266 results about "Cyclic voltametry" patented technology

The invention discloses a method for detecting aflatoxin through an immunosensor. The aflatoxin (AFT) is a fungal toxin which has the high carcinogenicity, teratogenicity and toxicity, and accurate and rapid analysis on the aflatoxin is one of most effective means for reducing and avoiding harm of the aflatoxin. A prepared gold-polyaniline-graphene (Au-PANI-GN) composite nanomaterial is represented by adopting an ultraviolet spectrum and a transmission electron microscope and used for constructing the electrochemical immunosensor, the electrochemical immunosensor is represented by adopting a cyclic voltammetry method, an electrochemical AC impedance method and a square wave voltammetry method, and the prepared electrochemical immunosensor detects the aflatorxin B1. Experiment results show that the constructed electrochemical immunosensor has the good electrochemical activity on detection of the aflatoxin, and the concentrations of the aflatoxin and peak currents have the good linear relation, wherein the linear equation of the relation is y=-0.177x+4.28.

The invention discloses a ferro-nickel oxyhydroxide-modified bismuth vanadate photoelectrode and a preparation method and application thereof. The preparation method comprises the following steps: firstly, depositing bismuth oxyiodide on the surface of conductive glass, then coating the surface with the deposited bismuth oxyiodide with a dimethyl sulfoxide solution of vanadyl acetylacetonate, annealing, performing alkali soaking and rinsing with water to remove excessive vanadium pentoxide, and then drying to obtain a bismuth vanadate photoelectrode, and modifying ferro-nickel oxyhydroxide on the surface of the bismuth vanadate photoelectrode by adopting a cyclic voltammetry method in a three-electrode system, thus obtaining the ferro-nickel oxyhydroxide-modified bismuth vanadate photoelectrode. The invention further discloses applications of the ferro-nickel oxyhydroxide-modified bismuth vanadate photoelectrode in photoelectrocatalytic decomposition water. The prepared photoelectrode is used for producing hydrogen from photoelectrocatalytic decomposition water, can inhibit the compounding of photon-generated carriers, the service life of carriers generated by a BiVO4 photoelectrode can be effectively prolonged, and the oxygen evolution reaction on the surface of the photoelectrode can be promoted, so that the solar optic hydrogen conversion efficiency of a semiconductor photoelectrode can be improved.

The invention discloses a preparation method for a graphene / conductive polymer anode for a microbial fuel cell. The preparation method comprises the following steps: conductive polymer monomers and an aqueous suspension of graphene oxides are mixed, stirred at room temperature and subjected to ultrasonic treatment; by employing a constant voltage electroplating method, conductive polymer monomer / graphene oxide conductive composites are subjected to electrochemical polymerization and deposited on a surface of an anode; by employing a cyclic voltammetry, after situ electroreduction, a conductive polymer / electrochemical reduction graphene oxide modified anode is prepared on the electrode. The conductive polymer / electrochemical reduction graphene oxide modified anode is washed with deionized water and dried in the air at room temperature to prepare a graphene / conductive polymer anode for a microbial fuel cell. Compared to traditional chemical modification methods, the preparation method reduces the usage of toxic reagents and cumbersome processes, lowers the preparation cost, and is easy to realize industrialization of electrode preparation. When the modified electrode is used for a cell, the electricity generation capacity of a microbial fuel cell is raised observably, and the development and application of a microbial fuel cell can be promoted.

The invention provides a method of depositing a metal organic framework material by an oxygen auxiliary cathode. The method comprises the following steps: (1) preparing a reaction precursor solution which is composed of a 5-50mM metal source, a 10-250mM organic ligand and an organic solvent; and (2) carrying out electrochemical deposition on the surface of a conducting substrate of the cathode toobtain the material. The method provided by the invention does not corrode the deposited electrode, the selecting range of the conducting substrate is wide, the prepared MOF material is regulatable inshape, dimension and thickness in nanoscale, pure, free of adding a supporting electrolyte and low in cost, and can be synthesized in one step. The metal organic framework is simple in method and operation, and industrial production is conveniently achieved.

