A kind of rooting method of Camellia oleifera tissue culture seedling outside the bottle

Abstract

The invention discloses a non-tube rootage method for a camellia oleifem tissue culture seedling. The method comprises the steps of inoculating a micro-branch which has 4-5 leaves, a slight red and brighten top leaf, a green stalk and height being 2-4cm and is obtained by performing subculture proliferation on a camellia oleifem seedling onto a root primordium induction culture medium for culturing till the base part generates a white root primordium, wherein each litre of the root primordium induction culture medium contains NAA of 3.0-5.0mg, and the rest root primordium induction culture medium is 1 / 2HB culture medium; taking the micro-branch with the white root primordium growing on the base part, washing the culture medium of the base part without roots and shoots, dipping with GGR (green plant growth regulator) of 200-600mg / L after filtering, instantly cutting onto a cutting medium, watering enough root fixed water, ensuring sufficient water and fertilizer supply during the growth period, and growing the micro-branch to a rooted seedling, wherein the cutting medium is formed by uniformly mixing 2-6 parts of yellow subsoil and 1-2 parts of plant ash. The method adopts non-tube rootage and breaks through the traditional mode in which the root system is induced in a tissue culture bottle, rootage and domestication are organically combined, direct rootage of the camellia oleifem tissue culture seedling outside a tissue culture bottle is realized, and the method has the advantages of short rootage time, well developed root system, absorption function and high transplantation survival rate.

Description

Technical field: [0001] The invention belongs to the field of plant tissue culture, and in particular relates to a method for rooting out of a bottle of camellia oleifera tissue culture seedlings. Background technique: [0002] Camellia oleifem (Camellia oleifem) is a unique woody oil tree species in my country. The camellia oil produced by Camellia oleifera contains more than 90% unsaturated fatty acids, which is easily absorbed and digested by the human body, and has a good effect on reducing serum cholesterol and preventing hyperlipidemia and cardiovascular diseases; The tea dry and tea husks after oil extraction are also raw materials for extracting tea saponin, xylitol, producing high-protein feed and high-grade activated carbon and other comprehensive utilization products. The whole plant of camellia oleifera is a treasure, with high economic value, wide application, strong adaptability, and great potential for comprehensive development and utilization. It is known as "...

AI Technical Summary

Problems solved by technology

The seedlings cultivated by this method have poor root development, no root hairs, low degree of lignification, weak meristematic ability, slow growth rate, small stem cortex cells, underdeveloped leaf cuticles, and long-term incandescent light. Low content, weak photosynthetic ability, poor transpiration resistance and adaptability directly affect the survival of tissue cultured seedlings transplanted; and in the patented propagation method, the clustered buds cultivated once are respectively intercepted into aseptic seedlings containing one tender bud for the second time Subculture, through this method, only 1-2 axillary buds will be produced after the subculture of single shoots. As the number of subcultures increases, the degree of lignification of the buds is higher, and the stems become brown. Difficult to root, very low proliferation rate

Adopting MS as the subculture of Camellia oleifera through experimental research, although clustered buds can take place, the multiplication coefficient is more than 5, but the effective seedling (high 2-3cm) quantity that clustered buds produces is seldom, also affected the Camellia oleifera group. The process of large-scale production of seedlings

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