221results about How to "Easy to inoculate" patented technology

The invention discloses a method for using lily to prepare parent group to cultivate grouped and cross, belonging to plant group cultivate and cross technique. The inventive method comprises that preparing cultivate medium, using non-matured blank of lily as explant to be induced, increased, expanded, cultivated, refrigerated, transplanted, and virus detected, and using harmless tube bulb seed to plant in field, flower, and cross, to obtain lots of lily harmless seeds. The invention has the advantages in simple grafting operation, low pollution, high survival rate, low variant rate, high cross purity, high seed quality, shortened induce cultivate period, reduce cost and high practicality.

The invention provides an industrialized cultivation method of Morchella importuna. The method is characterized by comprising steps as follows: (1) preparation of a culture substrate; (2) preparation of a liquid strain; (3) mycelium culture; (4) fruiting management: mycelia in the step (3) are transferred to a fruiting room for fruiting management, and the fruiting management comprises management in a primordium formation promoting period, management in a mushroom bud formation period, management in a growth period and management in the manure period. According to the method, crop waste is taken as a raw material for preparation of a culture medium, liquid strains are used for sowing, industrialized cultivation of Morchella is completely realized indoor, the method is simple to operate, low in cost, short in cycle, controllable in quality and capable of realizing industrialized production all year round, and one pollution-free, pleasantly scented and delicious green product is obtained.

The invention discloses a method for preparing edible mushroom liquefied strains. The method comprises three steps, i.e. the purification and culture of primary strains, the preparation of sterilized water and the preparation and liquefaction of secondary strains. According to the method, the primary strains are cultured in a PDA (Potato Dextrose Agar)-enriched culture medium so as to obtain purified primary strains; the purified primary strains are segmented and are then inoculated into a water-soluble culture medium so as to obtain the secondary strains; and hyphae of the secondary strains are broken into pieces by using a high-speed strain crusher, and then, the secondary strains are added to the sterilized water and diluted, thereby obtaining the liquefied strains. The method has the advantages of simple process, simplicity and convenience in inoculation, quickness in strain growth, earliness in mushroom growing, good quality, high yield, high efficiency, low pollution risk, low investment and operating cost and good economic benefit. After the edible mushroom liquefied strains prepared by using the method are inoculated, the permeation of the strains is strong, and the strain growth is quick. Furthermore, the culturing and mushroom growing time of the edible mushroom liquefied strains is greatly shortened, the mushroom growing is orderly, the growing speed is high, and the yield is obviously increased.

The present invention relates to a method for virus-free tissue culture and rapid propagation of sugarcane belonging to the technical field of plant propagation. The method comprises the following steps that the terminal buds or auxiliary buds of the sugar cane is taken as the explants and the virus-free tissue culture seedlings are propagated in mass after the callus tissue inducement and culture, plant differentiation culture, proliferation culture, radication culture and virus test. The present invention is characterized in that the explants adopted are large and the operation is easy; the inoculation survival rate is high; the detoxification is simple, convenient, fast and thorough; the propagation coefficient per month is 5 to 10 times and the speed is fast which is fit for breeding in factory and commercial production.

The invention discloses an Agaricus Bisporus liquid spawn and a preparation method thereof. The Agaricus Bisporus liquid spawn is produced by means of three-level propagation through slant and shake-flask media and a fermenter medium. The slant and shake-flask media comprise components including 100-200g of potato, 50-150g of cow dung, 10-30g of glucose, 1-2.5g of mono potassium phosphate, 0.5-2g of magnesium sulfate and 1-4g of peptone; the fermenter medium of every 50 liters of water is composed of 2-4kg of potato, 1-3kg of cow dung, 0.5-1kg of bran, 0.2-0.4 kg of corn powder, 0.5-1kg of sucrose, 30-60g of mono potassium phosphate, 20-30g of magnesium sulfate, 120-150 mg of vitamin B and 40-50g of defoamer. By application of the Agaricus Bisporus liquid spawn and the preparation method thereof, the problem that mycelia are prone to aging and degradation during growth is solved; the liquid spawn is of globular mycelia with uniform age, high activity and fast running; a high-running spawn center is formed after the liquid spawn is inoculated to solid media, with uniform fruiting achieved; the preparation cost is only one-tenth of that of solid spawn; the growth cycle of fermenter spawn is 7-9 days, and thus spawns on a large scale can be cultured within a short time.

