35 results about "Pinworm disease" patented technology

The invention discloses a method for planting celery. The method for planting the celery comprises the following steps of sowing, seedling cultivating, growing and harvesting. According to the method for planting the celery, the healthy growth of the celery is facilitated, the celery is prevented from being damaged by pests and diseases, the celery can grow rapidly, the quality is high, and the yield is high.

The invention discloses a traditional Chinese medicine for treating a roundworm disease in a biliary tract. The traditional Chinese medicine is characterized by comprising the following herbs in part by weight: 50 parts of dark plum, 25 parts of combretum indicum, 25 parts of chinaberry seed, 15 parts of fructus aurantii, 15 parts of pepper, 15 parts of torreya seed, 25 parts of areca nut, 15 parts of costus root and 5 parts of liquorice. According to characteristics of the roundworm disease in the biliary tract, the traditional Chinese medicine is formulated by a scientific method; the traditional Chinese medicine not only can kill roundworms, but also has the effects of circulating Qi and stopping pains, strengthening the spleen and promoting digestion, soothing the liver and benefiting the gallbladder, restoring normal functions of the spleen and the stomach, and eliminating complications caused by the roundworms in the biliary tract; in four years, among 200 patients taking the traditional Chinese medicine, the effective rate is 100% and the cure rate is 100%; with 8 hours after the traditional Chinese medicine is taken, the traditional Chinese medicine can take effect; and after 2-3-day continuous use, the roundworm disease in the biliary tract can be cured.

The invention discloses a control method for preventing leaf vegetables against root-knot nematode. The method comprises the following steps: dividing to-be-processed land into a land parcel without root-knot nematode, land parcels at moderately severe degree and incredibly serve degree of root-knot nematode and a land parcel with serious root-knot nematode according to damage degree of root-knot nematode; taking a preventative mode to the land parcel without root-knot nematode and controlling an infection source and cutting off a transmission mode to prevent occurrence of root-knot nematode; locally changing soil and simmering saw dust as for the land parcels at moderately severe degree and incredibly serve degree of root-knot nematode and preventing root-knot nematode in combination with small vegetable beds or ridging planting, drip irrigation, intercropping and interplanting techniques to reduce density of population; and closing a greenhouse as for the land parcel with serious root-knot nematode and preventing root-knot nematode in combination with biological bacterial fertilizers, small vegetable beds or ridging planting, drip irrigation, intercropping, different crop rotation techniques. The control method for preventing leaf vegetables against root-knot nematode is simple and practicable and low in cost so that non-pollution prevention and control over root-knot nematode for leaf vegetables is effectively achieved.

The invention relates to a composition for preventing and treating sporozoosis of fish. The composition is characterized by being prepared from the following raw materials in parts by weight: 6-10 parts of artemisia apiacea, 6-10 parts ofazedarach, 10-18 parts of scutellaria baicalensis, 8-12 parts of pumpkin seeds, 3-8 parts of cortex dictamni, 3-8 parts of cordate houttuynia, 10-18 parts of radix isatidis, 10-18 parts of perilla leaves and 6-10 parts of rhizoma phragmitis. The composition has a relatively good disease resistance, can be used for effectively preventing and treating sporozoosis of fish and reducing death of sporozoosis fish, does not have toxic or side effect or medicinal residues, and is relatively low in production cost and convenient to use.

The invention discloses a stem nematode disease resistance identification method for sweet potato related wild species, and belongs to disease resistance identification methods of related wild species of crops. The identification method comprises the steps that the sweet potato wild species to be identified are planted into flowerpots containing sterilized soil, and one seedling is planted in each flowerpot; two hundred stem nematode worms are inoculated to each wild seedling by the adoption of the root dripping method, and the seedlings are cultivated at 25 DEG C, 80 percent of humidity, and a photoperiod of 14 / 10(L / D); the seedlings are watered appropriately each day, and the humidity of the soil in the flowerpots is controlled to be within the range of 18 percent-25 percent; after the seedlings are cultivated for 40 days, the subterraneous stems of the wild species are dug out and are vertically split with dissecting scissors, the stem nematode disease damage areas in the stems are measured, disease indexes are divided according to the ratio of the damaged areas to the stems, and the resistance levels of the wild species are determined. The processing means of the identification method is scientific and effective, the operation process is simple, the experimental period is short, and pollution to the environment by the test materials can be avoided. Besides, test results can comprehensively embody the levels of resistance to the stem nematode disease of the sweet potato related wild species.

