2537 results about "Offspring" patented technology

An improved method of nuclear transfer involving the transplantation of donor differentiated cell nuclei into enucleated oocytes of the same species as the donor cell is provided. The resultant nuclear transfer units are useful for multiplication of genotypes and transgenic genotypes by the production of fetuses and offspring, and for production of isogenic CICM cells, including human isogenic embryonic or stem cells. Production of genetically engineered or transgenic mammalian embryos, fetuses and offspring is facilitated by the present method since the differentiated cell source of the donor nuclei can be genetically modified and clonally propagated.

Mice are provided that comprise a reduction or deletion of ADAM6 activity from an endogenous ADAM6 locus, or that lack an endogenous locus encoding a mouse ADAM6 protein, wherein the mice comprise a sequence encoding an ADAM6 or ortholog or homolog or fragment thereof that is functional in a male mouse. In one embodiment, the sequence is an ectopic ADAM6 sequence or a sequence that confers upon a male mouse the ability to generate offspring by mating. Mice and cells with genetically modified immunoglobulin heavy chain loci that comprise an ectopic nucleotide sequence encoding a mouse ADAM6 or functional fragment or homolog or ortholog thereof are also provided.

An improved method of nuclear transfer involving the transplantation of donor differentiated pig cell nuclei into enucleated pig oocytes is provided. The resultant nuclear transfer units are useful for multiplication of genotypes and transgenic genotypes by the production of fetuses and offspring. Production of genetically engineered or transgenic pig embryos, fetuses and offspring is facilitated by the present method since the differentiated cell source of the donor nuclei can be genetically modified and clonally propagated.

The present invention discloses a novel method for identifying an individual who may be susceptible to develop a developmental disorder. In one particular example, an individual is identified who is genetically susceptible to becoming schizophrenic. In addition, the present invention discloses a novel method for identifying individuals who are genetically susceptible to have offspring with a developmental disorder. Methods of diagnosing, preventing and treating developmental disorders such as schizophrenia are also provided.

Methods and cell lines for cloning bovine embryos and offspring are provided. The resultant embryos or offspring are especially useful for the expression of desired heterologous DNAs.

A genealogical research and record keeping system and method for identifying commonalities in haplotypes and other genetic characteristics of two or more individual members of a biological sample. Chromosomal fragments identical by descent identify family ties between siblings, parents and children and ancestors and progeny across many generations. It is particularly useful in corroborating and improving the accuracy of genealogical data, and identifying previously unknown genetic relationships.

A method of propagating mammalian endodermally derived progenitors such as hepatic progenitors, their progeny, or mixtures thereof is developed which includes culturing mammalian progenitors, their progeny, or mixtures thereof on a layer of embryonic mammalian feeder cells in a culture medium. The culture medium can be supplemented with one or more hormones and other growth agents. These hormones and other growth agents can include insulin, dexamethasone, transferrin, nicotinamide, serum albumin, beta-mercaptoethanol, free fatty acid, glutamine, CUSO4, and H2SeO3. The culture medium can also include antibiotics. Importantly, the culture medium does not include serum. The invention includes means of inducing the differentiation of the progenitors to their adult fates such as the differentiation of hepatic progenitor cells to hepatocytes or biliary cells by adding, or excluding epidermal growth factor, respectively. The method of producing mammalian progenitors is useful in that the progenitors can be used subsequently in one or more of the following processes: identification of growth and differentiation factors, toxicological studies, drug development, antimicrobial studies, or the preparation of an extracorporeal organ such as a bioartificial liver.

An improved process of matching people with one another, for social and/or sexual purposes, based on the profiles of the participants' Class I and Class II MHC genes. The quality of this human matching is inversely proportional to the number of common alleles of said genetic profiles of the individuals. Higher quality matches of this type result in greater sexual attraction, more attractive physical odor, and offspring with more robust immune systems among matched participants.

The invention discloses a recombinant vector and a non-GMO gene editing plant screening method. An initial carrier in the recombinant vector is a CRISPR/Cas9 carrier which contains sgRNA gene, Cas9 gene and screening label gene and is used for plant gene editing, the recombinant vector carries a BelRNAi expression element, and a hairpin RNA interference fragment interfering with bentazone resistance gene is generated through transcription of the BelRNAi expression element. By introducing the BelRNAi expression element to the initial carrier and conducting bentazone herbicide screening on future generation plants, offspring with target gene mutated are reserved, and it is also ensured that the mutated offspring do not contain transgenic fragments; screening method is cheaper, simpler and more effective.

