The invention relates to a method for preparing a
graphene biosensor, belonging to the technical field of
electrochemistry. The method comprises the following steps of: immersing a processed gold
electrode into
graphene oxide and a
sodium sulphate solution, electrically depositing the
electrode through a control
electric potential, taking out the
electrode, washing the electrode by using water, after
drying the electrode at
room temperature, putting the electrode in a
chloroauric acid solution, electrically depositing the electrode through the control
electric potential, taking out the electrode, washing the electrode by using water, and
drying the electrode at
room temperature; putting a modified electrode in a
conductive polymer monomer and a
supporting electrolyte solution, polymerizing the electrode through the control
electric potential by adopting a
cyclic voltammetry, taking out the electrode, washing the electrode by using water, and
drying the electrode at
room temperature; and activating the modified electrode in an EDC / NHS (
Dichloroethane / N-Hydroxysuccinimide) solution, and immersing the modified electrode in a
vomiting toxin antibody. The method disclosed by the invention is used for fixing
graphene, gold nanoparticles and conducting polymers through electro-deposition; therefore, the method is very green and environment-friendly; furthermore, the thickness of a
coating and sizes and distribution densities of the gold nanoparticles can be precisely controlled, thus, the
batch production repeatability of the modified electrode is good.