59results about How to "Improve vaccination success rate" patented technology

The invention discloses a method for using lily to prepare parent group to cultivate grouped and cross, belonging to plant group cultivate and cross technique. The inventive method comprises that preparing cultivate medium, using non-matured blank of lily as explant to be induced, increased, expanded, cultivated, refrigerated, transplanted, and virus detected, and using harmless tube bulb seed to plant in field, flower, and cross, to obtain lots of lily harmless seeds. The invention has the advantages in simple grafting operation, low pollution, high survival rate, low variant rate, high cross purity, high seed quality, shortened induce cultivate period, reduce cost and high practicality.

The invention relates to an ornamental ganoderma lucidum cultivation method. The ornamental ganoderma lucidum cultivation method is characterized by including the technological processes of tree selection, tree felling, segment cutting, bag making, sterilization, inoculation, spawn running, primordium inducement, ganoderma lucidum cultivation and modeling, pure broad-leaved tree basswood serves as the material, an ornamental ganoderma lucidum variety is selected, ganoderma lucidum liquid spawns are made, and inoculation is performed with a liquid spawn inoculation method. At the ganoderma lucidum spawn running phase and the ganoderma lucidum sporocarp growing and developing phase, the temperature, the humidity, the illumination and the concentration of carbon dioxide of the environment are comprehensively regulated; at the ganoderma lucidum primordium differentiation phase, the ganoderma lucidum stem elongation phase and the ganoderma lucidum pileus differentiation phase, according to the biological characteristics, including phototaxis, regeneration and sensitivity to carbon dioxide, of ganoderma lucidum, the living ganoderma lucidum is artistically modeled respectively through the key technologies of ganoderma lucidum living body grafting, light source induction and ganoderma lucidum pileus regeneration, and then living ganoderma lucidum representing auspicious omen and having high ornamental value is cultivated. The cultivated ganoderma lucidum is natural and ecological, shows the wonderful workmanship excelling nature and manifests unique art forms and cultural connotation of the ganoderma lucidum, the cultivated ganoderma lucidum not only can serve as an upmarket present for relatives and friends but also can be added to a household art collection, the cultivated ganoderma lucidum has wide application prospects and will create good economic benefits and social benefits.

The present invention relates to a method for virus-free tissue culture and rapid propagation of sugarcane belonging to the technical field of plant propagation. The method comprises the following steps that the terminal buds or auxiliary buds of the sugar cane is taken as the explants and the virus-free tissue culture seedlings are propagated in mass after the callus tissue inducement and culture, plant differentiation culture, proliferation culture, radication culture and virus test. The present invention is characterized in that the explants adopted are large and the operation is easy; the inoculation survival rate is high; the detoxification is simple, convenient, fast and thorough; the propagation coefficient per month is 5 to 10 times and the speed is fast which is fit for breeding in factory and commercial production.

The invention relates to a method of tissue culturing and fast breeding pinellia tuber of toxicity-removing, comprising the following steps: employing the immature seed and / or mature seed of pinellia tuber as explant, breeding large quantity of tissue culturing sprout of toxicity-removing, bulbil of toxicity-removing and small root tuber of toxicity-removing after evoked culturing, enrichment culturing, root growing culturing, replanting and virus detection. The invention is characterized by the simple inoculation, low inoculation pollution rate, high survival rate; simple, fast and through toxicity removing; 5-10 times breeding coefficient, fast breeding, suitable for factory breeding and commercial production.

The invention relates to an inoculator of edible fungus, comprising a steam pressure tank, a gas compressor, two gas filters in series, a gas distributor and a fungus seed storage tank; the inoculator adopts steam generated by the steam pressure tank to carry out sterilization to a pipeline system, and utilizes the gas compressor, the gas filters and the gas distributors to form sterile operating environment so as to implant fungus packs in liquid spawn or colloid spawn in the fungus seed storage tank of the confined steam pressure tank to realize the quantitative inoculation. The invention has the advantages of convenient operation, fast speed, consistent inoculation, no pollution, high efficiency with greatly reducing labor intensity, etc., which is applicable to industrial production and countries of mountainous areas.

