The invention discloses a method for detecting
ureaplasma urealyticum and tiny urealyticum, which belongs to the technical field of detection of microporous plates. The method for detecting
ureaplasma urealyticum and tiny urealyticum has the characteristics that clinical samples processed by
thermal denaturation after PCR (
polymerase chain reaction) amplification are respectively added to
micro holes of
a DNA-BINDING96 hole determination plate equipped coated with Uu and Up specific probes, and
positive control holes are arranged, to which Uu and Up
serotype standard strains processed by
thermal denaturation after PCR amplification are added; after
thermostat hybridization, the plate is cleaned and is patted to be dry;
streptavidin-
horseradish peroxidase conjugates are added to each hole, the plate is cleaned, and the
streptavidin-
horseradish peroxidase conjugates are dried in air; then,
chromogenic substrates A and B are added to each hole, and color development is performed in a shady place after the mixture becomes homogeneous; finally, a stop solution is added, and the result is determined according to color change. The method for detecting
ureaplasma urealyticum and tiny urealyticum not only solves the problem of
false positive result caused by determining a positive result according to color change of a
liquid medium, but also can accurately identify Uu and Up, thereby improving the detection sensitivity. Moreover, high-flux detection is realized, and the method for detecting ureaplasma urealyticum and tiny urealyticum can be widely used for
clinical screening.