288results about How to "Reduced germination rate" patented technology

The invention relates to a Paris polyphylla var. yunnanensis scale seedling culturing method. The method comprises the following steps of: selecting Paris polyphylla var. yunnanensis plants more than 10 years old and reserving seeds for planting; collecting mature seeds; washing the collected seeds to remove episperm; spreading and drying the seeds in a shade and ventilating place within 12 hours, wherein a temperature is maintained and an accumulation thickness is less than or equal to 6 cm; spreading the seeds uniformly and drying; filling the seeds into a ventilating container; storing the container in a shade environment with a suitable temperature, wherein the storage time is no more than 2 months; clearing impurities and residues in a seedling culturing field; fertilizing decayed farm manure and calcium superphosphate; plowing and raking soil; forming a furrow; spreading humus soil on the furrow surface; flatting the furrow surface; sowing in early June next year; taking the stored seeds, and after sterilizing, broadcasting the seeds uniformly according to the thickness of sowing; earthing after broadcasting; covering mantle on the furrow surface; irrigating sufficiently and performing seedling management. A method for drying the seeds of the Paris polyphylla var. yunnanensis in shade in an environment without direct sunlight is adopted, the dormancy of the seeds of the Paris polyphylla var. yunnanensis is broken, and the scale production of Paris polyphylla var. yunnanensis seedlings is realized, so that germchit guarantee is provided for large-area popularization and cultivation of the Paris polyphylla var. yunnanensis. The method has the characteristics of simple and easily-learned operation, high survival rate of germchit, low production cost, healthy and strong germchit, stable and reliable technology, high repeatability, and capacity of effectively solving the problem of scale seedling cultivation of the Paris polyphylla var. yunnanensis.

The invention belongs to the technical field of plant seedling culture, and discloses a seedling growing method for oil expression peony seeds. The method comprises measures of collection and processing of the oil expression peony seeds, seed storage, seedbed preparation, sowing and seedling raising, management after transplantation, and the like. Compared with the prior art, the method has the advantages that the seed processing time is short, the seed processing method is simple, the emergence of seedlings is tidy, the emergence rate is high, the seedling survival rate is high, the seedling raising cost is low, no season influence of transplantation after seedling survival exists and the like.

The invention discloses a pinellia ternata planting method. The pinellia ternata planting method comprises the steps as follows: (1) growing a propagation material; (2) cultivating; (3) carrying out field management; and (4) harvesting. A pinellia ternata tuber with a diameter of 0.7-1.0 cm is adopted as a pinellia ternata seed source in the step (1) of growing the propagation material; the step (2) of cultivating comprises the sub-steps of selecting land, unpacking and planting; and planting land with a pH value of 6.0-7.5 is selected in the sub-step of selecting land. According to the pinellia ternata planting method, the pinellia ternata tuber with the diameter of 0.7-1.0 cm is adopted as the pinellia ternata seed source, and the soil with the pH value of 6.0-7.5 is adopted as the planting land for planting and cultivating pinellia ternata, so that the emergence rate of the pinellia ternata can be guaranteed, the soil requirement is low, the cost is reduced, and the yield is increased. According to the statistics, after the pinellia ternata planting method is adopted, the dry weight of the yield per mu (1 mu is 1 / 6 of acre) of the planted pinellia ternata can reach 220-260 kg / mu and is increased by 46.7% relative to that of the yield per mu of the conventional planted pinellia ternata. Therefore, the pinellia ternata planting method is suitable for large-scale planting.

The invention also relates to a microorganism fertilizer. The composite microorganism preparation comprises the components based on the volume ratio: 5-15% of photosynthetic bacteria, 10-20% of bacillus subtilis, 15-35% of saccharomycete, 25-45% of thermophilic bacillus and 3-15% of actinomycete. The invention also relates to the application of the microorganism fertilizer in the aspects of improving the resistance of seeds, preventing plant diseases and insect pests, purifying the water quality of rice field and increasing the rice production, and a using method thereof. The invention also relates to the application of the microorganism fertilizer in the aspect of accelerating rice stubble rotten degree, and a using method thereof.