The invention provides a self-adaptive genetic particle swarm hybrid algorithm optimization method. The self-adaptive genetic particle swarm hybrid algorithm optimization method includes: calculatingthe density and the radius of a center region of a parent population in a genetic algorithm, and distinguishing whether the parent population is in the overall centralized distribution, the local centralized distribution or the uniform distribution; performing a selection operation of the genetic algorithm, and selecting a parent individual to be evolved; establishing computational formulas of thecrossover probability and the mutation probability according to the three distributions of the parent population; performing crossover and mutation operations according to the established crossover and mutation probability formulas so as to achieve chromosome recombination and gene mutation, and forming an offspring individual; selecting a part of individuals with high fitness from a part of offspring individuals to perform the particle swarm algorithm to form offspring particles, and combining the offspring individuals and the offspring particles into an offspring population and saving the optimal individual thereof. The invention adaptively adjusts crossover probability mutation probability parameter values in the genetic particle swarm hybrid algorithm, so that the convergence speed and the convergence precision are greatly improved.

The invention relates to a double resistance CRISPR/Cas9 carrier and application. The invention provides the double resistance carrier having both hygromycin B resistance and Basta resistance at the same time; the double resistance carrier is obtained by inserting sequences and fragments derived from multiple different carriers into a sequence of a carrier pFGC5941 by enzyme-cutting and linking up, and constructing. Hygromycin B is used for screening in a conversion process, and Basta is used for screening offspring transgenic plants not containing an external source T-DNA sequence in offspring plants. Two resistances, i.e., the hygromycin B and the Basta are used to respectively serve as screening marks of the transgenic plants for the first time, a feasible manner is provided for screening transgenic offspring not containing the external source T-DNA sequence, determining the existence of external source T-DNA by a complex molecular biology detection experiment is avoided, and the offspring transgenic plants not containing the external source T-DNA sequence can be obtained by directly screening the Basta resistance and performing phenotype combination.

The invention discloses an animal sex control method based on Rbmy gene editing and application. The method controls animal sex by using CRISPR/Cas9 system to specifically cut Rbmy gene on Y chromosome. Rbmy gene encodes a germ cell specific nucleoprotein, this protein is an important factor related to spermatogenesis, and by cutting Rbmy gene, it is possible to deactivate Rbmy gene, thus Y sperm or fertilized ovum with Y sperm is deactivated and cannot develop into a normal embryo, birth ratio of female animals is finally increased and sex control is achieved. Therefore, sex ratio of animal offspring can be controlled through the method so that the ratio of female animals with better economic value is increased and production efficiency and economic effect are greatly improved.

One embodiment of the invention proposes an evolutionary optimization method. In a first step, an initial population of individuals is set up and an original fitness function is applied. Then the offspring individuals having a high evaluated quality value as parents are selected. In a third step, the parents are reproduced to create a plurality of offspring individuals. The quality of the offspring individuals is evaluated by means of a fitness function, wherein selectively the original or an approximate fitness function is used. Finally, the method goes back to the selection step until a termination condition is met. According to an embodiment, the step of evaluating the quality of the offspring individuals consists in grouping all λ offspring individuals in clusters, selecting for each cluster one or a plurality of offspring individuals, resulting in altogether ξ selected offspring individuals, evaluating the ξ selected offspring individuals by means of the original fitness function, and evaluating the remaining λ-ξ offspring individuals by means of the approximate fitness function.

The invention relates to methods of altering gene expression by homologous recombination in a multipotent adult progenitor cell (MAPC). In particular, methods of producing a recombinant MAPC, of correcting a genetic defect in a mammal, of providing a functional and/or therapeutic protein to a mammal, and of transforming a MAPC are provided. MAPCs containing an erogenous DNA as well as recombinant MAPCs and their differentiated progeny are also provided.

The invention discloses a staged desalinization culturing method for penaeus vannamei boone. The method comprises the steps such as selection of penaeus vannamei boone offspring seeds, a staged offspring seed desalting process, treatment on quality of offspring seed desalination water, treatment on quality of water in a culturing process, routine culture and management and the like. By utilizing the staged desalinization culturing method for penaeus vannamei boone provided by the invention, the problem that penaeus vannamei boone dies due to osmotic pressure change caused by salinity change is overcome, and the desalinization culturing of the penaeus vannamei boone is achieved, thus the method provided by the invention has important practical application value.

A method for culturing the minnow of roughskin sculpin includes such steps as capturing the parent fish in near sea in early spring, temporarily culturing in indoor pool, using flowing water to stimulate the parents for estrus, natural mating, ovipositing, collecting fertilized ova, natural incubating in natural seawater in pool, regulating illuminance for correcting the strong phototaxis, applying nutritive forage, improving water quality, and post culturing.