The invention relates to a Chinese herbal preparation for treating trichodiniasis of fishes. The Chinese herbal preparation is characterized by consisting of the following materials in percentage by weight: 5-25% of a radix sophorae flavescentis aqueous extract, 5-25% of a melia azedarach aqueous extract, 5-25% of a surfactant, 10-20% of a dissolving agent and the balance of a solvent, wherein the surfactant is Tween-20; the dissolving agent is dimethyl sulfoxide; the solvent is water. The Chinese herbal preparation disclosed by the invention overcomes the shortcomings of common basic medicines, and is simple and convenient to apply and is rapid and uniform to spread after being sprayed in a whole process; the Chinese herbal preparation is used for treating trichodiniasis of fishes and is applicable to rainy day as well. The Chinese herbal preparation is free from drug tolerance and drug resistance, and is free from toxin and residue as well as pollution to water quality.

The invention discloses a preparation for prevention and treatment of tobacco and tomato meloidogyne spp. and an application thereof. The preparation for prevention and treatment of tobacco and tomato meloidogyne spp. has an active ingredient of a fermentation liquid of a strain of bacillus amyloliquefaciens YN2010-KC CGMCC No.5722. The preparation not only is a microbial fertilizer which can significantly promote the growth of plants, but also is a biological pesticide and has the average relative control efficiency on tobacco meloidogyne spp. disease of up to 78.32%; the preparation has the control efficiency on the tomato meloidogyne spp. disease of up to 74.50% or more, and has remarkable prevention and treatment effect. The preparation has low production cost, an application method is simple and easy to implement, and the preparation can be used for direct seed dressing and root irrigation, has no toxicity, no pollution and no residue, and is safe to human and livestock.

The invention relates to a pure Chinese medicinal composition for treating the nematodosis of poultry. The pure Chinese medicinal composition is characterized in that the pure Chinese medicinal composition is prepared by mixing the following components in part by weight: 15 to 35 parts of pharbitis seed, 15 to 25 parts of almond, 25 to 45 parts of areca catechu, 10 to 20 parts of realgar, 15 to 35 parts of stone-like omphalia, 15 to 35 parts of rhizoma coptidis, 30 to 50 parts of hawthorn, 20 to 40 parts of white paeony root, 25 to 45 parts of dark plum fruit, 25 to 45 parts of sessile stemona root and 25 to 45 parts of Szechwan chinaberry fruit. The invention has the advantages that the pure Chinese medicinal composition is rational in formula, easy to prepare, free of toxic or side effects, medicament residues and medicament resistance and high in superfine grinding degree and bioavailability, can treat both symptoms and root causes and has remarkable curative effects, and clinical results prove that the pure Chinese medicinal composition has a special effect of treating the infectious bursal disease of the poultry.

The invention relates to a pure Chinese medicinal composition for treating the nematodosis and taeniasis of fish and a preparation method of the pure Chinese medicinal composition. The pure Chinese medicinal composition for treating the nematodosis and taeniasis of the fish is prepared by mixing the following components in part by weight: 25 to 45 parts of quisqualis indica, 25 to 45 parts of rhubarb, 20 to 40 parts of salt, 20 to 40 parts of fineleaf schizonepeta herb, 15 to 35 parts of caulis perillae, 15 to 35 parts of chinaberry bark and root-bark, 20 to 40 parts of costus root, 20 to 40 parts of dandelion, 25 to 45 parts of cyrtomium rhizome and 25 to 45 parts of areca catechu. The pure Chinese medicinal composition and the preparation method have the advantages that the pure Chinese medicinal composition is rational in formula, easy to prepare, free of toxic or side effects, medicament residues and medicament resistance and high in superfine grinding degree and bioavailability, can treat both symptoms and root causes and has remarkable curative effects, and clinical results prove that the pure Chinese medicinal composition has a special effect of treating the nematodosis and taeniasis of the fish.

