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227 results about "Elicitor" patented technology

Elicitors in plant biology are extrinsic or foreign molecules often associated with plant pests, diseases or synergistic organisms. Elicitor molecules can attach to special receptor proteins located on plant cell membranes. These receptors are able to recognize the molecular pattern of elicitors and trigger intracellular defence signalling via the Octadecanoid pathway. This response results in the enhanced synthesis of metabolites which reduce damage and increase resistance to pest, disease or environmental stress. This is an immune response known as pattern triggered immunity or PTI. PTI is effective against necrotrophic microorganisms.

Fermentation method for high cordyceps militaris biomass and high cordycepin content

The invention provides a fermentation method for high cordyceps militaris biomass and high cordycepin content, which belongs to the technical field of bio-engineering. A cordyceps militaris strain is activated, inoculated to a first stage seed tank and further expanded to a fermentation tank, when a certain biomass is achieved in the fermentation tank, a biotic elicitor of Xylaria type endophytic fungus from binkgo biloba extract liquid and non-biotic elicitors of sodium acetate and ammonium sulfate are added for promoting the production of cordycepin in bacteria. The fermentation method adopts an optimized culture medium for effectively increasing the mycelial growth of cordyceps militaris; furthermore, under the regulation role of the elicitors, the cordyceps militaris biomass and the cordycepin content in the bacteria are respectively increased by 1.76 times and 7.14 times compared with the contrast culture medium without the addition of the elicitors and respectively increased by 2.35 times and 23.25 times compared with the ordinary improved PDA. The fermentation method greatly improves the production amount of cordyceps militaris mycelia and the content of the active characteristic ingredient, thereby having broad market prospect.
Owner:JIANGSU ACADEMY OF AGRICULTURAL SCIENCES

Phellinus igniarius mycelium liquid fermentation method by utilizing fungal elicitor for improving yield of flavone of phellinus igniarius

The invention discloses a phellinus igniarius mycelium liquid fermentation method by utilizing a fungal elicitor for improving the yield of flavone of phellinus igniarius, which comprises the steps of: inoculating a fungal elicitor strain for activated culture, and then inoculating and culturing a fungal block to obtain a fungal elicitor mycelium through filtration; then drying, grinding and crushing the fungal elicitor mycelium, adding the crushed fungal elicitor mycelium into ethanol for soaking, filtering the obtained solution, collecting filter residue, orderly adding a mixed solution of chloroform and normal butanol, acetone and water into the filter residue for washing, adding trifluoroacetic acid into a dry substance after the air-drying, performing boiling water bath for 0.5 to 2 hours, then filtering the obtained solution, taking a filtrate for neutralization, standing the filtrate, using a microporous filtering film with the pore diameter between 0.4 and 5mu m to filter the filtrate for sterilization to obtain the fungal elicitor, and cryopreserving the fungal elicitor at the temperature of between 10 and 20 DEG C below zero; inoculating a phellinus igniarius strain for activated culture, and then inoculating a fungal block for fermentation culture; and adding the fungal elicitor into the phellinus igniarius to continue culturing to obtain a phellinus igniarius mycelium within 1 to 5 days of liquid fermentation culture of the phellinus igniarius. The method has simple process, low cost and remarkable effect, can greatly improve the content of the flavone of phellinus igniarius cells, and is a production method with industrialization prospect.
Owner:LUDONG UNIVERSITY

Preparation method of triptolide and ramification composition thereof

The invention provides a preparation method of triptolide and ramification and composition of the triptolide. After an elicitor plays a role in tripterygium suspended cell sap, a high yield of triptolide and ramification of the triptolide can be gotten. The extractive gotten by the method has a good effect on inhibiting tumors, a hepatitis b virus and a human immunodeficiency virus (HIV); the extractive can be prepared into soft capsules, or hard capsules, or dropping pills or troches, or dispersible tablets, or granules, or injection, or oral liquid, or suppository which are applied in oral or skin or mucous membrane or rectal way, by that the extractive is used for being solely or being matched with other drugs or adding auxiliary materials which can be acceptable in pharmacy.
Owner:HUAQIAO UNIVERSITY

Soil conditioner as well as preparation method and application of soil conditioner

