The invention relates to a preparation method of a glucose sensor, and in particular to a preparation method of a non-
enzyme electrochemical glucose sensor, aiming at solving the problem that the existing glucose sensor cannot carry out non-
enzyme electrochemical
glucose detection under the neutral and partial neutral conditions. The method comprises the steps of 1,
grinding and cleaning an
electrode; 2, detecting the performance of the
electrode; 3, synthesizing: dissolving Co(NO3)2 6H2O in methyl
alcohol, and stirring for dissolving to obtain a solution A; dissolving dimethyl
imidazole in the methyl
alcohol, and stirring for dissolving to obtain a solution B; then, mixing the solution A with the solution B, and standing; 4. cleaning and
drying to obtain a
metal-organic framework material ZIF-67; 5, carrying out heat treatment; 6, adding black
powder into a
solvent to obtain suspension liquid, carrying out ultrasonic treatment on the suspension liquid, adding the treated suspension liquid onto the surface of the
glassy carbon electrode drop by drop, and
drying to obtain the non-
enzyme electrochemical glucose sensor. The non-enzyme electrochemical glucose sensor can carry out non-enzyme electrochemical
glucose detection under the neutral and partial neutral conditions, and is simple and practicable as well as low in cost. The non-enzyme electrochemical glucose sensor is used for detecting glucose.