NHS (N-hydroxysuccinimide), and the cross-linked amnion not only has more resistance to protease, but also binds specific extracellular matrix (ECM) such as heparin by using cross-linked functional group. Further, by using the affinity of the ECM with specific growth factors, the amnion can be an efficient carrier for specific growth factor. Hence, some specific diseases may be treated.

A culture system for maintaining avian PGCs for long periods in tissue culture is provided. This culture system uses LIF, bFGF, IGF and SCF. The resultant PGCs are useful for the production of transgenic and chimeric avians, in particular, chickens or turkeys.

The invention discloses a method for preparing an ultra-thin porous lamination gradient composite support of tissue engineering, belonging to the field of biomedicine material. The preparation method comprises the following steps: (1) determining materials and structures of a support to be prepared according to the condition of repair and defection; (2) preparing each layer of the support, waiting for the volatilization of a solvent, and gradually drying slurry for forming; (3) placing a formed object to a ventilation place, and demoulding the formed object after the residual solvent is completely volatilized; and (4) taking out the formed object and dry to obtain the ultra-thin porous lamination gradient composite support of the tissue engineering. The ultra-thin porous lamination composite support, the thickness of which is suitable for articular cartilage repair, can be prepared by using the method. The prepared support has favorable mechanical property, favorable biocompatibility and controllable degradation rate, is suitable for co-culture of osteoblast and chondrocyte and compound of specific growth factors, and can repair the synthetic defection of the joint cartilage tissue and the cartilage / bone tissue.

Disclosed are: a culture medium containing a specific growth factor and at least one phospholipid; a composition for preparation of the culture medium; a kit; and a method. A technique can be provided which uses a serum-free or low-serum culture medium and has a promoting effect on the proliferation of an animal cell comparable to the promoting effect obtained by the culture in a serum-containing culture medium.

The present invention describes a method of optimizing CO2 concentration to increase the specific growth rate of Anammox bacteria and methanogens in wastewater and sludge treatment, as well as novel systems and methods of treating wastewater and sludge. The specific growth rate or doubling time of the Anammox bacteria and methanogens were determined to be sensitive to dissolved CO2 concentration. Optimizing dissolved CO2 concentration increases the specific growth rate of the Anammox bacteria, which may be used as an alternative biological nitrogen removal process for the treatment of domestic wastewater. In the method and system of treating sludge, the CO2 stripper returns biogas with low CO2 concentration to the headspace of an anaerobic digester in order to lower the headspace CO2 concentration and therefore, the soluble CO2 concentration. The lower soluble CO2 concentration increases the specific growth rate of the methanogens for a more efficient anaerobic digestion process.

The invention belongs to the bait research and exploitation technical field in fishery science. The invention relates to an optimum processing technology for a compound Chinese herbal medicine additive aquatic feed. By using different processing technology and component proportion on the compound Chinese herbal medicine, the compound Chinese herbal medicine can be taken as one of feed raw materials with low investment and high yield, the aquatic feed can effectively increase the feeding of the fish, absorption efficiency and assistant growth and the like, and can be taken as a novel feed resource for exploitation and application. The compound Chinese herbal medicine feed additive can be prepared by the following steps according to different processing technologies: inspecting medicines, weighting, using a water extraction or a crushing method, adding a formula feed, uniformly mixing, placing under the temperature of 30-38 DEG C for continuously fermenting for 1-2 hours to prepare the granular feed, and drying and moulding at the temperature of 60 DEG C. By culture tests, compared the test feed with the basic feed, the weight gain, specific growth rate, bait coefficient, feeding promotion activity equalization index of the tested fish can be tested, and the optimum processing technology and the component proportion can be determined. The research has important application meaning.

A method for producing the high-value ketocarotenoid astaxanthin by the green microalga Chlorella zofingiensis in dark-heterotrophic cultures shows excellent growth and high-yield astaxanthin production on glucose-supplemented media in the dark. The specific growth rate and astaxanthin yield can be as high as 0.031 h−1, and 10.3 mg l−1, respectively, which are the highest so far reported in heterotrophic algal cultures. The light-independent astaxanthin-producing ability of Chlorella zofingiensis can be employed for commercial production of astaxanthin using industrial fermenters.