The invention provides a brown rice germinator and a dual respiration type germination technology, and relates to a brown rice germination production technology and the manufacturing of a germination device in the grain processing field. The invention causes the brown rice to uniformly obtain the water, temperature and fresh air needed by germination by utilizing the characteristics of large heat capacity, unshaped property, small viscosity and easy flow and the like of water and controlling the water temperature and carrying out the operation sequences of water-injection and water-draining respiration and pumping vacuum respiration to the brown rice in a container, and sequentially completes the procedures of cleaning, soaking, germination and inactivation and the like to the brown rice. Therefore, the invention solves the key technological problems in the industrialized production of large-scale germinated brown rice, such as a plurality of times of brown rice transfer and transmission, high production cost and low germination rate and the like, and compared with the optimized experimental method, the germination rate for producing the germinated brown rice can reach more than 95 percent.

The invention discloses a laser drilling device assisted by laser-induced impact waves. The laser drilling device comprises four modules, namely, a workpiece clamping device, a laser drilling device, a device capable of allowing a laser to induce the impact waves and a control system device, wherein the laser drilling device and the device capable of allowing the laser to induce the impact waves are arranged on the workpiece clamping device from top to bottom, and the control system device is connected with the laser drilling device and the device capable of allowing the laser to induce the impact waves through cables. According to the laser drilling device assisted by the laser-induced impact waves, the depth limit of laser drilling can be improved, the thickness of a recast layer can be lowered, the micro-crack producing rate can be lowered, the laser drilling efficiency can be improved, and the laser drilling device can be applied to laser drilling of medium plates and thick plates.

The invention relates to a culture method for Alcaligenes faecalis strain KZJ01 from oceans, characterized in that the Alcaligenes faecalis strain KZJ01 is obtained by the steps of enrichment culture, separation of strains, purification of strains, sieving of strains, authentication of strains and the like. The Alcaligenes faecalis strain KZJ01 obtained by the inventive culture method has the effect of inhibiting the growth of hypha of 9 plant pathogenic fungi and of inhibiting the germination of conidia of 2 plant pathogenic fungi therein.

The invention relates to a processing method allowing the seeds of Paris polyphylla var. yunnanensis to grow roots in four months and to grow leaves in eight months. The method comprises the following steps: step 1, in early September, selecting mature seed capsules of Paris polyphylla var. yunnanensis, spreading the seed capsules to low density and drying the seed capsules in the shade for 30 days; step 2, in early October, standing the Paris polyphylla var. yunnanensis seeds dried in the shade at a low temperature for 15 to 20 days to realize low temperature treatment; step 3, in mid-October, stripping capsule hulls of the Paris polyphylla var. yunnanensis seeds having undergone low temperature treatment, sowing the Paris polyphylla var. yunnanensis seeds with red coats in prepared seedling trays and carrying out seed germination; step 4, putting the seedling trays treated in the third step in a cold storage and carrying out low temperature treatment for 15 to 20 days; step 5, in mid-January, sowing the Paris polyphylla var. yunnanensis seeds having undergone low temperature treatment in the fourth step in a field with an altitude of 2800 meters; and step 6, carrying out manual weeding. In mid-April, a part of small seedlings of Paris polyphylla var. yunnanensis grow leaves; and in mid-June, more than 85% of the small seedlings of Paris polyphylla var. yunnanensis grow leaves. With the method of the invention, the germination rate and the seedling rate of the Paris polyphylla var. yunnanensis seeds reach more than 85%.

The invention relates to a sugarcane seedling culture method. The method comprises the steps of preparing sugarcane seedling culture matrix, laying the sugarcane seedling culture matrix, sowing seeds, and moving a sowing frame to a greenhouse provided with a sunshade net and peripherally covered with thin film to perform seedling management. According to the method, the matrix is rich in nutrition, the seedling culture operation is simple, the seedling culture cost is low, and the method can be popularized and used on a large scale. According to the sugarcane seedling culture method, the average germination rate of seeds reaches 98.4%, the average survival rate of temporary planting reaches 99.0%, and obtained sugarcane seedlings are robust and excellent.

