387 results about "Macleaya cordata" patented technology

The invention relates to an oligochitosan and plant source substance-containing agricultural composition and applications, wherein the plant source substance is prepared by one or more of derris, tripterygium glycosides, tobacco, pyrethrum cinerariifolium, tagetes erecta, eupatorium adenophorum, radix stemonae, cayenne pepper, macleaya cordata, radix sophorae flavescentis, pyrethrum cinerariifolium, chinaberry, excoecaria sebifera, black nightshade herb, rhododendron sinensis sweet, coriaria sinica maxim, garlic, fruit of cubeb litsea tree, yellow flowered rhododendron, thymelaea, radix euphorbiae lantu, lysimachia foenum-graecum hance, peppermint, calamendiol, cortex cinnamomi, black pepper, artemisia scoparia, rhizoma alpiniae officinarum, mugwort, pepper, cynanchum komarovii, chinese honeylocust abnormal fruit, stramonium, ginko, camphora officinarum, caulis spatholobi, chinese mosla herb, herba asari, red sandalwood, erythrina indica, myrsine africana and fructus cnidii. The composition is low in people and livestock and non-target organism toxicity, fast degraded in the environment, low in residue, and free from environmental pollution. The oligochitosan and certain plant source substances in the composition are mixed and can achieve synergistic interaction, and the usage amount of the plant source substances can be reduced.

The invention belongs to the field of plant protection, and relates to an agricultural composition containing antibiotics and plant source materials, wherein antibiotic is one or several selected from abamectin, ivermectin, milbemectin, pleocidin, nikkomycin, liuyangmycin, streptomycin, polyoxins, chunleimeisu, jinggangmeisu, ningnanmycin and the like; the plant source material is one or several of smashing material, crude extract, or active ingredient of derris, thunder god vine, tobacco, pyrethrum, tagetes, crofton weed, radix stemonae, cayenne, macleaya cordata, radix sophorae flavescentis and the like. The composition provided by the invention expresses well synergism in a certain matching range, and has excellent control efficiency for crop diseases and pests.

The invention discloses a plant efficient drip irrigation fertilizer, which comprises the following components in percentage by mass: 5 to 30 percent of major elements, 1 to 10 percent of trace elements, 0.1 to 2 percent of vitamin, 0.001 to 1 percent of amino acid, 0.1 to 6 percent of humic acid, 0.001 to 1 percent of macleaya cordata total alkali and the balance of deionized water. The plant efficient drip irrigation fertilizer overcomes the defects that the conventional drip irrigation fertilizer is single in nutritional ingredients, contains harmful substances and blocks drip irrigation equipment easily, and has the advantages of comprehensive nutrition, no toxicity, high dissolvability, no pipeline blockage and production cost saving.

The invention relates to a Chinese herbal medicinal bactericide, which aims to solve the problem of public hazards of the conventional bactericide. The bactericide comprises the following Chinese herbal medicines in part by weight: 40 to 120 parts of sessile stemona root, 40 to 120 parts of camphor tree, 35 to 105 parts of pomegranate rind, 35 to 105 parts of macleaya cordata, 35 to 105 parts of common andrographis herb, 35 to 105 parts of isatis root, 30 to 90 parts of barberry root, 30 to 90 parts of vietnamese sophora root, 30 to 90 parts of common fibraurea stem, 30 to 90 parts of densefruit pittany root-bark, 30 to 90 parts of officinal magnolia bark, 30 to 90 parts of common threewingnut root, 25 to 75 parts of akebia stem, 25 to 75 parts of oriental variegated coralbean bark, 25 to75 parts of fourstamen stephania root and 25 to 75 parts of humifuse euphorbia herb. The bactericide is prepared by the following steps of: performing ethanol precipitation on the Chinese herbal medicines to obtain the original liquid medicine; diluting sodium usnic acid by using a solvent; and adding 0.1 to 0.3 weight percent of diluted sodium usnic acid into the original liquid medicine. The finished Chinese herbal medicinal bactericide comprises 0.3 percent of stemonine, is brownish red, has the pH value of 7 to 8 and the density of 1.10g / cm<3>, and has the advantages of capability of effective sterilization and no damage to humans.