The invention provides an anti-rice black-streaked dwarf virus field inoculation identification method for rice varieties, and belongs to the technical field of agricultural science. A piece of rice field with certain conditions is selected as an identification nursery, different rice varieties are sown in special stages, it is identified that virus transmission interbody small brown rice planthoppers in the nursery have certain virus carried rate and population density after wheat harvest, and after rice grows to a specific growth period, the anti-rice black-streaked dwarf virus level of the rice varieties is evaluated according to the severity degree of disease. With the method, anti-rice black-streaked dwarf virus identification can be performed on a large number of rice varieties at a time, and the defects that the manual identification scale in a laboratory and the identification variety number are small are overcome. The identification method can be applied to the multiple fields of screening of anti-rice black-streaked dwarf virus rice varieties, variety resistance evaluation, resistant variety breeding, researching on the law of genetics of resistance and the like.

The invention discloses a database table space monitoring method. The method comprises the steps that a specific growth period and an alarm threshold valve of minimum available days of database table space are set in advance; the using conditions of a daily table space data volume is acquired regularly; a latest period serves as an acquisition cycle, the daily data growth amount during the acquisition cycle is extracted, and a reference value of the daily growth amount is calculated; the number N1 of the available days of a surplus capacity is calculated according to the surplus size of the current table space and the reference value of the daily growth amount; a judgment is conducted on the number N1 of the available days of the surplus capacity to see if the specific growth period is included, and the available days of the surplus capacity are adjusted; the table space with the available days of the surplus capacity reaching the alarm threshold valve is subjected to early warning. According to the database table space monitoring method, the table space usage rate information can be automatically transformed into the available days accurately by dividing the database table space growth into a normal business data growth period and the specific growth period, so that effective assessments of the daily business data growth amount and the available days of the surplus capacity of all the current table space are achieved.

The invention provides a recombinant saccharomyces cerevisiae strain for continuously and efficiently secreting beta-glucosidase, which is named Saccharomyces cerevisiae 102SB, and preserved with a Preservation No. of CGMCC No. 7450 in the China General Microbiological Culture Collection Center on April 11, 2013. The recombinant saccharomyces cerevisiae disclosed by the invention can continuously and efficiently secrete beta-glucosidase in a complex non-selective medium, and the extracellular enzyme activity can reach 1005.3 U / g (dry weight). By using a characteristic that the maximum specific growth rate of cellobiose is consistent with that of glucose, the strain reaches 0.29 h<-1> under the condition of limited oxygen. In SSF taking a cellulose material as a substrate, the yield of ethanol taking microcrystalline cellulose as a substrate is increased by 110%, and the yield of ethanol taking acid-hydrolyzed corncobs as a substrate is increased by 89%. The recombinant saccharomyces cerevisiae strain disclosed by the invention is of great importance in reducing the production cost of simultaneous saccharification and fermentation in the process of cellulosic ethanol production, and simplifying the production process.

The invention provides a method for producing pectinase by fermentation of recombinant Escherichia coli, which belongs to the technical field of fermentation engineering. According to the invention, Escherichia coli BL21DE3 is used as an original strain, and a glycerin medium is fed in batches according to a certain specific growth rate. The method provided in the invention is simple and practicable, substantially improves output, production intensity and secretory expression efficiency of pectinase, is favorable for large scale industrial production, and has important significance in guiding secretory expression level of foreign proteins in other Escherichia coli expression systems.

The invention relates to a high-yield cultivation method for peanuts, which belongs to the technical field of agricultural cultivation. The method comprises the following steps: A, land selection; B, seed treatment; C, sowing; D, fertilization time and mode; and E, seedling cultivation. By using the high-yield cultivation method for peanuts disclosed by the invention, the fertilization method for peanuts is rational and scientific, the soil property is improved, and the loss of inorganic fertilizers can be reduced; different sowing depths are adopted according to the difference among specific sowing modes, so that the specific growth conditions of peanuts are met; meanwhile, a wheat-row interplanted sowing form which is a cultivation mode designed for improving the multiple cropping index and fully using the soil fertility and light energy resources is adopted, so that the land utilization rate is improved, and the yield of peanuts is improved; and the seedling cultivation process is rational and scientific and meets the needs of peanut growth, so that obtained peanuts have an optimal quality and yield.

