The invention discloses a method for detecting an
odor substance butanedione based on an
olfactory receptor sensor. The method comprises the following steps of: at first, expressing
olfactory receptor protein ODR-10 with a His6-tag on an HEK-293
cell surface, extracting and effectively immobilizing the
olfactory receptor protein ODR-10 on a
quartz crystal microbalance surface through an
aptamer so as to form the olfactory
receptor sensor; testing a series of
odor material butanedione with different set concentrations, thus obtaining a butanedione-
resonance frequency variation
standard curve; and then, testing a gas which has unknown concentration and needs to be tested, thereby obtaining the concentration of the
odor substance-butanedione in the gas to be tested according to the
quartz crystal microbalance resonant frequency and the butanedione-
resonance frequency variation
standard curve. The method is simple in required instrument and convenient to operate, a problem that the olfactory
receptor protein ODR-10a of a sensitive element and a
quartz crystal microbalance are stably coupled with each other is solved, and the odor substance butanedione is quickly and specially detected.