The invention discloses edible fungi sterilization and inoculation integrated equipment, comprising a liquid strain fermentation tank and a sealed sterilization warehouse. The external part of the sterilization warehouse is provided with a steam intake pipe, a steam discharge pipe, a water intake pipe, a water discharge pipe, a strain pipe, an air intake pipe, an air discharge pipe, an air filterand a fan; the internal part of the sterilization warehouse is provided with a plurality of layers of supporting frames; water circulating pipes which are arranged in a bending way are arranged on each layer of supporting frames; two ends of the water circulating pipes are respectively connected with the water intake pipe and the water discharge pipe; a plurality of auxiliary inoculation pipes arearranged between adjacent water circulating pipes; the end parts of the auxiliary inoculation pipes are provided with primary inoculation pipes; and the strain pipe is communicated with the liquid strain fermentation tank. The edible fungi sterilization and inoculation integrated equipment has simple structure, reduces the output links, is cooled without the requirement of going out of a pan, hasautomatic inoculation and high inoculation success rate, avoids the pollution brought by the interference of artificial factors, and essentially realizes automation, stabilization and standardization, thereby achieving the purposes of labor and time conservation as well as low pollution rate, and being the equipment with very high degree of automation.

The invention discloses a classical swine fever virus auxiliary fluid for inoculating a rabbit and a preparation method thereof, and belongs to the technical field of biological products for livestock. Every 1000ml of phosphate buffer solutions in the auxiliary fluid comprises the following components in proportions by weight: 6g to 10g of vitamin compositions, 3g to 5g of amino acid compositions, 10g to 30g of compound Chinese medicinal herbal polysaccharides, and 0 to 10mg of sodium selenite. The classical swine fever virus auxiliary fluid for inoculating the rabbit has the positive and beneficial effects that a virus can be delivered to a susceptible organ when the rabbit is inoculated; the inoculation loss is reduced; the inoculation success rate is improved; the infection stability of the rabbit can be improved; batch-to-batch differences are reduced; the batch-to-batch stability of finished-product vaccines is ensured; the quality of the vaccines is improved; the multiplication density of a classical swine fever virus in the body of the rabbit can be specifically improved; in comparison with the prior art, the titer of the virus is increased by 5 to 10 times; the weight of a gained splenic organ is discovered to be slightly increased after the rabbit is inoculated; in comparison with the prior art, the weight of the gained splenic organ is increased by 15 percent to 40 percent.

The invention discloses a virus-free tissue culture and rapid propagation method of castor oil plants. The method comprises the following steps: adopting current year's ripe seeds, mixing castor oil plant seeds with coarse sands according to a ratio of 1:5-8, carrying out medium speed grinding and shelling, screening to remove the coarse sands, cleaning the obtained seeds with warm water 2-3 times, immersing the washed seeds in 75% alcohol for 0.5-1.0min, disinfecting the immersed seeds with an aqueous solution of 2% sodium hypochlorite for 20-30min, and flushing the disinfected seeds with sterile water 3-5 times to obtain the flushed seeds used as explants; and carrying out induction culture, propagation culture, rooting culture, transplanting and virus detection to obtain mass propagated virus-free tissue culture seedlings, virus-free plant buds and virus-free small root tubers. Compared with present castor oil plant seedling growing methods, the method disclosed in the invention has the advantages of low inoculation and pollution rate, high transplanting survival rate and high quality.

The invention provides a fermenting agent suitable for edible fungi and a method for fermenting the edible fungi by using the fermenting agent, and relates to the technical field of edible fungus planting. The fermenting agent suitable for the edible fungi is prepared from 97-99wt% of main materials and 1-3wt% of auxiliary materials; the main materials comprise cottonseed hulls, roxburgh rose residues, honeysuckle straws and crop straws, and the weight ratio of the cottonseed hulls to the roxburgh rose residues to the honeysuckle straws to the crop straws is 0.8: 0.6: 0.3: 0.3; and the auxiliary materials comprise lime, gypsum and a fermentation auxiliary agent, and the fermentation auxiliary agent is an 1-3 wt% EM aqueous solution. The invention further provides a method for fermenting the edible fungi by using the fermenting agent. According to the method, exogenous heat is introduced through a vent pipe and cooperates with the fermentation materials for rapid fermentation, so that the fermentation time is greatly shortened, the field is saved, labor is saved, energy consumption is reduced, and the economic efficiency is improved.