The invention discloses sucrose waste molasses aquiculture feed and a preparation method thereof. The sucrose waste molasses aquiculture feed consists of the following components in parts by weight: 4-60 parts of sucrose waste molasses, 4-20 parts of rice bran or wheat bran, 0-16 parts of starch, 24-26 parts of soybean meal, 18-24 parts of rapeseed meal, 13-16 parts of fish meal, 8-12 parts of meat and bone meal, 2-3 parts of soybean oil, 0.8-1.2 parts of calcium dihydrogen phosphate, 0.15-0.20 parts of choline chloride, 0.8-1.2 parts of complex minerals and 0.1-0.3 parts of compound vitamins. The preparation method comprises the steps of using water-absorbing biological raw materials, i.e. the rice bran, bran meal or and / the starch as water absorbing raw materials, making high-viscosity sucrose waste molasses into solid molasses powder, adding other feed raw materials, evenly mixing, adding water, granulating and screening to obtain the aquiculture feed. When the sucrose waste molasses fully replaces the starch, the intake, the specific growth rate and the feed benefit to protein benefit ratio are obviously increased (p is less than 0.05), the cost of the aquiculture feed is reduced, the rich byproduct resources in the sugar industry in China are reasonably used, the economic benefit of the sucrose waste molasses is improved, the grain resources are saved, and the economic benefit, the social benefit and the environmental benefit are better.

The invention discloses a cutinase producing gene engineering bacteria and use thereof and belongs to the technical field of bioengineering. Recombinant plasmid Tfu-0883-hlyAs / pET20b(+) is constructed, Escherichia coli E.coliBL21(DE3) is converted to obtain recombinant Escherichia coli Tfu-0883-hlyAs / pET20b(+) / E.coliBL21(DE3). A certain specific growth speed is controlled in a feed supplement batch fermentation manner, fermentation culture is performed for 30 to 34 hours, and the enzyme survival rate in fermentation supernate reaches 700 to 750U / Ml. In the invention, glycerol is used as a main raw material, a semisynthetic culture medium is adopted, and the cutinase producing gene engineering bacteria have the advantages of stability, convenient regulation and the like and are suitable for large-scale production.

The invention relates to a method of making pluripotent stem cells that does not involve the formation of early preimplantation embryos or fetal tissue. The method has general utility in the production of pluripotent stem cells from many mammalian species but has particular application in man where pluripotent stem cell production can be customized to particular human individual. The method involves the fusion of donor somatic or stem cells (or their karyoplasts) with cytoplasmic, membrane-delimited fragments of mammalian oocytes or zygotes. After the initial genomic reprogramming occurs, the cells can proliferate and thus multiply in vitro yielding a large number of autologous cells for cell therapy application. The result of this process is a cell population genomically identical to the somatic, differentiated cells derived from an individual patient. However, these cells are pluripotent in that upon application of specific growth factors, the cells are capable of differentiating into specific cell types as required by the sought clinical indication.

The invention discloses special feed for a juvenile alligator snapping turtle, which comprises the following components in percentage by weight: 49 percent-70 percent of fish meal, 8 percent-30 percent of bean pulp and / or meat and bone meal, 9.8 percent-23.2 percent of starch, 0.2 percent-4.7 percent of fish oil, 0 percent-6 percent of monocalcium phosphate, 0.5 percent-1 percent of vitamin, 1 percent-2 percent of mineral substance and 0.1 percent-0.3 percent of choline chloride. In the invention, the special feed for the juvenile alligator snapping turtle has the advantages of excellent raw materials and scientific nutrition preparation and can ensure that the raw materials are completely ingested by the juvenile alligator snapping turtle without generating aquaculture pollution caused by waste feed, wherein the crude protein is more than or equal to 45 percent, the crude fat is more than or equal to 6 percent, the water content is less than or equal to 10 percent, and the floating time is up to 8-10 hours; after the part of fish meal is replaced by the bean pulp and / or the meat and bone meal, the survival ratio of the alligator snapping turtle is not influenced, but various indexes, such as terminal weight, specific growth ratio, weight growth ratio and the like are obviously improved, and the production cost is greatly reduced.

Differentiation of human pluripotent stem cells, such as human embryonic stem cells (hESC), into hepatocytes by in vitro methods is disclosed. The pluripotent stem cells are cultured in conditioned medium from the hepatocarcinoma cell line, HepG2. Specific growth factors and defined media may also be added to the medium for stage specific differentiation of the derived hepatocytes. Hepatocytes differentiated from human pluripotent stem cells may be characterized by fluorescence activated cell sorting (FACS), immunofluorescence analysis (IF), real time polymerase reaction (RT-PCR), and functional assays. The methods disclosed herein are able to differentiate high percentages of hepatocytes from human pluripotent stem cells using the disclosed methods. These differentiated cells may exhibit polygonal shape morphology, typical of hepatocytes, and may express hepatocyte specific genes. The differentiated cells may also be positive for definitive endoderm markers and hepatic markers.