The invention belongs to the technical field of plant root-knot nematode inoculation methods, and particularly provides a plant root-knot nematode potting three-layer sick soil inoculation method. Themethod comprises the steps of 1, disease-free soil collection and treatment; 2, sick soil collection and treatment; 3, sick soil expanding propagation cultivation; 4, expanded propagation sick soil pretreatment; and 5, three-layer sick soil inoculation. The plant root-knot nematode potting three-layer sick soil inoculation method adopts a three-layer sick soil inoculation technology that disease-free soil is inoculated on upper and lower layers, and nematode sick soil is inoculated on a middle layer. Compared with an existing root-knot nematode egg and larvae inoculation technology, the technology approaches natural inoculation, the inoculation survival rate is high, a nematode selecting link is omitted, and the workload is greatly reduced. Compared with an existing root-knot nematode single sick soil inoculation technology, the technology has the advantages that the usage amount of the sick soil is reduced by 1 / 2-2 / 3, and the workload and cost of the collection, transportation, expanding propagation cultivation and the like of the sick soil are greatly reduced; and newly transplanted seedlings can be protected from being infected by nematodes, not only can the success of the inoculation be ensured, but also too early disease death of the seedlings can be avoided.

The invention relates to a biocontrol strain SFC-3 for controlling nematode disease and application thereof, which can effectively solve that problem of unstable control effect and high use cost of existing biocontrol strain for controlling root-knot nematode diseases, and is classify and named as Trichoderma asperellum (CGMCC NO. 16097), which is a biocontrol strain SFC-3. At the concentration of1 * 109 CFU / mL, the egg hatching and the second instar larvae of root-knot nematode are inhibited. The strain can remarkably reduce the invasion of nematodes, and the control effect on root-knot nematode diseases is 78.51%. The strain can be used for irrigating roots when vegetable seedlings are transplanted, and has the growth promoting effect.

The invention relates to a biological intelligence fungus block for preventing and treating stubby root nematodes. The biological intelligence fungus block is prepared by fermenting and drying three bacterial strains: ACCC11084: bacillus megaterium bacillus megaterium de Bary, ACCC10632: bacillus subtilis (Ehrnberg) Cohn and ACCC32001: paecilomyces lilacinus (Thom.)Samson to obtain bacterial strain mother powder, and then mixing and pressing with carrier auxiliary materials at the ratio. The intelligence fungus block can be used for growing seedlings of multiple vegetables, flowers and economic crops, is capable of preventing nematodes from damaging plants during the seedling stage, still can play good prevention and treatment roles on the stubby root nematodes after being transplanted into a field or a greenhouse, and especially has a good control effect on soybean cyst nematodes, cucumber root knot nematodes and peanut root knot nematodes.

The present invention relates to application of an Angiostrongylus Cantonensis protein PAS-5, which is an application of the immune enhancement effect of the Angiostrongylus Cantonensisprotein PAS-5, especially application of the Angiostrongylus Cantonensisprotein PAS-5 in the preparation of immune enhancement drugs. According to the invention, a theoretical basis is provided for the treatment of Angiostrongylus Cantonensis disease, and a potential target is provided for the research of the therapeutic drugs. At the same time, the foundation is laid for the research and development of the drugs for the treatment of Angiostrongylus cantonensis disease, a target is provided for the treatment of Angiostrongylus Cantonensis, and considerable market prospects and applicability are achieved.

The invention discloses a Chinese herbal medicine and poison bait for controlling fish trichodiniasis and a preparation method thereof. The Chinese herbal medicine is prepared from, by weight, 21-30 parts of cyrtomium rhizomes, 27-45 parts of radix sophorae flavescentis, 30-60 parts of red-knees herbs, 24-48 parts of chinaberry leaves, 20-25 parts of insecticyanin, 20-30 parts of ginger and 27-48 parts of allicin. Accordingly, by mixing and processing pure natural Chinese herbal medicine, feed, glycine betaine, blood powder and flour, the poison bait is prepared and matched for use with the Chinese herbal medicine which are not processed into the poison bait to control the trichodiniasis. The Chinese herbal medicine and poison bait are high in safety and can be used for production of various kinds of fries, pollution to water quality does not exist, the defect that insecticide cannot be used or is used with caution in the fry stage is well overcome, no drug residue exists, insecticide resistance cannot be caused to aquatic products, subsequent finished fish product culturing is promoted, and a wide application prospect is achieved.

The invention discloses an application of miR-101b-3p in diagnosis and adjuvant treatment of angiostrongylus cantonensis. The application claims the use of miR-101b-3p or its mimetic or its activatoras a marker or a target gene for gene therapy or drug screening of angiostrongylus cantonensis. According to the invention, miR-101b-3p is involved in the regulation of differential expression of AcSOD3 protein in rats and mice, so that the resistance of the worm-against-host derived reactive oxygen can be regulated, the different antioxidant capacities of the worms in suitable and unsuitable hosts can be regulated, and the parasitic adaptation in suitable and unsuitable hosts can be regulated. Therefore, the expression level of miR-101b-3p can be an important diagnostic indicator and a therapeutic target for the diagnosis of unsuitable host infected with angiostrongylus cantonensis, the application reveals a key mechanism for the survival of angiostrongylus cantonensis in the final host,and provides a new idea for improving and optimizing the existing strategies for prevention and control of angiostrongylus cantonensis.