The invention relates to a Chinese herbal medicinal insecticide, and aims to solve the problem of public hazards of the conventional insecticide. The Chinese herbal medicinal insecticide comprises the following Chinese herbal medicines in part by weight: 50 to 110 parts of macleaya cordata, 50 to 110 parts of eucalyptus robusta smith, 40 to 100 parts of betel nut, 40 to 100 parts of pumpkin seed,40 to 100 parts of clematis root, 40 to 100 parts of celastrus angulatus, 30 to 90 parts of stone-like omphalia, 30 to 90 parts of grand torreya seed, 30 to 90 parts of camphor tree, 30 to 90 parts of pomegranate rind, 30 to 90 parts of lightyellow sophora root, 30 to 90 parts of rhododendron molle, 30 to 70 parts of fourstamen stephania root, 30 to 70 parts of sessile stemona root, 30 to 70 parts of hindu datura flower and 30 to 70 parts of liquoric root. The Chinese herbal medicinal insecticide is prepared by the following steps of: performing alcohol precipitation on the Chinese herbal medicines to obtain the original liquid medicine; diluting sodium usnic acid by using a solvent; and adding 0.1 to 0.3 weight percent of diluted sodium usnic acid into the original liquid medicine. The finished Chinese herbal medicinal insecticide comprises 0.5 percent (g / ml) of sanguinarine, is brownish red, has the ph value of 7 to 8 and the density of 1.10g / cm<3>, and has the advantages of effective insecticidal capability, no damage to humans, high adsorbability and durable seepage force.

The invention provides a method for restoring uranium milltailings by utilizing plants, which is a technique for restoring the uranium milltailings by utilizing uranium-enriched plants (macleaya cordata) as the material. The method directly plants macleaya cordata into the uranium milltailings without other supplementary means and comprises the following steps: (1) sterilizing seeds; (2) plantingseedlings in pots; (3) transplanting the plants into the uranium milltailings; (4) and carrying out the centralized processing on the reaped overground part. The method solves the problem that the prior uranium milltailings release uranium and heavy metals under natural conditions to damage the environment. The method has the advantages of simple operation and management, high restoration rate aswell as low cost and environmental risk and beautifies the environment. The method is suitable for treating solid uranium waste storage yards in uranium mines, hydrometallurgy plants, various ettle and milltailing warehouses, and the like.

The invention discloses a phyto-pesticide and a use method thereof. The pesticide is used for controlling aphids, rice planthoppers and cabbage worms. A principle of 'monarch, minister, adjuvant and messenger' for drug formula in philosophy of the traditional Chinese medicament is used in the development of a product of the phyto-pesticide formula. The phyto-pesticide comprises four plant extracts including 0.5 to 10 percent by sanguinarine of macleaya cordata, which plays a main insect killing role and serves as a monarch drug; 2 to 20 percent by nicotinamide of tobacco extract, which is used for improving the insect killing effect of the 'monarch drug' and serves as a 'minister drug'; 5 to 10 percent by teasaponin of tea oil cake, which is used for making a target insect refuse to have food, generates certain gastrointestinal toxicity to reduce the viability of the insect and serves as an 'adjuvant drug'; 0.1 to 0.5 percent by natural borneol(L(-)-Borneol ) of borneol camphor, which can expel the insect and serves as a 'messenger drug'. The formula combines the 'monarch, minister, adjuvant and messenger' composite formula and an additive and a carrier to prepare the pesticide with insect expelling effect. The phyto-pesticide is effective, free from residue and environmentally-friendly.

