The invention discloses a mixed feed for promoting the cream growth of females of portunus trituberculatus. The mixed feed is prepared from the following raw material in parts by weight: 20-25 parts of fish meal, 5-10 parts of shrimp shell meal, 5-8 parts of bean meal, 3-5 parts of peanut meal, 3-5 parts of beer yeast, 10-15 parts of high-gluten flour, 5-10 parts of corn flour, 1-3 parts of granulesten, 3-5 parts of fish oil, 0.1-0.3 part of choline chloride, 1-3 parts of monocalcium phosphate, 0.01-0.03 part of butyl hydroxy anisd, 0.01-0.03 part of dibutyl hydroxy toluene, 3-5 parts of Gu Yuan powder, 5-10 parts of rosemary powder, 1-3 parts of a cream growth agent, 1-3 parts of a complex phagostimulant, 0.3-0.5 part of complex vitamin additives and 0.1-0.3 part of complex mineral additives. By utilizing the mixed feed, the growth of the ootheca of the females can be promoted, the content of crab cream in a crab body can be promoted, and the color of the crab cream can be also improved so that the crab cream is red, bright and glossy. The invention also provides a preparation method of the mixed feed for promoting the cream growth of the females of the portunus trituberculatus. The preparation method is simple and suitable for industrial production. The nutrient loss of the mixed feed is low in the preparation process, the forming performance is good, and particles are uniform.