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39results about How to "Short rooting time" patented technology

Full-haze cuttage seedling culture method for sweet viburnum

The invention discloses a full-haze cuttage seedling culture method for sweet viburnum, comprising the following steps of: (1) preparing a cutting matrix; (2) preparing a rooting reagent; (3) selecting cutting twigs and cutting; and (4) managing and transplanting the cutting twigs after cutting. By utilizing the full-haze cuttage seedling culture method for sweet viburnum disclosed by the invention, twigs with the depth of 50-80cm are taken for cutting; and as a result, fine characters of mother plants of sweet viburnum can be kept, the plant grows robustly and roots in a short time, the survival rate is high, and the plant grows rapidly and commercial seedlings can be produced in a short time. Compared with the existing cutting technology in the prior art, the method disclosed by the invention has the advantages that the cutting twigs are long and leafs are kept completely; the survival rate is not influenced by the depth and the size of the cutting twigs; the time for survival seedlings to grow to 100cm in height is saved by 70%-80% and the cost is saved by 75%-85%; and therefore, the method disclosed by the invention has high economic benefit and has important promotion and application values in the breeding production of sweet viburnum seedlings.
Owner:JIANGSU SANHE HORTICULTURAL

Method for constructing cutting orchard for crowned trunk embedding method of cunninghamia lanceolata

The invention relates to the technical field of plant propagation, and provides a method for constructing a cutting orchard for a crowned trunk embedding method of cunninghamia lanceolata. The methodcomprises the following steps: selecting a nursery ground, carrying out soil preparation and applying a base fertilizer, planting seedlings, shaping the scion cutting mother trees, constructing a scion cutting surface and carrying out hormone treatment, and constructing a photosynthesis surface and carrying out hormone treatment. The method solves the problems that the scion quality is poor, the rejuvenation degree is low, the yield is low, and the service life of the cutting orchard is short in other types of cunninghamia lanceolata cutting orchards.
Owner:福建林业职业技术学院 +3

Cuttage propagation method of rosa chinensis

The invention relates to a cuttage propagation method of rosa chinensis, and belongs to the technical field of the cultivation of rosa chinensis. The cuttage propagation method of the rosa chinensis, which is provided by the invention, comprises the following steps of 1), soaking flower mud into a naphthylacetic acid liquor; 2), taking the middle-lower part of a branch of the rosa chinensis to carry out trimming; 3), spraying carbendazol onto a cutting trimmed and obtained in the step 2); 4), inserting the cutting obtained in the step 3) into the flower mud, and burying the flower mud containing the cutting into a disinfected cuttage seedbed to carry out culture for 4 to 6 weeks, so as to obtain a cuttage rooting seedling of the rosa chinensis; 5), transplanting the cuttage rooting seedling of the rosa chinensis obtained in the step 4). The rosa chinensis obtained by the cuttage propagation method provided by the invention is short in rooting time, high in survival ratio, low in labor consumption and low in cost, and can be used for realizing the high-efficiency large-scale production of the rosa chinensis.
Owner:房丹

Method of raising ormosia seedlings by cutting

The invention discloses a method of raising ormosia seedlings by cutting. The method includes the steps of preparing cutting beds, to be specific, building the cutting beds in a greenhouse, providing each cutting bed with a plastic shed, mixing well fresh yellow soil, fine sand and nutrient soil proportionally to obtain cutting substrate, spreading the cutting substrate on the cutting beds, and sterilizing the cutting beds with potassium permanganate before cuttage; collecting cuttings, to be specific, using shoots which grow robust, are free of disease and pest damage and are already lignified, as cuttings, cutting flat the upper end of each stem, and cutting a 45-degree slope at the lower end of each stem; treating the cuttings, to be specific, soaking lower end cuts of the cuttings into composite rooting agent solution, and treating the shoots with sterilizing liquid for standby; performing cuttage, to be specific, inserting the cuttings into the cutting substrate, making sure the lower ends of the cuttings are in tight contact with the cutting substrate, and performing full irrigation; performing post-cuttage management, to be specific, keeping the interior of bow film at 20 DEG C to 28 DEG C under the humidity of 85% to 90% to make sure the cuttings and their leaves are glossy. The method has the advantages that rooting time is short, rooting rate is high, survival rate of seedlings is high and the method is suitable for popularization and application in production practice.
Owner:JIANGSU LVFENG LANDSCAPE ENG

