Product
Patsnap Eureka
For R&D, Patsnap Eureka makes reading and utilizing patents & technical documents easy.
Patsnap Eureka AIR
Designed for self-driven R&D workflows. Generate viable solutions, solve complex R&D challenges, empower your innovation with AI.
Patsnap Eureka Materials
Designed for material experts only. Revolutionize your material R&D, from search, analyze, to developing new materials.
Feature
TechResearch
Generate reliable direction feasibility study reports for your R&D in just a few steps.
TechSeek
Discover and master advanced knowledge NOW. Basics, ideas, possibilities, all at once.
TechMind
As an expert in R&D Theories, TechMind can generates customized viable solutions instantly.
TechRisk
Analyze your overall solution with one click, know your potential R&D risks in advance.
TechMonitor
Get weekly tech updates, stay abreast of the latest tech innovations and key insights.
Patents
Literature
Hiro is an intelligent assistant for R&D personnel, combined with Patent DNA, to facilitate innovative research.
370 results about "Mutation rate" patented technology
Efficacy Topic
Property
Owner
Technical Advancement
Application Domain
Technology Topic
Technology Field Word
Patent Country/Region
Patent Type
Patent Status
Application Year
Inventor
In genetics, the mutation rate is the frequency of new mutations in a single gene or organism over time. Mutation rates are not constant and are not limited to a single type of mutation, therefore there are many different types of mutations. Mutation rates are given for specific classes of mutations. Point mutations are a class of mutations which are small or large scale insertions or deletions. There are also Missense and Nonsense mutations, which are variations of point mutations. The rate of these types of substitutions can be further subdivided into a mutation spectrum which describes the influence of the genetic context on the mutation rate.
Antibodies and methods for generating genetically altered antibodies with enhanced effector function
InactiveUS20050054048A1Increase variabilityBetter pharmacokinetic profileAnimal cellsSugar derivativesGenetic diversityMonoclonal antibody
Dominant negative alleles of human mismatch repair genes can be used to generate hypermutable cells and organisms. By introducing these genes into cells and transgenic animals, new cell lines and animal varieties with novel and useful properties can be prepared more efficiently than by relying on the natural rate of mutation. These methods are useful for generating genetic diversity within immunoglobulin genes directed against an antigen of interest to produce altered antibodies with enhanced biochemical activity. Moreover, these methods are useful for generating antibody-producing cells with increased level of antibody production. The invention also provides methods for increasing the effector function of monoclonal antibodies and monoclonal antibodies with increased effector function.
Owner:EISAI INC
Cognitive radio engine based on genetic algorithms in a network
ActiveUS20060009209A1Enable spontaneous inspiration and creativityNetwork traffic/resource managementGenetic modelsTransmitted powerGenetic algorithm
A genetic algorithm (GA) approach is used to adapt a wireless radio to a changing environment. A cognitive radio engine implements three algorithms; a wireless channel genetic algorithm (WCGA), a cognitive system monitor (CSM) and a wireless system genetic algorithm (WSGA). A chaotic search with controllable boundaries allows the cognitive radio engine to seek out and discover unique solutions efficiently. By being able to control the search space by limiting the number of generations, crossover rates, mutation rates, fitness evaluations, etc., the cognitive system can ensure legal and regulatory compliance as well as efficient searches. The versatility of the cognitive process can be applied to any adaptive radio. The cognitive system defines the radio chromosome, where each gene represents a radio parameter such as transmit power, frequency, modulation, etc. The adaptation process of the WSGA is performed on the chromosomes to develop new values for each gene, which is then used to adapt the radio settings.
Owner:VIRGINIA TECH INTPROP INC
Cognitive radio engine based on genetic algorithms in a network
ActiveUS7289972B2Enable spontaneous inspiration and creativityNetwork traffic/resource managementGenetic modelsAlgorithmTransmitted power
A genetic algorithm (GA) approach is used to adapt a wireless radio to a changing environment. A cognitive radio engine implements three algorithms; a wireless channel genetic algorithm (WCGA), a cognitive system monitor (CSM) and a wireless system genetic algorithm (WSGA). A chaotic search with controllable boundaries allows the cognitive radio engine to seek out and discover unique solutions efficiently. By being able to control the search space by limiting the number of generations, crossover rates, mutation rates, fitness evaluations, etc., the cognitive system can ensure legal and regulatory compliance as well as efficient searches. The versatility of the cognitive process can be applied to any adaptive radio. The cognitive system defines the radio chromosome, where each gene represents a radio parameter such as transmit power, frequency, modulation, etc. The adaptation process of the WSGA is performed on the chromosomes to develop new values for each gene, which is then used to adapt the radio settings.