The invention relates to a preparation method of macleaya cordata extracts, which comprises the following steps: carrying out diacolation on macleaya cordata medical materials by an acid solution; adding alkali into diacolation liquid for regulating the solution to the alkaline solution for carrying out alkali deposition; dissolving precipitates by low-carbon alcohol; adding a proper amount of active carbon for back flowing extraction; adding acid for generating salt after the recovery of partial solvents from extraction liquid, and separating out orange crystalline precipitates; filtering the precipitates; using alcohol to wash remained acid; and obtaining the macleaya cordata extracts after drying. The extracts have the main ingredients of benzophen anthridine alkaloid salt with sanguinarine and chelerythrine as the representatives, and the total content is not lower than 60 percent through being metered by raw alkali, wherein the content of the sanguinarine is not lower than 40 percent, and the content of the chelerythrine is not lower than 18 percent. The preparation of the macleaya cordata extracts of the invention has the advantages of simple method and equipment, stable process, short production period and little environment pollution. The obtained extracts have high yield, low cost and stable product quality.

The invention relates to a Chinese herb biological disinfectant to solve the public nuisance problem of the traditional disinfectant. The Chinese herb biological disinfectant is prepared by adopting the steps of preparing primary liquid medicine by ethanol precipitation of the following bulk drugs in weight ratio: common andrographis herb 40-120, santonin 40-120, celastrus angulatus 35-105, camphor tree 35-105, stephania tetrandra 35-105, rhododendron molle 35-105, macleaya cordata 30-90, pomegranate rind 30-90, clematis root 30-90, radix stemonae 30-90, eucalyptus robusta 30-90, sophora flavescens 30-90, cortex dictamni 25-75, Chinaberry bark and root-bark 25-75, rhizoma corydalis 25-75 and liquorice root 25-75; and adding sodium usnic acid with weight ratio of 0.01-0.03% in the primary liquid medicine, wherein the sodium usnic acid is dissolved with a solvent before the sodium usnic acid is added in the primary liquid medicine. The Chinese herb biological disinfectant contains 0.2% andrographolide, with color being brownish red, ph being 7-8 and density being 1.10g / cm<3>. The Chinese herb biological disinfectant has the advantages of effective disinfection and no harm to human.

The invention relates to the application of a macleaya cordata extractive in veterinary drugs for economic animals, in particular to the application of the macleaya cordata extractive in the preparation of veterinary drugs with favorable inhibition effect and killing effect on common livestock and poultry diseases, such as protoblem listeria monocytogenes, haemophilus and pasteurella.

The invention belongs to the field of microbial fermentation technology, and especially discloses a preparation method of a fermented Chinese herbal medicine lactagogue fodder for sows in lactation period, wherein the fermented Chinese herbal medicine lactagogue fodder is rich in Chinese herbal medicine active substances, active small peptides and probiotics; is high in nutrition value, and excellent in palatability; is easy for digestion; comprises less antinutritional factors; and is capable of promoting lactation with excellent effect. The fermented Chinese herbal medicine lactagogue fodder is prepared by fermenting a mixture of haw, macleaya cordata, medicated leaven, licorice, malt, motherwort, cowherb seed, corn flour and molasses by probiotics. The preparation method is capable of eliminating antinutritional factors, and the fermented Chinese herbal medicine lactagogue fodder is capable of increasing feed intake and lactation yield of sows in lactation period. The fermented fodder for sows in lactation period is prepared by drying after fermentation; is rich in Chinese herbal medicine active substances, active small peptides, lactic acid and probiotics; contains abundant and balanced amino acids; is high in nutrition value, and excellent in palatability; is easy for digestion; comprises less antinutritional factors; and is capable of promoting lactation of sows in lactation period with excellent effect.

The invention discloses a plant powder pesticide and a method for preparing the same. The plant powder pesticide comprises the following plant raw materials in weight portion: 200 to 300 weight portions of macleaya cordata, 300 to 400 weight portions of euphorbia humifusa, 200 to 300 weight portions of polygonum spicy, 50 to 150 weight portions of licorice powder, 300 to 400 weight portions of anisodus mairei, 150 to 250 weight portions of cortex pseudolaricis, 200 to 300 weight portions of cortex moutan, 150 to 250 weight portions of agrimony, and 150 to 250 weight portions of lycoris radiata. The method is relatively simple to operate, is carried out at normal temperature and pressure, and consumes low energy; and various plant raw materials can be combined together to be extracted most properly at the normal temperature, and mutual influences of the components are smaller. The plant powder pesticide has better killing effect on rice leaf insects, cabbage caterpillars, and aphids. The antifeedant rates to pests are more than 96 percent, and the mortality rate is more than 80 percent. The plant powder pesticide adopts a compound preparation of various plant components so that pests are not easy to generate drug resistance generally.

