138 results about "Cutinase" patented technology

The present invention relates to polyesterases having cutinase and / or suberinase activity obtainable from Coprinus and Trichoderma. The invention further relates to a method for producing the polyesterases, and to polynucleotides, vectors and host cells used therein. The enzymes are useful in hydrolysing cutin, suberin and other polyesters for example in treating agricultural or food raw materials, or wood raw materials, pulp and paper products and waste, and in modifying polyester fibers, or in laundry and dish applications.

The invention relates to genetically engineered bacteria for efficiently secreting, expressing and reconstructing cutinase and a method for constructing the same, belonging to the field of the bioengineering technology. The genetically engineered bacteria are escherichia coli BL21 (DE3) which carry two recombinant plasmids, and the recombinant plasmids are respectively plasmid pSTV28 carrying the specific genes in the Alpha-hemolysin A (hly A) pathway and plasmid pET20b(+) containing the cutinase-hly As genes. The method for constructing the genetically engineered bacteria for efficiently secreting, expressing and reconstructing cutinase comprises the following steps: constructing two key recombinant plasmids and transforming the constructed recombinant plasmids in the escherichia coli BL21 (DE3) to obtain the genetically engineered bacteria for efficiently secreting, expressing and reconstructing cutinase. The cutinase is produced by using the genetically engineered bacteria through culturing liquid and inducing and expressing the cutinase. The cutinase Tfu_0883 for thermophilic monospore bacteria of Thermobifida Fusca WSH03-11 is used as the report protein. The shaking flask fermentation shows that the extracellular output of the cutinase is 306U / mL which is 1.7 times of the output of the cutinase which adopts the II-type secreting pathway in the preliminary working process in the research laboratory. The cutinase is secreted and expressed efficiently.

The invention relates to the use of certain lipolytic enzymes such as cutinases and lipases in the manufacture of paper and paper products from recycled paper. Examples of such enzymes are derived from strains of Humicola, Candida, Fusarium and Pseudomonas. By use of these enzymes, the problems relating to the so-called “stickies” derived from waste paper are reduced.

The invention relates to the technical field of reuse of waste fungus chaff after edible fungi cultivation and particularly relates to flammulina velutipes fungus chaff feed and a production method and an application thereof. The flammulina velutipes fungus chaff feed comprises a mixture a and a mixture b, wherein the mass ratio of the mixture a to the mixture b is (1000:1)-(1000:10); the mixture a consists of the following raw materials: flammulina velutipes fungus chaff powder, sweet potato dreg powder, rice bran, wheat flour, soya bean meal, fish meal or meat and bone meal and table salt; and the mixture b consists of the following raw materials: cellulase, hemicellulase, aspergillus niger, ligninase, cutinase, feruloyl esterase and brown sugar. The production method of the flammulina velutipes fungus chaff feed is simple. The flammulina velutipes fungus chaff feed is used for culturing barley pests, the time required by culturing the barley pests is short, the weights of the barley pests are heavy, and a plurality of eggs can be laid by the barley pests. The cultured barley pests are added into the conventional feed and are used for feeding beasts and birds, crabs, fishes, turtles, eels, finless eels or batrachia, the growth of a fed animal is obviously accelerated, the increase production is remarkable, and the effect is good.

The invention discloses a wool biological process anti-felting finishing method based on a cutinase, keratinase and protease one-bath process, belonging to the technical field of wool fabric dyeing and finishing, which aims to solve the shortcomings that a wool chlorination anti-felting method causes serious environmental pollution, a single protease method has poor wettability and high felting rate in treating fabric and a cutinase, keratinase and protease three-step method has low treatment efficiency to realize an effect of optimizing wool fabric protease treatment. The method comprises the following steps of: extracting a wool sample to remove free oily impurities; and performing one-bath process treatment in a solution containing cutinase, keratinase and protease of certain concentration, wherein through the synergistic effect of the three enzymes, the hydrophilicity of the fiber scale surface and the acting efficiency of the keratinase and protease in the treatment bath, the removal effect of a scale layer on the wool surface and the anti-felting performance of the wool fabric are improved, and the treatment time is shortened. According to the invention, the biological process anti-felting method can replace the traditional chlorination method, the quality of the wool anti-felting finishing is improved, and protection on ecological environment is facilitated.

A method for scouring and bleaching cotton fabric with composite enzyme preparation in one bath is provided. The method relates to the application of composite alkaline enzyme preparation in scouring and bleaching processes of cotton fabric. The enzyme preparation for scouring and bleaching cotton fabric is compounded from cutinase, alkaline pectase, alkaline xylanase, alkaline cellulose, and carbohydrate oxidase. The method includes: scouring the cotton fabric by adding the composite enzyme preparation, glucose, oxidation bleaching stabilizer RB-3, penetrant JFC, and Triton X-100 in a treating bath at 55-70° C. and pH 8-10, and then bleaching the cotton fabric for 30 min by directly adding tetraacetyl ethylenediamine (TAED) in the scouring bath to activate hydrogen peroxide. After being treated by the method of the present invention, the cotton fabric has high impurity removal ratio, favorable wettability, and high whiteness. The present invention has the advantages of low power consumption, short process flow, and being environment friendly, and may be used to replace conventional chemical treatment process under the condition of concentrated alkali and high temperature.