The invention belongs to the technical field of microbial electrochemistry and discloses a construction method of a microbial electrochemical system for hydrogen phosphide generation reinforcement. The construction method comprises mixing animal wastes and anaerobic activated sludge, adding the mixture into an anaerobic reactor, carrying out culture through a medium to obtain a phosphine-producing microorganism-rich domesticated activated sludge mixed solution, taking the domesticated activated sludge mixed solution, carrying out centrifugation separation, carrying out washing through a phosphate buffer solution, adding inoculation sludge into a microbial electrolysis cell, carrying out biofilm culturing, applying power for the microbial electrolysis cell, adding the electrolysis product into a medium and carrying out anaerobic cultivation for hydrogen phosphide generation reinforcement. Through construction of the microbial electrolysis cell and domestication of microbes, a hydrogen phosphide yield of phosphate reduction and a hydrogen phosphide production rate are improved. The construction method has a good application prospect.

The invention relates to an expanding propagation method of phytopathogens, in particular relates to an inoculation expanding propagation method of corn puccinia polysora underw. The inoculation expanding propagation method of the corn puccinia polysora underw comprises the following steps of spraying Tween-20 solution to diseased leaves; eluting spores on the diseased leaves in the Tween-20 solution to prepare a spore suspension solution; inoculating the spore suspension solution to healthy corn seedlings; after culture, harvesting spores obtained by expanding propagation; and performing repeated transfer by using the healthy corn seedlings. The method has the advantages that the expanding propagation speed of the puccinia polysora underw is high, the efficiency is high, a large amount ofhigh-activity puccinia polysora underw spores can be rapidly obtained, the expanding propagation period of the puccinia polysora underw is greatly shortened, and an efficient strain expanding propagation method and technical support are provided for researching southern corn rust.

The invention belongs to the technical field of fungal fertilizer and particularly relates to a mycorrhizal fungi microcapsule seed coating agent capable of improving a saline-alkali soil microbial flora structure as well as a preparation method of the mycorrhizal fungi microcapsule seed coating agent. The seed coating agent particularly comprises the following components: 1 to 1.5 parts of arbuscular mycorrhizal fungi preparation, 0.1 to 0.2 part of trichoderma harzianum preparation, 3 to 5 g of xanthan gum, 1 to 1.2 g of calcium carbonate, 0.1 to 0.2 g of citric acid aqueous solution, 0.2 to0.3 g of ethyl lactate and 80 to 100 mg of polylactic acid. The microcapsule is prepared by a mode of forming compound emulsion by the polylactic acid and the xanthan gum; the prepared seed coating agent has high film-forming property and high embedding rate; the formed capsule wall has high stability, can effectively protect the activity of the fungi, can directly coat the seed and has good slow-release effect; through mutual synergistic effect of the arbuscular mycorrhizal fungi and the trichoderma harzianum, the inoculation success rate is high; and after inoculation, the yield of crops isobviously increased and the morbidity probability of the crops is reduced.

The invention discloses a liquid inoculation method for artificially planting cistanche. The liquid inoculation method includes the following steps of cistanche seed grading, screening and germinating treatment, modifying of an inoculating device, inoculating liquid preparing, inoculating ditch excavating, farmyard manure fertilizing, liquid inoculating, inoculating ditch backfilling and conventional irrigation management after watering for the first time and soil moisture filling. The liquid inoculation method has the advantages that by the liquid inoculation method for cistanche seeds, germination, growing and restoring of host root systems can be promoted, distance between the seeds and the host root systems can be shortened, inoculating section can be expanded to increase contact probability of the seeds and host roots, and inoculating success rate and yield are increased while seed consumption is reduced. Experiments show that compared with methods of direct broadcast sowing and seed paper inoculating, the liquid inoculation method is seed saving, convenient for operation, short in inoculation period and highest in inoculation rate, spray gun equipment with diameter of 2.0mm and used in the method is medium in seed spraying quantity and good in spraying effect, seed consumption is reduced due to spraying, and the liquid inoculation method is high in inoculation rate, convenient for operation during production and easy for popularization.

The invention discloses a method for identifying wheat scab extension resistance through wheat ear top double-flower inoculation. A prepared gibberella spore liquid is inoculated into bilateral florets of a sixth spikelet at the top of a wheat ear by utilizing a double-flower instilling method in a wheat flowering stage, a browning condition of inoculated spikelet is recorded, a diseased spikeletrate of infected spikelet at the lower part of the inoculated spikelet after inoculation is counted, and wheat scab extension resistance is dynamically evaluated. According to the inoculation method,a diseased part mainly expands downwards from the sixth spikelet on the top, the diseased spikelet rate at the lower part of the sixth spikelet is counted, diseased parts are consistent, the problem that identification reliability is affected due to the fact that white spikes are prone to being withered through a traditional single-flower instillation method is solved, and the extension resistanceof varieties can be reflected more accurately.