Outdoor transplanting method of polygonatum sibiricum tissue culture seedlings

The invention relates to the field of polygonatum sibiricum cultivation, in particular to a transplanting method of polygonatum sibiricum tissue culture seedlings. According to the method, the survival rate of the tissue culture seedlings is increased by taking the polygonatum sibiricum tissue culture seedlings as materials and taking a series of measures such as selection and disinfection of a transplanting substrate, disinfection of a transplanting container and agentia and management of a seedling stage environment and water after transplanting. The provided polygonatum sibiricum transplanting technology is adopted for production, the method is simple and convenient, the medicinal value is increased, the survival rate of transplanting is high, the production method can be reduced, and the method is of great significance to the medicinal species protection.
Owner:CHINA THREE GORGES CORPORATION

Hollow heating seedling cultivating box and application thereof

The invention relates to a hollow heating seedling cultivating box and application thereof, and belongs to a method for cultivating seedlings of trees. A box of which the bottom internal diameter is 200cm*100cm and the height is 30cm is made of color-steel complex sheets, geothermal lines of which resistances are 33ohm / m are laid on the bottom of the box, and then the seedling cultivating box which is used for heating and controlling temperature is made. Inside the seedling cultivating box, three expanded polystyrene foam sheets of which the length, width and height are 199cm*3cm*10cm are erected on the two longitudinal sides and the middle part, cover plates of the seedling cultivating box in sizes of 99cm*3cm*10cm are paved on the three expanded polystyrene foam sheets in sequence, cutting slips are inserted in small semicircular grooves while the cover plates are paved, the roots of the cutting slips appropriately contact with a black mulching film, and the small grooves of the two adjacent foam sheets are butted with each other to hold the cutting slips until that the cutting slips are fully inserted in the whole seedling cultivating box. According to the method provided by the invention, the rooting rate of the seedling is more than 95%, the root quantity is large, the roots are uniformly distributed, second-grade lateral roots are easy to cultivate, and the roots are mature. Compared with conventional seedbeds, the rooting rate and the number of the plant roots are greatly improved; more importantly, 80% of matrix is saved, so that the seedling cost is greatly reduced.
Owner:JIANGSU UNIV OF SCI & TECH

Peony rooting culture method

The invention discloses a peony rooting culture method which comprises the following steps: stripping a few layers of bud scales from peony bulbil, washing with tap water for 30min and soaking and rinsing with carbendazim for 8-10min; sterilizing with 70% alcohol for 20-30s on an ultra-clean workbench; sterilizing again with 2% sodium hypochlorite or 0.1% mercury bichloride for 8min; washing with sterile water for 3-5 times; stripping the rest bud scales; cutting off some tissues of the base, and inoculating the rest part to a solid culture medium to obtain rootless seedlings; and transferring the rootless seedlings to a rooting culture medium to obtain peony seedlings. By adopting the method disclosed by the invention, the rooting rate and elongation of the seedlings can be effectively increased, so that the peony rooting time is shorter, and the survival rate is improved. The rooting time is shortened to 10 days, the maximum root length is 25.58mm, the rooting rate is over 70%, and the root number is 4.8; however, the contrasted rooting time is 20 days, the rooting rate is 10%, the maximum root length is 18.06, and the root number is 2.
Owner:HENAN AGRICULTURAL UNIVERSITY

Air-layering rooting and seedling-growing promoting method for mulberries

The invention discloses an air-layering rooting and seedling-growing promoting method for mulberries. The air-layering rooting and seedling-growing promoting method for mulberries includes the following steps of branch selecting and girdling, mud packaging, rooting cultivating and in-vitro seedling growing; rooting is induced by adding rooting powder into packaged mud and the moisturizing measure, and the survival seedling rate is increased. The air-layering rooting and seedling-growing promoting method for mulberries is easy to operate; mud is covered with films, moisture in mud is prevented from being evaporated, moist of matrixes is kept, and rooting is further facilitated; limitation of varieties, tree shapes and branches of mulberries do not exist, and almost all mulberries can root and grow seedlings with the method; cultivated roots of mulberry seedlings are good in development, particularly, main roots are sturdy, lateral roots are developed, young seedlings are robust, the transplanting survival rate can reach 80% or above, and the environmental adaptability is high; the rooting time is short, and the rooting quality is good; the speed and the quality of vegetative propagation of mulberries are greatly increased, and a guarantee is provided for good-seed breeding and popularization. In a word, the rooting quality of mulberries can be rapidly, efficiently and greatly improved with the method, the seedling survival rate and the transplanting survival rate of vegetative propagation of mulberries are increased.
Owner:广西壮族自治区蚕业技术推广总站

