1929 results about "Molecular imprinting" patented technology

The present invention relates to molecularly imprinted polymers comprising tailor-made recognition sites for a target in which said recognition sites are located at or close to the surface of the polymer and / or of pores in the polymer. The molecularly imprinted polymer comprising tailor-made recognition sites for a target is obtainable by polymerising functional monomers and, optionally, cross-linked, optionally in a reaction solvent, in the presence of at least one template immobilised on a support material in a polymerisation process, whereby non-covalent or covalent are formed between said functional monomers and said immobilised template(s), and removing said template(s), and said support material from the molecularly imprinted polymer.

Relating to sensors and molecular imprinting technologies, the invention discloses an electropolymerization molecular imprinting technology-based double-parameter composite micro-sensor and a preparation thereof. According to the invention, three electrochemical microelectrode systems are integrated on a same chip, and each electrochemical microelectrode system has its independent micro-electrochemical reaction pool. Through encapsulation by a sealant, the integrated chip can form an open composite measurement pool containing three electrochemical microelectrode systems. In the micro-reaction pool of each electrochemical microelectrode system, by injecting a solution from the outside, a molecular imprinting procedure containing in situ polymerization and ultrasonic elution of template molecules can be implemented separately, thus obtaining a molecularly imprinted sensor. The left and right microelectrode systems of the composite micro-sensor respectively recognize two corresponding molecules due to different molecularly imprinted sensitive membranes, and the electrochemical microelectrode system positioned in the middle is used as a differential detection reference so as to deduct background signals and environmental effects of a test system. The composite micro-sensor provided in the invention can recognize two molecules simultaneously.

The invention relates to a molecular imprinting chemiluminescence sensor for detecting trace amount pesticide residues, belonging to the technical field of the detection of the pesticide residues. Theinvention also relates to a method for detecting the trace amount pesticide residues contained in agricultural product samples by using the molecular imprinting chemiluminescence sensor, which comprises the following steps: (1) selecting a functional monomer; (2) uniformly mixing a template molecule, the functional monomer, a cross linker, a pore-forming agent, an initiating agent and a silicon source to prepare an MIPs collosol according to a mole ratio of 0.1-1 to 1-10 to 5-25 to 20-60 to 0.05-0.15 to 20-50; (3) preparing a quantum dot material solution; and (4) modifying the MIPs collosoland quantum dot materials to the surface of a millipore plate to manufacture the chemiluminescence sensor by a layer-layer self-assembly surface modification technique. The invention has the advantages of high specificity and sensitivity, fast detection, low cost, good repeatability and convenient field detection on the detection of the pesticide residues.

The invention discloses a method for preparing bisphenol A molecular imprinting polymer, comprising the following procedures: dissolving template molecule bisphenol A and functional monomer according to the mol ratio of 1.5:1 to 2:1 into acetonitrile solution which is no less than 10ml with 10 to 15 crosslinking agent and initiator of catalytic amount to prepare mixed liquor; the mixture liquor is churned up at 55 to 70 DEG C to polymerize for 12 to 24 hours and then white powdery polymer is generated; synthesizing fine white powdery polymer using mixed solution of methanol and acetic acid as extracting solution, removing the template molecules and then extracting acetic acid molecules using pure methanol; after extracting, the polymer is arranged in vacuum drying chamber to dry until reaching balancing weight and then bisphenol A molecular imprinting polymer is obtained. The invention has advantages that the prepared bisphenol A molecular imprinting polymer has special adsorption for bisphenol A molecular in cosmetic samples which can rapidly, delicately and accurately concentrate and purify the cosmetic samples in an efficient way; the rate of recovery is high, the reproducibility is good and the sensitivity is high.