The invention discloses a double recognition dopamine imprinting electrochemical sensor and a preparation method and application thereof. The method comprises the following steps: (1) conducting contact reaction on 3-amino boronic acid, 2,5-dimethoxy furan and acetic acid to prepare functional monomers; (2) in the presence of a buffer solution, mixing the functional monomers and dopamine to form a polymerization liquid, placing a glassy carbon electrode in the polymerization liquid, and conducting scanning preparation of a polymer film modified electrode in the polymerization liquid in accordance with the a cyclic voltammetry; and (3) placing the polymer film modified electrode in an acidic solution for electrochemical elution, so as to prepare the double recognition dopamine imprinting electrochemical sensor. The electrochemical sensor provided by the invention has excellent specificity recognition ability and anti-interference ability for the detection of dopamine.

The invention discloses preparation of an electro-polymerization molecularly imprinted polymer hollow channel paper device and an application of the paper device in instant pesticide residue detection. A wax printing pattern and a hollow channel cutting pattern are designed on a computer by utilizing AdobeIllustratorCS4 software; a hydrophobic area can be printed by a wax printing machine; an entrance, an exit and a hollow channel are cut by a laser cutting machine; a carbon working electrode, a carbon counter electrode and an Ag / AgCl reference electrode are printed on a working area by the screen printing technique; molecularly imprinted polymers are electro-polymerized on the working electrode by a cyclic voltammetry method; a plurality of pieces of prepared filter paper are stacked between glass sheets sequentially to prepare the electro-polymerization molecularly imprinted polymer hollow channel paper device, and the electrode in the device is connected with an electrochemical workstation to realize instant detection of pesticide residues.

A method and biosensor device for detecting single strand target nucleic acid by cyclic voltammetry is described. A porous silicon chip is linked to bound DNA probe complementary to the target nucleic acid. The device is particularly useful for detecting microorganisms and viruses that may be pathogenic or cancer genes, however any target nucleic acid can be detected by using a specific DNA probe.

The invention provides an electrocatalytic hydrogen production porous high-entropy alloy electrode material and a preparation method thereof. The preparation method comprises the following steps: S1,firstly, mixing cobalt powder, chromium powder, iron powder, nickel powder and molybdenum powder, carrying out uniform ball milling by using a planetary ball mill, then adding magnesium powder and carrying out uniform ball milling by using the planetary ball mill, and finally, pressing the mixed powder into a sample blank by using a sample pressing machine; S2, placing the sample blank in a polycrystalline mullite fiber heat preservation barrel, and then placing the heat preservation barrel in a microwave sintering furnace to be subjected to microwave sintering; S3, closing the microwave furnace for cooling along with the furnace, and then closing the microwave sintering furnace, so that the sample is cooled to room temperature along with the furnace to obtain a porous high-entropy alloy;S4, treating the porous high-entropy alloy through cyclic voltammetry for electrochemical activation, thereby preparing the porous high-entropy alloy electrode material. The porous high-entropy alloyelectrode material prepared by the method has the advantages of three-dimensional porous self-supporting structure, high strength, large active specific surface area, low overpotential, small tafel slope, corrosion resistance and the like.

The invention relates to a method for the electrochemical detection of sequence-specific nucleic acid oligomer hybridization events. To this end single DNA / RNA / PNA oligomer strands which at one end are covalently joined to a support surface and at the other, free end, covalently linked to a redox pair, are used as hybridization matrix (probe). As a result of treatment with the oligonucleotide solution (target) to be examined, the electric communication between the conductive support surface and the redox pair bridged by the single-strand oligonucleotide, which communication initially is either absent or very weak, is modified. In case of hybridization, the electric communication between the support surface and the redox pair, which is now bridged by a hybridized double-strand oligonucleotide, is increased. This permits the detection of a hybridization event by electrochemical methods such as cyclic voltametry, amperometry or conductivity measurement.

The invention discloses a process for preparing high density polypyrrole film anti-corrosion coat, wherein the solution A is produced by adding pyrrole monomer into oxalic acid radical solution; the solution B is produced by dissolving methyl benzene sulfonic acid into acetonitrile solution, then the protected copper or copper alloy is immerged into the solution A, and polymerize by an electrochemical process using cyclic voltammetry to produce polypyrrole film-modified copper or copper alloy electrode C; lastly, copper or copper alloy electrode C is immerged into the solution B, and polymerize by an electrochemical process to produce a layer of high density polypyrrole film D on the surface of copper or copper alloy electrode C. The film produced on the surface of copper by the invention is more compact than generally-synthetical polypyrrole film, can efficiently prevent corrosion ion from penetrating through the coat for a long time, and improve the corrosion potential of copper and reduce corrosion current, while the coat has higher adhesivity, but also corrosion of metal can be avoided by producing passivating film after scuffing the coat.