In the present invention, a mobile phone judges whether the current environment of a plant is equivalent to a specific season for the plant, based on a detected environment received and acquired from a sensor device including an environment sensor (temperature sensor, moisture sensor, etc.) for detecting a growth environment and the season-specific growth basic information of the plant received and acquired from a server device, and then specifies growth information (growth advice) applicable to the judged season as an output subject.

The invention relates to a preparation method and application of a malignant tumour specific growth factor (TSGF) antigen electrogenerated chemiluminescence sensor. The invention in particular relates to preparation and application of an electrogenerated chemiluminescence immunosensor taking aminated graphene GS-NH2 and an incubation substance of a detection antibody Ab2 as detection antibody labels and provides a simple, rapid and pollution-free preparation method and an application of a nitrogen doped carbon quantum dot electrogenerated chemiluminescence immunosensor. Linear range of the nitrogen doped carbon quantum dot electrogenerated chemiluminescence immunosensor is 0.01ng / mL-100ng / mL, and detection limit is 3.3pg / mL, so that rapid and sensitive detection on malignant TSGF antigen is realized.

The invention relates to a suspension planting method for aquatic plants, which comprises the following steps: (1) manufacturing a suspension planting device for the aquatic plants; (2) adjusting an initial water level of the suspension planting device for the aquatic plants; (3) planting the aquatic plants; and (4) adjusting the height of the water level of the suspension planting device for the aquatic plants at each growing stage of the aquatic plants. The method provided by the invention is simple; the operation is convenient and simple; the cost is low; the initial water level of the planting device can be reasonably controlled and different aquatic plants can be planted; and during a planting process, the height of the water level of the planting device is adjusted to the growth water level normally required by a specific growth stage.

The invention discloses a method for improving the yield of epsilon-polylysine by low pH value stress and belongs to the technical field of fermentation engineering. In a fermentation process, an acid pH stress process is introduced, namely the pH is artificially or spontaneously reduced to 2.5-3.0 and is maintained for 12-48 hours; then the pH is improved to 3.5-4.5 and keeps stable until the fermentation is finished. With the adoption of a pH regulating and controlling method, the specific growth rate of epsilon-polylysine producing bacteria and the specific synthesis speed of the epsilon-polylysine can be remarkably improved; a material supplementing-batch fermentation manner is combined so that the yield of the epsilon-polylysine is improved by more than 50% when being compared with that a common two-phase pH regulating and controlling method. According to the method, a low pH stress method is introduced into an epsilon-polylysine fermentation process so that the fermentation level of the epsilon-polylysine is remarkably improved. Furthermore, the method is simple to operate and has remarkable effect so that the implementation of large-scale industrial production is facilitated.

The invention belongs to the technical field of marine fish (mullet) bait research and development, relates to a treatment method and a component proportion of high-efficiency marine algae enteromorpha for baits, and aims at using enteromorpha as a new feed resource for development and application and applying the enteromorpha as a low-input high-output algae feed in the breeding industry. The marine green tide algae enteromorpha serving as a high-efficiency feed additive comprises the following components: 300g of fish meal, 300g of soybean meal, 70g of whole wheat flour, 50g of yeast, 50mL of maize germ oil, 15g of soybean lecithin, 5g of sodium alginate, 1g of cellulase, 50000IU of vitamin A, 4000IU of vitamin D, 100mg of vitamin E, 50mg of vitamin B1, 50mg of vitamin B2, 5mg of vitamin B6, 10mu g of vitamin B12, 150mg of nicotinamide, 50mg of calcium pantothenate, 500mg of choline bitartrate, 500mg of vitamin C, 100mg of iron, 10mg of copper, 5mg of zinc, 10mg of magnesium, 1mg of iodine, 10mg of manganese, 100mg of potassium, 5580mg of calcium hydrophosphate, and 250mg of L-lysiante, wherein the enteromorpha is added according to a gradient. A feeding attraction activity test is carried out by detecting the rate of live weight growth of mullet, a specific growth rate and a bait coefficient, and the best feed enteromorpha gradient is finally determined. According to the invention, the enteromorpha serving as a low-cost high-efficiency feed additive can be quickly applied in the breeding industry.

A computer and data network assisted automated method and system for determining factors limiting production locally and site-specifically in agriculture or related industrial fields, and for evaluating the mutual significance thereof by means of an expert system. Feedback comprising a solution is furnished via data network to the system of the production site and / or related industry for further measures.

