55 results about "Epinephelus bruneus" patented technology

The invention relates to a method of industrial and artificial breeding of Epinephelus, which is applicable to the breeding of Epinephelus malabaricus and E.coioides. The method comprises the following steps: (1) treating a nursery pond and water; (2) preparing before breeding; (3) putting larval fishes of Epinephelus or germ cells; (4) controlling the quality of the breeding water body; and (5) feeding baits. The method is applied to conduct the industrial and artificial breeding, so that the water quality of the breeding is stable, the output for the unit water body breeding is high, the bred sizes of advanced fries are uniform, no antibacterial drugs are used, no special breeding is conducted to unicellular alga, no bottom-attaching happens in the process of breeding, and the operation is simple.

An industrial breeding method for banded grouper includes such steps as intensified culture of parent grouper, oviposition, incubation, culturing fry and culturing young banded grouper. Its advantages are strong young banded grouper, and high operatability.

The invention discloses a method for culturing Epinephelus coioides at a low salt degree, and relates to a culturing method for aquatic livestock, in particular to a method for culturing the Epinephelus coioides. The method comprises the following steps of: desalting sea water for culturing healthy Epinephelus coioides at a rate of decreasing 0.1 to 0.2 percent of salt degree per 24 hours; and when the salt degree is desalted to 0.8 percent, putting the Epinephelus coioides into a pond in which the salt degree of the water body is 0.8 percent for culturing, wherein the salt degree of the sea water is greater than 2.0 percent. By manually desalting and domesticating gradually, a pond culturing mode of the Epinephelus coioides is optimized so that the survival rate reaches over 70 percent. The yield of the Epinephelus coioides in each pot is over 150 kilograms; and the yield of the Epinephelus coioides of each mu of the pond is 500 kilograms.

The invention relates to a recombinant nerve putrescence virus protein vaccine, the nucleic acid sequence coding the recombinant protein, and the use of the recombinant protein in preparing fish use vaccine product for nerve putrescence viruses resistance. Wherein the cDNA of the capsid nucleocapsid for the grouper nerve putrescence viruses is utilized to obtain the polypeptide recombinant proteins through DNA recombination process and pronucleus expression method.

A microsatellite multiple fluorescent PCR method for E. coioides paternity testing is disclosed. The method includes (1) a step of extracting individual DNAs of E. coioides; (2) a step of screening E. coioides polymorphic microsatellite primers; (3) a step of optimizing E. coioides eightfold PCR conditions and performing amplification; and (4) a step of performing paternity testing. The method combines microsatellite marking and a multiple fluorescent PCR technique, eight polymorphic microsatellite loci are screened and an eightfold fluorescent PCR system is built. The eightfold fluorescent PCR system is verified by utilizing the E. coioides family. The method is simple and rapid to operate and low in cost, can be popularized and applied in E. coioides germplasm identification, family management and improved-variety selection, and provides a novel technical means for assessment of enhancement and releasing effects.

The invention discloses a method for cyclically cultivating Epinephelus coioides with artificial seawater indoors, which comprises the following steps of preparation of artificial seawater, preparation of cultivation water, selection of fry, regulation and control of water quality, selection of feed, daily sewage suction and water change, and cleaness maintenance of the bottom and walls of a pond. The method is efficient and environment-friendly; Epinephelus coioides obtained according to the method can be high in quality and yield, and healthy, in addition the cultivating survival rate is high, and the feed coefficient is low.

The invention discloses an SNP (single nucleotide polymorphism) marker and application thereof. The SNP marker is the 381st base T or C from the 5' terminal of a nucleotide sequence as shown in the SEQ ID NO:1. The SNP marker is closely related to the weight trait of epinephelus coioides, and can be effectively used for molecular mark assisted breeding of epinephelus coioides.

The present invention discloses one kind of Epinephelus coioide culturing method and aims at provides Epinephelus coioide culturing method with high efficiency, no environmental pollution and adult fish eating safety. The method includes constructing indoor culture pond and biological filtering pond, throwing Epinephelus coioide fry of 50 g weight into the culture pond and raising with bait while controlling the conditions essential for Epinephelus coioide to grow. The indoor raising method has no escape of Epinephelus coioide, fast growth and high raising efficiency. The communicated indoor culture pond and biological filtering pond can ensure high water quality, less fish disease, no environmental pollution and adult fish eating safety. The technology of the present invention is applicable in culture of other fish.