The invention discloses a method for preparing a biosensor based on silicon nanowires and application of the biosensor to detecting DNA. The method is characterized by preparing the silicon nanowires by a wet chemical method, modifying gold nanoparticles on the silicon nanowires via a silane coupling agent and grafting a probe DNA on the silicon nanowires through binding of chemical bonds betweenthe gold nanoparticles and the DNA to prepare a sensor probe. The sensor is applied to detecting the unknown DNA sequences in the target solution to be detected. The detecting results are mainly obtained by analyzing the data measured by cyclic voltammetry. The invention has the following advantages: (1) mass production can be carried out through simple microprocessing technology, the cost is lowand the microprocessing technic is compatible with the large scale integration technology; (2) the biosensor mainly utilizes the specificity and biocompatibility among the silicon nanowires, the goldnanoparticles and the DNA, is easy to realize and has wide applicability; and (3) the biosensor is simple and convenient to manufacture, has good repeatability and high sensibility, is easy to realize microminiaturization and can realize real-time monitoring.

The invention belongs to the technical field of material science and engineering and relates to a pressure molding preparing method for preparing metal and an alloy micro-nano structure or nanowire array of the metal through compression molding. The method includes the following steps that firstly, a metal sheet or an alloy sheet is subjected to surface treatment; secondly, a mold plate is prepared; thirdly, compression molding is conducted; fourthly, demounting is conducted; and fifthly, demolding is conducted. By means of the method, the requirement for a complex cyclic voltammetry pulse electrodeposition technology is avoided, no organic surface active agent needs to be added, and complex sensitizing and activating treatment does not need to be conducted on a hole wall of the mold plate. By the adoption of the method, large-range and uniform metal nanowires (such as Au, Ag, Cu, Fe and Ni nanowires) similar to single crystals and gold and silver solid solution alloy nanowires (such as Au26Ag74 nanowires) can be obtained. By means of the preparing method, low-cost controllable batched preparation of metal and alloy micro-nano structures or nanowires of the metal can be achieved, and the preparing method is of important and profound significance in revealing basic performance principles of metal and alloys of the metal or wide and deep application.

The invention discloses a silicon nanometer wire-conductive polymer PEDOT (polyethylenedioxythiophene) compound as well as a preparation method and an application thereof. The preparation method comprises the following steps: 1) using an HF (Hydrogen Fluoride) solution to treat the surface of a silicon nanometer wire array structure which is acquired by using a metal nanometer particle catalyzing auxiliary etching method, thereby acquiring a treated silicon nanometer wire array structure; and 2) using a cyclic voltammetry to enable a polymer monomer EDOT (ethylenedioxythiophene) to have electrochemical polymerization on the surface of the treated silicon nanometer wire array structure, thereby acquiring a silicon nanometer wire-conductive polymer compound. The preparation method has a mild reaction condition, is easily operated and can be used for efficiently controlling the thickness of functional polymers on the silicon nanometer wire surface, the band structure and the conductivity. The preparation method is an effective method for constructing a compound structure of the silicon nanometer wire-conductive polymer functional compound. The application of the acquired compound structure in the field of photocatalytic hydrogen production is researched and a result shows that the compound structure has high catalytic property.

The invention discloses a Schiff base surface active agent named 2-octanone condensation-compound ethylenediamine and application thereof. The structural formula of the 2-octanone condensation-compound ethylenediamine is as follows. The 2-octanone condensation-compound ethylenediamine is applied to alkaline Zn-Ni batteries as mercury substituting corrosion inhibition additives and used for preventing corrosion, dendritic growth, deformation, migration and the like of zinc electrodes. The 2-octanone condensation-compound ethylenediamine can effectively prevent alkaline electrolytes from corroding the zinc electrodes. Moreover, the cyclic voltammetry and zinc migration experiments prove that the 2-octanone condensation-compound ethylenediamine plays good roles in inhibiting the dendritic growth, the deformation and the migration of the pasted zinc electrodes.

