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Nucleic acid aptamer of aflatoxin B1 and application thereof

The invention relates to a nucleic acid aptamer which is in high-specificity and high-affinity combination with aflatoxin B1 and application thereof. The nucleic acid aptamer can be used for detection analysis and separating enrichment of aflatoxin B1, and has the advantages of being good in specificity, high in affinity, homogeneous in quality, good in stability, short in development cycle, low in production cost and convenient to use. The invention further relates to derivation sequences of a nucleic acid aptamer sequence and comprises sequences after modification.
Owner:CHINA NAT ACAD NANOTECH & ENG

Nucleic acid aptamer and screening method thereof, and application of nucleic acid aptamer in prostate cancer cell strain detection

The present invention discloses a nucleic acid aptamer, wherein the sequence of the nucleic acid aptamer comprises a DNA segment represented by any one sequence selected from a sequence 1, a sequence 2 and a sequence 3. The nucleic acid aptamer can further be various similar sequences with high homology or a derivative obtained from the sequence of the present invention. The invention further discloses a nucleic acid aptamer screening method, which comprises: synthesizing a random single-stranded DNA library and primers, carrying out SELEX screening, carrying out PCR amplification of library, preparing a DNA single strand library, and finally carrying out repeated screening, negative screening and multi-round screening to obtain the nucleic acid aptamer. The nucleic acid aptamer and the derivative thereof can be used in recognition of the prostate cancer cell strain PC-3 or preparation of kits, molecular probes and targeted mediums for prostate cancer detection, and can further be used in design and preparation of prostate cancer treatment drugs. Compared with the protein antibody, the nucleic acid aptamer of the present invention has advantages of high affinity, high specificity, no immunogenicity, capability of being chemically synthesized, small molecular weight, stability, easy storage, easy labeling and the like.
Owner:GUANGZHOU SHIWEN BIOTECHNOLOGY CO LTD

Nucleic acid aptamer with high specificity and high affinity to human breast carcinoma tissue, preparation method and application thereof

The present invention relates to one kind of nucleic acid gamete with high specificity and affinity to human breast cancer tissue and its preparation process and application. The nucleic acid gamete includes one DNA sequence and one RNA sequence and may be combined to human breast cancer tissue specifically, so that it may be applied for diagnosis and targeting treatment of human breast cancer. The nucleic acid gamete has high specificity and affinity to human breast cancer tissue and no reaction to health mammary gland tissue. The present invention also relates to derived sequences, including modified sequence, of the nucleic acid gamete.
Owner:CHINA NAT ACAD NANOTECH & ENG +1

Silver alloy reflective films for optical information recording media, silver alloy sputtering targets therefor, and optical information recording media

A silver alloy reflective film is used in an optical information recording medium and contains silver as a main component, a total of 1 to 10 atomic percent of at least one rare-earth element, and a total of 1 to 15 atomic percent of at least one selected from In, Sn, Al, and Mg, in which the total content of the at least one rare-earth element and the at least one selected from In, Sn, Al, and Mg is 5 atomic percent or more. The silver alloy reflective film preferably further contains 0.01 to 3 atomic percent of at least one of Bi and Sb. A silver alloy sputtering target has the same composition as the silver alloy reflective film.
Owner:KOBE STEEL LTD

Plant cultivation method

The invention discloses a plant cultivation method which includes the step: cultivating tissue culture seedlings acquired by tissue culture in an energy-saving greenhouse or energy-saving planting greenhouse. A tissue culture process includes the steps: collection of explants; cleaning; disinfection; inoculation of the explants; subculture; rooting culture; transplant. The energy-saving greenhouse or energy-saving planting greenhouse comprises a solar power system, a spray irrigation system and a lighting system. According to the method, horticultural facilities applied in plant cultivation process are uniformly improved and comprise the solar power system, the lighting system, the spray irrigation system, a cleaning and disinfection device, a culture medium filling device and the like, wherein the cleaning and disinfection device and the culture medium filling device are used for the tissue culture process, the whole plant cultivation process is more complete by the aid of a series of improvements and combinations, working efficiency is improved, and each improvement is quite important.
Owner:杭州树联生态工程有限公司
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