Neisseria gonorrhoeae, Chlamydia trachomatis and Ureaplasma urealyticum detection kits

Abstract

The invention discloses a gonococcus, chlamydia trachomatis and ureaplasma urealyticum detection kit. The detection kit comprises a gene chip (1) and various primers (2), wherein the gene chip carries (i) nucleotide probes of different pathogens, (ii) a biotin point-labeled DNA (deoxyribonucleic acid) sequence and (iii) a DNA sequence carrying beta-globulin encoding part; the nucleotide probes are a sequence of SEQ ID Nos:1-3 or a sequence complementary to SEQ ID Nos:1-3; the biotin point-labeled DNA sequence is SEQ ID No.4; the DNA sequence carrying beta-globulin encoding part serving as an internal control point is SEQ ID No.5; and various primers are used for amplifying the DNA sequences in a clinical sample, and have DNA sequences of SEQ ID Nos.6-11. The kit can quickly and accuratelydetect infections of gonococcus, chlamydia trachomatis and ureaplasma urealyticum, and has magnificent meaning to pathogen detection.

Description

technical field [0001] The invention relates to the field of genes, in particular to a detection kit for Neisseria gonorrhoeae, Chlamydia trachomatis and Ureaplasma urealyticum. Background technique [0002] Neisseria gonorrhoeae, Chlamydia trachomatis and Ureaplasma urealyticum are a group of pathogens that are mainly transmitted by sexual contact, and are also the main pathogens that cause various sexually transmitted diseases clinically except treponema pallidum. They can cause gonorrhea and non-gonococcus respectively Sexual urethritis (cervicitis), these pathogens can also cause concurrent infections when infecting patients, such as HIV infection. [0003] Gonorrhea is one of the most common sexually transmitted diseases in the world, with an increase rate of 20% to 30% every year. Due to the abuse of antibiotics and repeated infections, the clinical manifestations of gonorrhea lack specificity, especially for women, 60% to 70% of the initial infection It presents an a...

AI Technical Summary

Problems solved by technology

[0009] Hybridization methods can improve the specificity of DNA analysis, but most hybridization methods are time-consuming and labor-intensive, and cannot meet the requirements of clinical rapid detection

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