1803results about How to "Shorten the breeding cycle" patented technology

The invention discloses a method for cultivating a low-cadmium-accumulation indica rice variety. The method includes the steps of cloning part of the CDS sequence of OsNramp5 of an indica rice acceptor material; using a CRISPR / Cas9 system to select a target sequence according to an exon sequence; building pCRISPR / Cas9 recombinant vectors; leading the pCRISPR / Cas9 recombinant vectors into rice calluses to obtain transgene seedlings; screening transgene positive plants; obtaining mutant plants; and conducting seed propagation of the mutant plants, and separating afunction mutants containing no transgenic ingredients from plant progenies. By means of the method, the cadmium absorption major gene OsNramp5 of indica rice is directionally knocked out through the CRISPR / Cas9 technology, and the indica rice material containing no transgenic ingredients, having no significantly variant comprehensive agronomic characters and achieving a low cadmium content in rice is directionally cultivated. The method has the advantages that targeting is efficient, the breeding period is short, cost is low and practicality is high.

The invention belongs to the technical field of plant seedling culture, and discloses a seedling growing method for oil expression peony seeds. The method comprises measures of collection and processing of the oil expression peony seeds, seed storage, seedbed preparation, sowing and seedling raising, management after transplantation, and the like. Compared with the prior art, the method has the advantages that the seed processing time is short, the seed processing method is simple, the emergence of seedlings is tidy, the emergence rate is high, the seedling survival rate is high, the seedling raising cost is low, no season influence of transplantation after seedling survival exists and the like.

The invention discloses a vector sgRNA CRISPR / Cas9 relevant with character of rice reddish brown glume, vector construction and application, for overcoming the defect that rice with reddish brown glume can be acquired only on the condition of mutation of two alleles OsCHi. The invention provides an sgRNA segment which comprises a gRNA of which specificity is targeted to the fifth exon of cinnamylalcohol dehydrogenase(CAD) gene of rice; the sgRNA refers to T1, T2 and T3 specifically. The invention further provides a CRISPR / Cas9 gene editing vector comprising the sgRNA and a construction methodof the vector. The vector can edit cinnamyl alcohol dehydrogenase(CAD) gene of rice. Besides, application of the segments and the vector in acquiring rice having characters of reddish brown glume andstem earing, and primer sequence for detecting CRISPR / Cas9-CAD gene reconstruction are also provided. According to the invention, conventional breeding cost can be reduced, mechanization level of hybrid rice seed production can be improved, and seeding cycle can be shortened.

The invention discloses a seedling cultivating method for cutting hard woods of acer palmatum. The method comprises the following steps: (1) selecting an appropriate cutting time; (2) preparing a cutting substrate; (3) preparing rooting agent; (4) cutting slips processing and cutting; and (4) managing and transplanting after cutting. The seedling cultivating method for cutting the hard woods of the acer palmatum, disclosed by the invention, has the characteristics as follows: the cutting survival rate reaches more than 80%; compared with the cost of acer palmatum softwood cuttage, the cost of hard wood cuttage is reduced by 30-50%; the cost of hard wood cuttage is only about 20% of the cost of a tissue culturing method; and compared with a seedling cultivating period of the acer palmatum graftage, the seedling cultivating period of the hard wood cuttage is reduced by 2-3 years. The method disclosed by the invention has the advantages of simplicity, convenience and easiness in operation, low cost, simple technical requirements, easiness for promotion and application as well as important application and promotion values in acer palmatum seedling breeding production.