The invention discloses a method for planting a potted plum tree. The method for planting the potted plum tree comprises the following steps of soil preparation, field planting, growing and harvesting. The method for planting the potted plum tree has the advantages that healthy growth of the plum tree is facilitated, the plum tree is prevented from being damaged by pests and diseases, the quality is high, the yield is high, and fruits are fragrant, sweet and tasty.

The invention discloses a method for controlling a blended tobacco root knot nematode disease.The method comprises the steps that before tobacco seedlings are transplanted, a root knot nematode disease control agent abamectin is applied into a transplant field tobacco pond, after transplanting is conducted and then watering is finished, a root knot nematode disease control agent abamectin and carbosulfan; root-inducing wettable powder is diluted with water by 150-250 times, and 150 ml-250 ml of diluted root-inducing wettable powder is applied for root irrigation per pond 7-10 days after the tobacco seedlings are transplanted; after the tobacco seedlings are transplanted, it is guaranteed that the moisture content of soil rhizosphere ranges from 10% to 20%.According to the method for controlling the blended tobacco root knot nematode disease, the two agents are subjected to reasonable matching and alternative using for tobacco regions such as Stone Forest, Yiliang, Anning, Jinning and Xundian where various nematodes occur in a hybrid mode, wherein when transplanting is conducted, abamectin is adopted to mix with soil or sand, pesticide application and earthing are conducted near the roots; after the transplanting is finished, 'Jindongwang' is used for root irrigation on the same day, and 'Jindongwang' can be adopted to conduct root irrigation twice for heavy disease areas.

The invention discloses a method for preventing and treating the tobacco root knot nematode disease. The method comprises the first step of agricultural prevention and control, the second step of physical prevention and control and the third step of chemical prevention and control. The step of agricultural prevention and control includes the substeps that firstly, tobacco and graminaceous crop rotation is conducted for 3-5 years; secondly, plant ash and quicklime are used in the ratio of (3-4):1, 150-250 kg of the mixture is applied to each mu of field and scattered before deep ploughing and winter baking, and deep ploughing and winter baking are completed before winter to reduce overwintering eggs; thirdly, an organic fertilizer is applied, 30-50 kg of compost cake fertilizer and 150-250 kg of compost agricultural fertilizer are additionally applied to each mu of field, and then deep ploughing is conducted to improve soil fertility. A prevention means is used, and the plant ash and the quicklime are used for acidification and are combined with physical prevention and control and chemical prevention and control. The tobacco root knot nematode disease can be effectively prevented, the tobacco yield is increased, the tobacco grade is improved, and good economic benefits are obtained.

The invention discloses a traditional Chinese medicine composition for preventing and treating fish dactylogyrosis. The traditional Chinese medicine composition contains active ingredients, and the active ingredients comprise radix zanthoxyli extract, fructus evodiae extract, ginkgo biloba sarcotesta extract and macleaya cordata extract. The traditional Chinese medicine composition has the beneficial effects that the selected Chinese herbal medicine components accord with the specifications of The Drug Administration Law of the PRC, dactylogyrus can be killed in a relatively short period of time by using combined action of all the traditional Chinese medicine, the disease symptoms of fish are alleviated, the survival rate is improved, and the traditional Chinese medicine composition is completely harmless for human bodies.

The invention discloses a method for preventing solar greenhouse root-knot nematode disease. The method comprises the steps of replacing an old greenhouse membrane with a new greenhouse membrane in a greenhouse-resting period during a high-temperature season, conducting deep ploughing on soil in the greenhouse for 30-50 cm and afterwards conducting levelling, and forming multiple parallel water-conducting ditches on the soil; using plastic mulching film to cover the soil in the greenhouse, irrigating the soil with sufficient water from water-irrigating holes of the water-conducting ditches , and afterwards conducting smothering in the sealed greenhouse, wherein in the smothering period, the greenhouse is covered with a greenhouse mat from every dawn to next morning, and the greenhouse mat is drawn back from the morning to the dawn; conducting continuous smothering for 15 d-25 d, then opening a wind opening of the greenhouse and conducting ventilation for 5-7 days, and uncovering the mulching film so that sowing can be conducted. By means of the method for preventing the solar greenhouse root-knot nematode disease, the occurrence of danger of root-knot nematode can be effectively controlled, the safe production of greenhouse vegetables is guaranteed, and the problem of inferior quality of agricultural products caused by excessive use of chemical pesticides is solved.