The present invention relates to a method of activating membrane electrode assemblies of polymer electrolyte membrane fuel cells of a fuel cell stack for a vehicle comprising: supplying a humidified gas to a fuel cell so as to hydrate an electrolyte membrane and an electrolyte of electrodes of the fuel cell; and performing a cyclic voltammetry process so as to activate the layers of the electrodes.

Electropolymerization of EC monomers is employed to obtain an EC polymer film deposited on a substrate. A first embodiment of a method to produce the film employs cyclic voltammetry alone, while a second embodiment deposits a very thin homogeneous layer using chronoamperometry, and then cyclic voltammetry is employed to increase the density of the film. Another aspect of the present invention is directed to specific web like configurations for a grid of conductive material deposited onto a transparent substrate. The web like configuration is based either on concentric circles, or on concentric ellipses. Yet another aspect of the present invention is directed to an imaging system including a digital window that is disposed between a prism and a patterned analytic layer.

The invention relates to the technical field of catalyst preparation, in particular to a preparation method of a catalyst for electrocatalytic reduction of nitrate. The method comprises the followingsteps: by using foamed nickel as a substrate, forming a nickel oxide layer on the surface of the foamed nickel to obtain a compound; and depositing ruthenium nanoparticles on the compound by adoptinga ruthenium trichloride solution and adopting an electrochemical cyclic voltammetry to obtain the NiRu composite catalyst. According to the preparation method of the catalyst for electrocatalytic reduction of nitrate, the preparation method is simple in process, the catalyst load Ru amount is low, and the catalyst cost is low; the invention also provides an application of the catalyst for electrocatalytic reduction of nitrate. The catalyst has higher current density, higher current Faraday efficiency and higher nitrate elimination rate of synthetic ammonia in a wide voltage range.

The invention relates to a manufacture method and application of an immune sensor based on a polyaniline nano-particle composite membrane. By manufacturing the polyaniline nano-particle composite membrane, self-conductivity of materials is improved, and the membrane can be successfully applied to the immune sensor so as to greatly reduce detection limits of the sensor and achieve better stability. Representation and measurement of the sensor are performed through a cyclic voltammetry method and an alternate current impedance method, a staphylococcus aureus enterotoxin B detection standard curve is built, the linear range ranges from 0.1ng / ml to 8ng / ml, correlation coefficient R2=0.9932, and detection limit is 0.033ng / ml(S / N=3). The manufacture method and the application are good in specificity, can be used repeatedly, are good in stability, can be applied to fast detection of staphylococcus aureus enterotoxin B in dairy products and has wide application prospect, and dairy product detection recovery rate ranges from 84% to 111%.

The invention discloses a perovskite solar cell capable of light incoming from double surfaces. The perovskite solar cell comprises a transparent conductive substrate A, a photo anode layer, a perovskite absorption layer, a hole transmission layer, a counter electrode layer and a transparent conductive substrate B, wherein the counter electrode layer is a transparent polymer counter electrode layer. A fabrication method of the perovskite solar cell comprises the following steps of: (1) fabricating the photo anode layer on the transparent conductive substrate A; (2) fabricating the perovskite absorption layer on the photo anode layer; (3) fabricating the hole transmission layer on the perovsite absorption layer; and (4) fabricating the transparent polymer counter electrode layer on the transparent conductive substrate B through electrochemical polymerization by a cyclic voltammetry method, covering the fabricated transparent polymer counter electrode layer on the hole transmission layer, and drying to obtain the perovskite solar cell capable of light incoming from double surfaces. In the perovskite solar cell capable of light incoming from double surfaces, disclosed by the invention, polyaniline, polypyrrole or polythiophene is taken as a transparent counter electrode, and the transparent counter electrode is lower in cost than a nanocrystalline thin-film counter electrode or platinum counter electrode.

The invention belongs to the electrochemical detection technical field, and discloses a molecularly imprinted electrochemical sensor for detecting bisphenol A and a preparation method and an application thereof. A glassy carbon electrode modified by a gold and reduced graphene oxide nano composite material is used as a working electrode, a poly-o-phenylenediamine and bisphenol A mixed film is prepared by cyclic voltammetry, and finally, the bisphenol A is eluted to obtain the molecularly imprinted electrochemical sensor. The content of bisphenol A in a soil sample is detected by differential pulse voltammetry, and compared with a high performance liquid chromatography method, the methods have good correlation. The imprinted electrochemical sensor has the characteristics of fast response, high sensitivity, strong selectivity, wider linear range, high practicability, easy popularization and the like.