A veterinary drug for treating livestock dysentery comprises crude drug components in parts by weight as follows: 50-60 parts of macleaya cordata, 60-80 parts of red-knees herbs, 40-50 parts of herba andrographitis, 40-50 parts of houttuynia cordata, 5-10 parts of scutellaria baicalensis, 10-20 parts of honeysuckle, 10-20 parts of fructus forsythiae, 15-30 parts of portulaca oleracea, 15-20 parts of Chinese pulsatilla roots, 20-30 parts of coptis chinensis, 20-30 parts of ash bark, 15-25 parts of elecampane, 5-10 parts of liquorice and 10-20 parts of hydroxypropyl methylcellulose. Extracts of the crude drug components are prepared respectively, screened and fully mixed so as to prepare the veterinary drug. The veterinary drug is wide in application range, free from drug residues, scientific and concise in technological process, stable in product quality, applicable to industrial production and convenient to package, transport and carry the product.

The invention discloses an environment-friendly biological Chinese herbal medicine pesticide, which is characterized by comprising the following components by weight: 10-15 parts of Gelsemium elegans Benth, 5-9 parts of Chinese azalea flower, 5-9 parts of macleaya cordata, 7-10 parts of tobacco stem, 10-15 parts of gynura segetum, 2-6 parts of cortex meliae, 10-20 parts of hot grass leaves, 8-10 parts of nux vomica, 8-10 parts of sasangua cake, 2-9 parts of Anemone Vitifolia, 2-9 parts of dalbergia hupeana, 1-10 parts of a synergistic agent, 5-20 parts of dandelion, 4-10 parts of purslane, 6-9 parts of cocklebur fruit, 10-15 parts of pomegranate bark, 11-15 parts of folium artemisiae argyi, 12-18 parts of cordate houttuynia, 5-18 parts of Andrographis paniculata and 100-150 parts of water. The product of the invention has insecticidal and bactericidal effects, and can prevent pest and disease infestation; at the same time, the product provided by the invention has no harm to human and animals, no side effect and no pollution to the environment, and is safe to use.

The invention discloses a soilless culture substrate and application thereof. The soilless culture substrate is prepared by grinding and fully mixing the following components in percentage by weight:35 to 45 percent of fern, 25 to 35 percent of oil-tea camellia seed shell, 8 to 15 percent of melia azedarach plant, 5 to 10 percent of rape defatted cake, 5 to 15 percent of bloodroot and 3 to 8 percent of phosphate fertilizer. The soilless culture substrate is mainly applied to flower culture. The substrate is loose and breathable; the oil-tea camellia seed shell can kill earthworm and cutworm;the melia azedarach plant such as melia azedarach seed and the bloodroot such as macleaya cordata can kill plant diseases and insect pests such as aphid and tenia; and the phosphate fertilizer is mainly a calcium magnesium phosphate fertilizer commonly applied to crops to make plants and flowers grow better.

The invention belongs to the field of feeds, and in particular to a macleaya cordata extract-containing laying hen feed additive and application thereof, and the macleaya cordata extract-containing laying hen feed additive comprises the following components by weight: compound trace elements, compound vitamins, lysine, methionine, betaine, calcium hydrophosphate, stone powder, sodium chloride, sodium bicarbonate, probiotics, phytase, antioxidants, macleaya cordata extract, zeolite powder, rice husk powder and medical stone. The macleaya cordata extract-containing laying hen feed additive meets the need of nutrients of egg producing period of laying hens, can play the natural antibacterial and anti-inflammatory effects of the macleaya cordata extract, protect intestinal health, improve liver function, improve the body immunity, promote intestinal microbial degradation of essential amino acids and improve feed digestion and absorption rate so as to improve egg laying rate, maintain the peak time and ultimately improve the economic benefit of farmers.