Rootless tissue culture method of lycoris radiata and lycoris radiata cultivating method

The invention belongs to the field of tissue culture, and relates to a rootless tissue culture method of lycoris radiata and a lycoris radiata cultivating method. The rootless tissue culture method oflycoris radiata, provided by the invention comprises the steps of performing induction on bulb blocks in induction culture mediums so as to obtain adventitious buds, wherein the induction culture mediums use MS as a base, also comprise 0.5-3.0mg / L of N-(phenylmethyl)-9H-purin-6-amine, 0.5-1.5mg / L of alpha-naphthaleneacetic acid, 30g / L of cane sugar and / or white sugar and 6-8g / L of agar; and culturing the adventitious buds in propagation culture mediums so as to obtain rootless tissue culture seedlings, wherein the propagation culture mediums use MS as a base and also comprise 3.0-5.0mg / L of N-(phenylmethyl)-9H-purin-6-amine, 0.5-1.5mg / L of alpha-naphthaleneacetic acid, 0-3mg / L of forchlorfenuron, 30g / L of cane sugar and / or white sugar, 0-1mg / L of thidiazuron and 7-8g / L of agar, and numberof times of propagation culture is 1-5. Through the adoption of the rootless tissue culture method of lycoris radiata provided by the invention, the survival rate is high.
Owner:SHANGHAI ACAD OF AGRI SCI

Matrix for cutting propagation of cyclocarya paliurus and processing method thereof

The invention discloses a matrix for the cutting propagation of cyclocarya paliurus and a processing method thereof. The matrix is composed of pennisetum purpureum, senecio scandens, garden humus soil, cattle manure crumbs, and river sand. The matrix can be applied to cutting propagation of cyclocarya paliurus and has the advantages of short rooting time and high rooting rate.
Owner:龙胜泓文生态农林有限公司 +1

Method for promoting rooting of alfalfa tissue culture seedlings

The invention discloses a method for promoting rooting of alfalfa tissue culture seedlings. The method comprises the following steps: A, preparing a solid rooting culture medium (1) in a hollow cylindrical shape; B, inoculating the upper end surface of the solid rooting culture medium (1) with alfalfa seedlings obtained by tissue culturing, and enabling the roots of the alfalfa seedlings to grow downwards in a semi-hanging manner along the inner wall of the solid rooting culture medium (1) in the hollow cylindrical shape; and C, sealing by a sealing membrane after inoculating, performing rooting culture in a tissue culturing chamber, and performing seedling hardening and transplanting 1-2 weeks later when the roots of the alfalfa seedlings grow to over 5cm. According to the method for promoting rooting of alfalfa tissue culture seedlings, the alfalfa tissue culture seedlings are subjected to semi-hanging rooting culture through the solid culturing medium with a specific structure for the first time, and an efficient alfalfa rooting culture system is established by selecting appropriate rooting culture medium and rooting agent. The problems that the alfalfa tissue culture seedlings are slow in rooting, fewer in side roots, not developed in root system, low in transplanting survival rate and the like can be solved.
Owner:INST OF ANIMAL SCI OF CHINESE ACAD OF AGRI SCI

Non-tube rootage method for camellia oleifem tissue culture seedling

The invention discloses a non-tube rootage method for a camellia oleifem tissue culture seedling. The method comprises the steps of inoculating a micro-branch which has 4-5 leaves, a slight red and brighten top leaf, a green stalk and height being 2-4cm and is obtained by performing subculture proliferation on a camellia oleifem seedling onto a root primordium induction culture medium for culturing till the base part generates a white root primordium, wherein each litre of the root primordium induction culture medium contains NAA of 3.0-5.0mg, and the rest root primordium induction culture medium is 1 / 2HB culture medium; taking the micro-branch with the white root primordium growing on the base part, washing the culture medium of the base part without roots and shoots, dipping with GGR (green plant growth regulator) of 200-600mg / L after filtering, instantly cutting onto a cutting medium, watering enough root fixed water, ensuring sufficient water and fertilizer supply during the growth period, and growing the micro-branch to a rooted seedling, wherein the cutting medium is formed by uniformly mixing 2-6 parts of yellow subsoil and 1-2 parts of plant ash. The method adopts non-tube rootage and breaks through the traditional mode in which the root system is induced in a tissue culture bottle, rootage and domestication are organically combined, direct rootage of the camellia oleifem tissue culture seedling outside a tissue culture bottle is realized, and the method has the advantages of short rootage time, well developed root system, absorption function and high transplantation survival rate.
Owner:JIANGXI ACAD OF FORESTRY