The invention belongs to the technical field of fruit trees and forestry cultivation, and discloses a method for promoting the lateral rooting of a annual carya illinoensis seed seedling, the root of a sprouting carya illinoensis seed seedling is mechanically cut, and a pair of scissors is utilized to cut the part below the fibrous root; and the seedling which is subjected to mechanical root cutting is transplanted into a nutrition pot, melon seed-shaped pebbles are laid on the bottom of the nutrition pot for 5 centimeters, and a nylon net is cushioned on the melon seed-shaped pebbles. A matrix is put in a container, the seedling is transplanted in a facility which is cold-proof and sun-proof for one month, a furrow which has the width of 13 centimeters and the depth of 20 centimeters is defined in a seedbed of a land, and the row spacing of the furrow is 30 centimeters. A layer of non-woven fabric is laid at the bottom of the furrow, a chemical rooting-resistant agent is coated on the non-woven fabric, and the nutrition pots which are planted with the seedlings are put on the non-woven fabric according to the planting distance of 20 centimeters, and are fixed finally. The water and fertilizer management period is normally carried out. The method can be used for effectively promoting the lateral rooting of the carya illinoensis seed seedling.

The invention discloses a Chinese medicinal herb additive for meat chickens and application. The Chinese medicinal herb additive comprises heterophylly falsestarwort root, nutgrass galingale rhizome, amur corktree bark, coptis root, medicated leaven, sanguisorba, hawthorn, Indian Buead, officinal magnolia bark, largehead atractylodes rhizome, tangerine peel, malt, indigowoad root, swordlike atractylodes rhizome, indigowoad leaf, cyrtomium fortune, chatoyancy and calamus; and mixture of the compositions is added with calcium bicarbonate, phytase, mulitivitamin for birds and lysine. The Chinese medicinal herb additive effectively promotes absorption of nutrient, can resist bacteria and virus, strengthen immunity of the meat chickens, effectively prevents bird flu, has obvious curative effect on diseases having respiratory tract symptoms as the major symptom, and has inhibiting function on familiar newcastle disease, colon bacillus, Pasteurella, enteritis, diarrhea, feather pecking, hair shedding, anus pecking, coccidiosis and the like. The additive cannot restrict the growth and disease resistance of the meat chickens, the meat quality of the meat chickens fed by the additive is obviously better than that of the meat chickens fed by the prior feed additive, the average daily gain of the meat chickens is improved by 23 to 28 percent than that of the meat chickens fed by the prior feed additive, the cultivation cycle is obviously shortened, and no harmful substance is deposited.

The invention discloses a lilium regale WRKY transcription factor gene LrWRKY1. The lilium regale WRKY transcription factor gene LrWRKY1 has a nucleotide sequence shown as SEQ ID NO: 1, and codes a protein with a nucleotide sequence shown as SEQ ID NO: 2. According to the lilium regale Wilson WRKY transcription factor gene LrWRKY1, functional genomics related technical research verifies that the LrWRKY1 gene has the function of increasing the fungus resistance of plants; when the antifungal LrWRKY1 gene is constructed to a plant expression vector and is transferred to tobaccos for overexpression, transgenic tobacco plants have strong in-vitro antifungal activities, and experimental results show that the LrWRKY1 overexpressed transgenic tobaccos have obvious inhibiting effects on the growth of four fungi including botryosphaeria, sclerotinite, botrytis cinerea and fusarium oxysporum.

The invention discloses a centralization indoor constant temperature circulation aquaculture system which includes two aquaculture ponds arranged side by side. A water inlet and a water outlet are arranged in each aquaculture pond. An aerator and a swell machine are arranged in each aquaculture pond. The centralization indoor constant temperature circulation aquaculture system is characterized in that the two aquaculture ponds are made of thermal insulation materials. A sewage treatment system is arranged between the two aquaculture ponds. A suction sewage device is arranged at the end of water outputting of each aquaculture pond. The suction sewage device is connected with the sewage treatment system through a sewage pipe. A water outlet of the sewage treatment system communicates with each aquaculture pond. The centralization indoor constant temperature circulation aquaculture system has the advantage that circulation utilization of water for aquaculture is achieved so that the quality of aquaculture aquatic products is improved and pollution of sewage discharges to environment is reduced.

