In the method for efficiently extracting endocrine disruptors in a sample of the present invention, the sample to be tested is preliminarily treated by filtering,
grinding, homogenizing, ultrasonication,
protein precipitation,
protein hydrolysis or
degreasing to make a sample solution of an aqueous matrix; adjust the pH value of the sample solution, Or add
inorganic salts or organic salts to increase the
ionic strength of the sample solution to reduce the distribution coefficient of endocrine disruptors in the water phase; use the membrane disc
solid phase extraction device to make the sample
solution flow continuously or "flow-stop-flow" intermittent Through the activated nylon
nanofiber membrane, the endocrine disruptors in the sample are retained by the nylon
nanofiber to achieve extraction. The invention synergizes the advantages of
solid-phase membrane extraction and nano-adsorption media, and can realize efficient extraction of internally distributed interfering substances in various actual samples with only a few milligrams of nylon
nanofiber membrane and hundreds of microliters of
elution solvent , The extraction and enrichment process of separation, adsorption, concentration and
elution can be completed within a few minutes to ten minutes.