Hydroponics cutting rooting method for clematis florida

InactiveCN108719031AImprove cutting propagation efficiencyUniform nutritional statusCultivating equipmentsHorticulture methodsClematisGreenhouse
The invention discloses a hydroponics cutting rooting method for clematis florida. The hydroponics cutting rooting method includes selecting appropriate clematis florida cutting strips and placing theclematis florida cutting strips in rooting culture solution; culturing the clematis florida cutting strips under mist spray greenhouse conditions for 6 weeks; transferring clematis florida branches into hole tray substrates after a plurality of new root systems with the lengths of 2-3 cm grow on the clematis florida branches, and carrying out seedling growing management. The rooting culture solution is nutrient solution with auxin hormone. The hydroponics cutting rooting method which is a hydroponics method has the advantages that the clematis florida cutting strips can quickly root by the aid of the hydroponics cutting rooting method, the hydroponics cutting rooting method is high in rooting quantity, the root systems are plump and sturdy, the cutting seedling rate of the clematis florida can be increased to a great extent, key technical difficult problems in the aspect of clematis florida cutting propagation can be solved by the aid of the hydroponics cutting rooting method, and a foundation can be laid for quickly industrially propagating the clematis florida.
Owner:INST OF BOTANY JIANGSU PROVINCE & CHINESE ACADEMY OF SCI

Method for multilayer breeding of nursery stocks by adopting heatable temperature-controllable box

The invention relates to a method for multilayer breeding of nursery stocks by adopting a heatable temperature-controllable box, and belongs to a cultivation method for plant seedlings. A color steel compound plate is made into a box with the bottom inner diameter being 200*100 cm and the height being 30 cm, and a soil heating line with the resistance being 33 ohm / m is laid at the bottom of the box, so that the heatable temperature-controllable seedling breeding box is formed. The seedling breeding box is put on a seedling breeding shelf and filled with a seedling breeding matrix with the thickness of 15 cm, carbendazim liquid diluted by 2,000 times is used for watering the seedling breeding box thoroughly, cut cuttings are treated and inserted into the matrix, the temperature in the box is kept at 27-29 DEG C, the seedling breeding box is thoroughly watered by the carbendazim liquid diluted by 2,000 times every 8-10 days, and the matrix in the box is kept wet. The seedling breeding time is 30-40 days and seedlings bred in the temperature-controllable box are obtained. According to the method, the rooting rate of the bred seedlings reaches more than 92.0%, a large amount of root systems are uniformly distributed, secondary lateral roots grow out, and root development is relatively mature. Compared with planar seedling breeding, multilayer seedling breeding increases the number of seedlings bred in unit area and the method is suitable for factory breeding of the nursery stocks.
Owner:JIANGSU UNIV OF SCI & TECH +1

Cyclocarya paliurus cutting propagation substrate and processing method thereof

The invention discloses a cyclocarya paliurus cutting propagation substrate and a processing method thereof. The substrate is prepared with banana leaf sheath, star anise, humus garden soil, crushed cattle manure, and river sand. When the cyclocarya paliurus cutting propagation substrate provided by the invention is used for propagating cyclocarya paliurus, cyclocarya paliurus rooting time is short, and cyclocarya paliurus rooting rate is high.
Owner:龙胜泓文生态农林有限公司 +1