The invention relates to a preparation method of a protein magnetic imprinting nano sphere, in particular to a preparation method of a protein magnetic imprinting nano sphere which takes one of hemoglobin, muramidase or serum protein as template molecules; the preparation method includes the following steps: synthesizing magnetic nano particles which are Fe3O4 nano particles covered with silicon dioxide on the surfaces; carrying out silanization to the amino-groups on the surface of the magnetic sphere; using glutaric dialdehyde to connect protein; using silylation agent to fix the space structure of template protein; alkaline eluting the protein on the surface of the magnetic sphere to form imprinting sites. The hemoglobin, muramidase or serum protein magnetic nano imprinting polymer sphere prepared by the invention combines the precise and specific identification property of molecular imprinting and the quick separation property of the magnetic nano sphere under the action of an external magnetic field, integrates the advantages of molecular imprinting and the magnetic nano sphere, avoids complex centrifugation operation, plays vital roles in the separation of cells, protein and nucleic acid, the detection of biomolecules, tumor diagnosis and medicine targeting therapy and has promising application prospect in the field of isolation analysis.

The invention discloses a method for detecting an electrode of a molecular imprinting membrane of EDCs and the EDCs; the preparing method of the molecular imprinting membrane electrode comprises the following steps: a functional monomer is selected; template molecule of the EDCs, the functional monomer, a crosslinker, a porosity-making agent, an initiating agent and organic solvent are mixed evenly according to a certain molar ratio so as to prepare MIPs solution; nanometer material solution is prepared; the nanometer material and molecular imprinting polymer MIPs are modified on the surface of the electrode of a sensor by using the electrode surface modifying technology; a method for detecting trace amount of the EDCs comprises the following steps: the electrode of the molecular imprinting membrane is connected with an electrochemical working station and detects the EDCs in the environment sample extract; the electrode of the molecular imprinting membrane of the invention has strong specificity and high sensitivity which can reach ng level; a basic detection process is completed by only 1 to 2 minutes; the cost is low and the detection of one sample needs only a plurality of cents; the method for detecting the secretion in the environment by the electrode of the molecular imprinting membrane has fast and simple operation and the reaction and result are completed and recorded automatically by instruments.

The invention discloses a molecularly imprinted magnetic microsphere, a preparation method and an application thereof. The preparation method comprises the following steps: S1, adopting a coprecipitation method to prepare Fe3O4 nanometer particles; S2, adopting a microemulsion polymerization method to prepare a magnetic composite microsphere by virtue of the Fe3O4 nanometer particles; S3, adopting a sol-gel method to grow SiO2 on the surface of the magnetic composite microsphere to obtain a core-shell microsphere; S4, grafting C=C double bond on the surface of the core-shell magnetic microsphere; and S5, adopting a substitution template method on the surface, which is grafted with the C=C double bond, of the core-shell microsphere to carry out molecular imprinting operation to obtain the molecularly imprinted magnetic microsphere. DMIP (Dimethyl Isophthalate) prepared by the method provided by the invention has excellent superparamagnetism and excellent selectivity for target molecules.

The invention discloses a device and a method for the combined use of molecular imprinting solid phase microextraction and hollow fiber liquid phase microextraction, and the application thereof. The device consists of a solid phase microextraction device, a molecular imprinting solid phase microextraction probe, a hollow fiber membrane, an extraction bottle and a stirrer. The molecular imprintingsolid phase microextraction probe is inserted in to a hollow fiber membrane cavity containing organic solvent, fixed and then directly positioned into a sample solution to be measured for purpose of liquid-solid phase synchronous microextraction. During the microextraction, analytes permeate the hollow fiber membrane, are then enriched in the organic solvent and then undergo secondary extraction and enrichment under the action of the molecular imprinting solid phase microextraction probe. The device is simple, operates conveniently, facilitates the combined use with an analyzer, is suitable for the separation and enrichment of organics with trace amounts in complex water phase samples obtained from the fields such as environment, food, medicine, biology and the like.

The invention discloses a method for preparing a molecularly imprinted TiO2 / WO3 composite photocatalyst with visible light response through a direct method. The method is as follows: a titanium alkoxide is used as a sol and functional monomer precursor, an organic pollutant is used as a template molecule, absolute alcohol is used as a solvent, glacial acetic acid is used as an inhibitor, a sol-gel method is adopted, and the materials are prepared according to a certain ratio, wherein the molar ratio of the template molecule to Ti is 1:20-1:10, and the titanium alkoxide, absolute alcohol, glacial acetic acid and water are mixed in a volume ratio of 10:19:8:4. The method has the following technical effect: the titanium alkoxide has double functions, namely the titanium alkoxide can be used as a titanium source and can also be used as a functional monomer in the hydrolysis process. Other organic functional monomers are not required.

