Method for hydroponic breeding of huperzine serrate and induced improvement to huperzine a of huperzine serrate

Abstract

The invention discloses a method for hydroponic breeding of huperzine serrate and induced improvement to huperzine a of the huperzine serrate. According to the method, wild naturalized huperzine-serrate branches with stem tips are used as cuttings, and rapid hydroponic breeding of the huperzine serrate is achieved in a nutrient solution containing hormones and precursor substances with a certain ratio; compared with conventional soil cutting, the method realizes that rooting time is short, the breeding coefficient is large, and the rooting percentage is as high as 98%. The precursor substances enable the huperzine a content of the hydroponic huperzine serrate to be increased by 70% compared with wild plants; the leaf surfaces or / and the roots of the plants are sprinkled with elicitors in a certain concentration, so that a secondary metabolism system of the huperzine serrate is regulated, the huperzine a content of the hydroponic huperzine serrate is increased 1.3-1.6 times, the yield of the huperzine a is increased, and the situation of shortage of huperzine-serrate resources is alleviated to a certain degree. Besides, the method achieves low production cost and a short cultivation period, is not restricted by the natural environment and climate, and has broad application prospects and high popularization value.

Description

technical field [0001] The invention relates to a method for increasing the production of medicinal plants and their secondary metabolites, in particular to the rapid propagation of the hydroponic Melaleuca tower and the induced increase of the content of huperzine A. Background technique [0002] Melaleuca, also known as Huperzia serrata, is a precious medicinal fern of the genus Huperzia, and the secondary metabolite contained in it-Huperzine A has been proved to be a highly efficient, low-toxic, reversible and highly selective acetylcholinesterase inhibitors. At present, huperzine A preparations have been listed as the first choice drug for the treatment of senile dementia at home and abroad for clinical and research purposes. [0003] Due to the special molecular structure of huperzine A, its chemical synthesis steps are cumbersome, costly, and low in activity, so it does not have the value of large-scale commercial production. Although people have made beneficial expl...

AI Technical Summary

Problems solved by technology

[0003] Due to the special molecular structure of huperzine A itself, its chemical synthesis steps are cumbersome, costly, and low in activity, so it does not have the value of large-scale commercial production

Although people have made beneficial explorations on alternative resources such as the search for related plants of Melaleuca, spore propagation, artificial cultivation, tissue culture, and endophytic fungal fermentation, but with little success, it is basically in the initial stage of research and development

Therefore, the raw materials required for huperzine A preparations completely depend on the extraction of natural resources of Melaleuca, but the huperzine A content of the plants of Melaleuca is low, the growth is slow, the regeneration cycle is long, and the wild resources are very limited. The commercial recoverable volume is less than 200 tons

[0004] In recent years, with the acceleration of the aging process of the global population and the increasing number of people with senile dementia, the demand for huperzine A resources at home and abroad is increasing day by day. The wild resources are becoming increasingly scarce, far from meeting the current and future potential medical needs, and will make Melaleuca the biggest bottleneck in the huperzine A preparations industry chain

Images