The invention discloses flavoprotein oxidase genes of macleaya cordata in synthesis of sanguinarine and chelerythrine and application of the flavoprotein oxidase genes. Three flavoprotein oxidase genes joining in synthesis of sanguinarine and chelerythrine are found in a macleaya cordata genome for the first time, including genes Mc20113, Mc6407 and Mc6408. Steps of reaction are respectively verified by using a brewer's yeast system through upstream precursor feeding, and synthesis of sanguinarine and chelerythrine and midbodies can be achieved. The conversion efficiency of same functional genes of macleaya cordata and opium poppy is further compared by using a brewer's yeast heterologous expression system, and the result shows that the conversion rate of the gene of macleaya cordata is remarkably higher than that of opium poppy. The invention further discloses a molecular mechanism of synthesis of sanguinarine in macleaya cordata, and a theoretic basis and molecular assistant breeding targets are provided for breeding of macleaya cordata with high contents of sanguinarine and chelerythrine; and meanwhile, precious experience is provided for in-vitro artificial synthesis of sanguinarine and chelerythrine.

The invention discloses a macleaya cordata-scutellaria baicalensis composition for veterinary drugs or drug feed additives and an application of the macleaya cordata-scutellaria baicalensis composition. The macleaya cordata-scutellaria baicalensis composition comprises a macleaya cordata over-ground non-fruit part (comprising a macleaya cordata over-ground part before blooming and a macleaya cordata over-ground part after blooming) and / or a macleaya cordata over-ground non-fruit part extract, and scutellaria baicalensis and / or a scutellaria baicalensis extract. The composition is applied to preparing veterinary drugs which have anti-inflammatory, bacteriostatic, anti-stress and diarrhea prevention functions, or preparing drug feed additives which are used for prompting the animal growth or improving the feed conversion or improving the animal growth performance; the veterinary drugs or the drug feed additives are good in effect of treating the digestive tract diseases of animals and dairy cow mastitis, and have relatively good functions of resisting stress, resisting inflammation, protecting intestinal health and improving the animal growth performance; and the composition is wide in raw material source and low in cost, is capable of realizing the comprehensive utilization of resources, and is worthy of popularization and application.

The invention discloses a macleaya cordata total alkaloid preparation method. According to the preparation method provided by the invention, technologies of supersonic-wave continuous countercurrent extraction, ceramic membrane purification, sodium filter membrane condensation, intelligent static microwave vacuum drying technology and the like are adopted to prepare the macleaya cordata extract so as to raise the extraction efficiency of macleaya cordata with one-time extraction rate being over 0.7-1%; in addition, the total alkaloid has high purity and good quality; the preparation technology is simple, the technology period is shortened, the energy consumption and production cost are reduced, there is no pollution during the technology process, and the preparation method is applicable to large-scale industrial cleaning production. Waste residues generated after the extraction of the macleaya cordata total alkaloid can be used as organic fertilizers or fuels for reutilization, thus leading to the full application and development of the macleaya cordata resource.

The invention provides a pink plumepoppy herb injection and a preparation method thereof; the pink plumepoppy herb injection is prepared by the raw materials with the following parts by weight: 30-40 parts of pink plumepoppy herb extract, 0-30 parts of latent solvent, 0.5-5 parts of anodyne and 25-70 parts of dispersion medium; the pink plumepoppy herb extract is prepared by the following procedure: pink plumepoppy herb is percolated with sulphuric acid, the percolate is subject to alcohol precipitation and is filtered and then ethanol is recycled; the pink plumepoppy herb injection of the invention can effectively restrain the growth of Gram-positive bacteria and has stronger bacteriostatic action than berberine, and diplococcus pneumoniae, staphylococcus aureus and bacillus subtilis are sensitive to the pink plumepoppy herb injection; meanwhile, the pink plumepoppy herb injection also has the function of resisting nematodes and leptospira and can inhibit and slow the body temperature raise caused by diplococcus pneumoniae; furthermore, the pink plumepoppy herb injection has the efficacies of clearing away heat and toxic materials, resisting bacteria and diminishing inflammation; the indications are yellow and white scour of piglets; the preparation method has convenient operation, high yield, low cost and strong stability of finished products.

