1617 results about "Non target" patented technology

The present invention provides systems, apparatus and methods for selectively applying electrical energy to body tissue in order to ablate, contract, coagulate, or otherwise modify a target tissue or organ of a patients. An electrosurgical apparatus of the invention includes a shaft having a shaft distal end bearing an active electrode and a return electrode. At least one of the active electrode and the return electrode is moveable such that the shaft distal end can adopt a closed configuration or an open configuration. The apparatus can operate in an ablation mode or a sub-ablation mode. The closed configuration is adapted for clamping and coagulating a target tissue while the apparatus is operating in the sub-ablation mode, while the open configuration is adapted for ablating the target tissue via molecular dissociation of tissue components. A method of the present invention comprises clamping a target tissue or organ with an electrosurgical probe. A first high frequency voltage is applied between the active electrode and the return electrode to effect coagulation of the clamped tissue. Thereafter, a second high frequency voltage is applied to effect localized molecular dissociation of the coagulated tissue. The present invention allows the ablation or modification of the target tissue with minimal or no damage to surrounding, non-target tissue.

This invention relates to methods, kits and compositions suitable for the improved detection, analysis and quantitation of nucleic acid target sequences using probe based hybridization assays. The invention is more specifically directed to methods, kits and compositions suitable for suppressing the binding of detectable nucleic acid probes or detectable PNA probes to non-target nucleic acid sequences in an assay for a target nucleic acid sequence to thereby improve the reliability, sensitivity and specificity of the assay. The methods, kits and compositions of this invention are particularly well suited to the detection and analysis of nucleic acid point mutations.

The present invention provides systems, apparatus and methods for selectively applying electrical energy to body tissue in order to incise, dissect, harvest or transect tissues or an organ of a patient. The electrosurgical systems and methods are useful, inter alia, for accessing, dissecting, and transecting a graft blood vessel, such as the internal mammary arteries (IMA) or the saphenous vein, for use in a by-pass procedure. A method of the present invention comprises positioning an electrosurgical probe adjacent the target tissue so that one or more active electrode(s) are brought into at least partial contact or close proximity with a target site in the presence of an electrically conductive fluid. A high frequency voltage is then applied between the active electrode and one or more return electrode(s). During application of the high frequency voltage, the electrosurgical probe may be translated, reciprocated, or otherwise manipulated such that the active electrode is moved with respect to the tissue. The present invention volumetrically removes the tissue at the point of incision, dissection, or transection in a cool ablation process that minimizes thermal damage to surrounding, non-target tissue.

Ultrasound contrast agents are used to enhance imaging and facilitate HIFU therapy in four different ways. A contrast agent is used: (1) before therapy to locate specific vascular structures for treatment; (2) to determine the focal point of a HIFU therapy transducer while the HIFU therapy transducer is operated at a relatively low power level, so that non-target tissue is not damaged as the HIFU is transducer is properly focused at the target location; (3) to provide a positive feedback mechanism by causing cavitation that generates heat, reducing the level of HIFU energy administered for therapy compared to that required when a contrast agent is not used; and, (4) to shield non-target tissue from damage, by blocking the HIFU energy. Various combinations of these techniques can also be employed in a single therapeutic implementation.

The invention provides a method of sensitising target cells to ultrasound energy using a stimulus such as an electric field. This "electrosensitisation" enables target cells to be disrupted by ultrasound at frequencies and energies of ultrasound which do not cause disruption of non-sensitised (i.e., non-target) cells. As a consequence, the method increases the selectivity of ultrasound therapy, providing a way to ablate undesired cells, such as diseased cells (e.g., tumor cells) while minimising harm to neighboring cells. In another aspect, however, ultrasound can be used to sensitise cells while the electrical field is used to disrupt cells. The invention also provides an apparatus for performing the method and assays for identifying gene products and other molecules involved in apoptosis.

The present invention provides method and apparatus for treating tissue in a region at depth while protecting non-targeted tissue by cyclically applying cooling to the patients skin, and preferably to the region, and by applying radiation to the patient's skin above the region to selectively heat tissue during and / or after cooling is applied. At least one of cooling and radiation my be applied by successivly passing a continuous output applicator over the patient's skin. Treatment may also be enhanced by applying mechanical, acoustic or electrical stimulation to the region.

