170 results about "Growth Agents" patented technology

A method of propagating mammalian endodermally derived progenitors such as hepatic progenitors, their progeny, or mixtures thereof is developed which includes culturing mammalian progenitors, their progeny, or mixtures thereof on a layer of embryonic mammalian feeder cells in a culture medium. The culture medium can be supplemented with one or more hormones and other growth agents. These hormones and other growth agents can include insulin, dexamethasone, transferrin, nicotinamide, serum albumin, β-mercaptoethanol, free fatty acid, glutamine, CuSO4, and H2SeO3. The culture medium can also include antibiotics. Importantly, the culture medium does not include serum.The invention includes means of inducing the differentiation of the progenitors to their adult fates such as the differentiation of hepatic progenitor cells to hepatocytes or biliary cells by adding, or excluding epidermal growth factor, respectively.The method of producing mammalian progenitors is useful in that the progenitors can be used subsequently in one or more of the following processes: identification of growth and differentiation factors, toxicological studies, drug development, antimicrobial studies, or the preparation of an extracorporeal organ such as a bioartificial liver.

Methods and systems for providing an improved apparatus and packaging system to more expeditiously hydrate or reconstitute medical grafts and to effectively and uniformly seed the medical grafts with biological components and cells. The systems generally comprise a container comprising entry port, at least one substrate cavity, and top, side and bottom walls defining an inner surface. The entry port is configured to receive the biological solution. The cavity is in communication with the entry port and includes the porous substrate maintained under negative pressure. The container volume is substantially the same as a volume of the porous substrate. The side and bottom walls are configured to promote a laminar flow of the biological solution received through the entry port.

The invention provides kitasamycin microcapsule and a preparation method and application thereof, which pertains to a feed drug additive field, in particular relates to novel kitasamycin preparation and a preparation technique thereof, and the application thereof in the preparation of livestock and poultry antibiotic growth promoter. The kitasamycin microcapsule of the invention comprises the kitasamycin and medical auxiliary materials; the weight of the medical auxiliary materials is 2-16 times of that of the kitasamycin material; the microcapsule is prepared through mixing the kitasamycin with heated and melted medical auxiliary materials and spray cooling. The kitasamycin microcapsule eliminates the bitterness of kitasamycin, intensifies the delayed release function of kitasamycin, reduces the stimulation of the kitasamycin to the stomach and the damage and decomposition degree thereof in the stomach, and enhances the effective intestines concentration and the functioning time of kitasamycin; the method for preparing the kitasamycin microcapsule is a wholly close manufacturing process and a wholly close material conveying system, thus decreasing flying of dust and material loss with has high yield of the products.