The invention relates to a one-bacterium multiple-enzyme bacterial strain as well as a screening method and an application thereof. The bacterial strain is bacillus subtilis (Bacillus subtilis 1.1111) and is collected in the China center for type culture collection with the collection number of CCTCC (China center for type culture collection) No: M2011286. The bacillus subtilis (Bacillus subtilis 1.1111) can be used for preparing the bacterial strains of nine enzymes, i.e. xylanase, protease, phytase, pectinase, lipase, sweet dew glucanase, glucoamylase and the like, and the yields of the protease, the sweet dew glucanase, amylase and the glucoamylase are very high. Meanwhile, the bacterial strain is proved to have strong endurance capacity on cholate, artificial gastric juice and artificial intestinal juice by simulating the internal cholate environment, the artificial gastric juice, artificial intestinal juice and the animal test, safety and growth simulation capability are shown to a tested animal, and a foundation is laid for effectively improving the enzyme production capability of the bacterial strain, simultaneously generating the xylanase, the protease, the phytase, the pectinase, the lipase, the sweet dew glucanase and the glucoamylase and realizing one-bacterium multiple-enzyme fermentation in the fermentation process. The mutual synergistic effect among various enzymes generated by the bacterial strain is strong, and the bacterial strain can be used as a feed additive to be applied to agricultural production for livestock, fowls, aquatic livestock and the like.