A probe and method for removing tissue is provided. The probe comprises an elongated member, a window laterally formed on the distal end of the member, a drive shaft rotatably disposed within the lumen of the member, and a rotatable and longitudinally slidable tissue removal element disposed on the drive shaft. The probe can be used to remove tissue along the window of the probe. In one method, target tissue along the window, e.g., bone tissue, can be removed without removing non-target tissue, e.g., nerve tissue, by rotating and longitudinally sliding the tissue removal element relative to the window.

There are provided a vascular information acquisition device, an endoscope system, and a vascular information acquisition method that can accurately acquire vascular information on a blood vessel of a target layer that is an object to be measured of a subject. A first blood vessel extraction unit (82) analyzes the image of a target layer to be measured and extracts a blood vessel (first blood vessel) from the image of a target layer. A blood vessel specification unit (84) specifies a blood vessel (second blood vessel) extending to a non-target layer from the target layer. In a case in which the second blood vessel is specified, a second blood vessel extraction unit (83) analyzes the image of the non-target layer in which the second blood vessel is present and extracts the specified second blood vessel from the image of the non-target layer.

Ultrasound contrast agents are used to enhance imaging and facilitate HIFU therapy in four different ways. A contrast agent is used: (1) before therapy to locate specific vascular structures for treatment; (2) to determine the focal point of a HIFU therapy transducer while the HIFU therapy transducer is operated at a relatively low power level, so that non-target tissue is not damaged as the HIFU is transducer is properly focused at the target location; (3) to provide a positive feedback mechanism by causing cavitation that generates heat, reducing the level of HIFU energy administered for therapy compared to that required when a contrast agent is not used; and, (4) to shield non-target tissue from damage, by blocking the HIFU energy. Various combinations of these techniques can also be employed in a single therapeutic implementation.

The invention provides a method of sensitizing target cells to ultrasound energy using a stimulus such as an electric field. This "electrosensitisation" enables target cells to be disrupted by ultrasound at frequencies and energies of ultrasound which do not cause disruption of non-sensitized (i.e., non-target) cells. As a consequence, the method increases the selectivity of ultrasound therapy, providing a way to ablate undesired cells, such as diseased cells (e.g., tumor cells) while minimizing harm to neighboring cells. In another aspect, however, ultrasound can be used to sensitize cells while the electrical field is used to disrupt cells. The invention also provides an apparatus for performing the method and assays for identifying gene products and other molecules involved in apoptosis.

The invention provides hollow-tip-electrodes for spatially localized delivery of substances to one or more biological targets present in a population comprising target and non-target molecules, macromolecules, and / or cells. The invention also provides electrode plates for receiving one or more of such tips, tip-electrode plates comprising electrode plates comprising one or more electrode tips, and systems comprising tip-electrodes and containers for containing one or more biological targets, e.g., such as molecules, macromolecules, and / or cells. The invention further provides methods for using such systems and components thereof. In one preferred aspect, the systems are used for spatially confined electroporation of cells and cell structures. The invention facilitates high throughput screening of agents (e.g., such as drugs) that act on intracellular targets.

A medical system comprises a plurality of electrodes; at least one sensor configured to output at least one signal based on at least one physiological parameter of a patient; and a processor. The processor is configured to control delivery of stimulation to the patient using a plurality of electrode configurations. Each of the electrode configurations comprises at least one of the plurality of electrodes. For each of the electrode configurations, the processor is configured to determine a first response of target tissue to the stimulation based on the signals, and a second response of non-target tissue to the stimulation based on the signals. The processor is also configured to select at least one of the electrode configurations for delivery of stimulation to the patient based on the first and second responses for the electrode configurations. As examples, the target tissue may be a left ventricle or vagus nerve.

