935 results about "Endophytic fungus" patented technology

The present invention provides novel microorganisms, compositions and methods of use thereof, for treating, inhibiting or preventing the developing of a plant pathogenic disease and for killing or inhibiting growth of a variety of pests or pathogens. Provided are compositions comprising a novel endophytic fungal organism effective to inhibit the growth of or kill pests and pathogenic microbes, including Ganoderma boninense. Invention compositions are especially useful in preventing and treating basal stem rot in the oil palm, and can be applied on or in the vicinity of the plant or used to sterilize the plant growth medium prior to or concurrent with plant growth therein. The disclosure further provides substantially purified polynucleotides and polypeptides encoded thereby, together with methods of using those products, for example for making transgenic organism.

The present invention is directed to methods and compositions of endophytic fungi that confer stress tolerance to inoculated plants, including both monocots and dicots. In particular, Fusarium species, isolated from the dunegrass, Leymus mollis, growing in plant communities on Puget Sound beaches of Washington State. Upon inoculating a target plant or plant part with the endophytic fungi, the resulting plant shows stress tolerance, particularly drought and salinity tolerance.

This invention provides a novel endophytic fungus, Muscodor, that produces a mixture of volatile antibiotics with activity on specific plant pathogens, bacteria, nemotodes and insects. Also provided is a method for treating or protecting plants, soil and seeds from microbial infections comprising applying an effective amount of a volatile antibiotic producing Muscodor sp. The invention also relates to fungicidal, bactericidal, insecticidal and nematicidal compositions comprising this novel Muscodor strain and the antibiotics and metabolites produced by this strain either alone, or in combination with other chemical and biological pesticides. Also provided is a method for identifying and isolating related gas producing fungi.

The present invention relates to the discovery of a novel endophytic fungus, Muscodor vitigenus, from the liana, Paullinia paullinioides. This fungus produces naphthalene under certain cultural conditions that has chromatographic and mass spectral properties that are identical to authentic naphthalene. In a preferred embodiment, the naphthalene in the gas phase of M. vitigenus is useful in the repellency of unwanted insect pests. The unique biological activity of the novel endophyte suggests a wide range of potential practical applications, particularly in the area of insect repellents, insecticides, antimicrobials, anthelmintics and vermicides.

The invention discloses an endophytic fungi chaetomium globosum strain, a microbial agent and an application thereof. The strain is named as chaetomium globosum strain ND 35, the preservation organization is the China General Microbiological Culture Collection Center, the preservation date is 9th,10,2010, the preservation number is CGMCC No. 4136. The strain is a biological beneficial bacteria with a plurality of biological control effect mechanisms, and has the plant pathogen inhibition capability with broad-spectrum and high-efficiency, and also has capability for promoting a plurality of plants growth, and is suitable for industrial production, the shelf life is long which is suitable for preventing and treating the plant diseases, so that the strain is used for producing the biological bactericide products for preventing and treating a plurality of plant diseases and multifunctional biofertilizer products for promoting the growth of plants.

The invention discloses a separation and selection method of antibiotic antitubercular endophyte, which comprises the steps as follows, the preparation of antibiotic antitubercular plant tissue, the surface sterilization and the sterility test, the separation and the purification of the endophyte, the ferment of endogenetic fungi, the ferment of endogenetic bacterium, the process of the zymotic fluid, the process of the thalli, the selection of the effective antibiotic bacterial strains, the selection of the effective antitubercular bacterial strains and the preservation of the strains. The separation and selection method of antibiotic antitubercular endophyte ensures the realization of obtaining the effective antibiotic antitubercular matter from microorganisms, thanks to separation and selection method, a batch of effective bacterial strains are obtained.