The invention discloses a compound traditional Chinese medicine for preventing fish dactylogyrosis. The compound traditional Chinese medicine is mainly prepared from cortex ailanthi, fructus liquidambaris and pumpkin seeds; cortex ailanthi can effectively kill dactylogyrus and worm eggs in the early stage of fish dactylogyrosis prevention; fructus liquidambaris has a significant killing effect on thedactylogyrus and worm eggs in the early and later stages of fish dactylogyrosis prevention; the pumpkin seeds have the effect of removing toxins and destroying intestinal worms, mainly acts on the later stage of fish dactylogyrosis prevention and is used for killing a small amount of remaining dactylogyrus and worm eggs; the compound traditional Chinese medicine prepared from cortex ailanthi, fructus liquidambaris and the pumpkin seeds can give consideration to the whole prevention process of fish dactylogyrosis; the compound traditional Chinese medicine for preventing fish dactylogyrosis can effectively prevent fish dactylogyrosis, improve the survival rate of breeding, solve the problem that the dactylogyrus easily generate resistance to a single drug and inhibit the metabolism and growth of harmful microorganisms in aquaculture water to a certain extent, and therefore the quality of aquaculture water is improved.

The invention relates to a pure Chinese medicinal composition for treating the nematodosis and taeniasis of fish and a preparation method of the pure Chinese medicinal composition. The pure Chinese medicinal composition for treating the nematodosis and taeniasis of the fish is prepared by mixing the following components in part by weight: 25 to 45 parts of quisqualis indica, 25 to 45 parts of rhubarb, 20 to 40 parts of salt, 20 to 40 parts of fineleaf schizonepeta herb, 15 to 35 parts of caulis perillae, 15 to 35 parts of chinaberry bark and root-bark, 20 to 40 parts of costus root, 20 to 40 parts of dandelion, 25 to 45 parts of cyrtomium rhizome and 25 to 45 parts of areca catechu. The pure Chinese medicinal composition and the preparation method have the advantages that the pure Chinese medicinal composition is rational in formula, easy to prepare, free of toxic or side effects, medicament residues and medicament resistance and high in superfine grinding degree and bioavailability, can treat both symptoms and root causes and has remarkable curative effects, and clinical results prove that the pure Chinese medicinal composition has a special effect of treating the nematodosis and taeniasis of the fish.

The invention relates to traditional Chinese medicinal granules for preventing and controlling pig fasciolopsiasis. The traditional Chinese medicinal granules comprise grand torreya seeds, pharbitis seeds and common carpesium fruits. Finished products are prepared by weighing each component according to a proportion, adding water for decoction twice, mixing filtrate, performing concentration, drying and crushing, adding sucrose, and performing granulation, sieving, drying and sieving. The traditional Chinese medicinal granules are mixed with water according to a certain proportion when being used, empty diseased pigs are fed with the traditional Chinese medicinal granules, and after the traditional Chinese medicinal granules are continuously used for 3 to 5 days, the weights of the diseased pigs are remarkably increased, abdominal distension disappears, and fresh manure is collected and detected not to contain any ovum by a washing precipitation method; compared with the conventional method, the traditional Chinese medicinal granules have the advantages that parasites can be effectively killed, the diseased pigs can be rapidly restored, and medicinal residues and toxic or side effects are avoided.

The invention discloses a preparation method of a Tibetan medicine for treating echinococcosis. The Tibetan medicine is prepared from active components of embelia laeta, seeds of butea monosperma and semen hyoscyami with a mixed grinding method, a water extraction and alcohol precipitation method or an alcohol extraction and water precipitation method and can be prepared in any common oral dosage form. The Tibetan medicine is broad-spectrum vermifuge, can be used for treating nematodosis such as a hookworm disease, a roundworm disease, a whipworm disease, a pinworm disease, a trichinella spiralis disease and the like and can be also used for treating a cysticercus disease and echinococcosis.

