434 results about "Protein chip" patented technology

This invention provides methods of retentate chromatography for resolving analytes in a sample. The methods involve adsorbing the analytes to a substrate under a plurality of different selectivity conditions, and detecting the analytes retained on the substrate by desorption spectrometry. The methods are useful in biology and medicine, including clinical diagnostics and drug discovery.

The present invention relates to methods of conducting assays for enzymatic activity on microarrays useful for the large-scale study of protein function, screening assays, and high-throughput analysis of enzymatic reactions. The invention relates to methods of using protein chips to assay the presence, amount, activity and / or function of enzymes present in a protein sample on a protein chip. In particular, the methods of the invention relate to conducting enzymatic assays using a microarray wherein a protein and a substance are immobilized on the surface of a solid support and wherein the protein and the substance are in proximity to each other sufficient for the occurrence of an enzymatic reaction between the substance and the protein. The invention also relates to microarrays that have an enzyme and a substrate immobilized on their surface wherein the enzyme and the substrate are in proximity to each other sufficient for the occurrence of an enzymatic reaction between the enzyme and the substrate.

The present invention relates to protein chips useful for the large-scale study of protein function where the chip contains densely packed reaction wells. The invention also relates to methods of using protein chips to assay simultaneously the presence, amount, and / or function of proteins present in a protein sample or on one protein chip, or to assay the presence, relative specificity, and binding affinity of each probe in a mixture of probes for each of the proteins on the chip. The invention also relates to methods of using the protein chips for high density and small volume chemical reactions. Also, the invention relates to polymers useful as protein chip substrates and methods of making protein chips. The invention further relates to compounds useful for the derivatization of protein chip substrates.

A substrate comprises a surface, and a plurality of moieties, on at least a portion of the surface. The moieties are moieties of formula:Surf-L-Q-T,where -T comprises a reactant ligand, and Surf- designates where the moiety attaches to the surface. The substrate can be made into a protein chip by the reaction of a reactant ligand and a fusion polypeptide, where the fusion polypeptide includes a capture polypeptide moiety which corresponds to the reactant ligand.

A defect detecting apparatus captures images of a protein chip formed on each die of a wafer at a plurality of different focal positions, with respect to every division region obtained by dividing each die in plurality; stores inspection target images for every division region at every focal position together with an ID for identifying each division region; creates a model image for every division region at every focal position by calculating an average luminance value of pixels of each inspection target image having the corresponding ID; extracts a difference between the model image and each inspection target image as a difference image; extracts a Blob having an area larger than a preset value from each difference image as a defect; and classifies the kind of the defect based on a feature point of the extracted Blob.

This invention relates to preparation and detect method of detection protein chip of diabetes autoimmune antibody. The scheme is as follows: a protein chip is a thin glass chip set with multiple reaction tanks on top surface of the said shell and a detection microarray in each of the reaction tank, every micro-array is composed of three different kinds, same size protein micro-solid phase detection chips of GAP, PTP and Insulin. The preparation method is to prepare protein micro-solid phase detection chips to be assembled and the detecting method is to apply indirect fluorescent method for detection.

The invention discloses a high-throughput multi-index protein chip for screening esophageal squamous carcinoma specific protein markers, and discloses a kit supporting the use. 55 types of esophageal squamous carcinoma differential proteins in the serum or blood plasma are selected and used as the esophageal squamous carcinoma protein markers and protein contrast prepare the protein chip; and the protein chip comprises a substrate, protein detection indicators and a contrast detection indicator coating, wherein the protein detection indicators are distributed as arrays on the substrate. Detection liquid of a supplementary experiment reagent is filled in the kit. By adopting the protein chip, the protein profiles of three types of people, namely, normal people, people subjected to esophageal squamous cell carcinoma precancerous lesions, and people subjected to the esophageal squamous carcinoma, can be determined; and means are provided for screening the esophageal squamous carcinoma in the early stage, diagnosing in mid stage and late stage, as well as monitoring the state of illness.

The present invention discloses a protein chip fully-automated high-throughput analysis apparatus, which comprises a control host machine, a display electrically connected to the control host machine, and a fully-automatic protein chip workstation, wherein the table surface of the fully-automatic protein chip workstation is provided with a sample treatment, a reagent treatment system, a plate washing system and a signal acquisition system, a free mechanical arm and a liquid adding system are added above the table surface of the work station, and the sample treatment system comprises a suction head support frame, a sample support frame, a reagent support frame and a barcode scanner. With the protein chip analysis apparatus of the present invention, the simultaneous detection on the multiple samples, the standard substance and the quality control substance can be achieved, the calculation on the concentration of the current batch of the samples through the current batch of the standard substance and the quality control substance is achieved, and the detection result is accurate and stable; and the multiple samples share the one standard substance or quality control substance so as to achieve the agent saving effect.

A simple and quick protocol for chemical treatment, enzymatic or chemical digestion, and subsequent identification of proteins on protein chip arrays is disclosed, together with kits therefor. The chemical treatment comprises denaturation, reduction and alkylation while enzymatic digestion encompasses deglycosylation, dephosphorylation, and digestion by various proteases. Digestion is also accomplished by various chemicals that are known to induce proteolysis. All reactions are carried out sequentially on chip. Subsequent peptide mass fingerprinting or product ion searches allow the identification of specific peptides which can be correlated to proteins. The method of the present invention can be applied to the analysis of biological samples such as urine and plasma to identify biomarkers in diseased states. The methods of the present invention allow complete on chip treatment, which can be used for rapid protein identification and structural characterization of heavily posttranslationally modified proteins.

The invention discloses a chip preparation method, a DNA or protein immobilization method, and a chip. The chip preparation method of the invention comprises performing weak passivation treatment after immobilization treatment, wherein the weak passivation treatment comprises contacting a weak passivation reaction solution containing a catalyst with a chip subjected to immobilization treatment, soas to promote binding of DNA or protein to the surface of a substrate, and to enable the DNA or protein to be sufficiently immobilized on the surface of the substrate. The preparation method of the invention increases the weak passivation treatment step after the immobilization treatment, so that the DNA or protein can be more fully immobilized on the surface of the substrate, the quality and efficiency of DNA or protein immobilization are improved, also DNA or protein is more fully immobilized, the relationship between the amount of the DNA or protein immobilized on the chip and the concentration of the DNA or protein initially added is more closely, and thus the immobilization amount of the DNA or protein is highly controllable, the repeatability is good, and a foundation is laid for preparing high-quality DNA or protein chips.