The invention discloses an endophytic fungus in salvia miltiorrhiza bunge. The endophytic fungus is isolated and obtained from the living body of salvia miltiorrhiza bunge by adopting the endophytic fungus isolation and purification technology and is identified to be trichoderma atroviride through microbial taxonomy. The strain was collected in the China General Microbiological Culture Collection Center, with collection number being CGMCC No.4712.. Tanshinone compounds tanshinone I and tanshinone IIA are generated through liquid fermentation of the strain of the endophytic fungus in salvia miltiorrhiza bunge, so the endophytic fungus is an important microorganism for finding the new resources such as the tanshinone chemical components tanshinone I and tanshinone IIA.

This invention relates to the discovery of a novel endophytic fungus, Muscodor albus, which produces volatile antibiotics that have been shown to be effective in the treatment of human and animal waste products. Through experimentation, this invention confirms that Muscodor albus can be used in disposable bags in connection with portable toilets to solve the problem of degradation of waste products in situations where humans are removed from sanitary facilities. This invention includes the discovery of non-volatile inhibitors that are also produced by Muscodor albus and that are similarly effective in treating human and animal wastes. This invention covers a unique and novel method for preparing Muscodor albus for commercial use in the treatment of human and animal wastes, which involves infesting barley seeds with Muscodor albus and then storing them under conditions that will allow the fungus to remain viable for up to nine months.

The invention provides a fermentation method for high cordyceps militaris biomass and high cordycepin content, which belongs to the technical field of bio-engineering. A cordyceps militaris strain is activated, inoculated to a first stage seed tank and further expanded to a fermentation tank, when a certain biomass is achieved in the fermentation tank, a biotic elicitor of Xylaria type endophytic fungus from binkgo biloba extract liquid and non-biotic elicitors of sodium acetate and ammonium sulfate are added for promoting the production of cordycepin in bacteria. The fermentation method adopts an optimized culture medium for effectively increasing the mycelial growth of cordyceps militaris; furthermore, under the regulation role of the elicitors, the cordyceps militaris biomass and the cordycepin content in the bacteria are respectively increased by 1.76 times and 7.14 times compared with the contrast culture medium without the addition of the elicitors and respectively increased by 2.35 times and 23.25 times compared with the ordinary improved PDA. The fermentation method greatly improves the production amount of cordyceps militaris mycelia and the content of the active characteristic ingredient, thereby having broad market prospect.

The invention discloses Paecilomyces variotii bacterial strain SJ1 and in particular relates to sea-buckthorn fungus, a preparation of sea-buckthorn endophytic fungi and extract of the sea-buckthorn fungus and use of the sea-buckthorn fungus extract. By utilizing the Paecilomyces variotii bacterial strain SJ1, growth and root development of crop, such as corn, rice are promoted, antifreezing capacity is improved, and yield is increased. The SJ1 bacterial strain is Paecilomyces variotii according to morphological and genetic analysis and identification.

The invention discloses a huperzia serrata endophytic fungus for producing huperzine A, and belongs to the technical field of biological engineering. The fungus is obtained by separating from fern H. serrata living plant according to the endophytic fungus separation and purification technology, named as shiraia sp. slf-14 by microbial taxonomy authentication and collected in CCTCC on Nov. 6th, 2009 with the collection number of CCTCC No: M 209294. The strain can produce the huperzine A by liquid fermentation. The invention discovers the huperzine A contained in the shiraia sp. fermentation liquid for the first time, and provides a new path for seeking an active huperzine A component resource for treating senile dementia.

The present invention is directed to methods and compositions of endophytic fungi that confer stress tolerance in inoculated plants, including both monocots and dicots. In particular, Curvularia species, isolated from a host grass Dichanthelium languinosum growing in the geothermal zones of Lassen Volcanic and Yellowstone National Parks, confers such stress tolerance. Upon inoculating a target plant or plant part with endophytic fungi, the resulting plant shows stress tolerance, particularly drought and thermal tolerance.

This invention relates to the discovery of a novel endophytic fungus, Muscodor albus, which produces volatile antibiotics that have been shown to be effective in the treatment of human and animal waste products. Through experimentation, this invention confirms that Muscodor albus can be used in disposable bags in connection with portable toilets to solve the problem of degradation of waste products in situations where humans are removed from sanitary facilities. This invention includes the discovery of non-volatile inhibitors that are also produced by Muscodor albus and that are similarly effective in treating human and animal wastes. This invention covers a unique and novel method for preparing Muscodor albus for commercial use in the treatment of human and animal wastes, which involves infesting barley seeds with Muscodor albus and then storing them under conditions that will allow the fungus to remain viable for up to nine months.

