Wheat mutation breeding method
A technique of mutagenesis breeding and wheat, which is applied in the fields of botany equipment and methods, applications, plant gene improvement, etc., can solve the problems of weak competitive ability of mutant cells, low mutation rate, difficulty in growing into new individuals, etc., and achieve self-bred pure Effects of faster combination, less physiological damage, and shortened breeding period
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[0015] Take Jinmai No. 47 wheat variety as an example. Since its approval in 1995, Jinmai No. 47 wheat variety has been dominant in drylands due to its high and stable yield and strong drought resistance. However, the plant of Jinmai No. 47 is taller, the stem elasticity is poor, and it is prone to lodging in wet years or fertile dry land. The goal of our experiment is to improve the plant height and stem elasticity of Jinmai 47 on the basis of retaining the excellent characteristics.
[0016] Trials began in 2009. Wheat variety Jinmai 47 was sowed and planted at the test site, and EMS was used for pollen mutagenesis at the early flowering stage. Mutagenesis works around 9am or 3pm.
[0017] The specific steps of mutagenesis are as follows:
[0018] (1) Solvent preparation: prepare 1 / 15mol / L disodium hydrogen phosphate (Na 2 HPO 4 ) solution and 1 / 15mol / L sodium dihydrogen phosphate (NaH 2 PO 4 ) solution 100 mL each; take 1 / 15mol / L Na 2 HPO 4 Solution 61.1 mL, 1 / 15m...
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