Owner:VIRGINIA TECH INTPROP INC
CRISPR/Cas9 system-containing targeted knockout vector and adenovirus and applications thereof
InactiveCN104004778AHigh knockout rateGenetic material ingredientsViruses/bacteriophagesBiotechnologyEnzyme digestion
The invention discloses a CRISPR / Cas9 system-containing targeted knockout vector and adenovirus and applications thereof. The targeted knockout vector is prepared through the following steps: after a pX330 U6-Chimeric_BB-CBh-hSpCas9 plasmid is subjected to enzyme digestion and filling-in by using EcoRI and SacII, connecting the pX330 U6-Chimeric_BB-CBh-hSpCas9 plasmid with a pAdTrack-CMV plasmid subjected to enzyme digestion and filling-in by using BstXI; after the obtained product is linearized by using BbsI, connecting the obtained product to a specific target sequence of a desired gene; and after the obtained object is linearized by using PmeI and dephosphorylated by using CIAP alkaline phosphatase, recombining the obtained product with a pAdEasy-1 plasmid. The targeted knockout vector can mutate gene sequences in target sequence areas, and the mutation rate is high, up to 30.6-45.8%, therefore, the targeted knockout vector can be used for gene site-directed mutation, and lays a foundation for gene therapy.
Owner:重庆高圣生物医药有限责任公司
Double-label joint sequence for detection of tumor mutation and detection method
ActiveCN106086162AEasy to distinguishIncrease sample sizeMicrobiological testing/measurementLibrary creationMutation detectionBioinformatics
The invention discloses a double-label joint sequence for detection of tumor mutation, and is characterized in that the joint sequence is synthesized by a joint primer P5 and a joint primer P7, wherein the joint primer P5 has the sequence shown in SEQ ID NO:1, and the joint primer P7 has the sequence shown in SEQ ID NO:2. The invention also provides a database building method and a sequencing method. With use of a double-label joint, the tumor mutation rate of 1*10<-5> can be accurately detected, the tumor mutation detection sensitivity is effectively improved, and with combination of the flux of high-flux sequencing, one-time sequencing can detect multiple mutation loci of multiple genes.
Owner:AMOY DIAGNOSTICS CO LTD
Quality of service aware scheduling for composite web service workflows
A method of assigning web service requests to service providers includes searching for an optimal assignment from all possible assignments using a genetic algorithm (GA) that represents possible assignments as chromosomes, and converging towards an assignment of web service request to service providers that maximizes overall business value for all workflows to the service providers. An adaptive mutation scheme is used to introduce mutation into populations of chromosomes. The mutation scheme includes a mutation rate that increases when chromosomes under evaluation fail to improve its workload against the metric over a certain number of generations.
Owner:IBM CORP
Site-directed mutation method for genomes of saccharomyces cerevisiae
InactiveCN105624187AEfficient mutationRapid implementation of mutationsFungiMicroorganism based processesMicroorganismRepair site
The invention relates to the field of microorganisms, in particular to a site-directed mutation method for genomes of saccharomyces cerevisiae. To-be-repaired single-base mutation sites on the genomes of the saccharomyces cerevisiae can be repaired by the aid of CRISPR / Cas9 technologies. Effects can be realized near the to-be-repaired sites by Cas9 proteins under the guidance actions of guide RNA (ribonucleic acid) if the mutation sites are positioned in PAM sequences (NGG) or front 11bp of the PAM sequences, and the genomes of the saccharomyces cerevisiae can be subjected to double-strand break at cut positions, so that donor DNA (deoxyribonucleic acid) can be efficiently recombined. Compared with existing results, the site-directed mutation method has the advantages that the sites of the genomes of the saccharomyces cerevisiae can be efficiently and quickly mutated, and screening markers do not need to be integrated for the genomes of the saccharomyces cerevisiae.