The invention relates to the field of biotechnology, in particular to application of alternaria alternate metabolic products in cucumber rhizoctonia solani prevention and control. In the invention, endophytic fungus of camphor trees is separated from camphor tree living bodies collected from Tianmu mountain in Hangzhou, Zhejiang province by adopting endophytic fungus separation technology, and isnamed alternaria alternata 31 through microbial taxonomy identification, and the strain is stored in China General Microbiological Culture Collector Center (CGMCCC) on December 31, 2010, and the collection register number is CGMCC No.4508. The invention has a most obvious characteristic that the strain liquid can be fermented to produce active metabolic products which have inhibiting effect on pathogenic bacteria of plant fungus diseases such as Rhizoctonia solani, Fusarium oxysporium, Botrytis cinerea and the like, thus alternaria alternate is an important microbial resource in agriculture.

The invention discloses endophytic fungi Muscodor sp. The preservation name of the strain is ZJLQ070, the preservation unit is China General Microbiological Culture Collection Center, the preservation date is January 8, 2009 and the preservation number is CGMCC 2864. The endophytic fungi can be prepared into living preparations and can be used for suppressing or killing pathogenic microorganisms or musty microorganisms. The invention simultaneously discloses a fungicide comprising 2-methyl-methyl propionate, 2-methylpropionate and the like, especially comprising alpha-phellandrene, beta-phellandrene, trans1-methyl-4-(1-methyl ethyl)-2-cyclohexene-1-alcohol, cis1- methyl-4-(1-methyl ethyl)-2-cyclohexene-1-alcohol, trans3-methyl-6-(1-methyl ethyl)-2-cyclohexene-1-alcohol and cis3-methyl-6-(1-methyl ethyl)-2-cyclohexene-1-alcohol.

The invention discloses a method for obtaining germination effective symbiotic fungus of Cymbidium bicolor seeds, which includes: using dead twigs, fallen leaves, bark, moss, humus and the like around an original habitant of Cymbidium bicolor as culture medium, subjecting the culture medium and the seeds of Cymbidium bicolor to ex-site symbiotic germination to induce the seeds to germinate and generate protocorms; sterilizing the surface of each obtained protocorm, culturing with thy artificial culture medium under the sterile condition, inducing endophytic fungus in the protocorms to grow, purifying the fungus to obtain a pure colony after mycelia appears; and subjecting the obtained fungus and the seeds of Cymbidium bicolor to symbiotic germination culturing, carrying out a control experiment to check the effectiveness of the obtained fungus to germination of the seeds of Cymbidium bicolor, screening germination effective symbiotic fungus for the seeds of Cymbidium bicolor, and subjecting the fungus to molecular identification and storage. The method is simple to operate, singe variety of fungi can be obtained, massive and troublesome screening is not needed, and a new way of using Cymbidium bicolor seeds and fungus to symbiotically germinate to culture seedlings is opened.

The invention provides a liriope spicate endogenous aspergillus fungus and application thereof to preparation of steroid saponin, and relates to the technical field of microorganisms. The fungus mainly solves the problems that due to the factors that the liriope spicate steroid saponin is mostly and directly from liriope spicate extracts, the liriope spicate steroid saponin content is low, the saponin extraction cost is high, and the like, the market delivery quantity of the liriope spicate steroid saponin is limited. The aspergillus fungus is separated from living body tuberous roots of liriope spicata var. prolifera, and belongs to aspergillus sp. through 18S rDNA sequence identification; the aspergillus fungus is preserved in CCTCC (China Center for Type Culture Collection); the preservation data is December 7, 2016; the preservation number is CCTCC No.M2016721. The liriope spicate produced by a liriope spicate GAP (Good Agricultural Practices) base (the liriope spicate source areain Oumao town, Xiangyang city in Hubei province ) is used; the liriope spicate steroid saponin is generated through liriope spicate endogenous fungus plant liquid fermentation. The endogenous fungus is an important microorganism for finding a new resource of the liriope spicate steroid saponin.

