161 results about "High homology" patented technology

The present invention relates to an alkaline protease having a prepro sequence, wherein the prepro sequence is a mutant sequence of the amino acid sequence of SEQ ID NO: 1 or an amino acid sequence having 80% or higher homology to the amino acid sequence of SEQ ID NO: 1, in which amino acid residues at (a) position 52, (b) position 75, and (c) position 142 of SEQ ID NO: 1, or amino acid residues at positions corresponding to these positions are substituted by the following amino acid residues:at position (a): aspartic acid or arginine,at position (b): alanine or arginine, andat position (c): lysine;and the alkaline protease, when in a mature form, has the amino acid sequence of SEQ ID NO: 2 or an amino acid sequence having a homology of 80% or higher to this amino acid sequence. The present invention also relates to, for example, a gene encoding the alkaline protease.According to the present invention, an alkaline protease increased in production can be produced. In particular, there can be efficiently produced an alkaline protease having an activity even in the presence of a fatty acid of high concentration and exhibiting excellent detergency against complex soils containing proteins and sebum.

The invention discloses a keratinase mutant with improved thermal stability and a preparation method thereof, and belongs to the field of enzyme engineering. Thermal stability and substrate specificity of the keratinase are improved by interchanging N ends or C ends of two different keratinases with higher homology from the same bacterial strain. The keratinase and the mutant thereof can effectively hydrolyze water-insoluble keratinase substrates such as feathers, wools, and the like, and can be used for leather spinning industry and feed industry.

The invention discloses lncRNA and application thereof as a detection marker or a prognosis recurrence marker for breast cancer combined with lung cancer. The lncRNA is at least one of the following nucleic acid molecules: (1) nucleic acid molecules with cDNA sequences as shown in SEQ ID NO.1, SEQ ID NO.2 or SEQ ID NO.3; and (2) nucleic acid molecules with 90% and higher homology with the nucleic acid molecules with the cDNA sequences as shown in the SEQ ID NO.1, SEQ ID NO.2 or SEQ ID NO.3. According to the lncRNA and application thereof, a group of lncRNAs are screened out as the molecular markers for the breast cancer combined with lung cancer, associated primers are optimized for preparing primer probe chips, and the lncRNA has the advantages of low detection cost, high sensitivity and the like; and the lncRNA and application thereof take the breast cancer combined with lung cancer, such combined cancer, as an example, and take the lead in filling up the blank in research of the combined biological marker lncRNA in China. The lncRNA and application thereof adopt a group of lncRNAs as associated biological markers for combined cancer, and can effectively improve the clinical diagnosis and prognosis evaluation of the combined cancer for instructing clinical treatment.

The invention relates to a diabetes treatment composition based on traditional Chinese medicine nutritional therapy and application thereof, wherein the diabetes treatment composition mainly comprisesvarious natural materials including medicinal and edible materials. The diabetes treatment composition is a low-GI composition, comprising various compound ingredients which may present through carbohydrates, fats and dietary fiber; by usage each day, the dietary fiber in the composition accounts for 10-40 g; the diabetes treatment composition has the total energy of 400-1300 calories, wherein energy provided according to the present quantity of carbohydrates is 15-45% of the total energy of the composition herein; energy provided according to the present quantity of fat is 40-80% of the total energy of the composition, wherein the mass of unsaturated fatty acids is 50-80% of total mass of fat. The composition provided herein lays emphasis on the here-low three-high structure of low GI, low calorie, low available carbohydrates, high unsaturated fatty acids, high dietary fiber and high homology of medicine and food; the intermittent fasting means helps reduce the glucose level of a diabetic patient effectively.

The invention discloses a DNA sequence expressing a truncated treponema pallidum membrane antigen and an amino acid sequence The membrane antigen is removed of the part having high homology with human fibronectin, so as to avoid false positive and improve the specificity of the serological test for diagnosis of treponema pallidum infection. The invention also discloses the application of the membrane antigen in preparing diagnostic reagents for detecting treponema pallidum infection.

The present invention discloses a bridge type fluorescent probe with a bridge type sequence zone doping into mismatched bases and application and a method, the bridge type fluorescent probe 5'-end to 3'-end nucleic acid sequence successively comprises a recognition sequence zone complementary to a nucleic acid sequence of a to-be-tested mutant gene, the bridge type sequence zone, and an anchor sequence zone, wherein the anchor sequence zone is complementary to the outer side sequence zone of 3'-end of the to-be-tested mutant gene, the 3'-end of the anchor sequence is connected with an extension blocking group ; the bridge type sequence zone is a nucleotide derivative sequence containing at least one mismatched bases, an interactiveness fluorescent marking system is marked between the recognition sequence zone and the bridge type sequence zone, the bridge type fluorescent probe is simple in design, can specifically recognizes the gene, is high in sensitivity, and can be used to detect single base mutation and identify highly homologous species and subtypes.

