The invention discloses a quadruple
fluorescence quantitive PCR
typing detection method for common
human papillomavirus infection, which comprises a
fluorescence quantitive PCR technology-based quadruple PCR
reaction system and contains forward and reverse primers of four kinds of
human papillomavirus (HPV) genotypes and AllGlo
fluorescence probes, wherein the capacity of carrying
DNA at most comprising HPV-6,-11, -16 and -18 can be synchronously detected in a
reaction tube under a proper PCR condition. The method comprises the following steps of: designing
specific primers and the fluorescence probes; building a standard molecule; preparing a
standard curve; and detecting a clinical sample. The method can conveniently, quickly and synchronously detect four types of clinically common
HPV infection in the
reaction tube, realizes
typing detection of the four types of HPV by a single-tube PCR at the same time, can quantitatively detect, is simple and quick in operation, high in sensitivity, good in specificity and
repeatability and accurate and reliable in the result, and has very high
clinical value on early preventing and treating woman cervical
carcinogenesis caused by pointed condyloma and the common HPV, blocking infectious source, decreasing
HPV infection and monitoring
clinical treatment effect.