A pesticide for preventing and controlling tea diseases and pests is characterized by being prepared by, by weight, 0.25 kg of dried sophora flavescens roots, 0.5 kg of dried chili, 1 kg of Chinese prickly ash, 0.05 kg of derris, 0.01 kg of neutral detergent powder, 0.5 kg of tobacco leaves (stems), 0.25 kg of calcined lime, 0.01 kg of saponin, 1 kg of walnut skins, 1.5 kg of macleaya cordata, 0.01 kg of purple sweet potato anthocyanin, 0.01 kg of purslane extract, 0.03 kg of rosemary essential oil, 0.01 kg of chitin and 0.01 kg of total saponins of gynostemma. The raw materials are easy to obtain, the pesticide is convenient to prepare, economic, capable of saving time, fast in effect, long in lasting period and free of residues, has very good effect of preventing and controlling the tea diseases and pests, has no adverse effect on non-target crops, also reduces environmental influence and meets the green and environmental protection trend of the future society.

The present invention discloses a total alkaloid composite from plant has anticancer activity and its extraction process and use as well as medicine preparation containing the composite and its preparation process. The plant is macleaya cordata, greater celandine herb, adnate elder herb or Asiatic toddalia root. Experiment shows that the preparation has powerful effect of suppressing MRP gene expression and excellent effect of suppressing Ara-C to induce the expression of MDRI, and excellent anticancer effect.

The invention belongs to the field of laying hen feed and provides laying hen brood-time expansion mixed feed and a preparation method thereof. The mixed feed comprises, by weight, 200-380 parts of corn, 150-200 parts of expansion corn, 40-100 parts of wheat, 200-300 parts of expansion soybean meal, 30-80 parts of extruded soybean, 10-20 parts of steam fish meal, 10-15 parts of stone powder, 12-18 parts of calcium hydrophosphate, 1.5-3.5 parts of sodium chloride, 1-2 parts of acidifier, 0.8-1.0 part of compound trace element, 0.3-0.6 part of multi-vitamin, 0.2-0.5 part of probiotics, 0.5-1.0 part of choline chloride, 0.5-1.0 part of methionine, 0.2-0.4 part of aureomycin, 0.1-0.5 part of macleaya cordata extract, 0.1-0.15 part of phytase and 0.1-0.2 part of enzyme preparation.

The invention belongs to the field of plant protection, and relates to an agricultural composition containing antibiotics and plant source materials, wherein antibiotic is one or several selected from abamectin, ivermectin, milbemectin, pleocidin, nikkomycin, liuyangmycin, streptomycin, polyoxins, chunleimeisu, jinggangmeisu, ningnanmycin and the like; the plant source material is one or several of smashing material, crude extract, or active ingredient of derris, thunder god vine, tobacco, pyrethrum, tagetes, crofton weed, radix stemonae, cayenne, macleaya cordata, radix sophorae flavescentis and the like. The composition provided by the invention expresses well synergism in a certain matching range, and has excellent control efficiency for crop diseases and pests.

The invention discloses an anti-coronavirus macleaya cordata benzylisoquinoline alkaloid and resveratrol composition and application thereof. The macleaya cordata benzylisoquinoline alkaloid and resveratrol composition is prepared from five compositions including sanguinarine, chelerythrine, protopine, alpha-allocryptoxine and trans-resveratrol. The macleaya cordata benzylisoquinoline alkaloid andresveratrol composition has unique binding activity on a coronavirus related protein target, has a remarkable anti-coronavirus effect, and is expected to become a raw material of a drug for treatingpneumonia caused by human infection of Covid-19 coronavirus especially when concerning to the inhibition effect on Covid-19 coronavirus. The composition can be used for preparing medicines or other products for resisting diseases caused by coronavirus infection.