The invention provides a wave-shaped covering flow collection cultivation technical mode of a wide-width water-seepage mulching film, belongs to the technical field of crop cultivation and aims at further improving light, heat and moisture resource use ratio in drought and cold areas and greatly increasing crop yield in unit area. The wave-shaped covering flow collection cultivation technical mode is characterized in that a plurality of rows are ridged in a farmland, ridge top grooves are furrowed on ridge peaks along the ridges, the groove bottoms of the ridge top grooves are higher than groove bottoms of ridge grooves arranged between every two ridges, the width of the furrowed farmland is 1300mm, the width of the mulching film is larger than 1600mm, wave-shaped covering is adopted, holes are punched on the mulching film at the positions of the ridge top grooves for seeds to be sowed, and a little amount of soil is covered on the mulching film. The number of the ridges is determined according to crop species, four rows are needed to be ridged when medium-density crops are planted, five rows are needed to be ridged when high-density crops are planted, and the mulching film is wide in width and water permeable. By adopting the wave-shaped covering flow collection cultivation technical mode, seedlings can be emerged completely, earth temperature is improved, soil moisture content is increased and soil maturity is good.

The invention discloses a pest control method for efficient cultivation of polygonum multiflorum, which comprises agricultural control, physical control, biological control and chemical control. The agricultural control comprises the following steps: in summer and autumn, after harvesting the last batch of crops, deep ploughing and drying upturned soil in time, exposing and drying the soil, wherein the thickness of deep ploughing is 25-30 cm, and the exposing and drying time is 30 days. By the method that in summer and winter, after harvesting the last batch of crops, the upturned soil is deeply ploughed and dried in time, and the soil is exposed and dried, wherein the thickness of deep ploughing is 25-30 cm, and the exposing and drying time is 30 days, germs invading polygonum multiflorum plants can be conveniently controlled; and thus the control efficiency is improved. The ploughing depth can reduce requirements to the ploughing tools and machines. In addition, the exposing and drying time is limited to be 30 days, which not only cannot enable the nutritional components in the soil to be lost, but also can improve the physical behaviors of the soil, reduce influence of the pest and disease damage to polygonum multiflorum, ensure the emergence rate of polygonum multiflorum, and improve the output of polygonum multiflorum.

The invention provides a method for substituting for flaveria bidentis by utilizing grain amaranth; in the method, the grain amaranth is adopted to carry out biological substitution to the flaveria bidentis; the method can lead the germination rate of the flaveria bidentis to be reduced by 80.43 percent, the average plant height is reduced by 77.30 percent, the average single plant branches are reduced by 98.96 percent, the average single plant leaves are reduced by 99.14 percent, the average single plant biomass liveweight is reduced by 92.54 percent, the average soluble protein content is reduced by 52.99 percent, and the method has remarkable control function to the foreign invasion of the flaveria bidentis; the grain amaranth is a common plant, the seed is easy to obtain, the planting method is simple, and the operation is easy; the grain amaranth is pasture with good quality, while controlling the population quantity of the flaveria bidentis, the grain amaranth has a certain economical value.

The invention discloses a high-frequency laser pulse method applied to dissimilar metal composite heat source welding. Through high-frequency pulse laser beam intervention, disturbance force in a molten pool can be increased, the Marangoni convection effect is enhanced, heat and mass transfer in the molten pool is accelerated, and uniform distribution of elements in the molten pool is achieved; aweld joint is shaped by directly or indirectly intervening a weld seam pool through a high-frequency pulse laser beam, weld seam grains can be refined, and a flat joint morphology which is symmetrically distributed along the center line of a weld seam is obtained; deformation maladjustment caused by difference of thermophysical performance parameters of dissimilar metal materials can be made up, so that the effect of intervening weldments in different time scales and space scales is achieved, and the reliability of dissimilar metal weld seam joints is improved; and the phenomenon of stress concentration induced by asymmetric distribution of pore and microcrack defects can be effectively prevented, and then the comprehensive mechanical property of the dissimilar metal weld seam joints is improved.

