34 results about "Rockfish" patented technology

The invention relates to a method of industrial and artificial breeding of Epinephelus, which is applicable to the breeding of Epinephelus malabaricus and E.coioides. The method comprises the following steps: (1) treating a nursery pond and water; (2) preparing before breeding; (3) putting larval fishes of Epinephelus or germ cells; (4) controlling the quality of the breeding water body; and (5) feeding baits. The method is applied to conduct the industrial and artificial breeding, so that the water quality of the breeding is stable, the output for the unit water body breeding is high, the bred sizes of advanced fries are uniform, no antibacterial drugs are used, no special breeding is conducted to unicellular alga, no bottom-attaching happens in the process of breeding, and the operation is simple.

An industrial breeding method for banded grouper includes such steps as intensified culture of parent grouper, oviposition, incubation, culturing fry and culturing young banded grouper. Its advantages are strong young banded grouper, and high operatability.

The present invention is one kind of green compounded and puffed feed for rock fish. The green compounded and puffed feed is prepared with steamed fish meal 35-50%, soybean residue 15-20%, pork bone powder 5-12%, beer yeast 3-5%, cuttle fish paste 2-6%, fish oil 1.0-2%, vegetable oil 1-2%, macaroni flour 10-20%, shrimp shell powder 3-6%, lecithin 1-2%, vitamins 0.3-0.5%, compounded mineral 0.5-1% and choline chloride 0.3-0.5%, and through mixing, crushing, extruding to pelletize, and stoving. The green compounded and puffed feed has simple compounding process and balanced nutrients, can meet the requirement of the growth of rock fish, and results in less water contamination.

A rockfishes pond culture method is disclosed, wherein young fish of rockfishes is released in a sea pond with water depth more than 80 cm and water temperature 13-38 DEG C, released density is 750-850 fish 667 m2, diet is feed at least twice a day in the release process, quantity of diet feeding is: 3-4% fish weight in the early breeding stage, 4-5% in the middle stage and 5-7% in the late stage; specification of the released fish is 6-8 per kg, the release pond is sand bottom, and water in the pond is more than 1.5 in depth, and provided with a separate inlet / discharge channel. The bottom of the pond is laid with a plurality of random rubbles or perforate junked tires or tile tunnels. The invention is characterized by simple breeding method, low breeding cost, high success ratio of the cultivated fish, healthy in water aquatic products with high quality and nuisance free and the like.

A microgranular bait for the fry of Epinephelus is prepared from crude protein, crude fat, phosphatide, ash, n-3HUFA, DHA / EPA, DHA, and EPA according to a certain proportion through enzymolyzing by proteinase, further adding antioxidizing agent, filtering, and spray drying. Its advantages are rich nutrients, and low settling speed.

The present invention is rockfish viral nervous virus gene diagnosing kit and its usage. The kit and its usage is designed based on two pairs of primers for the conservation sequence of rockfish viral nervous virus gene. By means of PCR technology, the present invention detects qualitatively the specific DNA nucleic acid segment of tiger frog virus fast, simply, specifically and sensitively. The present invention may be used in the tracing detection of different stages of rockfish cultivation and environment monitoring.

The present invention relates to one pair of PCR primers named as G-dloop, and provides one pair of amplification primers capable of amplifying the high variation zone of rockfish mitochondrial genome in high efficiency and its design method. The amplification primers consist of two single strand oligonucleotides. Through logging-on Genebank to search vertebrate mitochondrion DNA cytochrome b gene and the high conservation area of 16S rRNA gene sequence and homologous comparison, one pair of amplification primers is obtained. Through long PCR amplification, the target segments of 32 varieties of rockfish are obtained and sequenced. The obtained sequences are compared by means of using homologous comparison software Clustal X 1.83 to fine the conservation sequences in the cytochrome b5' ends and the 12S rRNA 3' ends of the mitochondrial genomes of the 32 varieties of rockfish, and the said amplification primers are designed based on the degeneracy principle.