The invention discloses a Macleaya cordata total alkaloids water dispersion granule disinfectant, which comprises the following components by weight percentage: 1.5 percent to 5 percent of Macleaya cordata total alkaloids, 10 percent to 30 percent of sasanguasaponin, 1 percent to 15 percent of disintegrant, 1 percent to 15 percent of dispersant, 1 percent to 15 percent of wetting agent, 0.5 percent to 1 percent of caking agent, 0.5 percent to 1 percent of stabilizing agent and the rest of carrier. The invention also discloses a preparation method of the disinfectant. The Macleaya cordata total lakalioids is used for being made into plant farm chemical water dispersion granule, so the application range of the chemical can be enlarged, the lasting period can be prolonged, and the drug action can be improved; not only can the damage to the ecological environment be alleviated, but also the production cost can be reduced, and the toxic effect on different natural enemies can be greatly alleviated; and the disinfectant is convenient to pack, store and transport.

The invention discloses cytochrome P450 enzyme gene for taking part in sanguinarine and chelerythrine synthesis in macleaya cordata and application of the cytochrome P450 enzyme gene. For the first time, five cytochrome P450 enzyme genes taking part in sanguinarine and chelerythrine synthesis are found in macleaya cordata genome and comprise gene Mc9485, gene Mc2661, gene Mc217, gene MC11229 and gene Mc11218. A brewer's yeast system verifies that the steps of reaction are respectively verified by upstream precursor feeding and synthesis of sanguinarine and chelerythrine intermediate is achieved. According to the cytochrome P450 enzyme gene, the conversion efficiencies of the same function genes in the macleaya cordata, the papaver somniferum and the eschscholzia californica are compared by the brewer's yeast heterogeneous expression system, and the conversion efficiency of the macleaya cordata is obviously higher than those of the papaver somniferum and the eschscholzia californica. According to the cytochrome P450 enzyme gene, a molecular mechanism of the sanguinarine synthesis in the macleaya cordata is further revealed. Thus, a theoretical basis and a molecular assistant breeding target are provided for the breeding of the macleaya cordata with high sanguinarine and chelerythrine content. Meanwhile, valuable experience is provided for in-vitro artificial synthesis of the sanguinarine and the chelerythrine.

The invention relates to the technical field of traditional Chinese medicines and in particular relates to a novel method for quickly extracting and separating five types of alkaloids from macleaya cordata. The method comprises the following steps of 1) performing solvent extraction on dry macleaya cordata powder, thus obtaining an extracted solution; 2) extracting the extracted solution sequentially by utilizing ethyl acetate and normal butanol, thus obtaining an ethyl acetate extract and a normal butanol extract respectively; 3) treating the ethyl acetate extract, thus obtaining high-purity sanguinarine and chelerythrine; and 4) treating the normal butanol extract, thus obtaining high-purity protopine, cryptopine and allocryptopine. The method provided by the invention has the advantages of simplicity in operation, high efficiency, low cost, high product purity and product recovery rate and suitability for industrial large-scale production.

The invention relates to the technical field of medicine. Chelerythrine is isoquinoline-type alkaloid existing in plants such as celandine and macleaya cordata, and the structural formula of chelerythrine is shown in (I) described in the specification. The invention provides applications of chelerythrine in preparation of antifungal biofilm medicines, medical equipment or medical materials and the like. Experiments prove that chelerythrine has better antifungal biofilm activity both in vitro and in vivo, can effectively inhibit the growth and proliferation of fungal biofilms, and can effectively inhibit mature biofilms.

The invention relates to a method for extracting waste filtered residual liquid and residue generated by benzophen anthridine total alkaloids and reextracting and preparing an extractive of puteaux total alkaloids by using natural plants (macleaya cordata, celandine, eomecon chionantha and macleaya microcarpa). The method mainly comprises the following steps: extracting benzophen anthridine alkaloid from plants containing benzylisoquinoline alkaloid to obtain filtered residual liquid and residue; and purifying the filtered residual liquid by adsorptive separation of macroporous resin or extracting and separating the residue by using a solvent to obtain the extractive of the puteaux total alkaloid salts with mass percentage of between 50 and 90 percent. The invention provides a novel process which is economical, feasible and suitable for large-scale industrial production for preparing the puteaux total alkaloids with high mass percentage, and is particularly suitable for establishing an 'environmentally-friendly and resource-conserving' natural product development system.