The invention discloses bacillus pumilus, a probiotics preparation and a preparation method and application thereof. The Bacillus pumilus LV149 is preserved in China center for type culture collection (CCTCC) in Nov. 23th, 2011, and the preservation number is CCTCC NO: M 2011411. The bacillus pumilus LV149 has strong extracellular protease, lipase and amylase activities, has wide rejection capability to vibrio and has no hemolytic activity. The Bacillus pumilus LV149 serves as fermenting bacterial strains and is performed with solid fermentation, drying and smashing so as to prepare bacillus pumilus probiotics preparation which uses Bacillus pumilus LV149 as the active ingredients. The bacillus pumilus probiotics preparation can be added into prawn feeds for feeding prawns, so that growth of prawn intestinal pathogenic vibrio can be restrained, prawn vibriosis can be reduced, simultaneously prawn growth is promoted, fish bait coefficient is reduced, quality of commodity is improved, culture cycle is shortened, accordingly culture risk and culture cost are reduced, biological safety is high and the bacillus pumilus, the probiotics preparation and the preparation method and application thereof have wide application prospect in aquaculture.

The invention discloses a fast tissue culture reproducing method of actinidia eriantha, belonging to the technical field of plant tissue culture. The method comprises the following steps: (1) tissue culture medium preparation; (2) explant selection and sterilization; (3) inducing culture; (4) proliferation culture; (5) rooting culture; (6) tissue culture plantlet acclimatization and transplant, and the like. The fast tissue culture reproducing method has high culture medium pertinence and good applicability, the inductivity of an initial sprout of an explant reaches more than 80 percent, the proliferation rate of a successive sprout reaches 2-7 times, the plantlet height can reach 2-5 centimeters for 20-30 days, the quality of tissue culture plantlets is enhanced, the rooting rate reachesmore than 95 percent, and the transplant survival rate reaches more than 95 percent. The invention can be popularized and applied to fruit tree plantlet enterprises.

The invention discloses an efficient environmental-friendly black carp extrusion compound feed and a production method thereof. The method is characterized by comprising the following steps: mixing large amounts of other raw materials such as fish meal, extruded-soybean, soybean meal, rapeseed meal, cottonseed meal, wheat and rice bran and then coarsely crushing a mixture; adding spray-dried animal blood cells, larvae powder, monocalcium phosphate, choline chloride and salt for evenly mixing, carrying out superfine crushing; adding vitamin premis, mineral premix, rheum emodin, L-carnitine and allicin for evenly mixing; and carrying out steam tempering, extrusion, granulation, drying, spraying, cooling and the like on the mixed materials to obtain the product. The black carp extrusion compound feed is simple to prepare and has comprehensive and balanced nutrition, the digestibility of the black carp on feed nutrient substances can be improved, the feed coefficient is reduced, and the nitrogen and phosphorus emission is lowered. The feed has good palatability, excellent stability in water, and less feed wastes and water pollution. The feed can increase the immunity, antioxidation and anti-stress capabilities of the black carp, and promotes the growth of the black carp.

The invention discloses a management system and method of intelligent livestock breeding. An intelligent fodder feeding module in the system is used for detecting real-time edible condition of materials of livestock in real time and supplying fodders of predicted fodder put volume according to livestock individual growth data, the number of feeders is reduced, the breeding cost is controlled, thescientific nature, standardization and degree of automation of the livestock breeding are improved; an intelligent environment control module collects environmental parameters in a breeding environment, and the livestock breeding environmental parameters are adjusted in the most suitable growth range; a disease monitoring module monitors the disease condition of the livestock to reduce the epidemic situation infection probability of the livestock; a growing prediction module predicts the growth tendency and best slaughter time of the livestock according to the individual growth data; a qualitytraceability module records, stores and inquires the breeding information in the livestock breeding process; and the intelligent management of the livestock breeding is realized together, and the purposes that the number of the feeders is reduced, the breeding period is shortened, the breeding cost is controlled and the livestock breeding is scientific and standard are achieved.

