The invention relates to a tincture for treating traumatic injuries, water and fire burns, and snake and insect bites, and a preparation method thereof. The tincture comprises a tincture component 1 and a tincture component 2, wherein the tincture component 1 comprises 2-50 parts of raw radix aconiti, 2-60 parts of raw radix aconiti kusnezoffii, 2-60 parts of raw strychnos nux-vomica l, 2-60 partsof herba asari heterotropoidedis, 1-40 parts of ligusticum chuanxiong hort, 1-40 parts of zanthoxylum schinifolium sieb.et zucc, 1-40 parts of common clubmoss herb, 1-60 parts of rhizoma corydalis, 1-30 parts of saposhnikovia divaricata, 1-30 parts of notopterygium incisum, 1-10 parts of radix angelicae pubescentis, 1-50 parts of datura metel l, 1-40 parts of cinnamomum cassia, 2-40 parts of frankincense, 1-20 parts of myrrh, 1-50 parts of resina draconis, 1-30 parts of shortstalk monkshood root, 1-25 parts of wikstroemia indica, 1-70 parts of paris polyphylla, 1-40 parts of piper nigrum l, 1-20 parts of pericarpium papaveris, 1-30 parts of arisaema cum bile, 3-70 parts of semen sinapis, and 1-10 parts of piper nigrum l, and the tincture component comprises 1-20 parts of peppermint, 2-20parts of borneol, 1-50 parts of camphor, 1-20 parts of musk, and 2-20 parts of venenum bufonis. The preparation method comprises: adding 3000 ML 75% alcohol to the tincture component 1, soaking for 30days, adding the tincture component 2, soaking for 7 days, and sealing for spare. According to the present invention, the tincture has advantages of reasonable composition, less medication amount, environmental protection, simple operation, significant treatment effect and low toxic-side effect, can stop pain and itching, can treat traumatic injuries, water and fire burns, and snake and insect bites, and can fill gaps at home and abroad.