Owner:TIANJIN UNIV
Primer, probe and kit for detecting EGFR and/or K-ras genetic mutation
ActiveCN105624309AEasy to optimizeMeet the requirements of rapid detectionMicrobiological testing/measurementDNA/RNA fragmentationK-ras GenesFluorescence
The invention discloses a primer and probe for detecting a human epidermal growth factor receptor (EGFR) gene and / or a K-ras gene, a kit containing the primer and the probe and a device for detecting genetic mutation on the basis of a digital PCR platform.The method for detecting the genetic mutation by means of the primer and the probe comprises the steps that the prime and the probe are provided; DNA of a sample to be detected is extracted; a fluorescent PCR reaction system capable of amplifying a mutant gene sequence is prepared; a target probe and an internal reference probe are utilized to be hybridized with amplified products respectively, and fluorescent signals of corresponding fluorescent groups are detected; existence of the genetic mutation is judged and / or the mutation rate is calculated according to the strength and proportion of the fluorescent signals of the target probe and the internal reference probe.According to the method for detecting the genetic mutation, the needed primers and probes are small in number, the optimization procedure is simple, related mutation of EGFR and / or K-ras gene can be qualitatively or quantitatively detected, and the detection sensitivity is high; a DNA sample with low initial amount can also be detected stably.
Owner:SHENZHEN HUADA GENE INST
Method for reducing CRISPR/Cas9 mediated embryo gene editing missing rate
InactiveCN107557394ATargeted editing efficiency is highLow rate of off-target mutationsStable introduction of DNAFermentationCompound aEmbryo
The invention relates to a method for reducing CRISPR / Cas9 mediated embryo gene editing missing rate. The method comprises the following steps: compounding a sgRNA sequence of a targeting gene and constructing a sgRNA expression plasmid carrying T7 promoter; in vitro transcribing Cas9mRNA and sgRNA; and importing intracytoplasmic sperm into unfertilized MII ovum by adopting intracytoplasmic sperminjection (ICSI) and meanwhile importing Cas9mRNA and sgRNA. According to the method provided by the invention, the targeting editing efficiency is high and the missing mutation rate is low.
Owner:NANJING DRUM TOWER HOSPITAL
Induction of viral mutation by incorporation of miscoding ribonucleoside analogs into viral RNA
InactiveUS20050187180A1High mutation rateReduced viabilityOrganic active ingredientsSsRNA viruses positive-senseRibonucleosideViral replication
Owner:UNIV OF WASHINGTON
CRISPR (clustered regularly interspaced short palindromic repeat)/Cas9 Recombinant lentiviral vector containing gRNA sequence specifically targeting CCR5 and application thereof
ActiveCN107312798AAvoid infectionInfection efficiency dropsOrganic active ingredientsGenetic material ingredientsRandom mutationLentivirus
The invention discloses a CRISPR (clustered regularly interspaced short palindromic repeat) / Cas9 recombinant lentiviral vector containing gRNA sequence specifically targeting CCR5 and application thereof. A lentivirus of the CRISPR / Cas9 recombinant lentiviral vector containing gRNA sequence specifically targeting CCR5 gene Delta 32 region is constructed that the lentivirus can introduce cells into a CRISPR / Cas9 system specific to CCR5, double-chain breakage occurs to a specific site of CCR5 gene, a random mutation is introduced to a breakage site after repairing by means of nonhomogeneous recombinant terminal binding, and the mutation rate reaches 90% and above. As gRNA is a nonhomogeneous region of CCR5 and CCR2, detection shows that the missing efficiency of the two gRNAs is lower than 0.2%. Cells modified via the recombinant lentivirus have significantly decreased efficiency of HIV (human immunodeficiency virus) infection. The system is quick to construct, simple and low in price, and is applicable to gene therapy of acquired immune deficiency syndrome.
Owner:WUHAN UNIV
Probe and sequence combination for simultaneous detection of various mutation types
InactiveCN106480205ARealize multiple comprehensive detectionAccurate detectionMicrobiological testing/measurementDNA/RNA fragmentationHydrothoraxTherapeutic effect
The invention discloses a probe and a sequence combination for simultaneous detection of various mutation types and particularly provides a kit, a method, a gene chip, a probe and a sequence combination which are high in sensitivity and specificity and used for simultaneous detection of point mutation, short segment insertion and deletion, copy number variation and fusion genes of 57 tumor driver genes listed in a detection list. The invention further discloses a sequence combination and probe associated gene chip and kit and a method for simultaneous detection of various mutation types. By detection of body fluid including blood, hydrothorax, abdominal dropsy and the like and tumor frozen tissues or paraffin sections, comprehensive tumor driver gene mutation information can be acquired, sensitivity and accuracy in tumor gene detection are improved, and accordingly clinicians can be assisted in making of individualized medication schemes to achieve best accurate treatment effects.