Method for raising seedlings by cutting for hevea brasiliensis in field

The invention relates to the technical field of plant propagation, and in particular relates to a method for raising seedlings by cutting for hevea brasiliensis in a field. The method includes the steps of establishment of a seedling raising bed, preparation of a seedling raising substrate and disinfection, preparation of cuttings and treatment of the cuttings, the cutting, management after the cutting and bagging transplanting. When the method for raising the seedlings by the cutting for the hevea brasiliensis in the field provided by the invention is used, a cutting rooting rate is significantly improved, an average cutting rooting rate achieves 80%, a root system of the cutting seedlings is well developed, and has more and developed lateral roots and hard texture, and a transplanting survival rate achieves 98%; according to the method for raising the seedlings by the cutting for the hevea brasiliensis provided by the invention, after the cutting is performed, artificial watering isnot needed, other equipment is not needed for heating, shading and light-filling treatment, so that the production costs are greatly reduced; and the method closely combines a cutting orchard with a cutting area, thereby greatly improving land utilization efficiency.
Owner:YUNNAN INST OF TROPICAL CROPS

A kind of heating hollow nursery box and its application

The invention relates to a heated hollow seedling cultivation box and an application thereof, belonging to a method for cultivating forest seedlings. A box with a bottom inner diameter of 200cm×100cm and a height of 30cm is made of color steel composite board, and a geothermal wire with a resistance of 33 ohms / m is laid on the bottom of the box to form a heated and temperature-controlled seedling box. Three expandable polystyrene foam boards with a length, width and height of 199cm×3cm×10cm are erected on both sides and in the middle of the seedling box, and the cover plates of the seedling box of 99cm×3cm×10cm are laid on them in turn, and the sides are paved. Insert the cuttings in the small semicircular groove, the base of the cuttings should be in contact with the black mulch film, and the two adjacent small grooves of foam boards are connected to each other to clamp the cuttings until the entire seedling box is filled. The method of the invention has a seedling rooting rate of more than 95%, a large number of root systems and even distribution, secondary lateral roots, and relatively mature root development. Compared with conventional seedbeds, the rooting rate and the number of roots per plant are greatly improved, and more importantly, more than 80% of the substrate is saved, which greatly reduces the cost of seedling cultivation.
Owner:JIANGSU UNIV OF SCI & TECH

Method for inducing rapid propagation of cluster bud through stem fragment of dendrobium officinale

The invention discloses a method for inducing rapid propagation of a cluster bud through a stem fragment of dendrobium officinale. The method comprises the following steps: step one, multiplication ofa stem fragment explant: taking a strong and high-quality plant of the annual dendrobium officinale, cutting leaves by using a sharp scalpel, washing by using running water, brushing the stem fragment by using a soft hair toothbrush, slightly stripping a white skin of the stem fragment, and using the stem fragment as the explant; soaking for 30 seconds by using 70-75% of ethyl alcohol, and washing for 3-4 times by using sterile water; processing for 10 minutes by using 10 times of bleaching water, and washing for 3-4 times by using the sterile water; and soaking by using 0.1% of mercuric chloride solution and shaking for 10-15 minutes, using the excellent individual stem fragment of the dendrobium officinale as the explant, and establishing a set of more complete stem fragment induced cluster bud tissue rapid-cultivation and propagation system. A stem fragment clone technology has the characteristics of early sprouting, short rooting time, and a rapid propagation speed, is not limitedby geographical environment and weather conditions, is not limited by seasons of seed collection, and capable of realizing annual clone production.
Owner:翁源县天下泽雨农业科技有限公司

Special low-cost soil improvable scindapsus aureus fertilizer

The invention discloses a special low-cost soil improvable scindapsus aureus fertilizer which comprises the following raw materials in parts by weight: 85-95 parts of a macroelement inorganic fertilizer, 0.1-0.2 part of calcium carbonate micro powder, 1-2 parts of humic acid magnesium, 0.1-0.2 part of humic acid zinc, 0.1-0.4 part of a nitro humic acid urea complexing agent, 1-2 parts of humic acid copper, 55-65 parts of an organic fertilizer, 0.001-0.002 part of bacillus subtilis, 0.01-0.02 part of bifidobacterium, 0.001-0.002 part of yeast and 2-8 parts of composite starch. The composite starch is prepared by using the following processes: pregelatinizing starch, uniformly mixing with water, adding sodium acrylate, acrylamide and a dispersing agent, uniformly mixing, adding calcined kaolin, N,N-methylene bisacrylamide and potassium peroxodisulfate, performing water bath stirring, drying, and crushing, thereby obtaining the composite starch.
Owner:肥西凤林家庭农场

A kind of hydroponic rooting method of cutting seedlings of medicinal industrial hemp