The invention relates to the field of pesticides, in particular to application of a diterpene alkaloid compound serving as a secondary metabolite of a seaweed endophytic fungus. The diterpene alkaloid compound serving as the secondary metabolite of the seaweed endophytic fungus has insecticidal effect and is shown as a formula (I). The natural diterpene alkaloid compound is obtained by extractingand separating yeast of Aspergillus oryzae cf-2 fungus separated from ocean red alga heterosiphonia; and by insecticidal activity experiments, the fatality rate of artemia is 74.2 percent when the diterpene alkaloid compound is 100 micrograms per milliliter.

The invention discloses endophytic fungi from huperzia serrata. The invention is obtained through being separated from the living body of pteridophyta huperzia serrata by adopting endophytic fungi separation and purification technology, and is identified through biosystematics to be acremonium endophytium. The preservation number of the strain is CCTCC M 206118. The invention generates Huperzine A analogous compound through the strain liquid fermentation of the endophytic fungi from huperzia serrata, and is significant micro organism for searching the Huperzine A new resource of the active component for curing the presenile dementia.

The invention discloses an endophytic fungus Phomopsis sp. strain ZJWCF252, which was collected with a name of Phomopsiswenchengensis and a collection number of CGMCCNo.4301 in China General Microbiological Culture Collection Center of Institute of Microbiology of Chinese Academy of Sciences, which is located in building No.3, Yard No.1, Beichen Road, Chaoyang District, Beijing, on November 1st, 2010. The invention also discloses the use of the endophytic fungus Phomopsis sp. in the preparation of 2,3-dihydro-2-hydroxy-2,4-dimethyl-5-trans-propenylfuran-3-one which has strong inhibition effects on various plant pathogenic fungi.

The invention provides a method for preparing a cyclic dipeptides compound as well as an application thereof, the method is to obtain the cyclic dipeptides compound by fermenting, separating and purifying separated endophytic fungi with the Collection No. of CGMCC2094, the compound can effectively induce the system resistance of crop and obviously promote the growth of roots, stems and leaves of plants at different seedling stages, and can enhance the quality of seedlings and promote the formation of strong seedlings, and the method has advantages of convenient and feasible process, non environmental pollution, short period, low cost of materials, easy formation of large-scale batch production and low production cost.

The invention relates to a method for the fast establishment of DSE and plant symbiotic culture system and the application of the method, in particular to a method for the fast establishment of dark septate endophytes and plant symbiotic culture system and the application of the method, belonging to the field of applied microbiology, plant nutrition and ecology. The invention is characterized in that, based on the biological characteristics of DSE of wide carbon utilization and aerobe, riversand with good air permeability is used as the solid substrate; improved MS culture medium is added as the nutrient solution, and early nutritional growth requirements for the formation of DSE-plant symbiotic system are satisfied; the invention utilizes plant seedlings and DSE as materials; by inoculating DSE and plant seedlings on an aseptic culture medium, symbiotic relationship is formed. The method of the invention has the advantages of short setup time of the DSE and plant symbiotic culture system, high efficiency and other excellences; the invention can be applied to the study of the relationship between DSE and plants, the study of DSE morphology, the initial detection of suspected DSE bacterial strains and the application of DSE inoculation.

This invention has clone Stilbene synthase gene (sts) from Parthenocissi Tricuspidatae endogeny eumycete, the coding sequence similarity between Stilbene synthase gene and Parthenocissi Tricuspidatae is 95.25%,this eumycete belongs to Cladosporium sp by 18SrDNA series assessment. High performance liquid chromatogram and mass chromatographic analysis indicate that this eumycete can also composite resveratrol by culture in vitro. The analysis about non- coding part of endogeny eumycete sts gene indicates that: it is very different from regulatory element of sts gene which is in plants. Importing exogenous gene(such as VHb,ipt and iaaM and so on ) in endogeny eumycete can promote endogeny eumycete growing.