The invention relates to a rapid breeding method of lemon stocks. The method comprises the steps of stock seed pretreatment, seedling cultivating bed preparation, ridging and planting, double-film covering as well as transplanting of seedlings with embryos. By using the technical method provided by the invention, problems of low emergence rate, long production time and the like of stock seed seedling planting can be solved; meanwhile, the rapid breeding method of the lemon stocks provided by the invention has the advantages of simplicity, practicability, easiness in operation and short time consumption; and compared with the traditional seedling cultivating method, the rapid breeding method of the lemon stocks provided by the invention has the advantages as follows: the stock seedling cultivating period can be reduced by 30 days, the emergence rate and survival rate of the stocks are greatly increased and the emergence rate can be high up to 85%. The technical method provided by the invention can be also used for producing other citrus stocks.

The invention discloses a method and a matrix for breeding hybrid embryos of peach trees by the aid of test tubes on the basis of one-step seedling formation. The method includes acquiring sterile hybrid embryos, and to be more specific, disinfecting and treating seed embryos of seeds of the fruit trees to obtain samples of the sterile hybrid embryos; cultivating the sterile hybrid embryos in the test tubes filled with cultivation substrates to obtain embryo-germination plants which are test tube plants; acquiring plant seedlings outside the test tubes, to be more specific, transplanting the test tube plants into seedling growing substrates outside the test tubes when a plurality of lateral roots grow on radicals of the test tube plants and new shoots with the lengths longer than 10 cm grow on most embryos of the test tube plants, and cultivating the test tube plants to obtain plants outside the test tubes. The method and the matrix have the advantages that the embryo-germination test tube plants can be cultivated in the cultivation substrates in the test tubes, the plants which can normally grow in outdoor environments can be cultivated in the seedling growing substrates outside the test tubes, accordingly, the seeds of the fruit trees no longer need to be directly sowed, and the emergency rate of the seeds of the hybrid peach trees can be increased.

The invention relates to the field of plant stress resistance, and in particular relates to a corn transcription factor ZmWRKY44 and an application thereof. The corn transcription factor ZmWRKY44 has a nucleotide sequence as shown in SEQ ID No.1. According to the technical scheme, the expression condition of the adversity-tolerant gene of a transcription factor ZmWRKY44 arabidopsis plant under a salt stress condition is observed, the influence on the seed germination rate of the transcription factor ZmWRKY44 abrabidopsis plant caused by salt stress is relieved, and the plant growth is inhibited, so that a transgenic plant has a proper sensibility to the salt stress, and the transcription factor ZmWRKY44 can be used for enabling the plants to have a salt-tolerance property.

The invention relates to a culture medium for carpinus putoensis embryos, which comprises the following raw materials: a WPM salt, an MT vitamin, a BA (benzyl adenine) which is 0.001mg / l to 0.1mg / l, an inose which is 50mg / l to 150mf / l, a gelrite which occupies 0.2 percent to 0.4 percent of the total weight of the culture medium and a cane sugar which occupies 1.5 percent to 4.5 percent of the total weight of the culture medium. The pH value of the culture medium is 5.0 to 6.0. The process for tissue-culture and rapid reproduction by using the culture medium comprises the following four steps of: the collection and selection of explants, the cleaning and sterilization of seeds and the adoption of emryos, the inoculation and culture, the subculture and root induction and the culture of transplants. The invention can obtain carpinus putoensis regrowths in large volumes by the method of tissue culture and rapid reproduction, thereby saving the unique germplasm resource of the earth. The invention also discloses a method for an excellent culture medium and rapid reproduction of seedlings to the society and lays a foundation for further research of carpinus putoensis and exploration of a new biological engineering.

The invention relates to a method for breeding a saussurea involucrata plant. In the method, the lower hypocotyl stem section of the saussureae involucratae aseptic seedling after cutting off root and leaves is used as the explant, the explant is induced by using solid culture media with different concentrations of colchicine to generate a tetraploid saussureae involucratae plant, and the tetraploid saussureae involucratae is identified by using the tetraploid screening identification technology. The method provides an improvement method for optimal colchicine concentration, processing time and processing temperature for inducing generation of tetraploid, and morphology, chromosome counting and flow cytometry for identifying tetraploid. The method can be used for quickly breeding the autotetraploid saussureae involucratae plants; and the method shortens the breeding cycle, thereby effectively increasing the content of active ingredients, and overcomes the defect of variety degeneration of the saussureae involucratae in natural propagation and artificial planting.