The invention relates to a composition for preventing and treating sporidiosis of fishes. The composition is characterized in that the composition is prepared from the following raw materials in parts by weight: 3 to 6 parts of radix sophorae flavescentis, 4 to 7 parts of cortex meliae, 8 to 12 parts of flower of winter jasmine, 3 to 6 parts of peach twig, 6 to 10 parts of herba oxalidis corniculatae, 10 to 15 parts of radix isatidis, 6 to 10 parts of caulis polygoni multiflora and 8 to 12 parts of spica ptowrunellae. The composition disclosed by the invention has the advantages of better disease resisting effect, no toxic and / or side effects or drug residue, low production cost and convenience in use; the sporidiosis of the fishes can be effectively prevented and treated, and the death of the fishes due to the sporidiosis of the fishes is reduced.

The invention provides disease preventing compost for balcony vegetables and a preparation method of the disease preventing compost. In the formula, bead tree bark powder is adopted, toosedarin antifeedant included in bead trees is high in activity and within antifeeding spectrum, a certain amount of toosedarin can effectively prevent and control more than 40 kinds of important plant pests, is systemic, and can be absorbed by roots to be conducted to the whole body, and a whole plant can resistant to the pests. The rich toosedarin in the bead trees has a very good preventing and control effectcommon aphids, greenhouse whitefly, red spiders, cabbage caterpillar, grubs and other insect pests and also has a good preventing and control effect on vegetable root diseases, such as wilt and rootknot nematode diseases, caused by parasitic nematodes, fungi, bacteria and viruses in soil. The method is used for preparing the compost, and an effective pest preventing effect is achieved. Waste edible mushrooms sticks, wood vinegar, vermiculite, fume carbon, akadama soil, slow release fertilizer and a soil conditioner are used in cooperation, and the effect is more remarkable.

A new technology for the prevention and control of wheat grain nematode disease, which is characterized by strengthening inspection, preventing long-distance spread of seeds with galls, removing galls in wheat seeds, implementing crop rotation for more than 3 years to prevent galls from being mixed into manure, and applying fully decomposed organic fertilizers. Fertilizer, chemical treatment of seeds, chemical control and other processes, it solves the problem of reduced yield per mu of wheat due to nematode disease. It is extremely suitable for large-scale wheat farmers to increase the yield per unit area.

The invention discloses a hybridoma cell line ACV1 and a monoclonal antibody ACV McAb for producing angiostrongylus cantonensis V-stage larvae 55KD antigen. The antibody and the angiostrongylus cantonensis V-stage larvae 55KD antigen can be in specific binding. The preparation method of the antibody is simple, and the antibody can be obtained by direct secretion of the hybridoma cell line ACV1. The antibody has higher binding specificity and affinity with the angiostrongylus cantonensis V-stage larvae 55KD antigen, and the monoclonal antibody can be applied to detect the angiostrongylus cantonensis circulating antigen levels, thereby providing a method of early diagnosis of the angiostrongylus cantonensis.

The invention relates to a soil nematode preventing and controlling method. According to the soil nematode preventing and controlling method, after panax notoginseng is infected by nematodiasis during a planting process, 0.5-1 L of a pyrethrin solution with a mass percentage concentration of 30% and 0.2 L of an abamectin microemulsion with a mass percentage concentration of 1.8% are added to per 1000 L of water to prepare a nematode removing solution, the nematode removing solution is applied onto the panax notoginseng suffering the panax notogiseng root-knot nematode disease, per mu is applied with 2000-3000 L of the nematode removing solution, the applying is performed every 7-10 days, and the applying is performed 2-3 times. According to the present invention, the problems that the soil having the nematode must be avoided before the planting and the panax notogiseng root-knot nematode disease cannot be effectively treated after the development of the panax notogiseng root-knot nematode disease in the prior art are solved.

The invention relates to a fluorescent quantitative PCR (Polymerase Chain Reaction) primer, probe and kit for detecting dirofilaria immitis. The sequences of the primer are shown as SEQ ID NO.1 and SEQ ID NO.2, and the sequences of the probe are shown as SEQ ID NO.3 and SEQ ID NO.4. The primer and the probe can be adopted for detecting dirofilaria immitis through an FRET (fluorescence resonance energy transfer)-PCR technology, and can specifically amplify the nucleic acid of a unique pathogen dirofilaria immitis causing the dirofilaria immitis disease, without the amplification of nucleic acid of other similar filaria which can not cause dirofilaria immitis disease.