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Triple-joint-detection kit for ureaplasma urealyticum, chlamydia trachomatis and neisseria gonorrhoeae

A technology of Chlamydia trachomatis and Ureaplasma urealyticum, applied in biochemical equipment and methods, measurement/testing of microorganisms, microorganisms, etc., can solve problems such as unclear pathogenic mechanism of Uu

Pending Publication Date: 2019-10-25
DAAN GENE CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The pathogenic mechanism of Uu is still unclear

Method used

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  • Triple-joint-detection kit for ureaplasma urealyticum, chlamydia trachomatis and neisseria gonorrhoeae
  • Triple-joint-detection kit for ureaplasma urealyticum, chlamydia trachomatis and neisseria gonorrhoeae
  • Triple-joint-detection kit for ureaplasma urealyticum, chlamydia trachomatis and neisseria gonorrhoeae

Examples

Experimental program
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Effect test

Embodiment 1

[0109] Example 1, detection limit test of Ureaplasma urealyticum, Chlamydia trachomatis and Neisseria gonorrhoeae triple detection kit

[0110] Dilute the inactivated cultures of Ureaplasma urealyticum, Chlamydia trachomatis, and Neisseria gonorrhoeae to a concentration of 10 5 copies / ml, serially diluted to 10 4 、10 3 , 500, 250, 100copies / ml, each concentration sample was added to the final concentration of 10 4 Copies / ml of the plasmid bacteria containing the amplified fragment of the internal standard was used as the sample to be tested, and the sensitivity of the 3-fold detection reagent was tested.

[0111] Test results such as figure 1 , 2 , 3, the results show that the multiple detection system of the present invention can reach 250copies / ml for the detection sensitivity of Ureaplasma urealyticum, the detection sensitivity for Chlamydia trachomatis can reach 250copies / ml, and the detection sensitivity for Neisseria gonorrhoeae can reach 250copies / ml ml.

Embodiment 2

[0112] Example 2, Specificity Test of Triple Detection Kit for Ureaplasma urealyticum, Chlamydia trachomatis and Neisseria gonorrhoeae

[0113] With normal saline, human papillomavirus, herpes simplex virus type Ⅰ, herpes simplex virus type Ⅱ, Neisseria meningitidis, Brevibacterium haemophilus, Escherichia coli, Epstein-Barr virus, and Staphylococcus aureus as specific reference substances, the test solution Specificity of a triple test kit for Ureaplasma ureaplasma, Chlamydia trachomatis and Neisseria gonorrhoeae.

[0114] Test results such as Figure 5 and Figure 6 As shown, the test results show that the multiplex detection system of the present invention has good specificity, and there is no non-specific amplification reaction for non-target pathogens and negative control (normal saline). Test-specific reference products (normal saline, human papillomavirus, herpes simplex virus type Ⅰ, herpes simplex virus type Ⅱ, Neisseria meningitidis, Brevibacterium haemophilus, Esc...

Embodiment 3

[0115] Example 3, precision test of triple detection kit for Ureaplasma urealyticum, Chlamydia trachomatis and Neisseria gonorrhoeae

[0116] Dilute the inactivated cultures of Ureaplasma urealyticum, Chlamydia trachomatis, and Neisseria gonorrhoeae to 10 5 and 10 3 Copies / ml was used as a precision reference product, each repeated 10 times, and the coefficient of variation of each concentration precision reference product was calculated.

[0117] The test results are as Figure 7 , Figure 8 ,and Figure 9 As shown, the test results show that the multiple detection system of the present invention has better precision, and there is no significant difference in different test batches for samples with different concentrations, which can fully meet the requirements of clinical precision. After testing, the coefficients of variation of the high concentration and low concentration precision reference products of Ureaplasma urealyticum were 1.16% and 1.54% respectively; the vari...

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Abstract

The invention provides a triple-joint-detection kit for ureaplasma urealyticum, chlamydia trachomatis and neisseria gonorrhoeae. Specifically, different primers and probes for ureaplasma urealyticum,chlamydia trachomatis and neisseria gonorrhoeae are screened for combined tests, a multiple detection system with excellent specificity and sensitivity for ureaplasma urealyticum, chlamydia trachomatis and neisseria gonorrhoeae is obtained, three kinds of pathogens can be detected by a single tube and tested through a single experiment, and the detection workload is greatly reduced.

Description

technical field [0001] The invention belongs to the field of molecular diagnosis, in particular to a triple detection kit for Ureaplasma urealyticum, Chlamydia trachomatis and Neisseria gonorrhoeae. Background technique [0002] Ureaplasma urealyticum (Uu) is a kind of prokaryotic microorganism without cell wall between bacteria and viruses. At present, there are 14 serotypes known to be related to human diseases, which are divided into two biological groups, that is, U. parvum (UPA) includes serotypes 1, 3, 6, and 14; U. urealyticum ( U.urealyticum, UUR) including the remaining 10 serotypes. Uu widely exists in the genitourinary tract of healthy people, but it is also related to many diseases, such as non-gonococcal urethritis, chorioamnionitis, etc., and can cause urinary system infection and infertility under certain conditions. Ureaplasma urealyticum is mostly parasitic in the male urethra, penis foreskin and female vagina. It can cause prostatitis or epididymitis in ...

Claims

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Application Information

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IPC IPC(8): C12Q1/689C12Q1/04C12N15/11C12R1/36
CPCC12Q1/689C12Q2600/16
Inventor 蒋析文范健刘悦彭海龙廖芷卉
Owner DAAN GENE CO LTD
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