The present invention provides systems, apparatus and methods for selectively applying electrical energy to body tissue in order to, ablate, contract, coagulate, or otherwise modify a tissue or organ of a patient. An electrosurgical apparatus includes an electrode support bearing an active electrode in the form of a plasma blade or hook having an active edge and first and second blade sides. The active edge is adapted for severing a target tissue via localized molecular dissociation of tissue components. The first and second blade sides are adapted for engaging against, and coagulating, the severed tissue. s. A method of the present invention comprises positioning an electrosurgical probe adjacent to the target tissue so that a blade- or hook-like active electrode is brought into at least close proximity to the target tissue in the presence of an electrically conductive fluid. A high frequency voltage is applied between the active electrode and a return electrode to effect cool ablation or other modification of the target tissue. During application of the high frequency voltage, the electrosurgical apparatus may be translated, reciprocated, or otherwise manipulated such that the active edge is moved with respect to the tissue. The present invention volumetrically ablates or otherwise modifies the target tissue with minimal or no damage to surrounding, non-target tissue.

The invention discloses a parallax optimization algorithm-based binocular stereo vision automatic measurement method. The method comprises the steps of 1, obtaining a corrected binocular view; 2, matching by using a stereo matching algorithm and taking a left view as a base map to obtain a preliminary disparity map; 3, for the corrected left view, enabling a target object area to be a colorized master map and other non-target areas to be wholly black; 4, acquiring a complete disparity map of the target object area according to the target object area; 5, for the complete disparity map, obtaining a three-dimensional point cloud according to a projection model; 6, performing coordinate reprojection on the three-dimensional point cloud to compound a coordinate related pixel map; 7, using a morphology method to automatically measure the length and width of a target object. By adopting the method, a binocular measuring operation process is simplified, the influence of specular reflection, foreshortening, perspective distortion, low textures and repeated textures on a smooth surface is reduced, automatic and intelligent measuring is realized, the application range of binocular measuring is widened, and technical support is provided for subsequent robot binocular vision.

A laser-based method and system for processing targeted surface material and article produced thereby are provided. The system processes the targeted surface material within a region of a workpiece while avoiding undesirable changes to adjacent non-targeted material. The system includes a primary laser subsystem including a primary laser source for generating a pulsed laser output including at least one pulse having a wavelength and a pulse width less than 1 ns. A delivery subsystem irradiates the targeted surface material of the workpiece with the pulsed laser output including the at least one pulse to texture the targeted surface material. The pulsed laser output has sufficient total fluence to initiate ablation within at least a portion of the targeted surface material and the pulse width is short enough such that the region and the non-targeted material surrounding the material are substantially free of slag.

A method and apparatus are disclosed for liquefying target tissue within a body and aspirating the same while leaving non-target tissue intact. A biocompatible fluid is heated and contacted with target tissue so that the target tissue is melted while non-target tissue remains intact. As the target tissue is being melted it is also aspirated from the body.

Acoustic shielding system and method for protecting and shielding non-targeted regions or tissues that are not intended to be treated by ultrasonic procedures from acoustic energy using a shield. In some embodiments, the shield comprises multiple layers made of one or more materials with one or more acoustic impedances. In some embodiments a multilayered shield includes materials with relatively different acoustic impedance levels. In some embodiments, the shield includes active components such as energy diversion devices, heating, cooling, monitoring, and / or sensing. In some embodiments, the shield is configured to protect an eye, mouth, nose or ear while allowing the ultrasound to treat the surrounding tissue. One embodiment of an eye shield is configured to fit under at least one eyelid and over a portion of the eye.

A test apparatus and method for design verification of at least one microprocessor chip includes a compatible Joint Task Action Group (JTAG) terminal for access to a plurality of computer functional units contained in the chip. A test input terminal included in the JTAG terminal receives a scan string, the string being coupled to each computer functional unit through a first multiplexer. The scan input string is separated by the JTAG terminal under program control into a series of dedicated scan strings, each dedicated scan string being supplied to a selected functional unit through the first multiplexer. Each functional unit includes start and stop scan clocks for testing the functional under program control using the dedicated scan train for the functional unit. A test output terminal included in the JTAG terminal is coupled to each functional unit through a second multiplexer. The test results of the dedicated scan string under control of the scan clock are supplied to the output terminal through the second multiplexer. The compatible JTAG terminal includes further elements for controlling the scan clocks to select a targeted functional unit for testing purposes while the scan strings for non-targeted functional units remain in an inactive state.

A method of assay for target polynucleotides includes steps of isolating target polynucleotides from extraneous non-target polynucleotides, debris, and impurities and amplifying the target polynucleotide.