The invention relates to a preparation method of an inactivated vaccine for the iridovirus of a grouper, comprising the following steps of: inoculating the iridovirus to embryonic cells of the grouper in a logarithmic phase by taking an embryonic fine system of the grouper as an amplification system of the iridovirus; repeatedly freezing and thawing and centrifugalizing after complete lesion, and inactivating an obtained iridovirus solution at 4 DEG C for 16 hours by using beta-propiolactone with the final concentration of 1:500 so as to obtain the inactivated iridovirus vaccine. The inactivated iridovirus vaccine is applied to a juvenile immunized Malabar grouper, and an iridovirus counteracting result shows that relative protection ratio is more than 90 percent after 15 days. The preparation method has the advantages of easiness and convenience for operation, simple equipment requirement and good repeatability and keeps good immunogenicity of the iridovirus under the precondition of efficiently inactivating the iridovirus, thereby having good immune protection effect; in addition, the invention can be used for the preventive immune of the grouper, thereby enhancing the survival rate and the culturing efficiency of cultured groupers.

The invention discloses an SNP marker relevant to ammonia endurance capacity of epinephelus coioides and the use of the SNP marker. The nucleotide sequence of the SNP marker is as shown in SEQ ID NO.1, and the 501st basic group from 5' end in the sequence is G or T. The SNP marker is used for molecular marker auxiliary breeding of the epinephelus coioides. The invention further provides a primer and reagent kit for detecting the SNP marker relevant to the ammonia endurance capacity of epinephelus coioides, and the use of the primer and the reagent kit to breeding of the epinephelus coioides. The invention also provides a method for detecting the ammonia endurance capacity of the epinephelus coioides through the SNP marker, the primer and the reagent kit. Through the adoption of the SNP marker disclosed by the invention, the ammonia endurance capacity of the epinephelus coioides can be detected quickly, efficiently and accurately, and further the SNP marker can be effectively used for molecular marker auxiliary breeding of the epinephelus coioides, so that short-time low-cost high-accuracy breeding of the epinephelus coioides of good varieties can be early realized in an auxiliary manner.

The invention discloses an epinephelus coioicles ecological feed formula and a preparation method. The epinephelus coioicles ecological feed is prepared from fish meal, tenebrio molitor powder, nereis powder, earthworm powder, silkworm chrysalis meal, shrimp head meal, blood meal, squid visceral powder, beer yeast, bean pulp, soybean phospholipid, alpha-starch, flour, alfalfa meal, moringa powder, macroalgae mixed powder, fish oil, shell mixed powder, dry ginger powder, choline chloride, composite multiple vitamin powder and a phytase preparation. The raw materials are weighed according the proportions, cleaned, dried, smashed and put into a vessel to be stirred to be uniform, granulation is conducted through fluidized bed spray, feed particles are cooled, subpackaged and sealed, and the target product is obtained. According to the epinephelus coioicles ecological feed, feeding is convenient, storing is convenient, the feed quality is high, the nutritional requirement of epinephelus coioicles is met, preparation is dry and thorough, long-term storing is convenient, the feed is not prone to deterioration, active substances in tenebrio molitor, nereides, silkworm chrysalises and fresh ginger can not be subjected to inactivation, the utilization rate of the epinephelus coioicles to the feed is increased, and the growth performance of the epinephelus coioicles is improved.

The invention belongs to the field of aquaculture and relates to a method for preventing and controlling prawn communicable diseases by Epinephelus coioids. The method comprises the steps of: releasing young prawns in high-salinity seawater, wherein the releasing amount is 80,000-10,0000 fish per mu (mu refers to a unit of area); when the prawn is cultivated to 0.3-0.7 gram, releasing the Epinephelus coioids into the seawater, wherein the weight of each Epinephelus coioids is 0.10-0.15 kg, and the releasing amount of the Epinephelus coioids is 15-20 fish per mu. Large amount of experiments prove that: the Epinephelus coioids about 0.10-0.15 kg in weight can eat ill and dead prawns in a culture pond, the probability of eating healthy prawns is very little due to the habit of the healthy prawns. According to the characteristic, the problem that the survival rate of the bred prawns is low because the healthy prawns are eaten is solved, and simultaneously, the prawn communicable diseases are effectively prevented and controlled.

