The invention belongs to the technical field of biomedicine automation instruments, and particularly relates to a bacterial colony picking instrument. The bacterial colony picking instrument comprisesa base, a rack of a top plate, a petri dish plane mobile table, a photographic device, an inoculation pore plate plane mobile table, a rotating device, a cleaning and disinfection device, a pluralityof picking devices and a plurality of driving devices, wherein the petri dish plane mobile table is arranged on the base and used for driving a petri dish to move in a plane, the photographic deviceis arranged on the base and used for collecting an image of a bacterial colony in the petri dish, the inoculation pore plate plane mobile table is arranged on the base and used for driving an inoculation pore plate to move in a plane, the rotating device is arranged on the base and can rotate circumferentially, the cleaning and disinfection device is arranged on the base and located below the periphery of a disc, the picking devices are all arranged on the disc and arranged at intervals along the periphery of the disc, and the driving devices drive the picking devices to perform picking action. The bacterial colony picking instrument has the advantages that during the achieving work, the picking efficiency is high, under the cooperation of the photographic device, the identification precision of the bacterial colony is also greatly improved; the whole structure of the instrument is not complicated, and the operation is convenient.