A communication network supports push-to-talk communications with reduced dispatching delays by eliminating channel grant / request handshaking on the uplink and the downlink. A pool of communication channels can be associated with a group of nets that include one or more mobile terminals. Any terminal can send dispatch traffic immediately on an assigned uplink channel simply by seizing the channel. The network detects such traffic and retransmits it on a downlink channel monitored by the group of nets. Along with the retransmitted traffic, the network sends a net ID and a new downlink channel assignment. Terminals in the targeted net process the retransmitted traffic and non-targeted terminals switch to the new channel. Alternatively, the control channel can be multiplexed to signal traffic channel assignments as needed for all nets, and traffic can be transmitted on the indicated channels for reception by targeted terminals. The reduced delays particularly benefit networks incorporating satellite relays.

Described herein are devices, systems and methods for treating target tissue in a patient's spine. In general, the methods include the steps of advancing a wire into the patient from a first location, through a neural foramen, and out of the patient from a second location; connecting a tissue modification device to the wire; positioning the tissue modification device through the neural foramen using the wire; modifying target tissue in the spine by moving the tissue modification device against the target tissue; and delivering an agent to modified target tissue, wherein the agent is configured to inhibit blood flow from the modified target tissue. In some embodiments, the step of modifying target tissue comprises removing target tissue located ventral to the superior articular process while avoiding non-target tissue located lateral to the superior articular process.

A medical assembly and method are provided to effectively treat abnormal tissue, such as, a tumor. The target tissue is thermally ablated using a suitable source, such as RF or laser energy. A cooling shield is placed in contact with non-target tissue adjacent the target tissue, and actively cooled to conduct thermal energy away from the non-target tissue. In one method, the cooling shield can be placed between two organs, in which case, one of the two organs can comprise the target tissue, and the other of the two organs can comprise the non-target tissue. In this case, the cooling shield may comprise an actively cooled inflatable balloon, which can be disposed between the two organs when deflated, and then inflated. The inflatable balloon can be actively cooled by pumping a cooling medium through it. In another method, the cooling shield can be embedded within the non-target tissue. In this case, the cooling shield can comprise one or more needles. If a plurality of needles is used, they can be embedded into the non-target tissue in a series, e.g., a rectilinear or curvilinear arrangement. The needle(s) can be actively cooled by pumping a cooling medium through them.

Compositions and methods for stabilizing rare cells in blood specimens, preserving the quality of blood specimens, and also serving as cell fixatives are disclosed which minimize losses of target cells (for example, circulating tumor cells) and formation of debris and aggregates from target cells, non-target cells and plasma components, thereby allowing more accurate analysis and classification of circulating tumor cells (CTC) and, ultimately, of tumor burdens in cancer patients. Stabilization of specimens is particularly desirable in protocols requiring rare cell enrichment from blood specimens drawn from cancer patients. Exposure of such specimens to potentially stressful conditions encountered, for example, in normal processing, mixing, shaking, delays due to transporting the blood, has been observed to not only diminish the number of CTC but also to generate debris and aggregates in the blood specimens that were found to interfere with accurate enumeration of target cells, if present. Stabilizers are necessary to discriminate between in vivo CTC disintegration and in vitro sample degredation.

A pop-up toolbar is disclosed which is demonstrably faster and easier to utilize than the permanently displayed, horizontal toolbar. Two columns provide a variable number of irregularly shaped regions arrayed on either side of a generally circular central-area. Components of regions converge on the central area. Targets, each displaying an icon and an optional, descriptive label, appear within the regions. At pop-up of the toolbar, the cursor moves under system control to the central-area of the toolbar, enabling users to perform short, radial traverses of the cursor into targets, activating related functions by appropriate action. Alternatively, primary and secondary targets for regions may be designated for remote acquisition, whereby alternate user actions in any non-target area of a region activate the primary and secondary targets respectively defined for that region. The central-area is divided into a varying number of shapes permitting users to activate application, system, or toolbar management activities.

The present invention provides methods for enhancing discriminatory RNA silencing by RNA silencing agents. In particular, the invention provides methods for generating RNA silencing agents which can discriminate between target and non-target mRNAs that differ in sequence by only one nucleotide. Also provided are improved RNA silencing agents with enhanced discriminatory RNA silencing, e.g., single nucleotide discriminatory RNA silencing. The compositions and methods of the invention are useful in therapeutic strategies for treating genetic disorders associated with dominant, gain-of-function gene mutations.