The invention relates to plant endophytic fungi of huperzia serrata. The biological classification and molecular biological identification are (Trichodermasp.) L44. The bacterium has been already preserved in China Center for Type Culture Collection, with the preserving number of CCTCC NO: M2013198. The invention further provides an application of the plant endophytic fungi of huperzia serrata in preparing huperzine A. The invention provides a novel microorganism for source of the huperzine A. The microorganism provided by the invention has the advantages of quick growth and reproduction, no limitation on external environments such as seasons and climates and large yield promoting space and the like. The wild resource of the medicinal plant huperzia serrata can be effectively prevented from being excessively excavated.

The invention relates to phytopathology, in particular to endophytic fungi CEF08111 of cotton and an application of the endophytic fungi CEF08111 in prevention and treatment of cotton verticillium wilt. According to the invention, a strain CEF08111 (Gibellulopsis nigrescens) with the collection number of CGMCC No. 5610, which has a significant control effect on the cotton verticillium wilt, is obtained from the endophytic fungi of the cotton by separation, screening, identification and biological assay in a green house. The CEF08111 can be cultured on an artificial culture medium, is easy to obtain and culture, and is conductive to factory production, popularization and application. The CEF08111 has a good control effect on the verticillium wilt; the CEF08111 is firstly inoculated, and verticillium dahliae is inoculated 4 days later, so that the prevention and treatment effect on the cotton verticillium wilt reaches 97.2 percent and the control effect on the verticillium wilt is remarkable; and compared with bacillus subtilis which is more applied to production, the CEF08111 has the advantage that the prevention and treatment effect is improved by about 50 percent, and is safe to people, animals, insects and crops and is environment-friendly.

The invention relates to a method for using endogenous fungus to cultivate saussurea sp, wherein two used endogenous fungus are DSE-1(Mycocentrospora sp.) and DSE-2(Leptodontidium sp.); and the method comprises that cultivating fungus and saussurea seed, then transplanting them into symbiotic substrate to be cultivated. The invention can avoid seed maintain process or shorten said process. And it can avoid hormone and chemical accelerator, to improve the quality and the content of active component.

The invention relates to microbe technique field, endophytic fungus of Chinese Holly of the invention collected from Chinese Holly living plant of Tianmu Mountain of Linan City is obtained by endophytic fungus microbe separation technology, named as Trichoderma harzianum Rifai stem L identified by microbial taxonomy stored by general microbilogy centre of China Forestry Culture Collection Center on date of august 14th 2006, registration number is CGMCC NO.1780. The most prominent feature is the stem can generate activated component seaquiterpenoids Trichothec-9-en-4-ol,12,13-epoxy-,acetate,(4beta)-(8CI,9CI) by fermentation for inhibiting phytopathogenic fungi bacteria, it is an important microbe resource in agriculture.

The invention relates to the technical field of microorganisms, and in particular, relates to salviae miltiorrhizae endophytic fungi which are separated from labiatae salvia plant salviae miltiorrhizae plant living bodies by an endophytic fungi separation and purification technology, are classified and named as chaetomium globosum D68 and have the preservation number of CGMCC No.12622. The invention further discloses an application of the salviae miltiorrhizae endophytic fungi in improvement of the host salvia miltiorrhiza biomass and the content of tanshinones and phenolic acids. The salvia endophytic fungi can effectively improve the salvia miltiorrhiza root biomass through co-culture with salvia miltiorrhiza plants, significantly increase the plant crown breadth, height and single-leaf area, also can effectively promote biological synthesis of tanshinones, phenolic acids and other effective components in salvia miltiorrhiza, and have great application value.

To provide a grass having improved characteristics, an endophyte having endophytic fungi which produce an insect-resistant alkaloid is artificially introduced into a grass by inoculation so that the endophyte lives in and infects the grass.