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Cultivation method and use of Bacillus foecalis alkaligenes from sea

A technology of Alcaligenes faecalis and a culture method, which is applied in the field of culture of strains and can solve the problem of few microorganisms

Inactive Publication Date: 2009-07-15
HUAIHAI INST OF TECH
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, most of the microbial strains currently used for biological control come from land, and the screening of terrestrial microorganisms over the years has resulted in fewer and fewer chances of screening microorganisms that produce new disease-resistant mechanisms, so more and more people are turning their attention to The ocean, hoping to develop new anti-pathogenic active substances from the extremely diverse microorganisms in the marine environment, for the biological control of plant diseases

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  • Cultivation method and use of Bacillus foecalis alkaligenes from sea
  • Cultivation method and use of Bacillus foecalis alkaligenes from sea
  • Cultivation method and use of Bacillus foecalis alkaligenes from sea

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Embodiment 1

[0055] Example 1. A kind of cultivation method of Alcaligenes faecalis strain KZJ01 (Alcaligenes faecalisstrain KZJ01) from ocean, its specific isolation and cultivation steps are as follows:

[0056] (1) Enrichment culture: collect sea mud from the sea area of ​​Lianyungang, put 10 g of sea mud into a triangular flask containing 10 mL of sterile seawater, shake and shake well, take 5 mL of the suspension and add it to 50 mL of enrichment culture medium, 170r / min, cultured on a shaking table at 25°C for 48 hours;

[0057] (2) Isolation of bacterial cells: After 48 hours of culture on a shaking table, 1.0 mL of enrichment culture solution obtained from enrichment culture for 48 hours was taken with a pipette gun and added to a test tube containing 9.0 mL of sterile seawater to prepare 10 -1 The sample diluent; and then serially diluted by gradient dilution method, respectively prepared into 10 -2 、10 -3 、10 -4 、10 -5 、10 -6 、10 -7 sample diluent; then take 10 -5 、10 -...

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Abstract

The invention relates to a culture method for Alcaligenes faecalis strain KZJ01 from oceans, characterized in that the Alcaligenes faecalis strain KZJ01 is obtained by the steps of enrichment culture, separation of strains, purification of strains, sieving of strains, authentication of strains and the like. The Alcaligenes faecalis strain KZJ01 obtained by the inventive culture method has the effect of inhibiting the growth of hypha of 9 plant pathogenic fungi and of inhibiting the germination of conidia of 2 plant pathogenic fungi therein.

Description

technical field [0001] The invention relates to a method for cultivating strains, in particular to a method for cultivating Alcaligenes faecalis from the sea; the invention also relates to the application of Alcaligenes faecalis. Background technique [0002] At present, plant diseases mainly rely on the control of chemical fungicides. Due to excessive use, the environment and agricultural products are polluted, and some pathogenic bacteria have developed resistance to pesticides. The method of biological control makes up for the deficiency of chemical control, and has been paid more and more attention, and has become an important means of plant disease control. However, most of the microbial strains currently used for biological control come from land, and the screening of terrestrial microorganisms over the years has resulted in fewer and fewer chances of screening microorganisms that produce new disease-resistant mechanisms, so more and more people are turning their atten...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A01N63/02A01P3/00C12R1/05
Inventor 马桂珍暴增海夏振强刘云鹤王伟霞
Owner HUAIHAI INST OF TECH
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