The invention belongs to the technical field of plant root-knot nematode inoculation methods, and particularly provides a plant root-knot nematode potting three-layer sick soil inoculation method. Themethod comprises the steps of 1, disease-free soil collection and treatment; 2, sick soil collection and treatment; 3, sick soil expanding propagation cultivation; 4, expanded propagation sick soil pretreatment; and 5, three-layer sick soil inoculation. The plant root-knot nematode potting three-layer sick soil inoculation method adopts a three-layer sick soil inoculation technology that disease-free soil is inoculated on upper and lower layers, and nematode sick soil is inoculated on a middle layer. Compared with an existing root-knot nematode egg and larvae inoculation technology, the technology approaches natural inoculation, the inoculation survival rate is high, a nematode selecting link is omitted, and the workload is greatly reduced. Compared with an existing root-knot nematode single sick soil inoculation technology, the technology has the advantages that the usage amount of the sick soil is reduced by 1 / 2-2 / 3, and the workload and cost of the collection, transportation, expanding propagation cultivation and the like of the sick soil are greatly reduced; and newly transplanted seedlings can be protected from being infected by nematodes, not only can the success of the inoculation be ensured, but also too early disease death of the seedlings can be avoided.

The invention discloses a method for measuring pathogenicity of pathogen wood-rotting fungi in basidiomycetes by standing timber stems. The method includes the steps: (1) manufacturing a wood dust culture medium containing 82% of wood dust, 15% of wheat bran, 2% of glucose and 1% of gypsum; (2) introducing bacterial strains into the wood dust culture medium and manufacturing an inoculant stick; (3) selecting a young tree, disinfecting the young tree, cutting a stem 10-30cm away from the ground to form a 1-5cm long and 1-5mm deep notch serving as an inoculation position matched with a shear mouth on one side of the inoculant stick, attaching wet cotton to the lower end of the inoculation position to preserve moisture, fixedly wrapping the inoculation position with a plastic strip, and wrapping the stem about 5-10cm away from the upper portion of the inoculation position with thick paper to form an umbrella to cover the inoculant stick; (4) performing inoculation and infection for 1-2 months, unfastening the thick paper and the plastic strip wrapping an inoculant and verifying the pathogenicity. By the method, the young standing timber stems are inoculated for the first time, the pathogenicity of the pathogen wood-rotting fungi causing tree stem rot and root rot is measured, success rate is high, and operation is simple.

The invention provides a separation and purification method of eimeria tenella sporozoite and an inoculation method, and relates to the technical field of biology. The separation and purification method comprises the following steps: taking infected chicken manure, adding water, and filtering; centrifuging, adding a saturated saline solution into the precipitate, centrifuging, and adding water into the supernate to obtain an oocyst diluent; centrifuging, and adding chloramine T solution into precipitate to obtain sporulated oocyst suspension; centrifuging, adding saturated saline solution into the precipitate for resuspending, centrifuging and collecting oocyst precipitate; adding a sodium hypochlorite solution for resuspension, and collecting oocysts; and shaking and crushing, stopping shaking when all sporangia escapes, centrifuging to obtain sporangia, adding pancreatin digestive juice for digestion, filtering, centrifuging, and collecting precipitate to obtain eimeria tenella sporozoites. According to the inoculation method, the sporangiums or sporozoites are accurately injected into the middle sections of muscular stomachs and small intestines of the chickens, and successful infection is achieved. The purification degree of the sporangiums and sporozoites obtained by the purification method is high, the experimental operation is simple and convenient, and the infection success rate after injection inoculation reaches 100%.

The invention discloses a wild-imitating gastrodia elata f. glauca and gastrodia elata f. elata cultivation method. The method comprises the steps as follows: (1) cross breeding: A selecting seeds; B cultivating gastrodia elata f. elata at the natural temperature, cultivating gastrodia elata f. glauca in a temperature-control chamber, and controlling the temperature at 25-28 DEG C 5-25 days before flowering of the gastrodia elata f. glauca; C performing topping 10 days before racemes of gastrodia elata grow to top ends; D after the gastrodia elata f. glauca and gastrodia elata f. elata flower simultaneously, performing reciprocal interchange artificial pollination; E picking capsules during cracking, uniformly mixing the powdery capsules with germinating strains; (2) bacterium bed preparation; (3) seed gastrodia elata dibbling; (4) harvesting. The gastrodia elata f. glauca and gastrodia elata f. elata hybridization rate is as high as 95% or above, a mild-imitating growth environment is created for growth of the gastrodia elata, and the first-generation strains have high activity, high reproductive capacity, high inoculation success rate, cannot be polluted by living contaminants easily, have heterosis, have high yield, can produce 750 kg of gastrodia elata f. glauca and gastrodia elata f. elata per mu on average and have high medicinal value and high disease resistance.