The invention discloses a hydroponic rooting method for medicinal industrial hemp cutting seedlings. A special hydroponic device including upper and lower nested water tanks, submersible pumps and foam boards is used. Tap water, sterilized water or distilled water is used as the hydroponic fluid. The submersible pump must be in working condition during the rooting process of cuttings; Before the flowering of industrial hemp, the semi-lignified or lignified 8-15cm main branch or the top leaf ear of the side branch is used as a cutting, inserted into the planting hole on the foam board, and the lower part of the cutting is immersed in the hydroponic solution; Cultivation environment conditions: air temperature is 25-35°C, water temperature is 20-25°C, the light is natural light, and the cutting seedlings are irradiated by natural astigmatism during the day; the leaves are sprayed with hydroponic liquid 4-6 times during the day every day to maintain the humidity of the leaves, and the present invention does not use chemical The roots of cutting seedlings can grow to more than 10cm in 12 to 22 days, and the rooting rate of cuttings can reach 100%. The hydroponic liquid does not need to be replaced midway, and the cost is low, and it is green and environmentally friendly.
Owner:云南省农业科学院经济作物研究所

Cultivation method of non-toxic seedlings of Paulownia albiflora

The invention discloses a non-toxic paulownia fortunei seedling culture method which includes the steps: 1) taking paulownia fortunei stem segments with axillary buds as explants and performing primary culture after disinfection to form primary seedlings; 2) performing virus-free culture for the obtained seedlings to obtain virus-free materials; 3) alternately placing the obtained virus-free materials into a high-concentration plant hormone subculture medium and a low-concentration plant hormone subculture medium for light culture to obtain rank seedlings; 4) dividing the obtained rank seedlings and then performing rooting culture to obtain rooting seedlings. The method is simple to operate, high in virus-free, propagation and rooting efficiency and high in transplanting survival rate, the obtained rooting seedlings have developed root systems and short fine hair (<1mm), and labor and time are saved when the seedlings are cleaned.
Owner:GUANGXI INST OF BOTANY THE CHINESE ACAD OF SCI

A method for multi-layer breeding of seedlings using a heating incubator

The invention relates to a method for multi-layer breeding of seedlings by using a heating incubator, belonging to a method for cultivating plant seedlings. A box with a bottom inner diameter of 200cm×100cm and a height of 30cm is made of color steel composite board, and a geothermal wire with a resistance of 33 ohms / m is laid on the bottom of the box to form a heated and temperature-controlled seedling box. Put the seedling box on the seedling rack, fill the seedling box with a 15cm thick seedling substrate, and pour it with 2000 times of carbendazim solution, insert the cut cuttings into the substrate after treatment, and keep the temperature in the box at 27-29°C , every 8‑10 days, the seedling box is poured with a 2000-fold carbendazim solution once to keep the substrate in the box moist. The seedling time is 30-40 days, and the incubator seedlings are obtained. The method of the invention has a seedling rooting rate of more than 92.0%, a large number of root systems and even distribution, secondary lateral roots, and relatively mature root development. Compared with flat seedling raising, multi-layer seedling raising increases the number of seedlings per unit area, which is suitable for factory breeding of seedlings.
Owner:JIANGSU UNIV OF SCI & TECH +1

A kind of rooting method of Camellia oleifera tissue culture seedling outside the bottle

The invention discloses a non-tube rootage method for a camellia oleifem tissue culture seedling. The method comprises the steps of inoculating a micro-branch which has 4-5 leaves, a slight red and brighten top leaf, a green stalk and height being 2-4cm and is obtained by performing subculture proliferation on a camellia oleifem seedling onto a root primordium induction culture medium for culturing till the base part generates a white root primordium, wherein each litre of the root primordium induction culture medium contains NAA of 3.0-5.0mg, and the rest root primordium induction culture medium is 1 / 2HB culture medium; taking the micro-branch with the white root primordium growing on the base part, washing the culture medium of the base part without roots and shoots, dipping with GGR (green plant growth regulator) of 200-600mg / L after filtering, instantly cutting onto a cutting medium, watering enough root fixed water, ensuring sufficient water and fertilizer supply during the growth period, and growing the micro-branch to a rooted seedling, wherein the cutting medium is formed by uniformly mixing 2-6 parts of yellow subsoil and 1-2 parts of plant ash. The method adopts non-tube rootage and breaks through the traditional mode in which the root system is induced in a tissue culture bottle, rootage and domestication are organically combined, direct rootage of the camellia oleifem tissue culture seedling outside a tissue culture bottle is realized, and the method has the advantages of short rootage time, well developed root system, absorption function and high transplantation survival rate.
Owner:JIANGXI ACAD OF FORESTRY