Owner:BEIJING GENEPLUS TECH +1
Method and device for route optimization of logistics delivery vehicle
InactiveCN105046365AImprove fitnessShort delivery distanceForecastingLogisticsLogistics managementDistance matrix
The invention discloses a method and a device for route optimization of logistics delivery vehicle, and belongs to the technical field of logistics. The method comprises the following steps of: initializing a congestion matrix alpha and a distance matrix D, generating a delivery route weight matrix omega=alpha D, and initializing a population module N<ZQ>; selecting a population size N<X>, a maximum number of generations N<G>, a crossing-over rate beta, a mutation rate gamma and a number of generations n=0, generating an initial route r1 through a greedy algorithm, and performing mutation operation on the initial route r1 to generate N<ZQ>-1 new routes; calculating fitness A<n> of each route of a first generation population formed by the initial route and the new routes, selecting N<X> routes with the highest fitness from the current population by adopting selection operators, and performing crossover and mutation operations on the N<X> routes to generate a population of next generation; updating n=n+1, when n=N<G>, calculating the fitness A<n> of all the routes in the latest population, and selecting the delivery route with the highest fitness in the current population as the optimal route. According to the invention, when the logistics delivery vehicle delivers goods, the delivery time can be as less as possible, and the delivery route can be as short as possible.
Owner:余意 +3
Automatic calibration parameter optimization method of engine based on genetic algorithm
InactiveCN102337979AStrong automationImprove objectivityElectrical controlMachines/enginesAlgorithmMetapopulation
Owner:ZHEJIANG UNIV
Fluorescent labeling composite amplification kit for 30 STR (short tandem repeat) loci of human Y chromosome and application of kit
InactiveCN106148552AIncrease the number of testsRaise the possibilityMicrobiological testing/measurementFluorescenceY-STR
The invention discloses a fluorescent labeling composite amplification kit for 30 STR (short tandem repeat) loci of human Y chromosome and application of the kit. It is possible to multiple amplify 30 low-mutation-rate STR loci on Y chromosome in single reaction; by designing locus arrangements and specific primer sequences, 30 loci are divided into 5 groups, and six-color fluorescent system marks are used; the Y-STR kit is the domestically first one employing six-color fluorescent detection technology and is an STR kit detecting a highest number of loci, all the loci included are low-mutation-rate Y-STR loci, and the kit is more suitable for examining male families; the composite amplification kit is good in primer specificity, can tolerate a wide range of temperatures and is highly adaptive to subject materials.
Owner:AGCU SCIENTECH
Mutations of the PIK3CA gene in human cancers
ActiveUS8026053B2Inhibit progressReduce the amount requiredMicrobiological testing/measurementAntineoplastic agentsHuman cancerKinase
Phosphatidylinositol 3-kinases (PI3Ks) are known to be important regulators of signaling pathways. To determine whether PI3Ks are genetically altered in cancers, we analyzed the sequences of the P13K gene family and discovered that one family member, PIK3CA, is frequently mutated in cancers of the colon and other organs. The majority of mutations clustered near two positions within the P13K helical or kinase domains. PIK3CA represents one of the most highly mutated oncogenes yet identified in human cancers and is useful as a diagnostic and therapeutic target.
Owner:THE JOHN HOPKINS UNIV SCHOOL OF MEDICINE
Mutation type and mutation frequency of hereditary hearing loss gene SLC26A4 in Chinese crowd and usage of mutation type
InactiveCN101445799AImprove diagnostic efficiencyMicrobiological testing/measurementFermentationMutation frequencyCrowds
The invention relates to 86 types of particular mutation type atlases of SLC26A4 gene related to hearing loss in Chinese crowd, 27 types of relative hot-spot mutation type atlases and frequency information thereof, 13 types of hotspot mutation type atlases and frequency information thereof, and 2 types of hottest-spot mutation type atlases and frequency information thereof. 59 SLC26A4 gene mutation types are newly discovered in the Chinese crowd, wherein, 47 mutation types lead to the change of encoded protein amino acid of the SLC26A4 gene or influence genetic transcription and translation, 6 mutation types lead to base change rather than the change of amino acid, and 6 types are intron mutation types of the SLC26A4 gene. The discovery has a vital practical significance in developing an SLC26A4 gene diagnosing chip and a kit, which conform to hereditary features of the Chinese crowd suffering hearing loss.