The invention relates to an artificial forced mature method to girth rockfishes, characterized in that adding yeast cell wall, mixture with abio salinous, phytic acid enzyme and methyl testis ketone, etc, into bait to force male parent fish to mature in web box on sea and enhanced feed in doors' pool, then by injecting DOM and ecology stimulus forces parent fish gonad to mature and oviposit naturally. This invention can achieve artificial control on parent fish sex of girth rockfishes with at least 70% triumph rate on sex adjustment. Meanwhile young functional male fish gained can be also used to production, which makes more than 50% parent fish come to sex mature and oviposit naturally and obtains mass high class gamete, thereby improve utilization rate of parent fish and productive capacity.

A nutritive-enriched feed for the parent rockfish is prepared from fresh small fish and / or squid, and nutrients including fish oil, VE, VC lecithin, fish powder, alpha-starch or dextrin, egg lecithin and astaxanthin through proportionally mixing said nutrients with water, and inserting the mixture in the cavity of small fish or squid.

The present invention discloses one kind of rockfish insulin-like growth factor II gene. The gene is gene segment of rockfish insulin-like growth factor II gene whole length cDNA obtained with rockfish liver total RNA as template and through RT-PCR and RACE process. The present inventon also constitutes carrier and recombinant strain containing the gene. Utilizing the present invention can obtain recombinant rockfish insulin-like growth factor II protein with stable source and low cost and further prepares fry growth promoter or additive suitable for sea water fishes.

A slow-releasing oxytocic for artificially inducing the maturation of epinephelus' sex gland and the ovulation or ejaculation by embedding it in body of epinephelus is prepared from luliberin like, 17 alpha-hydroxyprogesterone, cholesterin, cellulose and plant oil through proportional mixing and moulding.

The recombinant rockfish adenylate cyclase qctivated polypeptide gene is obtained with rockfish brain tissue general RNA as template and through RT-PCR and RACE process and is the gene segment with whole-length cDNA sequence of rockfish adenylate cyclase qctivated polypeptide gene. The present invention also constructs carrier containing the gene and recombinant engineering strain. The present invention also relates to rockfish adenylate cyclase qctivated polypeptide recombined protein encoded with the gene and expressed with the recombined engineering strain, and the production process of the recombined protein. The present invention makes it possible to obtain rockfish adenylate cyclase qctivated polypeptide recombined protein with stable soruce and low cost for preparing growth and propagation regulator suitable for marine fishes.

The invention pertains a kind of phagostimulant to rockfishes and other fishes, and its produce way. Materials by weight: animal protease 70-85%, nucleotide 2-10%, sulphocompound 0.4-3%, complex aminoacid 5-15%, betaine 2-20%, pigment 0.5-5%, oxidation preventive 0.1-0.5%. Production way: add animal protease after enzymolysis in oxidation preventive of 0.5-3% of fresh animal protease by weight; add by rate nucleotide, sulphocompound, complex aminoacid, betaine, pigment, oxidation preventive, mix and dry them, then the phagostimulant is made over. This phagostimulant can absorb sober of water animal effectually, promote growing, increase the ability of defeat disease, and increase the rate of living.

The present invention relates to one pair of PCR primers named as G-dloop, and provides one pair of amplification primers capable of amplifying the high variation zone of rockfish mitochondrial genome in high efficiency and its design method. The amplification primers consist of two single strand oligonucleotides. Through logging-on Genebank to search vertebrate mitochondrion DNA cytochrome b gene and the high conservation area of 16S rRNA gene sequence and homologous comparison, one pair of amplification primers is obtained. Through long PCR amplification, the target segments of 32 varietiesof rockfish are obtained and sequenced. The obtained sequences are compared by means of using homologous comparison software Clustal X 1.83 to fine the conservation sequences in the cytochrome b5' ends and the 12S rRNA 3' ends of the mitochondrial genomes of the 32 varieties of rockfish, and the said amplification primers are designed based on the degeneracy principle.