The invention discloses a cloning vector containing a mature peptide gene GTHalpha or FSHbeta of an epinephelus coioids gonadotropin hormone, an eukaryotic expression vector, a recombinant strain, a recombinant protein and application of the recombinant protein to the preparation of a fish oxytocic. Epinephelus coioids belongs to a rare marine fish and has high economic value. The recombinant protein for recombining the epinephelus coioids gonadotropin hormone I is produced by adopting a gene-engineering technology and can be used for preparing the fish oxytocic with low preparation cost and remarkable effect.

The invention discloses an SNP (single nucleotide polymorphism) marker and application thereof. The SNP marker is the 251st base T or C from the 5' terminal of a nucleotide sequence as shown in the SEQ ID NO:1. The SNP marker is closely related to the overall length trait of epinephelus coioides, and can be effectively used for molecular mark assisted breeding of epinephelus coioides.

The invention discloses a compound feed with low content of fish meal for epinephelus coioides, and relates to the technical field of fish feeds. The compound feed consists of the following components in parts by weight: 12 to 18 parts of fish meal, 28 to 40 parts of casein, 7 to 10 parts of gelatin, 2 to 4 parts of fish oil, 2 to 4 parts of soybean oil, 1 to 3 parts of granulesten, 8 to 35 parts of corn starch, 1 to 2 parts of monocalcium phosphate, 0.2 to 0.6 part of compound vitamin premix, 0.2 to 0.6 part of compound trace element premix, 0.3 to 0.8 part of taurine, 1 to 2 parts of sodium alginate, 0.1 to 0.3 part of choline chloride, 2 to 12 parts of zeolite powder, and 2 to 12 parts of microcrystalline cellulose powder; the raw materials are crushed, screened, mixed and granulated, so as to prepare the compound feed for the epinephelus coioides. The compound feed has the advantages that the obtaining of raw materials is easy, the nutrients are balanced, the processing is simple and convenient, the usage ratio and amount of fish meal in the feed for the epinephelus coioide are reduced, the cost is low, and the compound feed is suitable for industrialized popularization.

The invention discloses an artificial fry breeding method for epinephelus coioides. The breeding process includes the steps of roe laying and hatching, larva breeding, fry grading, and juvenile fish breeding. The method has the advantages that according to the development characteristics of feeding organs of epinephelus coioides larvae, a matched bait line for feeding oyster fertilized eggs, rotifers and artemia is formulated, the method is scientific and reasonable, and the growth speed of the larvae is high; the natural growth environment of the epinephelus coioides is simulated, the environment suitable for hatching of fertilized eggs of the epinephelus coioides and growth of the larvae is created, and the survival rate of the artificially bred epinephelus coioides is increased; in the larvae breeding stage, a chlorella solution containing active polypeptides is injected into pond water, so that the leukopoiesis capacity of the larvae is improved, the non-specific immunity of the larvae is enhanced, and the anti-stress capability of the larvae is improved.

The invention provides a preparation method and application of a polyclonal antibody against CCR12 of Epinephelus coioides. Currently, three XCR1-like chemokine receptors are discovered in fish, i.e., XCR1, XCR1L and CCR12, wherein XCR1L and CCR12 are only discovered in the genomes of fish, but little research is conducted on the functions of XCR1L and CCR12; and so far, in-vitro expression of CCR12 from fish has not been reported, no one has prepared a polyclonal antibody against CCR12 of Epinephelus coioides, and application of the polyclonal antibody against CCR12 has not been reported, either. To further reveal the migration or propagation of Epinephelus coioides CCR12 positive cells in tissue with parasitic infections and to identify the types of the CCR12 positive cells, a recombinant protein of the N-terminal region and three extracellular regions the CCR12 is expressed via a prokaryotic system and purified, and a rabbit anti-CCR12 recombinant protein polyclonal antibody is eventually prepared for the first time; and the antibody can specific recognize the CCR12 protein of Epinephelus coioides and mark cells in blood, so a foundation is laid for further marking of the CCR12 positive cells and research on parasitic infection resistance of the CCR12 positive cells.

The invention discloses a method for preventing and controlling shrimp communicable diseases through twice epinephelus coioids release. In the method, epinephelus coioids of two different size specifications are released in a prawn culture region twice, thus the prevention and control of the peneldshrimp communicable diseases can be realized, and the survival rate of prawns can be improved. By utilizing the method disclosed by the invention, shrimp diseases can be prevented from being spread and popularized among healthy shrimp clusters, the work load of artificially removing sick shrimps and died shrimps is reduced, the removing efficiency and the culture success ratio are improved, and greater economic benefits are brought to culturists.