Owner:韩东一 +1
Method for generating diversity
InactiveUS20050026246A1Increase probabilityAnimal cellsSugar derivativesGenes mutationAntibody-Producing Cells
The invention relates to a method for preparing an antibody-producing cell line capable of directed constitutive hypermutation of a specific nucleic acid region, comprising the steps of: a) screening a clonal cell population for V gene diversity; b) isolating one or more cells which display V gene diversity and comparing the rate of accumulation of mutations in the V genes and other genes of the selected cells; and c) selecting a cell in which the rate of V gene mutation exceeds that of other gene mutation.
Owner:UK RES & INNOVATION LTD
A molecule adaptor and applications thereof
ActiveCN106906211AAvoid hydrolysisEasy to markMicrobiological testing/measurementLibrary linkersMutation frequencyComputational biology
A molecule adaptor having characteristics of high stability, a high sample DNA connection efficiency and correction functions is designed based on illumina sequencing adaptor optimization. The molecule adaptor can detect mutation sites the mutation frequency of which is as low as 0.05%. The molecule adaptor is used for identifying real mutations in a sample sequencing library constructing process and false-positive mutations introduced by an operating process. In addition, a method of constructing a sequencing library for a sample to be detected is provided.
Owner:SUZHOU PREMED MEDICAL LAB CO LTD +1
Antibodies and methods for generating genetically altered antibodies with high affinity
InactiveUS7235643B2Increase variabilityBetter pharmacokinetic profileAnimal cellsSugar derivativesGenetic diversityMonoclonal antibody
Dominant negative alleles of human mismatch repair genes can be used to generate hypermutable cells and organisms. By introducing these genes into cells and transgenic animals, new cell lines and animal varieties with novel and useful properties can be prepared more efficiently than by relying on the natural rate of mutation. These methods are useful for generating genetic diversity within immunoglobulin genes directed against an antigen of interest to produce altered antibodies with enhanced biochemical activity. Moreover, these methods are useful for generating antibody-producing cells with increased level of antibody production. The invention also provides methods for increasing the affinity of monoclonal antibodies and monoclonal antibodies with increased affinity.
Owner:EISAI INC
Virus identification method and device
ActiveCN106033502AImprove the detection rateImprove accuracyBioreactor/fermenter combinationsBiological substance pretreatmentsAgricultural scienceHomologous sequence
The invention discloses a virus identification method comprising the following steps: obtaining RNA sequencing data of a to-be-tested sample; assembling the first portion of the sequencing data so as to obtain assemble sequences; comparing the first portion of the sequencing data with the assemble sequences so as to obtain a comparison result; determining mutation sites on the assemble sequences according to the comparison result, and determining at least one of the following assemble sequences a-c: a, at least one from the group formed by average entropy and median entropy, and mutation site proportion; b, at least one from the group formed by the average mutation rate and median mutation rate, and the mutation site proportion; c, mutation site proportion; comparing at least one from the assemble sequences a-c with the corresponding boundary, and determining the assemble sequence falling into the boundary to be from virus. The invention also discloses a virus identification device; the virus identification method and / or device can accurately predict whether an unknown sequence is a virus sequence or not without depending on the homologous sequence alignment.
Owner:深圳华大因源医药科技有限公司
Exome potential pathogenic mutation detection method based on family line
InactiveCN105925685ASolve the problem of mining potential pathogenic variantsImprove heterogeneityMicrobiological testing/measurementBiostatisticsFiltrationSingle mutation
The invention provides an exome potential pathogenic mutation detection method based on a family line. The detection method comprises the following steps: 1) reading a result file of an exome sequencing data processing flow, and conducting function filtering; 2) reading the file obtained in the last step, extracting mutations in all samples, calculating a union set, and then combining all samples, so that a matrix is constituted; 3) extracting mutation information in the matrix obtained in the last step, enumerating and assessing pathogenicity of single mutation and pathogenicity of combined dual-site mutation, so that a potential pathogenic mutation list is obtained; and 4) in accordance with the list obtained in the last step, calculating the appearance situations of sites in various samples and target genes. According to the method disclosed by the invention, data integration and basic filtration are completed by taking an output result of the common exome sequencing processing flow as an input condition; by virtue of a special mutation screening algorithm, a candidate set of the potential pathogenic mutations is provided; and the method focuses on solving a problem on potential pathogenic mutation mining of sequencing data with high heterogeneity, high mutation rate and high noise.