The present invention discloses one kind of rockfish sex reversal relative gene sequence, vector and recombinant strain containing the gene and their use. The gene has cDNA of 608 bp length, one open reading frame of 252 bp (54-305), 83 coded amino acids, 5' non-coding region of 53 bp length and starting at one ANNATG motif included inside vertebrate initiator codon, and 3' non-coding region of 303 bp length and with polypeptide tailing signal (AAUAAA) and polypeptide tail. The vector and recombinant strain containing the gene and protoyon expressed fusion protein are used in propagation regulation, sex gland differentiation and sex conversion of rockfish and the feed additive preparation.

The medicine has mixed hormone comprising methyl testosterone, testosterone, propiotestosterone and thyroxine, and the mixture is added with algal polysaccharides or alpha-starch and prepared into mediicne stip suitable for embedded into fish body. The medicine of the present invention is embedded into female rockfish's body to induce sexual reversion. Practical test shows that using the mixed external hormone of the medicine can shorten female rockfish's sexual reversion period to 80 days with stable and reliable effect and no sexual restoring.

The invention discloses three kinds of rockfish somatostatin genes, which are a gene fragment with rockfish somatostatin gene cDNA sequence, obtained by using rockfish hypothalamic total RNA as a template and by the RT-PCR and Gene RACER methods; the three genes has an important application to the rockfish growth.

An industrial breeding method for banded grouper includes such steps as intensified culture of parent grouper, oviposition, incubation, culturing fry and culturing young banded grouper. Its advantages are strong young banded grouper, and high operatability.

The invention discloses a slow-release oxytocic agent for artificially inducing grouper gonad development and maturation, ovulation and ejaculation. 10%, 75-95% cholesterol, 1-19% cellulose; 1-3% vegetable oil is added to the formula components, mixed evenly, and cylindrical pills are made with a special mold. The invention is used to bury the grouper in the body to induce the maturation of the gonads to achieve ovulation and sperm discharge, so as to meet the needs of the grouper artificial seedling industrialization and large-scale breeding for seedlings.

A gene sequence of an embryonic development regulatory factor in the scapus of Epinephelus has 514 bp for cDNA length, 252 bp for open reading frame, 83 coding amino acids, 53 bp for 5' non-coding region, and 209 bp for 3' non-coding region. It plays the important role in early development stage, and can be used in artificial breeding.

The rockfish feed features that the rockfish feed contains saccharomycete strain modified in plasma environment. The modified saccharomycete, which may be edible saccharomycete for brewing wine, has activity strengthened in geometric series, and adding the modified saccharomycete into rockfish feed can strengthen the disease resistance of rockfish greatly and reduce or eliminate effect of rockfish viruses on rockfish.

The invention relates to a method of industrial and artificial breeding of Epinephelus, which is applicable to the breeding of Epinephelus malabaricus and E.coioides. The method comprises the following steps: (1) treating a nursery pond and water; (2) preparing before breeding; (3) putting larval fishes of Epinephelus or germ cells; (4) controlling the quality of the breeding water body; and (5) feeding baits. The method is applied to conduct the industrial and artificial breeding, so that the water quality of the breeding is stable, the output for the unit water body breeding is high, the bred sizes of advanced fries are uniform, no antibacterial drugs are used, no special breeding is conducted to unicellular alga, no bottom-attaching happens in the process of breeding, and the operationis simple.

The invention relates to a breed method on anti-vibrio for girth rockfishes, characterized in that dipping in anti-vibrio bacterin twice on the day of 5-10 and 10-16 after fry's birth, whose molar concentration follows 1.0-2.0mg / L and 0.5-1.2mg / L, the time interval to dip in should be 5-8 days and the rotifer and brine shrimp in fry bait should be cultivated with enrichment in the water with chlorella of 30, 0000-50, 0000 cell number per milliliter and unsaturated fatty acid of W3 with molar concentration 0.03mg-0.06mg / L, whom should be fed to young fry after cultivating with enrichment 6-8h. The survival rate of the girth rockfish fry fed on this method, that 1-3 days age is 76.4%-80.2%, 5-10 days age is 68.1%-72.3%, 10-15 days age is 50.2%-53.6%, 15-25days age is 42.7%-47.3%, and the anti-vibrio rate can reach 65.2%-72.6%.