The invention relates to a molecular imprinting photonic crystal for detecting glucose, which belongs to the technical field of applied chemistry and clinical analysis test. A method comprises the following steps: firstly, inserting a substrate subjected to hydrophilic treatment into PMMA colloidal pellet solution, and self-assembling pellets on the substrate to obtain a three-dimensional photonic crystal template; secondly, dripping molecular imprinting pre-polymerization solution on the edge of the three-dimensional photonic crystal template, and thermally polymerizing the template; and finally, cleaning the glucose imprinting template to obtain a glucose molecular imprinting photonic crystal template. The molecular imprinting photonic crystal has specific glucose adsorption performance, enables direct observation of the change, and fulfills the aim of real-time, quick and convenient detection.

The invention provides a preparation method for a molecular imprinting material and the molecular imprinting material prepared through the preparation method. The preparation method comprises the steps that silicon oxide is coated on the surfaces of magnetic ferroferric oxide nanometer particles, the magnetic ferroferric oxide nanometer particles are modified with gamma-(methacryloyl chloride) amino propyl trimethoxy silane to obtain magnetic ferroferric oxide nanometer particles with propenyl on the surfaces, the magnetic ferroferric oxide nanometer particles with the propenyl on the surfaces serve as carriers, estrogen receptors are simulated, functional monomers are optimized, a surface imprinting technology is adopted, and then the molecular imprinting material which can simultaneously identify seven kinds of environmental endocrine disrupting chemicals is prepared. According to the preparation method, the easy separation of a magnetic nanometer material, the good water solubility of a silicon oxide nanometer material, the specific recognition ability of molecular imprinting polymers and the surface imprinting technology are mutually combined, the preparation technology is simple, the conditions are mild, the prepared molecular imprinting material is large in adsorption capacity, fast to respond, high in magnetism, good in chemical stability and high in repeating utilization rate, and the problems that at present, multiple trace, steroid and phenol environmental endocrine disrupting chemicals are difficult to simultaneously identify, separate and enrich are solved.

Surface-molecularly imprinted sensors were fabricated for detecting ionic molecules. Target molecules are recognized by the combination of a hydrophobic interaction with the imprinted polymer layer to provide specificity and an electrostatic interaction to provide sensitivity Coupling surface imprinting techniques with an electrochemical detection method, such as potentiometry, allows specific recognition of target molecules and translation of the recognition event into an output signal by the sensor.

The invention provides a preparation of a bisphenol A submicron magnetic molecular imprint and application of the same in the detection pre-processing of packaged food, which belong to the technical field of material chemistry and food safety. A surface molecular imprinting technology and a magnetic solid-phase extraction technology are combined, and a magnetic submicron particle wrapped by a surface molecular imprint polymer (Fe3O4@MIP) is prepared by using a bisphenol A as a template molecule. A magnetic submicron material (Fe3O4) is synthesized by a hydrothermal process, the synthesized Fe3O4 is synthesized into a silicon-wrapped magnetic microsphere (Fe3O4@SiO2) by a sol gel process, the synthesized Fe3O4@SiO2 is coupled with 2-bromo-isobutyryl bromide to form Fe3O4@SiO2@Br with Br groups on the surface, and the synthesized Fe3O4@SiO2@Br is synthesized into a submicron particle with a surface molecular imprint (Fe3O4@MIP) by an atom radical transfer process. The prepared Fe3O4@MIP is used in the detection pre-processing of packaged food which comprises liquid food, liquid food containing a certain quantity of solids and solid food.