The invention discloses a wild-planting-simulated high-quality fungus bed cultivation method for rhizoma gastrodiae. The method includes the steps of firstly, digging holes in pollution-free original forest land, wherein each hole is 30-50cm in width, 50-70cm in length and 10-30cm in depth, and the bottom surface slope of each hole is 30 degrees; secondly, processing wood into main materials and auxiliary materials, transversely placing 2-6 main materials along the hole bottom surface slope direction, and placing the auxiliary materials around the main materials; thirdly, filling gaps between the placed main materials and auxiliary materials with fine soil; fourthly, inoculating bevel connections and adjacent bevel connection contact parts with armillaria mellea blocks cultured in advance; fifthly, spreading fragmentary leaves after the inoculation of the armillaria mellea; sixthly, removing surface soil, and covering the main materials and the auxiliary materials with fine soil. The method has the advantages that the contact area between the armillaria mellea and wood is large, first-generation strains are high in activity and fast in reproduction, and a cultivated fungus bed is high in inoculation success rate and cannot be easily polluted by contaminating microorganisms.

The invention relates to a novel method for breeding cordyceps militaris by utilizing silkworm larvae. The method comprises the steps of taking live matured silkworm as the host, vaccinating cordyceps militaris strains through an injection method, and promoting silkworm to sleep, and the vaccination attainment rate is improved; after the method is adopted, the inactivation rate of the silkworm larvae reaches 90% to 100%, and the outgrowth rate of fruiting body reaches 85% to 100%; moreover, the water-solubility protein, polysaccharide, cordycepic acid, cordycepin and adenosine of the cordyceps militaris bred by the method are higher than those of cordyceps militaris bred by silkworm chrysalis in content. The novel method for breeding cordyceps militaris by utilizing silkworm larvae has the advantages of convenience in operation, low breeding cost, good experiment repeatability and wide application prospect, and is suitable for large-scale cultivation.

The invention relates to a sterilization device used in shiitake mushroom industrial production. The device comprises a sterilization chamber. A first sealing door and a second sealing door are separately arranged at the two ends of the sterilization chamber. The second sealing door is directly positioned in an inoculation workshop. The sterilization chamber is separately provided with a steam inlet pipe and a steam discharge pipe. A gas distribution pump is arranged in the ground of the sterilization chamber. The outlet of the steam inlet pipe is positioned in the gas distribution channel. A plurality of gas distribution holes are formed in the top of the gas distribution channel. An exhaust valve is arranged on the steam discharge pipe. According to the invention, the second sealing door which serves as the outlet of the sterilization chamber is directly arranged in the inoculation workshop, such that fungus cylinders are prevented from being contaminated by hybrid fungi in air during a transport process after sterilization. Therefore, inoculation success rate is improved, and subsequent inoculation operation steps are reduced. With bottom gas distribution and sealed pressurization sterilization manners, and in combination with a post-stage active heat discharge manner, sterilization time is shortened, and production efficiency is improved. The device is suitable for shiitake mushroom industrial production.

The present invention discloses a hepatitis B vaccine vaccination prevention and control method of rural children, the method is characterized by comprising psychological intervention on an object of vaccination, sequence vaccination of hepatitis B vaccine, a return visit for monitoring after the implementation of the vaccination and interference processing of vaccination problems, hepatitis B vaccine vaccination epidemic prevention of children can be improved, scruples of the object of vaccination and a guardian can be eliminated, the success rate of the vaccination can be improved, and improvement of hepatitis B vaccine vaccination prevention and control of the rural children and the quality of service can be facilitated.