The invention discloses a vaccination information management system based on an internet cloud technology. The vaccination information management system comprises a data acquisition module, an APP client-side, a server, a health consultation recommendation module and a high-quality commodity free recommendation module, the data acquisition module, the APP client-side and the server are connected through the internet, and the server is further connected with the health consultation recommendation module and the high-quality commodity free recommendation module through the internet, wherein a special team is in charge of both the health consultation recommendation module and the high-quality commodity free recommendation module. The vaccination information management system based on the internet cloud technology achieves informatization, intellectualization, facilitation and precision of child vaccination work, achieves the situation that parents give inoculation to a baby accurately, timely, conveniently and satisfactorily and achieves accurate statistics of population data of different age groups in a national big data era.

The invention relates to a method for breeding Pseudostellaria heterophylla by virus-free tissue culture. The method comprises the following steps of: selecting explants, performing induction culture, detecting viruses, performing enrichment culture, performing rooting culture, propagating virus-free tissue-culture seedlings, acclimating, transplanting, and producing virus-free Pseudostellaria heterophylla stock seeds, first generation seeds and second-generation propagation seeds (production seeds). The method is easy to operate and has a low inoculation contamination rate and a high survival rate. The virus elimination operation is simple, rapid and complete, the propagation coefficient is increased by 10-15 times, and the propagation speed is high. The virus-free tissue-culture seedlings are suitable for industrialized production. The root tubers produced from the virus-free tissue-culture seedlings have resistance, are better in yield and quality than virus-infected seedlings, and can be produced commercially.

The invention discloses a bagged liquid strain inoculation machine, which is characterized by comprising a stand, wherein the stand is provided with a conveying roller and a conveyor belt arranged on the conveying roller; a drilling mechanism is arranged on the stand above the conveyor belt and comprises a bracket and a lifting device; the lifting device is connected at the upper part of the bracket; a plurality of drilling rods are connected and arranged at the lower part of the bracket; an inoculation mechanism is arranged on the stand which is positioned above the conveyor belt and at the right side of the drilling mechanism and comprises a bracket body and a lifting device which is connected and arranged at the upper part of the bracket body; and a plurality of inoculation needle heads are connected and arranged at the lower part of the bracket body and are connected with an inoculation pipeline. By adopting a setting mode of separating the drilling mechanism from the inoculation mechanism, the invention realizes the drilling and then the inoculation; and an inoculation bacterial bin is effectively formed on a bacterial bag and is convenient to carry out the inoculation, so that the inoculation quantity is easy to control, the phenomenon of blocking a needle hole cannot generate and the inoculation efficiency is high.

The invention relates to a special matrix formula suitable for culturing mycorrhizal tobacco seedling in the condition of flue-cured tobacco seedling by floating system; all the constituents and the volume thereof are as follows: 60 percent of peat, 20 percent of perlite and 20 percent of vermiculite. The formula can improve the comprehensive quality of the tobacco seedling, cultures sound seedlings and has the advantages of wide material sources, easy acquisition, easy matrix preparation, simple production, simple inoculating operation, little fungicide dosage and easy promotion, etc. The culture medium, the inoculating technique and the corresponding Glomus intraradices Smith and Schenck are combined together, infection rate of mycorrhiza is up to over 40 percent and the effect of the mycorrhiza is good.

The invention provides a method for preventing and treating plant soil-borne diseases and cultivating anti-bacteria cultivation soil. The method comprises the steps that firstly, during the stubble-free period in spring and summer, soil in a greenhouse or a field is deep tilled and is smashed, haulm or other straw and quick lime are evenly scattered to the soil, the soil is tilled again and watered, the soil is pressed tightly after being covered with a plastic film, and then a shed is covered tightly or sunshine soil drying is carried out; secondly, microbial manure or organic compost containing beneficial bacteria is evenly applied to the soil, ditching and ridging are carried out, and plants are prepared to be planted. The method solves the problems that the soil is easily infected by the disease bacteria again after high-temperature shed covering or sunshine soil drying is carried out, and when the microbial manure or the organic compost containing the beneficial bacteria is applied, due to the fact that the number of indigenous microorganisms in the soil is large, the beneficial microorganisms guided by applying the bacterial manure or the organic compost containing the beneficial bacteria are generally not prone to colonization and cannot play a role.