Owner:WANKANGYUAN TIANJIN GENE TECH CO LTD
Application of Y-STR (Y chromosome-short tandem repeat)
InactiveCN102618630ATools to Enrich ResearchImprove discriminationMicrobiological testing/measurementImage resolutionTandem repeat
The invention discloses application of Y-STR in individual identification. The Y-STR is selected from any one of or a combination of over any two of DYG1-DYG36. The invention also discloses a detection kit and a detection method used for individual identification. The invention applies 36 new Y-STR loci with high mutation rates effectively in individual identification, and the resolution and accuracy are substantially raised.
Owner:GENESKY BIOTECH INC
Method for generating diversity
InactiveUS7122339B2Increase probabilityAnimal cellsMicrobiological testing/measurementAntibody-Producing CellsAntibody
The invention relates to a method for preparing an antibody-producing cell line capable of directed constitutive hypermutation of a specific nucleic acid region, comprising the steps of: a) screening a clonal cell population for V gene diversity; b) isolating one or more cells which display V gene diversity and comparing the rate of accumulation of mutations in the V genes and other genes of the selected cells; and c) selecting a cell in which the rate of V gene mutation exceeds that of other gene mutation.
Owner:UK RES & INNOVATION LTD
Fluorescence labeling composite amplification kit for human Y chromosome 27 STR gene loci
ActiveCN104164504AImprove featuresHigh sensitivityMicrobiological testing/measurementUnique geneFluorescence
The invention discloses a fluorescence labeling composite amplification kit for human Y chromosome 27 STR gene loci, which can simultaneously amplify 27 Y chromosome STR gene loci (including 24 low-mutation-rate Y chromosome STR gene loci and 3 high-mutation-rate Y chromosome STR gene loci) in a single multiplex reaction. By designing the unique gene locus configuration mode and specific primer sequences, the 27 gene loci are divided into 4 groups which are used for different fluorescence labelings. The composite amplification system has the advantages of wide temperature resistance range, high adaptability of the detection material, and high identification capacity for irrelevant and close relative male individuals.
Owner:GUANGDONG HUAMEI ZHONGYUAN BIOLOGICAL SCI & TECH +1
System and method for monitoring municipal saprobia inhibitive ability in real time based on oxygen uptake rate (OUR)
ActiveCN102520016AGuaranteed to be airtightEliminate distracting factorsMaterial analysis by electric/magnetic meansSiphonProgrammable logic controller
A system for monitoring municipal saprobia inhibitive ability in real time based on oxygen uptake rate (OUR) comprises an aeration siphon device communicated with a sewage supplying system and a sludge supplying system. The aeration siphon device is composed of an aeration groove and a siphon groove in adjoining mode, the wall of the aeration groove is higher than that of the siphon groove, the aeration groove is communicated with the sewage supplying system and the sludge supplying system, and the siphon groove is communicated with an inlet of a closed coiler flow-pushing type bioreactor through a siphon tube. A dissolved oxygen (DO) detection system detects DO values of the inlet and an outlet of the closed coiler flow-pushing type bioreactor, and the signal output end of the DO detection system is connected onto a programmable logic controller (PLC) control system which is connected with an alarm system. The core part of system for monitoring municipal saprobia inhibitive ability in real time adopts the closed coiler flow-pushing type bioreactor so as to monitor the DO value of the inlet and the DO of the outlet in real time, automatically calculate mutation condition of activated sludge OUR, determines OUR mutation rate with different inhibition degree through plenty of experiments, and judge inhibitable degree of the saprobia by comparing automatically calculated OUR mutation rate with a preset value.