The invention belongs to the fields of a gene sequence and an obtaining method of the gene sequence, and particularly relates to an obtaining method of an SNP (single nucleotide polymorphism) site and a CAPS (cleaved amplified polymorphic sequence) mark interlocked with a citrullus lanatus fruit bitter taste gene Bt (bitterness). The SNP site and the CAPS mark interlocked with the citrullus lanatus fruit bitter taste have nucleotide base sequences in SEQ ID NO:1-2 in a sequence table. The citrullus lanatus fruit bitter taste gene is primarily positioned by screening a bitter taste single plant in an RILs colony and combining with the high-density genetic map information of the citrullus lanatus; the candidate SNP site interlocked with the character is combined with separate colony verification analysis and natural colony verification analysis; the mark tightly interlocked with a target character is obtained; the SNP site and the CAPS mark can be applied to improvement of citrullus lanatus variety and molecular assisted breeding; a technical support is provided for molecular breeding of the citrullus lanatus quality; and meanwhile, the traditional gene positioning time is greatly shortened.

The invention belongs to the technical field of cotton molecular breeding, and discloses SNP molecular markers associated with fiber strength of upland cotton as well as detection of the SNP molecular marker and application of the SNP molecular marker. The SNP molecular marker takes a cotton stable RIL group as a material, and is obtained through a genome re-sequencing method. The SNP markers are utilized to perform molecular marker assisted breeding, so that the breeding period can be greatly shortened, cotton fiber strength is enhanced, and the breeding efficiency is improved.

The invention provides a method for sprouting and rapidly seedling a maolai seed, comprising the following steps of: seed collection; seed meat and oil removal; acid etching treatment; hormone treatment; sand mixing and cold storage; sowing; sprout transplantation; and seedling management. By using the method for sprouting and rapidly seedling the maolai seed, provided by the invention, the annual germination rate and planting percent of seeds can be remarkably increased, the seed culturing period can be shortened, and the production cost can be reduced.

The invention discloses an air layering breeding method for chaenomeles speciosa, and belongs to a breeding method for the chaenomeles speciosa. The air layering breeding method comprises steps as follows: step A, skin girdling; step B, wound wrapping; step C, shearing from a parent body; and step D, planting: a plastic membrane arranged on a mud ball in a covering manner is removed, the root of a branch sheared from the parent body is disinfected and plated together with the mud ball on a planting field, soil on the planting field is used for completely covering the mud ball at the root of the branch, and then root fixing water is poured. Under the condition that the shape of the chaenomeles speciosa plant is not destroyed, the suitable branch is selected directly for accomplishing seedling raising on a tree, the seedling raising period is shortened effectively, the branch which is to be sheared on the chaenomeles speciosa plant can be selected for seedling raising, the branch to be sheared can be used effectively, and waste is changed into valuable; and rooting is accomplished on a seed tree, so that the survival rate of seedlings after field planting is improved greatly and normally can be higher than 95%, and the rate of bred commercial seedlings can be higher than 90% when measures such as auxiliary support, container transformation and the like are combined.

The invention discloses an oil camellia bud seedling stock-grafting propagation method in winter and spring. The oil camellia bud seedling stock-grafting propagation method in winter and spring comprises processes such as stock cultivation, seedling growing bed preparation, seedling growing container preparation, grafting, grafted seedling planting, and seedling management. By heating a germination bed in winter and spring and utilizing the lifting-type seedling growing bed for growing seedlings, oil camellia bud seedling stock-grafting and grafted seedling cultivation are achieved in winter and spring, cultivation in an enclosed high-temperature and high-moisture environment is guaranteed for the initial stage of the oil camellia bud seedling stock-grafting, the survival rate reaches to 98.56%, average height in the same year reaches to 28.34 cm, and the ground diameter is 3.56 mm. Therefore, the survival rate of the grafted seedlings is increased, the grafted seedlings are capable of coming out of a nursery in the same year, the seedling cultivation circle is shortened, the using rate of cutting wood is increased, and the survival rate and seedling growing quality of oil camellia large-scale grafting are effectively improved.

The present invention relates to one fast improved walnut plant propagating process with low cost, fast seedling growth, high survival rate, high quantity, simple operation and high efficiency. The process includes the following steps: seeding walnut seed after germination stimulation and soaking in gibberellin liquid to seedling bed to cultivate tree stock; collecting scion from branch of mother plant and sealing with wax; grafting the scion with wedged lower end after being soaked with naphthylacetic acid solution to the tree stock; cutting the roots of the grafted seedling and treating roots with naphthylacetic acid solution; and planting the grafted seedling to the seedling bed while exposing the cut over ground and watering for rooting.