The invention relates to a soil conditioner as well as a preparation method and application of the soil conditioner. The soil conditioner comprises the components based on parts by weight: 10-15 parts of biochemical fulvic acid, 10-20 parts of polyacrylamide, 5-10 parts of burdock oligosaccharide, 5-10 parts of chitosan, 1-5 parts of zinc sulfate, 1-2 parts of boric acid and 50-70 parts of trichoderma pseudokoningii fermentation liquor. The invention also provides the preparation method and the application of the soil conditioner. According to the invention, biochemical fulvic acid and the polyacrylamide are combined, and trace elements zinc and boron, the disease-resistant elicitor burdock oligosaccharide, chitosan and the biological control fungi trichoderma pseudokoningii which are necessary to plants and deficient in the soil are loaded, so that the soil conditioner having the functions of improving the physicochemical property of the soil, inhibiting the soil-borne diseases, promoting the growth of the plants and inducing the resistance of botanical system is prepared, and is environment-friendly.
Owner:莱阳市圣德生物科技有限公司

Method for producing salidroside by using agrobacterium rhizogenes to inherit and transfer rhodiola sachdlinensis and constructing capillaceous root cultural system

The present invention discloses a method using Agrobacterium rhizogenes (Ar) gene to transform Rhodiola sachalinensis A.Bor to achieve a hairy root system for Salidroside production. By preparation of explant and cultivation in Ar bacteria liquid, Rhodiola sachalinensis A.Bor is induced to grow hairy roots by genetic transformation; the PCR testing proves that hairy roots are generated by transformation; a fermenter is used for massive production of hairy roots, and precursor substances and elicitors are added to improve the Salidroside content. The method is applied to produce Salidroside to substitute the drying-up Rhodiola sachalinensis A.Bor resources.
Owner:JILIN NORMAL UNIV

Tangerine biological antistaling agent based on activity of rhodosporidium and fruit elicitor

The invention discloses a tangerine biological antistaling agent based on the activity of rhodosporidium and a fruit elicitor, which consists of rhodosporidium suspension, gibberellin / kinetin, salicylic acid, auxin and water, wherein each 1 liter of antistaling agent contains 1*1010 to 1*1012 rhodosporidium cells, 50 to 150 milligrams of gibberellin or kinetin, 8 to 100 milligrams of salicylic acid, 10 to 500 milligrams of auxin and the balance of water; and the rhodosporidium is rhodosporidium paludiagenum Fell and Tallman of which the collection number is IMI 394084. The biological antistaling agent can control post-harvest diseases of tangerine fruits effectively on the premise that chemical bactericides are not used.
Owner:ZHEJIANG UNIV

Rooting-promoting, disease-resistant and plant-strengthening agent for cotton and water, fertilizer and pesticide integrated application method

The invention discloses a rooting-promoting, disease-resistant and plant-strengthening agent for cotton and a water, fertilizer and pesticide integrated application method. The rooting-promoting, disease-resistant and plant-strengthening agent is mainly prepared from a growth regulator, a microelement, a signal molecule donor and an elicitor capable of improving the disease resistance of the cotton, wherein the growth regulator is prepared from indolebutyric acid, sodium nitrophenolate, naphthylacetic acid and diethyl aminoethyl hexanoate through compounding, the microelement is prepared from zinc sulfate, ferrous sulfate, ammonium molybdate, amino acid powder and ammonium hydrogen phosphate through compounding, the signal molecule donor is NO donor sodium nitroprusside, and the elicitor is a penicillium dry mycelium extracting solution. The problems that a drip irrigation technology does no good to root development and severe diseases are caused by successive cropping to the cotton are solved, the growth regulator accelerates growth of root systems, the physiological function time of the root systems is prolonged, the disease-resistant elicitor enhances the resistance of cotton plants, growth of lateral roots is further promoted in combination of the signal molecule donor, and the disease-resistant response of the cotton plant is promoted. The rooting-promoting, disease-resistant and plant-strengthening agent can promote growth of roots and resist diseases in multiple angles; the agent can be injected into irrigation water through a fertilizer pump, integrated application of water, fertilizer and pesticides in the drip irrigation cotton field of the northwest inland is realized, and the agent can promote growth of roots, resist diseases and strengthen seedlings and has remarkable disease resisting and yield increasing effects.
Owner:SHANDONG COTTON RES CENT