The invention belongs to the technical field of breeding, and discloses desert cistanche seed paper and a preparation method thereof. The preparation method comprises the following steps: preparing raw materials: cistanche seeds, yellow clay mud, rolled toilet paper, fresh swimming bladder, bone glue, suaeda glauca, flour, water, starch and newspaper; adding the yellow clay mud into the starch, grinding into powder, adding water for soaking and stirring into a paste, respectively making the suaeda glauca and the flour into a paste, cooking the swimming bladder and soil into fertile water and cooking the bone glue and the soil into glue, sequentially adding into the mud paste, and uniformly stirring; brushing the cut newspaper with the prepared mud paste by using a brush, spreading about 80cistanche seeds on the brushed newspaper, covering the newspaper with a layer of toilet paper with the same size, spreading out the seed paper one by one for drying in the shade, and bundling and bagging 100 pieces of paper in each bag. By the preparation method, the germination rate of the cistanche seeds is effectively increased, inoculation can be effectively performed, and the sowing amount is greatly reduced.

The invention discloses a Ustilago-maydis haploid strain UM02. The collection number of the Ustilago-maydis haploid strain UM02 is CGMCC No.15387. The Ustilago-maydis haploid strain UM02 is combined with a haploid strain matched with the Ustilago-maydis haploid strain UM02 in mating type, then the pathogenicity is high, the success rate of inoculation is high, an identification result of the ustilago maydis resistance of maize varieties is accurate and reliable, and the consistency is good. When the Ustilago-maydis haploid strain UM02 is used for maize mushroom production, the yield of maize mushrooms is high, husk leaves are wrapped with the Ustilago-maydis haploid strain UM02, and harvesting and transportation are convenient.

The invention belongs to the technical field of traditional Chinese medicine biology, and particularly relates to a culture method of liquid strain-solid cultivation species of straw rotting edible fungi. The culture method comprises the specific steps of liquid strain propagation, formula and preparation of the solid culture species of traditional Chinese medicine residues, fermentation of cultivation materials, greenhouse cultivation and the like. The culture method has the advantages that the resource utilization of the traditional Chinese medicine residues is combined with the liquid strain, the culture method is more suitable for the large-scale utilization of the traditional Chinese medicine residues, the fermentation cycle can be effectively shortened, the survival rate of the edible fungi is improved, and the utilization efficiency of the medicine residues is improved. A feasible method is provided for the resource utilization of the traditional Chinese medicine residues.

The invention discloses a transportation and inoculation pot of an edible mushroom liquid strain and is composed of a storage tank, an air filter inoculator and an air compressor. The storage tank is composed of a tank body, a tank cover and tank feet. The storage tank is provided with a liquid inlet, an air inlet, an exhaust circular tube, a discharge opening, a drainage opening and a strain output opening. The tank cover is provided with a handle ring, a pressure gauge, an exhaust valve and an observation hole with a lamp. A seal washer is disposed between the tank cover and the tank body and is fixed by screws; the air filter can filter various microbes; the air inlet end of the air filter is connected to an air supply end of the air compressor; an exhaust end of the air filter is connected to an interface of the air inlet of the storage tank; and the inoculator is a pressure spraying type inoculator and is connected to an interface of the strain output opening of the storage tank. By adopting the transportation and inoculation pot, the inoculation range of the liquid strain can be expanded largely; investment for liquid strain production equipment can be reduced greatly; a process flow for edible mushroom production of small-size edible mushroom production growers is changed; a cultivation period of the edible mushrooms is greatly shortened; and economic benefits is greatly increased.

The invention relates to the technical field of seedling raising of Chinese medicinal materials, in particular to a method for tissue culture and rapid propagation of calamus calamus. The stem tip of calamus calamus is used as a raw material for tissue culture and rapid propagation, and undergoes callus induction culture, differentiation and proliferation culture, rooting and strong seedlings Cultivation has realized the tissue culture seedling of Iris calamus, accelerated the cultivation of Iris calamus seedlings, met the seedling demand in the planting process of Iris calamus, and provided a feasible plan for preserving the germplasm resources of Iris calamus.

The invention relates to a method for multiplying plant pathogenic bacteria, in particular to a method for inoculating and multiplying Puccinia maize. The invention provides a method for inoculating and propagating Puccinia maize, comprising: spraying Tween-20 solution on diseased leaves, eluting spores on the diseased leaves into Tween-20 solution to prepare spore suspension , inoculated onto healthy corn seedlings, harvested and multiplied spores after cultivation, and used healthy corn seedlings for repeated transfer. The method has fast propagation speed and high efficiency of Puccinia multihelix, and can quickly obtain a large number of high-activity Puccinia multihelix spores, which greatly shortens the propagation period of Puccinia multihelix, and provides a great opportunity for the study of southern corn rust. Efficient strain propagation methods and technical support.