The invention provides a protein-ligand binding site predicting method based on inquiry drive. The method includes the steps of firstly, for an given inquiry input, searching protein sequences with high homology to form a training data set based on inquiry drive; secondly, extracting all the binding residues in the training data set as the positive sample set and extracting all the non-binding residues in the training data set as the negative sample set; thirdly, extracting the feature vector of each sample from evolution information and secondary structure perspective to obtain the feature vector sets of the positive and negative samples; fourthly, using a standard support vector machine algorithm for training to obtain an SVM prediction model based on the inquiry input q; fifthly, for the inquiry input, using the same feature extracting method to extract the feature vector of each residue, inputting the feature vector of each residue into the SVM prediction model, and predicting by using a threshold segmentation method. By the method, prediction precision can be increased, and the possible problems of over-optimization and over-fitting on the fixed training data set can be prevented.

Methods of using polymerase chain reactions to enrich a target sequence in a sample containing reference sequences and target sequences having high homology and amplifiable by the same primer pair are provided herein. In particular the methods provide a robust means to improve the fold enrichment of the target sequence and minimize reaction-to-reaction, well-to-well and run-to-run variations in the enrichment methods.

A homology retrieval can be performed with higher accuracy than conventional technologies when comparing a query sequence with a target sequence, and retrieving a similar location in the target sequence. The sequence information of a query sequence and a genomic-scale target sequence is acquired, the acquired information is compressingly converted into a compressed query sequence and a compressed target sequence in each of which a homopolymer region including two or more consecutive identical bases is replaced with a single base of the bases, the two sequences are compared, and a refining search is performed for a compressed target partial sequence that matches the compressed query sequence in the compressed target sequence. For the refined compressed candidate sequence and the query sequence, based on the information on the number of consecutive identical bases in the each of the sequences before compression, the number of consecutive bases is compared between the two compressed sequences for each corresponding base, and the degree of similarity indicating homology of the candidate sequence with the query sequence is computed from a degree of match or a degree of mismatch in the number of consecutive bases. By ranking and selecting an arbitrary number of candidate sequences having relatively high homology with the query sequence from this degree of similarity, it is possible to avoid the influence of the number of consecutive identical bases in a homopolymer region, thereby performing a homology retrieval accurately.

The invention discloses a novel heat shock protein with high homology to chloroplast elongation factor EF-Tu. Also disclosed is a transgenic method for enhancing tolerance to heat and drought in female reproductive organs. It involves the temporal and spatial expression of novel heat shock EF-Tu in a plant organ or plant tissue. The invention also includes expression constructs, and methods for the production of crop plants with heritable phenotypes which are useful in breeding programs designed to increase heat and drought tolerance.

The invention discloses an EPSP synthase gene derived from ochrobactrum anthropi and application thereof. The EPSP synthase gene contains 1353 basic groups and 451 coded amino acids, the nucleotide sequence of the EPSP synthase gene is as shown in SEQ ID NO1, and the amino acid sequence of the coded proteins is as shown in SEQ ID NO2. The EPSP synthase gene derived from ochrobactrum anthropi disclosed by the invention is synthesized by a manual method, has high homology with the reported EPSP synthase gene derived from agrobacterium tumfaciens, has higher glyphosate tolerance, and can be used for culturing genetically modified crops.

The Magnaporthe grisea MoLON1 gene function and application thereof belong to the field of bioengineering technology, and the invention relates to the function in the pathogenic processes of Magnaporthe grisea MoLON1 gene and purification and purpose of coding proteins thereof. The gene and the ATP-dependent protease Lon1 in yeast, Escherichia coli and other organisms have high homology, and the gene codes 1119 amino acids and contains one intron and two exons. The knockout of MoLON1 gene leads to minimizing of Magnaporthe grisea aerial hyphae quantity, reduction of conidiospore output, basic deprivation of expansion capability of infective hyphae in the paddy rice leaf cells, and improvement of sensitivity to a plurality of adverse conditions; the invention also relates to expression and purification of proteins coded by the MoLON1 gene, and gene can be used as a candidate target for designing and screening anti-Magnaporthe grisea novel medicament.