The invention provides a contour vector feature-based embedded real-time image matching method. The method uses the linear feature based on X and Y direction vectors, and has strong capability of resisting image distortion, noise, shading, illumination changes, polarity inversion and so on. An image pyramid search strategy is used, templates are quickly matched in a high-layer low-resolution image to be tested, and then, a target position is found out accurately by stepwise downward search, so that matching time is reduced greatly. According to the template image specific information, the best pyramid hierarchy number and the best rotation angle step size for the pyramid template matching of each layer are calculated automatically. An image pyramid highest-layer three-level screening matching strategy is provided, treatment is carried out according to the specific content of the image to be tested, and the first level of screening and the second level of screening are carried out; the non-target position is eliminated just by the addition and subtraction and the conditional statements, which is more efficient in the embedded system than using the multiplication and division; and the third level only processes fewer positions meeting the requirements of the above two levels, so that the matching speed is improved greatly. The overall method can realize the work of matching and locating the target at any angle and any coordinate.

A probe and method for removing tissue is provided. The probe comprises an elongated member, a window laterally formed on the distal end of the member, a drive shaft rotatably disposed within the lumen of the member, and a rotatable tissue removal element disposed on the drive shaft. The tissue removal probe further comprises irrigation and aspiration lumens extending through the member in fluid communication with the window. In one method, target tissue, e.g., bone tissue, can be removed without removing non-target tissue, e.g., nerve tissue, by rotating the tissue removal element relative to the window. Fluid can be conveyed through the irrigation lumen into contact with the rotating tissue removal element. As a result of the rotation of the tissue removal element, the tissue is irrigated and removed, and forced towards the aspiration lumen. The irrigation fluid and removed tissue is then aspirated into the aspiration lumen.

A computer-implemented system and method analyzes the effectiveness of pharmaceutical treatments for medical conditions utilizing real time prescription compliance records. Information related to medical history of a patient can be reviewed while simultaneously reviewing prescription compliance records for the same patient. Trends in a target or non-target medical parameter can be identified and correlated with the prescription compliance records to determine the effectiveness of a pharmaceutical treatment on the target and non-target medical parameter. Correlations can be identified for a group of patients having the same medical condition.

The invention discloses a liquid nitrogen cylinder, a cryogenic vial storing and fetching device and a liquid nitrogen cylinder storing and fetching system. The liquid nitrogen cylinder comprises a cylinder body, wherein the cylinder body is provided with a sealed opening; the liquid nitrogen cylinder further comprises a bracket tray; the bracket tray is arranged in the cylinder body; the bracket tray is used for bearing cryogenic baskets; a central shaft is vertical to the bracket tray and is fixedly connected with the bracket tray; a driving component is electrically connected with the central shaft; the driving component is used for driving the central shaft to rotate; and the central shaft is used for driving the cryogenic baskets to rotate through the bracket tray and conveying the cryogenic baskets to the opening. By adopting the liquid nitrogen cylinder, the error rate of manual cryogenic vial selecting is reduced; and meanwhile, safety hazards for operation personnel during a manual bracket tray rotation process are avoided. By adopting the cryogenic vial storing and fetching device, the process for selecting a single target cryogenic vial for biological samples is realized; and during the whole selecting process, the cryogenic vials are all in a liquid nitrogen environment, so that non-target cryogenic vials are prevented from being exposed at environment temperature. The liquid nitrogen cylinder storing and fetching system is applicable to vial picking work for large biological sample banks.

Acoustic shielding system and method for protecting and shielding non-targeted regions or tissues that are not intended to be treated by ultrasonic procedures from acoustic energy using a shield. In some embodiments, the shield comprises multiple layers made of one or more materials with one or more acoustic impedances. In some embodiments a multilayered shield includes materials with relatively different acoustic impedance levels. In some embodiments, the shield includes active components such as energy diversion devices, heating, cooling, monitoring, and / or sensing. In some embodiments, the shield is configured to protect an eye, mouth, nose or ear while allowing the ultrasound to treat the surrounding tissue. One embodiment of an eye shield is configured to fit under at least one eyelid and over a portion of the eye.