The invention discloses a quantum dot molecular imprinting microsphere quartz fluorescent sensor for detecting multicomponent food additives simultaneously and a method for detecting the food additives by the quantum dot molecular imprinting microsphere quartz fluorescent sensor. A method for preparing a quantum dot molecular imprinting microsphere comb quartz plate comprises the following steps of: selecting functional monomers corresponding to the food additives; preparing quantum dots and preparing quantum dot molecular imprinting microspheres according to the literature; and modifying thesurfaces of different probes of the comb quartz plate with the imprinting microspheres of different food additives by utilizing layer-upon-layer accumulated surface modification technology. The method for detecting the trace multicomponent food additives simultaneously (which is shown as the figure) comprises the following steps of: immersing the modified quartz plate into simply-pulpified food solution, arranging the quartz plate on a sealing quartz vessel, and detecting the food additives in a sample. The quantum dot molecular imprinting microsphere quartz fluorescent sensor has high specificity and sensitivity, short detection time and low cost; and in a method for detecting pesticide residues by fluorescent light, the operation is simple and quick, and reactions and results are completed and recorded automatically by instruments.

The invention belongs to the technical field of sample pretreatment in analytical chemistry and relates to a preparation method and use of a molecular imprinting-absorbing extraction stirring rod. The preparation process of the method comprises the following steps: sintering one end of a capillary glass tube serving as a substrate of the stirring rod, and subjecting to the sintered capillary glass tube to acid and alkali treatment and surface modification by a silylating agent respectively; performing the self-assembly of template molecules and bifunctional monomers in a polymer solvent, adding a crosslinker and an initiator in the solution, plugging the modified capillary glass tube into the solution; drawing the capillary glass tube out; repeatedly coating the capillary glass tube to a required thickness, and aging the coating; cutting a proper length of coated capillary glass tube, putting the length of coated capillary glass tube in a magnetic core, and sintering the capillary glass tube for end sealing; and eluting the template molecules. The molecular imprinting-absorbing extraction stirring rod prepared by the invention can selectively separate and enrich a trace amount of template molecules and analogues at the same time and is suitable for selectively separating, enriching and analyzing a trace amount of template molecules and analogues in complex samples in terms of organism, food and environment.

The invention relates to a composite functional membrane with adsorption, catalytic purification and separation functions for removing pollutants from water, belonging to the technology field of environment and chemical engineering. The method comprises the steps of doping titanium oxide and other coating separation membranes in adsorbing materials (carbon materials, inorganic materials and molecular imprinting materials to obtain a composite functional membrane with adsorption, catalytic purification and separation functions; and adsorbing pollutants with the membrane, separating, oxidizing and mineralizing the absorbed pollutants to regenerate the purification and adsorption functions of the membrane. The inventive method improves pollutant adsorption and catalytic oxidation efficiency; and preventes the pollution of oxidized byproducts to the quality of effluent water after membrane separation, so as to be safer when being used for drinking water purification. The inventive method has the advantages of high efficiency, complete removal of pollutants, low pollution and low energy consumption; and can be used for purifying water bodies such as slightly polluted water, recycled water or water containing low-concentration trace organic pollutants.

The invention relates to a preparation method of a magnetic functionalized graphene oxide based molecularly imprinted hybrid material. The preparation method comprises the following steps: firstly, loading Fe3O4 magnetic nanoparticles on the surface of graphene oxide by chloroformylation reaction; and synthesizing the magnetic functionalized graphene oxide based molecularly imprinted hybrid material on the surface of the magnetic functionalized graphene oxide serving as a carrier by utilizing a molecular imprinting technology. The prepared magnetic functionalized graphene oxide based molecularly imprinted hybrid material can be used for selectively removing estrogen in drinking water. The preparation method of the magnetic functionalized graphene oxide based molecularly imprinted hybrid material has the advantages that the process is reasonable and easy to implement; when being used for removing endocrine disruptors, the material has good chemical stability and reusability, and active adsorption, identification and separation of the estrogen are easily carried out; and the preparation method has strong magnetic response, preordainment, identity and practicality, and can greatly improve the using efficiency of the molecular imprinting technology and expand the range of molecular imprinting applications.