Method for rapidly inducing root primordia of blueberry tissue culture seedlings

ActiveCN112369330APromote the survival effect of transplantingGenerate efficientlyAgriculture gas emission reductionHorticulture methodsAlcoholPrimordium
The invention discloses a method for rapidly inducing blueberry tissue culture seedlings to generate root primordia. The method comprises the steps that adventitious buds obtained through blueberry tissue culture serve as materials, a placement mode of one layer of cloth and one layer of seedlings is adopted, the adventitious buds are placed on cloth with a root primordium inducer soaked, light-proof heat preservation and moisture preservation induction is carried out for a period of time, then the adventitious buds are pruned into stem segments, cuttage is carried out in a rooting medium, moisture preservation and heat preservation culture is carried out, the root primordia start to appear after 5 days, the inductivity of the root primordia can reach 95% after 15 days, and the rooting rate reaches 100% after 30 days. Compared with test-tube rooting, the method has the advantages that the steps of cleaning and transplanting hardened seedling are removed, the phenomenon that a large amount of roots are damaged in the cleaning and transplanting process of the test-tube rooted seedlings can be avoided, meanwhile, a rooting inducer is easily dissolved in water, NaOH or ethyl alcohol isnot needed for dissolution promotion, the pH value does not need to be adjusted, and therefore the damage of the NaOH or the ethyl alcohol to the tissue culture seedlings can be avoided, meanwhile, the time and the cost are saved; and the method is high in rooting rate and high in transplanting survival rate, and the problems of long rooting time and low transplanting survival rate of existing blueberry tissue culture seedlings are solved.
Owner:INST OF BOTANY JIANGSU PROVINCE & CHINESE ACADEMY OF SCI

Method for culturing Tulipa gesneriana L.

The invention relates to a method for quickly culturing Tulipa gesneriana L.. The method comprises the following steps of seedball processing, refrigeration processing, seedball disinfection, soil preparation for fertilization, sowing in a greenhouse, seedling stage management, flower forcing, flower bud harvesting, later-stage management and the like. According to the method, culturing time can be effectively shortened, a germination rate is improved, and root taking time is shortened. During plantation, a biological organic fertilizer can be adopted to effectively improve soil fertility, sandy nutrient soil and nutrient solution can be adopted to provide enough nutrients and growth environment for the growth of the Tulipa gesneriana L.. Greenhouse plantation and cultivation can be adopted to quicken Tulipa gesneriana L. to come into the market, which is slightly affected by seasons so as to be favorable for enlarging Tulipa gesneriana L. growth environment. Meanwhile, during plantation, illumination intensity can be effectively controlled and regulated, so that the chlorophyll content and the flower percentage of Tulipa gesneriana L. leaves can be increased, the color of the Tulipa gesneriana L. is vivid, the size of a corolla is proper, a flower season is prolonged, and therefore, the method has a good practical application and market promotion value.
Owner:郭欢欢

A rootless tissue culture method for Lycoris and a cultivation method for Lycoris

The invention belongs to the field of tissue culture, and relates to a rootless tissue culture method of Lycoris and a cultivation method of Lycoris. The invention provides a method for rootless tissue culture of Lycoris: inducing bulbs in an induction medium to obtain adventitious buds; the induction medium is based on MS, and further includes: 0.5-3.0 mg / L 6-benzylaminopurine, 0.5 ~1.5mg / Lα-naphthaleneacetic acid, 30g / L sucrose and / or white sugar and 6~8g / L agar; culture adventitious buds in proliferation medium to obtain rootless tissue culture shoots; proliferation medium is based on MS, and also Including: 3.0-5.0mg / L 6-benzylaminopurine, 0.5-1.5mg / L α-naphthaleneacetic acid, 0-3mg / L forchlorfenuron, 30g / L sucrose and / or white sugar, 0-1mg / L thidiazuron and 7-8g / L agar; the number of proliferation culture is 1-5 times. The rootless tissue culture method for lycoris provided by the invention has a high survival rate.
Owner:SHANGHAI ACAD OF AGRI SCI
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