Owner:TSINGHUA UNIV +1
Oriented domestication and breeding of methylotrophic bacterium capable of producing pyrroloquinoline quinone at high yield
ActiveCN105624084AIncrease the rate of positive mutations in breedingImprove detection efficiencyBacteriaMicroorganism based processesMicrobiologyPyrroloquinoline quinone
The invention discloses oriented domestication and breeding of methylotrophic bacterium capable of producing pyrroloquinoline quinone at high yield. The invention provides an oriented domestication and breeding method for preparing a methylotrophic bacterium mutant strain capable of producing pyrroloquinoline quinone at high yield. The method comprises the following steps: 1) carrying out repeated induced culture on an initial methylotrophic bacterium used as a treatment object in a methanol-containing liquid culture medium to obtain the domesticated bacterium, wherein all the domesticated bacteria constitute a domesticated strain library; 2) fermenting the domesticated bacteria in the domesticated strain library, and collecting all the domesticated bacterium fermentation liquids; and 3) detecting pyrroloquinoline quinone in all the domesticated bacterium fermentation liquids, and selecting the single strain, the fermentation liquid of which has higher pyrroloquinoline quinone yield than the initial methylotrophic bacterium fermentation liquid, thereby obtaining the methylotrophic bacterium mutant strain capable of producing pyrroloquinoline quinone at high yield. The methanol concentration in the culture medium is gradually enhanced to perform stress domestication on the methylotrophic bacterium, thereby enhancing the breeding direct mutation rate and the pyrroloquinoline quinone yield increase amplitude.
Owner:福建力多利生物科技有限公司
Method for creating new peanut specie
InactiveCN102246692ABeneficial mutation rate increasedLow costPlant tissue cultureHorticulture methodsRootstockGermplasm
The invention relates to a method for creating a new peanut specie, belonging to the technical field of the peanut genetic breeding method. The method adopts radiation mutation to create a new specie. The method comprises the following steps: the embryo of a mature seed is used as the radiation material; after the mutation, the mutated embryo is used to perform tissue culture and obtain a tissue culture regeneration seedling, and the tissue culture regeneration seedling is grafted in a field by using a seedling as rootstock. The beneficial mutation rate of the new specie obtained by the method is up to more than 30%. By using the method, the problem of the traditional method that the beneficial new peanut specie is difficult to create is solved, and the method has remarkable social and economic benefits.
Owner:杨庆利
Genetic algorithm by employing guided local search for multi-objective optimization problem
InactiveCN106611219ADiversity guaranteedAvoid prematureGenetic algorithmsClosest relativesOptimization problem
The invention provides a genetic algorithm by employing guided local search for a multi-objective optimization problem. The algorithm is used for the field of flexible job-shop scheduling. A flexible job-shop scheduling problem belongs to an NP-Hard problem, optimization of multiple objectives often needs to be faced in real production, and the objectives are in interaction and conflict. The genetic algorithm aims at solving the problems of too fast convergence, insufficient population diversity and over-high calculation cost of enumerating all neighborhood solutions caused by continuous cross breeding of close relatives in a genetic operation in the genetic algorithm in the prior art. According to the algorithm, a procedure of calculating a crossover rate and a mutation rate before genetic crossover and mutation and a procedure of searching a movable process and a feasible position by using the guided local search are designed for these problems; and through introduction of the two procedures, the calculation cost is reduced while algorithm premature is avoided. The algorithm is high in practicability and can be well used for actual job-shop scheduling.
Owner:SICHUAN YONGLIAN INFORMATION TECH CO LTD
Chinese population phenylketonuria PAH (phenylalanine hydroxylase) gene screening kit
InactiveCN103436616AFacilitate judgment and identificationLow costMicrobiological testing/measurementCapillary electrophoresisElectrophoresis
The invention discloses a kit for synchronously detecting 12 mutation hotspots of the Chinese population phenylketonuria PAH (phenylalanine hydroxylase) gene. In the kit, 12 loca on the Chinese population PAH gene are used as detection objects; an amplification primer and an extension primer are respectively designed for the mutation type of each locus; each destination section is subjected to PCR amplification and mark extension at the same time; and the gene types of the 12 mutation hotspots are obtained at the same time through capillary electrophoresis analysis. The invention provides a phenylketonuria PAH gene mutation screening kit which is simple, has the advantages of high flux, high performance, low cost and high detection accuracy, is suitable for the Chinese people and suitable for the group screening of the Chinese population phenylketonuria.
Owner:SUZHOU MUNICIPAL HOSPITAL
Who we serve
- R&D Engineer
- R&D Manager
- IP Professional
Why Patsnap Eureka
- Industry Leading Data Capabilities
- Powerful AI technology
- Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.
© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com