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Orchid seed preserving and seedling raising method

A preservation method and seed technology, applied in the field of plant germplasm resource preservation, can solve the problems of flower industry loss, loss of flower germplasm resources, loss and the like, and achieve the effects of simple facility conditions, low preservation cost, and population restoration.

Active Publication Date: 2017-03-08
SOUTH CHINA BOTANICAL GARDEN CHINESE ACADEMY OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The loss of flower germplasm resources in my country has been very serious in history, and it is still losing now. If effective measures are not taken to protect my country's orchid germplasm resources, it will bring huge losses to my country's flower industry

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] Embodiment 1: preservation and seedling raising method of five lip orchid (Doritis pulcherrima) seeds

[0025] 1. Artificial pollination: when the orchid orchids bloomed in early August of that year, select robust plants. At 10:00 am during the day, use a sterilized toothpick as a tool to pick the active pollen clusters on the flowers that have been open for about two days. Confer it on the stigma of another flower that has been open for about two days, and pollinate 20 flowers in total. After pollination, remove the lip flaps of the female parent and bag them. After about 1 week, remove the bag and hang a label. The label has information such as the name of the parent, the name of the female parent, the date of hybridization, and the pollinator.

[0026] 2. Cultivate capsules: After successful artificial pollination, strengthen the fertilizer and water management of the female plant to ensure the normal development of artificially pollinated capsules until they mature....

Embodiment 2

[0031] Embodiment 2: Preservation and seedling raising method of floret Vanda coerulescens (Vanda coerulescens) seeds

[0032] 1. Artificial pollination: When the floret Vanda orchid blooms in early September of that year, select a robust plant. At 10:00 a.m. in the daytime, use a sterilized toothpick as a tool to pick the active pollen from the flower that has been open for about two days. Confer it on the stigma of another flower that has been open for about two days, and artificially pollinate 20 flowers in total. After pollination, remove the lip flaps of the female parent and bag them. After about 1 week, remove the bag and hang a label. The label has information such as the name of the parent, the name of the female parent, the date of hybridization, and the pollinator.

[0033] 2. Cultivate capsules: After successful artificial pollination, strengthen the fertilizer and water management of the female plant to ensure the normal development of artificially pollinated caps...

Embodiment 3

[0038] Embodiment 3: preservation and seedling raising method of Dendrobium officinale Kimura et Migo (Dendrobium officinale Kimura et Migo) seeds

[0039] 1. Artificial pollination: When Dendrobium officinale blooms in early March of that year, choose a robust plant. At 10:00 am in the daytime, use a sterilized toothpick as a tool to pick the active pollen pieces on the flowers that have been open for about two days. On the stigma of another flower that has been open for about two days, pollinating 20 flowers in total. After pollination, remove the lip flaps of the female parent and bag them. After about 1 week, remove the bag and hang a label. The label has information such as the name of the parent, the name of the female parent, the date of hybridization, and the pollinator.

[0040] 2. Cultivate capsules: After successful artificial pollination, strengthen the fertilizer and water management of the female plant to ensure the normal development of artificially pollinated c...

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Abstract

The invention discloses an orchid seed preserving and seedling raising method. The method comprises the steps: harvesting orchid capsules developed basically ripe and having no cracking, disinfecting, transferring seeds in the capsules into a sterilized centrifugal tube under an aseptic condition, covering with a centrifugal tube cover and sealing with a breathable membrane, and preserving under a condition of the temperature of 4-6 DEG C; and when seedling raising is needed, taking out the preserved seeds, placing to the centrifuge tube at room temperature to reach room temperature, making the seeds into a seed suspension with sterile water, taking the seed suspension and then evenly inoculating a one-step seedling formation culture medium with the seed suspension, and culturing into seedlings by one time. After the orchid seeds are stored for 5 years under the condition of the temperature of 4-6 DEG C, the orchid seeds still maintain a relatively high germination rate (75.6%); moreover, required facility conditions are simple, cheap, pollution-free and huge in preserving capacity; the seeds preserved by the method have no need for surface sterilization treatment, are subjected to aseptic sowing by using a non symbiotic germination technology, and can be cultured into seedlings at one time.

Description

technical field [0001] The invention belongs to the technical field of plant germplasm resource preservation, and in particular relates to a method for preserving and raising seedlings of orchid seeds. Background technique [0002] Orchidaceae, commonly known as orchids, has a cultivation history of nearly 2,000 years in my country and is an important cultivated plant in horticultural flowers. Most of the orchids are rare and endangered plants. All wild orchids in the world are included in the protection scope of the Convention on International Trade in Endangered Species of Wild Fauna and Flora, accounting for more than 90% of the plants that should be protected by the convention. "Flagship" taxon. The loss of flower germplasm resources in my country has been very serious in history, and it is still losing now. If effective measures are not taken to protect my country's orchid germplasm resources, it will bring huge losses to my country's flower industry. Contents of the...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/008
Inventor 吴坤林曾宋君郑枫张建霞段俊
Owner SOUTH CHINA BOTANICAL GARDEN CHINESE ACADEMY OF SCI
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