Elicitor peptides having disrupted hypersensitive response box and use thereof

Disclosed are peptides that induce an active plant response, but not a hypersensitive response, when applied to plant tissue. These peptides also preferably exhibit improved solubility, stability, resistance to chemical degradation, or a combination of these properties. Use of these peptides or fusion polypeptides, or DNA constructs encoding the same, for modulating plant biochemical signaling, imparting disease resistance to plants, enhancing plant growth, imparting tolerance to biotic stress, imparting tolerance and resistance to abiotic stress, imparting desiccation resistance to cuttings removed from ornamental plants, imparting post-harvest disease or post-harvest desiccation resistance to a fruit or vegetable, or enhancing the longevity of fruit or vegetable ripeness are also disclosed.
Owner:PLANT HEALTH CARE INC

Callus culture method of snow lotus herb

The invention discloses a callus culture method of snow lotus herb, comprising the steps of selection and asepsis of plants, induction and culture of callus and large-scale culture. The core technology of the method is selection of the following large-scale culture medium: MS+0-50g / L of cane sugar+0-10g / L of agar+0.1-5.0mg / L of alpha-naphthylacetic acid+0.1-5.0mg / L of 6-benzyladenine. During large-scale culture, an abiotic elicitor, 40-60mu mol / L of SNP+40-60mu mol / L of MJ+40-60mu mol / L of SA is added. The method is easy to operate, and the active ingredient is high in content and low in production cost, does not pollute environment and can reach the level of industrialization. Syringin produced by the culture technology in the invention is invariant, high in content, low in cost and short in cycle and is very competitive in the markets.
Owner:烟台汇鹏生物科技有限公司

Method for producing taxol by using taxus lateral root proliferation

The invention discloses a method of utilizing taxus lateral root proliferation to produce paclitaxel, the steps are that: (1) annual or biennial taxus branches are selected as cutting materials; (2) after the cuttings are sequentially injected into KMnO4 solution, sucrose solution, and plant growth hormone solution for soaking, the cuttings are conveyed to cutting substrate; (3) the cuttings are pulled out after the roots grow up, and are continually conveyed to the cutting substrate after being processed with hormone; (4) a certain quantity of the lateral roots are cut for producing the paclitaxel; (5) being dried under temperature control at the temperature that is less than or equal to 55 DEG C, pure products of the paclitaxel are obtained after processes of grinding, concentrated organic solvent extraction, column chromatography, solvent recovery, crystallizing and drying; or the cut taxus lateral roots are cultured in a improved Murashige and amp;Skoog fluid nutrient medium, bacteriostat is added in the nutrient medium, 2 to 9 days after fresh taxus lateral roots are induced and processed by elicitors, the pure products of the paclitaxel are extracted. The invention provides a new approach for medicine sources of the paclitaxel, and the shortcomings in artificial cultivation of the taxus are solved of low rooting rate, low survival rate after the roots are cut, slow roots proliferation speed, etc.
Owner:HUAZHONG UNIV OF SCI & TECH

Method for hydroponic breeding of huperzine serrate and induced improvement to huperzine a of huperzine serrate

The invention discloses a method for hydroponic breeding of huperzine serrate and induced improvement to huperzine a of the huperzine serrate. According to the method, wild naturalized huperzine-serrate branches with stem tips are used as cuttings, and rapid hydroponic breeding of the huperzine serrate is achieved in a nutrient solution containing hormones and precursor substances with a certain ratio; compared with conventional soil cutting, the method realizes that rooting time is short, the breeding coefficient is large, and the rooting percentage is as high as 98%. The precursor substances enable the huperzine a content of the hydroponic huperzine serrate to be increased by 70% compared with wild plants; the leaf surfaces or / and the roots of the plants are sprinkled with elicitors in a certain concentration, so that a secondary metabolism system of the huperzine serrate is regulated, the huperzine a content of the hydroponic huperzine serrate is increased 1.3-1.6 times, the yield of the huperzine a is increased, and the situation of shortage of huperzine-serrate resources is alleviated to a certain degree. Besides, the method achieves low production cost and a short cultivation period, is not restricted by the natural environment and climate, and has broad application prospects and high popularization value.
Owner:CHANGSHA HUIRI BIOLOGICAL TECH
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