The invention provides a grafting and seedling-cultivating method for a Theaceae plant. A grafting and seedling-cultivating manner of the grafting and seedling-cultivating method is that seeds of the Theaceae plant are refrigerated; pre-germination is conducted in due time according to the shoot growing condition of a scion supply plant at every time, and the pre-germinated seeds are cultivated into root stocks in a seedling cultivating container; and a root stock 1.2 to 2.0mm in diameter is selected, and the upper end of the root stock is cut off at a distance of 3 to 5cm from the ground, so as to graft the root stock. The method provided by the invention is particularly suitable for the grafting and seedling cultivation of oil camellia and has the advantages that the grafting can be realized for multiple times within one year, so that the seedling cultivation cost is effectively reduced; through selecting and grafting appropriate root stock materials, the grafting survival rate is effectively increased; and through appropriately controlling conditions of pre-germination, seedling cultivation, and the like, the seedling cultivation period is effectively shortened.

The invention discloses a method for breeding a new species of lean meat type pigs-Shennong No. 1, which includes: A) the erhualian sow as female parent and the yorkshire boar as male parent are in cross breeding to obtain the yorkshire*erhualian cross bred sow; then, cross breeding female parent is in cross breeding with the danish landrace boar to obtain danish*( yorkshire*erhualian) ternary hybrid pigs; B) a fundamental selective breeding group of danish*( yorkshire*erhualian) ternary hybrid pig is formed and selected as zero-generation of Shennong No. 1; C) the boars and sows are mated to obtain piglets, good piglets are selected gradually for seed reservation; D) the good piglets are continuously cultivated until sexual maturity, the outstanding individual female and male pig are reserved as the next generation of swine respectively according to reproductive performance and meat quality as well as carcass performance and growth performance; E) the step C) and D) are repeatedly operated for 4-15 times. The main selective breeding indexes of the Shennong No. 1 pig are based on the reproductive performance and meat quality, the Shennong No. 1 pigs have the advantages of good meat quality, which is manifested in red meat color, good water holding capacity, high intramuscular fat, and good tenderness and the like. Referring to the reproductive performance, the number of the first-born Shennong No. 1 sow is more than ten.

The invention discloses a container nursery method for cinnamon. The method comprises the procedures of selection of nutrient cups, preparation of a nursery substrate, seed collection and treatment, cup planting of seedlings and cultivation management. The method comprises the following specific steps: preparing the prepared nursery substrate in the nutrient cups; treating collected cinnamon seeds; planting the seedlings into the cups; finally performing cultivation management to obtain cinnamon nursery stocks. The nursery substrate is prepared by mixing yellow soil, coco coir and perlite in the ratio of (1-6):1:1. According to the method, sowing seeds are saved, and the production cost is reduced; moreover, the nursery substrate has higher physical properties and is more suitable for the growth and development of the cinnamon, a brand new cultivation system is established for the cinnamon, the survival rate and growth amount of the cinnamon are increased greatly, and a good economic benefit is achieved; the cultivation of the nursery stocks in cinnamon containers is not influenced by soil property, land which is unsuitable for land cultivation can be utilized, the land resources are protected effectively, and better social and ecological benefits are achieved.

The invention provides a formula of a meat rabbit feed and a preparation method thereof. The feed contains a coarse feed, a plant feed and a Chinese herbal medicine additive. The formula of the meat rabbit feed comprises the following components: a coarse feeder including corn flour, tapioca flour, peanut meal, bean pulp, rice bran, bone meal and aginomoto; a plant feed including ryegrass, oat grass, timothy grass, radish leaves, sweet potato stem and carrot; a Chinese herbal medicine additive including plantain herb, dandelion, purslane, mother chrysanthemum, honeysuckle, folium isatidis and radix isatidis. The meat rabbit feed disclosed by the invention is wide in source, low in cost, abundant in nutrient, and free of antibiotics and hormones; the culture-cycle of the meat rabbit can be shortened, the immunity of the meat rabbit is enhanced, and the economic benefits of meat rabbit breeding are improved.