The invention discloses molecular imprinting polymer which belongs to the technical field of pesticide molecular imprinting and preparation method thereof. The molecular imprinting polymer is prepared by the following steps of: mixing a polymerization reaction monomer, a cross-linking agent, a hole inducing agent, an initiating agent and an imprinting molecule diethyl(3-methyluren)(phenyl)methyl phosphonate, carrying out in-situ polymerization reaction under the condition of thermal initiation and / or photo-initiation to obtain a polymer containing diethyl(3-methyluren)(phenyl)methyl phosphonate; washing the polymer till an imprinting molecule is not detected; and then washing by an organic solvent to neutrality and drying in vacuum to obtain the molecular imprinting polymer. The invention also discloses applications of the molecular imprinting polymer in aspects of separating, purifying and detecting organophosphorus pesticide in a sample. The applications of the polymer prevent the influence of target analyte template leakage on a following adsorption experiment, can simultaneously detect various target molecules and widen the detecting range of the target molecules.

The invention provides a method for preparing lysozyme molecular imprinting nano particles with magnetic responsiveness and extremely high adsorption capacity. In the method, lysozymes are used as template molecules. The method comprises the following steps of: carrying out template polymerization under the conditions that suitable functional monomers, a cross-linking agent and an initiator and magnetic nano particles the surfaces which are coated with SiO2 and are modified by sulfydryl and acryloyloxy exist; and washing out the template molecules to obtain the lysozyme molecular imprinting nano particles with magnetic responsiveness and high adsorption capacity. The invention also provides a method for preparing the nano particles. The lysozyme molecular imprinting nano particles prepared by adopting the method disclosed by the invention have the characteristics of high adsorption capacity, simpleness for preparation, low cost, strong specific absorption, high physical, chemical stability, capability of being recycled repeatedly and the like.

A molecular imprinting polymer of a pyrethroid insecticide is characterized in that the polymer is prepared by the following steps: a. a pyrethroid template molecule, a functional monomer for polymerization, a cross-linker and a pore-forming agent are mixed; b. the substances are arranged in a closed container after being uniformly mixed, the pure nitrogen aeration is used for removing the oxygen, the in-situ polymerization is carried out by adopting the thermal-initiation or the photo-initiation mode after the closing, thus generating the molecular imprinting polymer; c. the molecular imprinting polymer is ground, mixed liquid of organic solvent and acid is utilized for removing the template molecule; d. and the molecular imprinting polymer which is obtained in step c is dried in the vacuum condition. The invention further discloses an application of the polymer, compared with the prior art, the molecular imprinting polymer has the advantages that: the preparation process is simple, the operatability is strong and the preparation cost is low; the prepared polymer has good selectivity and high enrichment degree, which can be widely applied in the pre-treatment process of the pyrethroid insecticide residues of the environmental, biological and food samples, etc.

The invention relates to a preparation method of a bionic artemisinin molecular imprinting composite membrane, belonging to the technical field of environmental material preparation. The preparation method comprises the following specific steps: by taking a regenerated cellulose membrane as a substrate, artemisinin as a template molecule, acrylamide (AM) as a functional monomer and ethylene glycol dimethacrylate (EGDMA) as a cross-linking agent, inlaying silicon dioxide nanoparticles with functional monomer modified surfaces to the surface of a membrane material by combination with a dopamine bionic principle, and performing two-step temperature polymerization to prepare the bionic artemisinin molecular imprinting composite membrane. The static adsorption experiment is used for making a research on the adsorption balance, dynamics and selective recognition performance of the prepared imprinting membrane. A result shows that the artemisinin imprinting membrane prepared by adopting the method is relatively high in adsorption capacity and superior in artemisinin molecular recognition penetration performance.

The invention discloses a molecular imprinting electroluminescent sensor for detecting veterinary drug residue by taking a battery as power and a method for detecting veterinary drug residue. A preparation method of an electrode (a schematic diagram is shown in the figure) comprises the following steps of: preparing MIP (Molecularly Imprinted Polymer)s sol of the veterinary drug residue; preparing a carbon dot and preparing a graphene nano material according to documents; and modifying graphene, the carbon dot and the MIPs sol onto the surface of the electrode of the sensor. The method for detecting the trace veterinary drug residue comprises the following steps of: connecting the modified electrode to an electrogenerated chemiluminescence instrument, and detecting the veterinary drug residue in a sample extract by taking the battery as the power. The molecular imprinting electroluminescent sensor disclosed by the invention has the advantages that the specificity of the electrode is strong and the sensitivity is high and can be up to a ng grade; only 3-5 minutes are spent on a basic detection process; and the cost is low. The method for detecting the veterinary drug residue by adopting the electrode is quick and easy to operate, the reaction is automatically completed by instruments and results are automatically recorded by the instruments.