The invention discloses a seed sprouting device for agricultural planting. A discharge port at the bottom end of a containing slot can be inserted into a groove; a fastening block push against the discharge port to fix the containing slot; a rotating motor can drive a rotating shaft to rotate forward, so that external threads at the bottom end of the rotating shaft are screwed out of a threaded hole; the rotating shaft is separated from the containing slot; the rotating motor drives the rotating shaft to stir plant seeds in the containing slot; furthermore, a blower can feed gas into a liquidstorage slot through an air conveying header pipe and a stirring rod; an oxygen content of liquid can be increased; a soakage sprouting effect of the plant seeds can be improved; the reduction of a sprouting rate of the plant seeds due to oxygen deficit can be avoided; with the adoption of a drying slot, the sprouted seeds can be dried; the two ends of a filter sieve plate in the drying slot can be driven by electric extension rods to reciprocate up and down; and the drying efficiency of the plant seeds can be improved.

The invention provides a seedling raising method of white gourd. The method comprises the following specific steps: preparing a seedling-raising substrate, accelerating germination of white gourd seeds, cultivating and raising seedlings. Through the seedling raising method of white gourd, the healthy growth of the seedlings is accelerated; the stress resistance of the seedlings is improved; the occurrence rate of pest and disease damage in the later period of the seedlings is reduced; the yield and the quality of the white gourd in the later period are ensured; the seedling raising method is simple and is easy to operate; the large-scale production is easy to implement; meanwhile, the planting cost is also reduced; the economic benefit is increased.

The invention discloses usage of an integrated soil sterilization and disinfestation device, which is characterized by including: starting a speed regulating motor after igniting fuel in a heating furnace; adding the soil not sterilized or disinfested into a sterilization and disinfestation chamber from a feed hopper; using flame in the heating furnace to externally heat a tubular duct; successively killing pathogens and nematodes in soil after interior temperature of the sterilization and disinfestation chamber exceeds 60 DEG C; and discharging the sterilized and disinfested soil from a discharge pipe and exhausting water vapor from an exhaust pipe. The pathogens, the nematodes and seeds of weeds in the soil can be successively killed by using the integrated soil sterilization and disinfestation device, the soil is applicable to cuttage and seeding of flowers, seedlings and vegetables, sprouting ratio of seeds is increased, germinating rate of the weeds is reduced, normal growth and development of the flowers, the seedlings and the vegetables are ensured, and the ecological environment can be protected from pollution. The usage of the integrated soil sterilization and disinfestation device is applicable to sterilization, disinfestation and drying of various types of soil.

The invention discloses a cutting propagation method for a glede tea raw material plant. The method comprises the following steps of: selecting semi-lignified branches and trimming into branches of 5-10 centimeters with 1-2 leaves; and soaking the trimmed branches with a GGR (Greenplant Growth Regulator) rooting powder formula, cutting into sandy loam developed by shale, ridging a high furrow, digging grooves of which the depths are 5-10 centimeters, arranging the branches at the intervals 5 centimeters, covering with earth in a way of leaving 2-3 centimeters of the branches above the ground and exposing leaves, always keeping 50-70 percent of shade and keeping the soil moisture at 60 percent. The cutting propagation survival rate of the glede tea raw material plant is increased remarkably, and can be over 90 percent.

The invention provides a novel tissue-culture seedling raising method for Gentiana rigescens Franch on the basis of the characteristics of low germination rate and low cutting propagation rate of aerospace breeding seeds of the Gentiana rigescens Franch. The novel tissue-culture seedling raising method mainly comprises the following steps: selection and disinfection of explants, induction of cluster buds, proliferation of the cluster buds and culture of rooted seedlings. The method provided by the invention is simple and practicable, has the advantages of short propagation period, high propagation rate, capability of effectively stabilizing the characters of a mother plant and the like, and is the most effective way for producing Gentiana rigescens Franch aerospace species with stable characters on a large scale.

