The invention provides a drink composition containing tea flower, other plant extracts and water. The other plant extracts are selected from chrysanthemum, honeysuckle, lily, plumeria rubra, mesona chinensis, oat or fried oat, barley or fried barley, bamboo leaf flavonoid, ginseng, gynostemma pentaphylla, medlar, haematococcus pluvialis, houttuynia cordata and the like. The invention further provides a preparation method of the drink composition, as well as application of the drink composition in drinks for removing greasiness, clearing away heat and toxic materials, cooling and relieving summer-heat, protecting liver, protecting heart and cerebral vessels, reducing blood fat, reducing blood pressure and treating fatty liver. According to the drink composition provided by the invention, bitterness of the tea flower in a drink is better improved, and good mouthfeel of the drink is guaranteed; besides, beneficial functional nutritional ingredients in herbaceous plants and stability of the drink are improved, so that the quality of products is favorably improved.