The invention provides a preparation method and use of a graphene oxide surface molecularly imprinted sol-gel polymer and relates to the technical field of combination of polymer material science and chemical sensors. Graphene oxide as a carrier and siloxane as a functional monomer and a cross-linking agent are subjected to sol-gel method treatment in an aqueous solution at a normal temperature so that the graphene oxide surface molecularly imprinted sol-gel polymer is obtained, and the graphene oxide surface molecularly imprinted sol-gel polymer can be used in the field of electrochemical sensors and can realize fast analysis detection of a template molecule. The preparation method has the simple process of mixing the carrier, the template molecule, the functional monomer, the cross-linking agent and the solvent according to a certain ratio, has a low cost, saves energy and is environmentally friendly. The graphene oxide surface molecularly imprinted sol-gel polymer-based electrochemical sensor has the advantages of rapid response to the imprinted molecule, good selectivity, high sensitivity and good stability.

The invention discloses a preparation method of a lysozyme molecular imprinting-quantum dot nanoscale fluorescent probe. The preparation method adopts quantum dots as carriers and lysozyme as a template molecule and modifies a molecular imprinting polymer layer on surfaces of the quantum dots to synthesize the novel lysozyme molecular imprinting-quantum dot nanoscale fluorescent probe through combining selectivity of a molecular imprinting technology and fluorescent characteristics of the quantum dots. Specially, the preparation method comprises the following steps of preparing quantum dots, modifying surfaces of the quantum dots by denatured bovine serum albumin, forming a molecular imprinting layer through a polymerization reaction initiated by a selected functional monomer and cross-linking reagents, and washing away the template molecule by selected eluent. The lysozyme molecular imprinting-quantum dot nanoscale fluorescent probe can combine accuracy and specific identification characteristics of molecular imprinting and high sensitivity characteristics of quantum dot fluorescence detection and has high selectivity and sensitivity of template molecule identification. The preparation method has simple processes, good reproducibility between different batches, good application prospects in selective identification and detection of lysozyme in an actual sample.

The invention discloses a photoelectric chemical analysis method adopting a molecular imprinting functionalization modified electrode, relates to a molecular imprinting technology-based preparation method of a high-selectivity TiO2NTs modified electrode, and also relates to a method for high-selectivity and high-sensitivity detection of endocrine disrupting chemicals especially such as 2,4-dichlorophenoxyacetic acid (2,4-D) having no electrochemical activity by the high-selectivity TiO2NTs modified electrode as a photo anode. Compared with the prior art, the molecular imprinting technology-based preparation method creatively utilizes a molecularly imprinted polymer in selective modification of a photoelectric sensing electrode so that selectivity and detection sensitivity of an electrode are improved greatly; the interference caused by multiple concomitant micro-molecules is eliminated; a magnitude of a detection limit reaches to 10<-8>mol / L<-1>; the molecular imprinting functionalization modified electrode is stable; and the photoelectric chemical analysis method for detection has good reappearance.

The invention provides a magnetic molecularly imprinted nano-particle as well as a preparation method and an application thereof. The nano-particle can perform specific recognition, trapping, separation and activity inhibition on target protein in a solution in vitro, more importantly, the nano-particle can enter a living cell rapidly under the action of a magnetic field and perform in-situ combination and activity inhibition on the target protein in the living cell, distribution of the nano-particles in the cell can be traced through a fluorescence microscope after fluorescence labeling, and quantitation can be performed through detection of fluorescence intensity.