The invention discloses a method for high throughput screening of microorganisms for preventing plant soil-borne fungal diseases, and belongs to the technical field of agricultural microorganisms. Themethod includes preparing the diseased oil with a high-concentration wheat root rot pathogenic bacteria culture and the soil, sowing germinated wheat seeds into the soil containing pathogenic bacteria to simulate the natural growth conditions and environment of plants and the pathogenic bacteria in the soil to the maximum extent. The method for screening for a pathogen-plant pathogenesis system of antagonistic microorganisms of the wheat bipolaris sp, the dense planting of the wheat reduces the space occupied by the screening, and the symptoms of the wheat root rot appear early, which reducesthe screening time, so that the high-throughput screening can be achieved. The screening efficiency of the microorganisms with better inhibition of pathogenic bacteria obtained in the early screeningis greatly improved, and a large number of microorganisms that cannot exert control effects in practical applications can be removed, so that the pace of microorganism screening, microorganism pesticide development and microorganism fertilizer research and development can be accelerated.

The invention provides a novel special liquid fertilizer for rice capable of comprehensively controlling plant diseases and insect pests, and a preparation method thereof. The novel special liquid fertilizer is prepared from the following components: 5 to 15 parts of urea-ammonium nitrate solution, 5 to 15 parts of ammonium polyphosphate solution, 5 to 10 parts of potash fertilizer, 0.1 to 2 parts of microelement, 20 to 50 parts of water, 1 to 10 parts of homemade plant pesticide extracting solution, 1 to 2 parts of soil conditioner, 1 to 5 parts of natural activity organic materials, and 1 to 5 parts of natural surfactant, wherein the homemade plant pesticide extracting solution is prepared from mixing trifolium pratense, folium artemisiae argyi, herba houttuyniae, semen persicae and black false hellebore in proportion, and extracting with water. The preparation method comprises the steps of adding the prepared urea-ammonium nitrate solution and the ammonium polyphosphate solution in proportion according to a formula into a mixing tank; then adding the other metered materials into the mixing tank; stirring, dissolving and filtering to prepare the liquid fertilizer. The liquid fertilizer provided by the invention is convenient to use and good in effect, and is a special multi-functional environment-friendly liquid fertilizer for the rice.

The invention discloses an orchid seed preserving and seedling raising method. The method comprises the steps: harvesting orchid capsules developed basically ripe and having no cracking, disinfecting, transferring seeds in the capsules into a sterilized centrifugal tube under an aseptic condition, covering with a centrifugal tube cover and sealing with a breathable membrane, and preserving under a condition of the temperature of 4-6 DEG C; and when seedling raising is needed, taking out the preserved seeds, placing to the centrifuge tube at room temperature to reach room temperature, making the seeds into a seed suspension with sterile water, taking the seed suspension and then evenly inoculating a one-step seedling formation culture medium with the seed suspension, and culturing into seedlings by one time. After the orchid seeds are stored for 5 years under the condition of the temperature of 4-6 DEG C, the orchid seeds still maintain a relatively high germination rate (75.6%); moreover, required facility conditions are simple, cheap, pollution-free and huge in preserving capacity; the seeds preserved by the method have no need for surface sterilization treatment, are subjected to aseptic sowing by using a non symbiotic germination technology, and can be cultured into seedlings at one time.

The invention provides a wheat mutation breeding method and aims to improve the chemical mutagenesis efficiency, generate a relative high point mutation frequency and have relative little chromosomal aberration. The wheat mutation breeding method disclosed by the invention adopts a 0.5% EMS (Ethylmethane Sulfonate)-phosphoric acid buffering solution to treat wheat pollen to induce andro gametes to have variation so as to obtain variant seeds; the 0.5% EMS-phosphoric acid buffering solution takes a phosphoric acid buffering solution as a solvent and a chemical mutagen ethylmethane sulfonate EMS is diluted to the volume ratio concentration of 0.5%; the mol concentration of the phosphoric acid buffering solution is 1 / 15mol / L; the dosage of the 0.5% EMS-phosphoric acid buffering solution is 25-50 microliters on each floret; and the wheat pollen is pollen which is about to be ripe or pollen grains which are primarily ripe.