The invention relates to a breed method on anti-vibrio for girth rockfishes, characterized in that dipping in anti-vibrio bacterin twice on the day of 5-10 and 10-16 after fry's birth, whose molar concentration follows 1.0-2.0mg / L and 0.5-1.2mg / L, the time interval to dip in should be 5-8 days and the rotifer and brine shrimp in fry bait should be cultivated with enrichment in the water with chlorella of 30, 0000-50, 0000 cell number per milliliter and unsaturated fatty acid of W3 with molar concentration 0.03mg-0.06mg / L, whom should be fed to young fry after cultivating with enrichment 6-8h. The survival rate of the girth rockfish fry fed on this method, that 1-3 days age is 76.4%-80.2%, 5-10 days age is 68.1%-72.3%, 10-15 days age is 50.2%-53.6%, 15-25days age is 42.7%-47.3%, and the anti-vibrio rate can reach 65.2%-72.6%.

The invention provides explosion hook bait for rockfishes. The explosion hook bait consists of the following raw materials: river snail powder, shrimp powder, corn protein powder, peanut protein powder, loach powder, pork liver powder, bone powder, high-efficiency phagostimulant powder, dispersing agent powder for fishes, edible swelling agent powder, high-efficiency bonding agent powder, wire-drawing powder, in-water incense powder, clamworm powder and soybean protein powder. The preparation method comprises the following steps of: mixing all raw materials in a ratio, feeding the mixture in a stirrer for stirring for 200 minutes, putting the stirred mixture in a drying machine, wherein the temperature of the drying machine is controlled to between 80 and 85 DEG C, the drying time is 2 hours, and the dryness of the mixture is controlled to 75 to 85 percent; taking the mixture out for cooling, feeding the cooled mixture in the stirrer for stirring for 200 minutes again, feeding the mixture into a bulking machine for bulking, wherein the temperature of the bulking machine is controlled to be between 85 and 95 DEG C, the bulking time is 100 minutes, and the dryness of the mixture is controlled to be 85 to 95 percent; and taking the mixture out for cooling, and packaging the cooled mixture to obtain the finished product.

The present invention is rockfish viral nervous virus gene diagnosing kit and its usage. The kit and its usage is designed based on two pairs of primers for the conservation sequence of rockfish viral nervous virus gene. By means of PCR technology, the present invention detects qualitatively the specific DNA nucleic acid segment of tiger frog virus fast, simply, specifically and sensitively. The present invention may be used in the tracing detection of different stages of rockfish cultivation and environment monitoring.

The invention discloses a manufacturing method of spicy dried fish. The method comprises the following steps that S1, cave rockfish is cleaned; S2, water is poured into a pot; Jiangyong alpinia coriandriodora is fragmented; rice vinegar, dry chilli, table salt and chipped ginger are sequentially put into the water pot; S3, the water is boiled; S4, the cave rockfish is put into a strainer; the caverockfish is placed in the water pot to be boiled by the strainer; S5, the boiled rockfish is fished from the pot for use; S6, a field is selected for grass paving; a plurality of bamboo sieves are placed on the grass surface; the cave rockfish fished from the pot is dispersed and arranged on each bamboo sieve for drying ; S7, the dried rockfish is subjected to smoke drying; S8, the spicy dried fish after the smoke drying is packaged. The manufacturing method has the advantages that the manufacturing is simple and convenient; the fishy smell can be removed; the unique mouthfeel can be formed;the food safety can be improved.

A nutritive-enriched feed for the parent rockfish is prepared from fresh small fish and / or squid, and nutrients including fish oil, VE, VC lecithin, fish powder, alpha-starch or dextrin, egg lecithin and astaxanthin through proportionally mixing said nutrients with water, and inserting the mixture in the cavity of small fish or squid.