The present invention discloses a taurine compound preparation for groupers and an application thereof. The taurine compound preparation is composed of the following raw materials in parts by weight: 30-70 parts of taurine, 10-40 parts of alanyl-glutamine, 2-6 parts of vitamin C polyphosphate, 1-5 parts of vitamin E and 4-19 parts of corn starch. The taurine compound preparation for the groupers has the advantages of promoting the growth and feed conversion efficiency of the groupers, reducing the ratio of liver weight and body weight, reducing the deposition of body fat and liver fat of the groupers, can significantly improve the activity and total antioxidant capacity of superoxide dismutase, catalase, serum antioxidant enzymes and glutathione peroxidase in liver and plasma of saddletail groupers, and at the same time significantly reduces the malondialdehyde content.

The invention relates to an epinephelus coioides interferon IFNgamma2 gene and a protein encoded by the sameas well as application of the protein in preparing a novel immunopotentiator. According to the invention, an open reading frame of the IFNgamma2 gene is cloned from epinephelus coioides head-kidney total RNA by specific primers SEQ ID NO:4 and SEQ ID NO:5 by means of a method of a primer amplification gene segment and RACE overall length amplification. The nucleotide sequence is shown as SEQ ID NO:2. The amino acid sequence of encoded protein is shown as SEQ ID NO:1. An initial test shows that the epinephelus coioides interferon IFNgamma2 protein can induce relevant immunogene expression and can be developed to natural fish immunopotentiator or immunologic adjuvant.

The invention discloses an epinephelus coioides feed additive and an application method thereof. The epinephelus coioides feed additive is composed of a photosynthetic bacteria liquid, a bacillus pumilus powder and a lactobacillus acidophilus liquid. The bacillus pumilus powder is firstly mixed with the lactobacillus acidophilus liquid, and then the mixed bacteria is mixed with the photosynthetic bacteria liquid. The mass percentage content scope of the three compositions comprise that the photosynthetic bacteria liquid accounts for 18-22%, the bacillus pumilus powder accounts for 1-3%, and the lactobacillus acidophilus liquid accounts for 75-81%. Based on 1 Kg of base feed, 10 g-50 g of the additive is added, and the additive is uniformly mixed with the base feed, and then the mixture is wrapped by emulsified fish oil. The epinephelus coioides feed additive is suitable for healthy culture of epinephelus coioides, is capable of obviously improving the activity of SOD, AKP and lysozyme in fish body, thereby effectively promoting growth of epinephelus coioides.

The invention belongs to the technical field of genetic engineering, and in particular discloses an epinephelus coioides insulin gene, coded protein and an application thereof. The nucleotide sequenceof the insulin protein is shown as SEQ ID NO:1, and the amino acid sequence of the insulin protein coded by the gene is shown as SEQ ID NO.2. The epinephelus coioides insulin gene provided by the invention is obtained for the first time through cloning, and the gene is applied to construction of an expression vector and a recombinant escherichia coli strain; the recombinant epinephelus coioides insulin protein, which is high in expression, is obtained on the basis of the recombinant escherichia coli strain in vitro; and the protein, which can promote glycolipid utilization and growth of suchmarine fishes as epinephelus coioides and the like, can be used for preparing growth promoters or bait additives applicable to the marine fishes; therefore, the protein has a relatively broad application prospect.

The invention discloses a culturing method for epinephelus coioides. The culturing method comprises steps of 1) preparation before releasing, 2) releasing seedlings, 3) changing food and domestication, and 4) daily management. The beneficial effects are that according to physiological characteristics, a culturing environment condition is controlled; according to growth and development conditions of an Epinephelus coioides digestion system, food is changed slowly and the Epinephelus coioides are domesticated; the Epinephelus coioides have fast growth speed and vigorous vitality; ammonia nitrogen organic matters in water can work as nitrogen source and carbon sources by microorganism agents; the Epinephelus coioides can be biochemically dissolved; beneficial bacteria flora largely propagate to form advanced flora, and poisonous bacteria breeding in water can be prevented; catechol and phenol in water can be removed; flocculation effect can be achieved; and water quality clarity can be improved.