The invention relates to a feed preparation method, and especially relates to a preparation method of probiotic fermented feed. The invention comprises the following steps of: well mixing of raw materials, that is, uniformly stirring rice husk meal, wheat bran, and fish meal; well mixing inoculation, that is, diluting mother inoculum liquid with water, well mixing, adding into the well mixed raw materials to obtain a water content of the solid feed of 35-45%; granulation, that is, preparing the inoculated feed into granules or powder; sealed fermentation, that is, performing fermentation at 25 DEG C-35 DEG C in sealed environment. With the above technical scheme, the invention has the following beneficial effects: the investment is less; the method is simple and easy to master; the bacterial strain is easy to store; the purposes of disease prevention and disease treatment are reached; the survival rate is high; reproduction is promoted; offensive odor of excrement is eliminated; maggots and flies are removed; the environment is purified; the cost is reduced; the taste is improved; the culture period is shortened; and the yield increasing and income increasing effects are obvious.

The invention discloses an unmanned-monitoring underwater cultivation robot. The unmanned-monitoring underwater cultivation robot comprises an aircraft body, and further comprises a datum collecting module and a datum treatment module. The datum collecting module comprises a camera, a temperature and salinity sensor and five constant water quality device, and the datum treatment module comprises an image recognizing module and an aquatic product growing condition extraction module. According to the unmanned-monitoring underwater cultivation robot, the aquatic product growing condition is extracted based on the fusion algorithm of images and water quality parameters, and the recognizing accuracy rate is increased; operations such as the fact that a fish is caught to be measured are avoided,harm to the fish is reduced, whether a fish is sick or not is timely inspected, timely controlling and precautions are conducted, the cultivation period of fries can be shortened in a certain extent,timely controlling can be conducted on diseases of the fries, and thus the purpose that the income is increased is achieved.

The invention discloses an alfalfa breeding method using cold plasma processing. The alfalfa breeding method using the cold plasma processing disclosed by the invention comprises the following steps: (1) carrying out the cold plasma processing on alfalfa raw materials to be processed to obtain the materials subjected to the cold plasma processing; and (2) selecting alfalfas with the target property from the materials subjected to the cold plasma processing. The alfalfa breeding method using the cold plasma processing disclosed by the invention breaks through the potential dangers that the traditional breeding has a low level and is repeated, the homogenization is serious and the transgenosis breeding is carried out, and overcomes the disadvantage that the economic input of space breeding is great; the breeding period can be shortened; the alfalfa breeding method using the cold plasma processing has the advancement in the aspect of breeding methods and means.

The invention discloses a breeding method for improving disease prevention ability of doves. A traditional dove breeding method does not pay attention to the improvement of the disease prevention ability of the doves, and therefore survival rates, breeding rates and growing speed of the doves are influenced in different degrees. According to the breeding method for improving the disease prevention ability of the doves, the doves are bred by a normal method, and meanwhile, a microbial technology can be used for carrying out environmental improvement and biological control. The microbial technology and Chinese medicine annexing agent can be used for improving internal conditions of the doves and the disease resistance capacity of the doves. The method can be used for breeding the doves, chemical drugs are basically not used in the whole breeding process by observing 30 thousands of pairs of the doves and the doves grow healthily. Compared with the normal breeding method, dove babies need 25-28 days to grow up to fly out of the bars and 22-25 days are just needed to breed the doves when the breeding method for improving the disease prevention ability of the doves is used; when the normal breeding method is used, female doves are mated with male doves to give birth to 1.5 dove babies every month, and when the breeding method for improving the disease prevention ability of the doves is used, the female doves are mated with the male doves to give birth to 1.7-1.8 dove babies every month; when the normal breeding method is used, the qualification rate of the eggs laid by the female doves is 98%, and when the breeding method of improving the disease prevention ability of the doves is used, the qualification rate of the eggs laid by the female doves is 99%. Therefore, the breeding method of improving the disease prevention ability of the doves is capable of improving the disease prevention ability of the doves.