The invention relates to the technical field of electrochemical immunosensors, in particular to a construction method of a nanoprobe C60 based electrochemical immunosensor for carcino-embryonic antigens.The method includes: modifying gold nanoclusters on the surface of a glassy carbon electrode by means of cyclic voltammetry to obtain an antibody capture substrate; sequentially immobilizing first antibodies and antigens to the surface of the electrode; immobilizing AuNPs@L-cys-C60 marked second antibodies by specific binding of the antigens and the antibodies.The gold nanoclusters have excellent biocompatibility and electrical conductivity and large surface areas and are capable of promoting electron transferring between proteins and the electrode.The AuNPs@L-cys-C60 serves as an oxidation-reduction nanoprobe, the immunosensor is used for detection of the carcino-embryonic antigens on the basis of excellent specificity between the antigens and the antibodies, and detection of the carcino-embryonic antigens is realized according to different electrochemical signals of the carcino-embryonic antigens with different concentrations.

The invention provides preparation of an electrochemical sensor based on a copper porphyrin base covalence organic frame material. Dispensing parts are adopted for modifying a bare electrode through carboxylic carbon nano-tubes, the copper porphyrin base covalence organic frame material is modified through a circular voltmeter-ammeter method, then, a constant potential deposition method is used for depositing metal cobalt nano-particles CoNPs on the surface of the modified electrode, the obtained modified electrode is the electrochemical sensor MWCNTs-COOH / CuP-SQ COFs / CoNPs / GCE based on the copper porphyrin base covalence organic frame material. The electrochemical sensor is adopted as a working electrode, a platinum electrode is adopted as a counter electrode, a saturated calomel electrode is adopted as a reference electrode, and a differential pulse voltmeter-ammeter method is adopted for simultaneously detecting guanine and adenine. The constructed electrochemical sensor has the advantages of being high in sensitivity, low in detection limit, wide in linear range, good in stability, high in interference resistance and the like.

The invention discloses a preparation method of an electrode for detection of dopamine (DA). The preparation method of the electrode comprises the following steps: dispensing reduction graphene oxide (rGO) onto the screen-printed electrode (SPE) surface to obtain SPE / rGO, using a cyclic voltammetry method for electrolytic deposition of a beta-cyclodextrin (beta-CD) and glucose oxidase (GOx) mixture onto the SPE / rGO electrode to obtain an modified electrode SPE / rGO / CD / GOx. The preparation method has the advantages of low cost and simple steps, and the prepared electrode is fast in response speed, high in sensitivity and good in selectivity.

A nucleotide triphosphate (NTP) participates in a phosphorylation reaction, wherein a phosphate group is transferred from the NTP to a substrate by a kinase. Provision in a kinase reaction of a NTP whose gamma phosphate is conjugated to an electroactive label results in the transfer of the gamma phosphate-electroactive label conjugate from the NTP to the substrate. The electroactive label is an organic moiety such as a quinone or a mtrohetercycle, or is a metallocene such as a ferrocene or a cobaltocene. Upon transfer of the gamma phosphate-electroactive label conjugate to an electrode-bound substrate by a kinase, the phosphorylation event is detected electrochemically by cyclic voltammetry. Phosphorylation can also be detected by mass spectrometry of a substrate carrying the electroactive label-conjugated gamma phosphate. NTP comprising the gamma phosphate-electroactive label conjugate is used in methods of detecting the presence of a kinase in a sample, screening candidate compounds that modulate kinase activity, and in methods of diagnosing a disease associated with a kinase.

The invention provides a preparation method of a titanium surface polydopamine film, wherein the preparation method includes the steps: S1) adjusting the pH value of a dopamine solution to be faintly acid or neutral with a weak acid, to obtain a reaction solution; S2) providing a three-electrode system, taking a titanium substrate as a working electrode, and making one face of the titanium substrate contact with the reaction solution; and S3) connecting the three-electrode system with the voltage, and constructing the polydopamine film on the surface of the titanium substrate by a cyclic voltammetry method. Compared with the prior art, dopamine is polymerized and deposited on titanium surface by an electrochemical method to form a uniform and smooth polydopamine film layer which resists strong acids and strong alkalis, dopamine can be allowed to be directly subjected to redox reaction to form more stable covalent bonding, the redox process of dopamine polymerization can be effectively controlled, and the polydopamine film constructed on the titanium surface is rich in phenolic hydroxyl groups and is dense and uniform, and has the advantages of better ionic permselectivity, good hydrophilicity and good oxidation resistance.