The invention discloses a preparation method of a GR / WS2-AuNPs-WS2 compound molecular imprinting sensor. The preparation method includes the steps: 1) preparing tungsten sulfide which is nanosheet transition metal; 2) preparing an AuNPs / WS2 compound; 3) preparing a GR / WS2-AuNPs-WS2 compound; 3) preparing a GR / WS2-AuNPs-WS2 compound modified electrode; 4) taking the GR / WS2-AuNPs-WS2 compound as a substrate of the modified electrode, L-phenylalanine as a template molecule, 2-ethyl-4-methylimidazole as a crosslinking agent and methacrylic acid as a functional monomer to prepare an L-phenylalanine molecular imprinting sensor. The invention further provides an application of the molecular imprinting sensor in detection and separation of L-phenylalanine. L-phenylalanine detection sensitivity of the sensor is greatly improved owing to the GR / WS2-AuNPs-WS2 compound, and the prepared L-phenylalanine molecular imprinting sensor is high in L-phenylalanine sensitivity and selectivity by taking advantages of molecular imprinting.

The invention provides a preparation method for a print adsorbent of chitosan molecules, which relates to a preparation method for an adsorbent that can selectively remove perfluorooctane sulfonate (PFOS) in water. The preparation method adopts a molecular imprinting technique to construct a plurality of specific adsorption sites and the worthy adsorbent has remarkable selectivity adsorption to the PFOS in water. The method takes chitosan as raw material to prepare micro-cross linking chitosan beads; then the chitosan beads are printed with template molecules PFOS for 5 to 24 hours in an acid water solution, a mass ratio between the template molecules and the micro-cross linking chitosan beads is 0.005 to 0.06: 1, and reaction with a cross linker is carried out for 2 to12 hours after printing; the mass ratio between the volume of the cross linker and the micro-cross linking chitosan beads after being printed is 0.0125 to 0.119ml / g; finally, an organic solvent is used for eluting for 1 to 8 hours and the print adsorbent of chitosan molecules is obtained after freeze-drying. The adsorbent is suitable for adsorbing and removing PFOS in complicated water substances and is characterized by strong selectivity and being able to be reused.

The invention provides an endocrine interferent molecularly imprinted membrane substrate in a trace environment with rapid detection speed and high sensitivity, the preparation method and the application thereof. The molecularly imprinted membrane substrate comprises a gold quartz crystal substrate, and multi-layer nano-materials that alternate with each other and environmental endocrine interferent molecularly imprinted polymer are formed on the surface of the gold quartz crystal substrate. The preparation method comprises the following steps: selecting functional monomer that can be synthesized with the environmental endocrine interferent into the molecularly imprinted polymer; preparing the molecularly imprinted polymer solution; and modifying the nano-material and the molecularly imprinted polymer on the surface of the gold quartz crystal substrate. The invention applies the surface modification technology to the preparation of the molecularly imprinted membrane substrate, so as to allow the controllability over the preparation of the nano-scale synergetic EDCs molecularly imprinted membrane substrate. A method for detecting the environmental endocrine is rapid and simple, and the reaction and the result are automatically recorded and completed by an instrument with good repeatability, thereby facilitating the on-site detection.

The invention discloses a preparation method of molecularly imprinted polymeric microspheres for 2,4,6-trinitrophenol detection. The preparation method is characterized in that polynitrophenol derivatives-imprinted molecules generated through reaction between 2,4,6-trinitrophenol and N,N'-diisopropyl carbodiimide have luminous yellow fluorescent light, the fluorescent light of the imprinted molecules has open and close characteristics under an acid-base condition, the imprinted molecules located in an imprinting layer of the molecularly imprinted polymeric microspheres can be eluted, a hole structure which is complementary with the structure, the size and the functional groups of the imprinted molecules is formed in the imprinting layer, polymeric microspheres of which the imprinted molecules are eluted have specific recognition sites on target analyte molecules, target molecules enter the specific recognition sites, selective recognition on the target analyte molecules is realized, sensitivity detection of the target analyte molecules can be realized through opening and closing of the fluorescent light of the target molecules, the specific surface area of the molecularly imprintedmicrospheres is large, and space in which the recognition sites are formed is stable in structure, has more effective sites, and is capable of carrying out high-selective recognition and high-sensitive detection on the target molecules.