The invention discloses an intelligent management system of animal husbandry production. The intelligent management system comprises a main control computer. The intelligent management system also comprises one or more subsystems selected from an environmental control subsystem, a fodder delivery subsystem, a radio frequency identification (RFID) subsystem and a video monitoring subsystem; the main control computer is used for distributing parameter configuration information required by the subsystems to the subsystems; the environmental control subsystem is used for monitoring environmental parameter information of a livestock farm, and controlling and making the environmental parameter information of the livestock farm be in a set range; the fodder delivery subsystem is used for monitoring fodder eating conditions of livestock products and controlling the delivery of fodder usage; the RFID subsystem is used for marking the livestock products and recording related data information of the livestock products; and the video monitoring subsystem is used for performing video recording and image capture on the livestock farm. With the intelligent management system adopted, intelligent management of livestock breeding can be realized, and a breeding cycle can be shortened, and breeding cost can be controlled, animal husbandry production can be carried out scientifically and standardly.

The invention belongs to the technical field of molecular breeding of cotton and discloses an SNP molecular marker associated with fiber strength of upland cotton as well as detection and application thereof. The SNP molecular marker is obtained by taking stable RIL group of cotton as the material through a genome resequencing method. When the SNP markers are used for carrying out molecular marker-assisted breeding selection, the breeding period can be greatly shortened; the breeding efficiency of the cotton can be improved; the cotton fiber strength can be improved.

The invention relates to a technical method for high-efficient breeding of wheat, which comprises low-temperature vernalization, south breeding and generation adding, shuttle breeding, multi-point combined identification and the like. In particular, the technical method comprises the following steps of: treating the seeds obtained after the strong-winterness wheat blossoms for 15 to 20 days by hydrogen peroxide, washing the seeds by pure water, sprouting the seeds at the room temperature, putting the seedlings growing to 1 to 2 centimeters in an artificial weather room, performing the green-body vernalization of the seedlings for 45 and 50 days at a temperature of between 3 and 5 DEG C and a relative humidity of 70 percent and under a light intensity of 15001x; hardening the seedlings of the winter wheat undergoing the vernalization treatment at the normal temperature for 1 to 2 days, and transporting the seedlings to Yuanmou in Yunnan province; and preparing a rice seedling bed, watering and soaking the rice seedling bed, discharging visible water before transplanting, and transplanting the seedlings in mud at dusk. The disease resistance, stress resistance, adaptability and yieldstability selections of breeding materials are carried out by shuttle breeding and multi-point combined identification and the like. The technical method for breeding provides rich reserve of raw materials for the rapid improvement of the wheat variety, and also lays a sold foundation for demonstration and popularization of a new variety in large areas.

The invention discloses a low-carbon and environment-friendly fish-farming method. The method comprises the following steps: (1) performing cage culture in a pond and assembling a fish feces recovery system and a translation bottom sewage self-leaking system under a cage to recover fish feces, so that sewage and deposits at the bottom of the pond are discharged effectively, and pollution of fish feces and residual feeds on a water body is lowered; (2) arranging aeration equipment outside a cage culture zone to promote flow of the upper, lower and surface layers of the water body and increase dissolved oxygen of the water body; (3) planting aquatic plants in a floating bed and combining pond culture and wet land planting and culture outside the cage so as to realize coexistence of fishes and vegetables and biologically purify water; (4) performing scientific fish culture by adopting a scientific culture mode represented by seven words, namely, water, planting, feed, prevention, separation, mixing and labor. According to the low-carbon and environment-friendly fish-farming method, the current-stage pond fish culture facility is changed, engineering of innovative culture equipment is realized, scientific design and scientific layout are achieved, scientific combination of comprehensive technologies such as physical and biological purification and the like is performed, combination with an ecological and environment-friendly culture technology is realized, and integrated innovation is implemented.