The invention relates to the technical field of biosensors and discloses an estriol hapten and antibody and the application thereof in a fast sensor method for uncojugated estriol detection. The structure of the hapten is scientifically designed, so that sensitivity and specificity of the antigen-antibody are improved; electro-deposition of a prussian blue-chitosan-nanogold composite membrane on the surface of an electrode is conducted, the estriol antigen is fixed to the surface of the membrane, a novel horse radish peroxidase labeled estriol antibody and a sample to be tested are added after an unbound site is sealed, the sample and a fixed antigen compete for a free enzyme labeled antibody, hydrogen peroxide is added after washing, a current signal is measured, a cyclic voltammetry estriol standard curve is obtained, the fast sensor method for uncojugated estriol detection is provided successfully, detection range is 0.001-100 ng / mL, and detection limit is 0.001 ng / mL. The method has the advantages of being easy to operate, high in sensitivity, quick, convenient and the like and can be well applied to ultra-sensitive and fast detection of estriol in the sample.

The invention discloses a Pt-CeO2 modified glassy carbon electrode and its application. According to the electrode, a glassy carbon electrode is used as a carrier, CeO2 is used as a cocatalyst, and Pt is used as an active component. The electrode is prepared by the following preparation step: chloroplatinic acid and a cerous nitrate solution are used as a precursor solution, and codeposition of Pt and CeO2 is carried out on the surface of the polished glassy carbon electrode within an appropriate voltage range by the utilization of a three-electrode system by a cyclic voltammetry method so as to prepare the Pt-CeO2 / GC electrode. The preparation of the electrode requires no addition of a surfactant, is easy to control, and has advantages of mild condition and simple technology. The prepared Pt-CeO2 modified glassy carbon electrode has excellent catalytic activity to ammonia nitrogen in an alkali system. The catalytic activity of the loaded Pt per unit mass to ammonia nitrogen is 0.975mC / microgram. Electrochemical active area of Pt per unit mass is 0.396cm<2> / microgram.

The invention provides a method for determining electrode reaction parameters. The method comprises the following steps: accessing three electrodes of a three-electrode system reaction device to an electrochemical workstation, measuring initial data by utilizing a cyclic voltammetry, processing the initial data to obtain electrochemical reaction parameters and data relationship graph; processing the initial data, namely, firstly reading files in batch, storing the read data by adopting a four-layer data structure, reading the information of temperature and scanning rate from a file name, correcting and preprocessing the data after all data is read and stored, drawing according to a result after the correction, judging the reaction type, and combining a computational formula to obtain the diffusion coefficient, reaction activating energy and rate constant data, and finally displaying the calculation result and graph. By adopting the method, the reaction kinetics and the thermodynamics parameters of the electrode can be conveniently measured, the determination period is greatly shortened, the overall workload is alleviated, the overall price is low, and the final calculation result and drawn graph can meet the majority of requirements.

The invention relates to the technical field of glucose detection, and particularly relates to an enzyme-free glucose sensor, a production method and an application thereof. Magnolia grandiflora leaves are used as a carbon-based catalyst, nickel atoms are well dispersed when the magnolia grandiflora leaves are used as a substrate material, and the catalytic activity of the material is improved. And the prepared Ni@ NSiC nano molecular layer is modified on a pretreated white glassy carbon electrode to obtain a high-activity modified working electrode Ni@ NSiC / GCE, and glucose is detected by adopting a cyclic voltammetry and a chronoamperometry. The method solves a problem that dopamine, ascorbic acid, uric acid and other easily oxidized interfering substances exist when the glucose is detected through a traditional electrooxidation method. Test results show that the material has excellent electrochemical sensing performance on the glucose, has relatively high sensitivity, a good stability and anti-interference capability, and has a wide application prospect in the fields of electro-catalysis and biosensing.