Disclosed is an aromatase inhibitor which is a key enzyme for catalyzing the conversion from androgen to estrogen in animal body, by inhibiting activity of aromatized enzyme, the level of estrogen can be lowered, so as to evoke the animal towards male direction. The invention can be applied to the inducement of sex inversion in advance for Epinephelus coioides with the advantages of easy operation, substantial effects, and no hormones contamination to the environment.

The invention discloses an epinephelus coioides antimicrobial peptide. The antimicrobial peptide is epinephelus coioides CD59 protein, and has an amino acid sequence as shown in SEQ ID NO.1, or protein having an amino acid sequence as shown in SEQ ID NO.1, which is substituted, lost and / or added with one or more amino acids and / or is terminal modified and has the same or higher activity. Experiments prove that the epinephelus coioides antimicrobial peptide can be used for inhibiting the growth activity of vibrio alginolyticus, can be applied to preparation of immune stimulant or feed additivesfor fish, especially groupers, and has a wide application prospect. The CD59 gene sequence of the epinephelus coioides is obtained for the first time, can be used for enriching the gene pool of groupers, can be applied to preparation of recombinant protein, antibacterial and bacteriostatic additive and fish immune simulants, and provides new practice foundation for physiological immune researchesof epinephelus coioides.

The invention discloses a feed containing highly unsaturated fatty acid used for improving the growth performance and meat quality of epinephelus coioides. The feed comprises the following componentsin percentage by weight: 55-65% of protein-source raw material, 6-10% of fat-source raw material, 2-4% of water-soluble dietary fibers, 1-5% of compound vitamins, 1-5% of compound mineral salts, 4-10%of an adhesive, 0.1-1% of choline chloride, 0.1-1% of vitamin C phosphate and the balance of sugar-source raw material, wherein the fat-source raw material is vegetable oil containing DHA and EPA, the total amount of the DHA and the EPA is 1-2% of the feed by weight, and the dosage ratio of the DHA to the EPA is (1-4):1. By adjusting the formula of a feed for epinephelus, the feed of the invention can obviously improve the growth performance and the nutritional value of the epinephelus, and has a good popularization value and application prospect.

The invention relates to an epinephelus coioides interferon IFNgamma1 gene, a protein coded by the gene and an application of the protein in preparing a novel immunopotentiator. According to the invention, an opened reading frame of the IFNgamma1 gene is cloned from head-kidney RNAs (Ribonucleic Acid) of epinephelus coioides through a method of screening a genome database and designing specific primer amplification. The nucleotide sequence of the gene is shown as SEQIDNO:2. The amino acid sequence of the protein coded is shown as SEQIDNO:1. Through initial verification, the epinephelus coioides interferon IFNgamma1 protein can induce associated expression of immunogenes and can be developed to natural immunopotentiators or immunologic adjuvants for fishes.

The present invention relates to Epinephelus coioides SRY box relative gene Sox and its application. The present invention finds the gene Sox from the sex gland of Epinephelus coioides and the gene has important application in sex gland development and sex reversal of Epinephelus coioides.

The invention adopts a method of cryptocaryon irritans locally-infected epinephelus coioides one-sided skin for immunity for the first time, adopts an optimized infection concentration and infection duration time to construct an immune model of the cryptocaryon irritans locally-infected epinephelus coioides one-sided skin for the first time, thoroughly eliminates an adverse effect brought by ''congenital differences'' in the process of local skin mucosal immune response in fish, and provides an ideal immune model for researching a local mucosal immune response law. The construction method forthe immune model mainly comprises the following five steps: (1) collecting live larvae of the cryptocaryon irritans and calculating a concentration; (2) grouping the epinephelus coioides and performing domestication; and (3) performing local skin immunization; (4) measuring infection intensity; and (5) performing treatment on locally-infected fish bodies.

The present invention relates to hybrids of brown grouper and grouper and their production methods, and more particularly to the use of two species of brown grouper and grouper in two families, and hybrids between the male brown grouper and the male grouper grouper and the hybrid of the male brown grouper and the female grouper grouper production method. The present invention can provide hybrids with high growth rate and excellent quality through the hybridization between highly marketed brown grouper and fast-growing and commercially available grouper grouper. The growth of the initial larvae in the hybrids of the present invention is so fast as to solve the problem of biological bait that becomes a major problem when breeding fish species, thereby contributing to the activation of the Korean aquaculture industry.