A culture system for maintaining avian PGCs for long periods in tissue culture is provided. This culture system uses LIF, bFGF, IGF-I and SCF. The resultant PGCs are useful for the production of transgenic and chimeric avians, in particular, chickens or turkeys.

The invention discloses a device and method for achieving autotrophic nitrogen removal of low-carbon / nitrogen-ratio sewage based on partial denitrification and anaerobic ammonia oxidation; the device includes a domestic sewage inlet tank, a partial denitrification reactor, an intermediate water tank and an anaerobic ammonia oxidation reactor; the domestic sewage inlet tank is connected with a first water inlet of the partial denitrification reactor through a first peristaltic pump; a first water outlet of the partial denitrification reactor is connected with the intermediate water tank through a second peristaltic pump; the intermediate water tank is connected with a second water inlet of the anaerobic ammonia oxidation reactor through a third peristaltic pump. The invention provides the device and method for achieving autotrophic nitrogen removal of the low-carbon / nitrogen-ratio sewage based on partial denitrification and anaerobic ammonia oxidation, and 50% of the aeration amount can be reduced; an anaerobic ammonia oxidation reaction is a redox reaction, the process is an autotrophic reaction, and thus 100% of a carbon source is reduced; anaerobic ammonia oxidation bacteria are autotrophic bacteria, the specific growth rate is extremely low, the surplus sludge volume is extremely low, and thus the cost of sludge treatment is saved.

The invention discloses a fermentation feeding optimizing control system and a method thereof. The system comprises a fermenting tank, a feeding bottle, a glucose electrode used for monitoring the concentration of sugar in the fermenting tank in real time, an optical density electrode used for monitoring the OD value of thalli in the fermenting tank in real time, an external feeding pump used forpumping a feeding medium, and an external computer control system connected with the glucose electrode, the optical density electrode and the feeding pump, and the running speed of the feeding pump iscontrolled according to the concentration of the sugar, the OD value of the thalli and a preset specific growth rate in order to supplement the feeding medium to the fermenting tank from the feedingbottle in a growth rate control feeding mode and a continuous fed-batch form. The system and the method can improve the automation degree of fermentation feeding, and facilitates the improvement of the fermenting density of the thalli in order to improve the expression of target proteins.

The present invention relates to a fish feed and particularly relates to a glossogobius giuris juvenile fish compound feed. The present invention also relates to a glossogobius giuris juvenile fish compound feed preparation method. The glossogobius giuris juvenile fish compound feed includes the following components in parts by mass: fish meal 17-29 parts, sweet potato vine powder 6-14 parts, green hay powder 7-13 parts, silkworm chrysalis meal 5-12 parts, artemisia capillaris 3-8 parts, lysimachia christinae 2-7 parts, perilla frutescens 2-6 parts, kalimeris indica 3-7 parts, ilex purpurea 2-5 parts, fresh ginger 2-6 parts, toona sinensis 1-5 parts, hibiscus esculentus 1-4 parts, fish oil 2-6 parts, honey 1-4 parts, olive oil 1-4 parts and compound multivitamins 1-4 parts. According to the energy metabolism patterns and nutritional needs of the glossogobius giuris juvenile fish, the present invention provides the glossogobius giuris juvenile fish compound feed to completely replace the fish meal. The glossogobius giuris juvenile fish fed by the compound feed is high in survival rates, low in feed coefficient and high in specific growth rates.

The invention relates to a fish feed and in particular relates to a compound feed for juvenile Pelteobagrus fulvidraco and a preparation method of the compound feed. The compound feed for juvenile Pelteobagrus fulvidraco is prepared from the following components in parts by weight: 21-32 parts of black bean flour, 5-12 parts of rice bran cake, 6-13 parts of rapeseed meal, 2-7 parts of pork powder, 4-9 parts of fructus rosae laevigatae, 5-11 parts of Houttuynia cordata, 3-9 parts of fructus forsythiae, 3-8 parts of Astragalus powder, 3-7 parts of lily root flour, 2-6 parts of sweet potato flour, 2-6 parts of fish oil, 1-5 parts of black garlic powder, 1-4 parts of carrot powder, 1-4 parts of honey, 1-4 parts of corn oil and 1-4 parts of compound vitamin. The compound feed for the juvenile Pelteobagrus fulvidraco is provided according to the energy metabolism mode and the nutritional requirement of the juvenile Pelteobagrus fulvidraco, and can completely replace fish meal, the juvenile Pelteobagrus fulvidraco fed by the compound feed has high survival rate, low feed coefficient and high specific growth rate.