The invention relates to a spectral ecological agricultural greenhouse energy gathering film and a preparation method thereof, and belongs to the technical field of agricultural plastic films. The agricultural greenhouse energy gathering film comprises a three-layer structure of an inner layer, a middle layer and an outer layer and is mainly prepared from mLLDPE, LLDPE, LDPE, EVA, single anti-aging masterbatch, rheological agent masterbatch and spectral ecological organic masterbatch; the preparation method comprises the steps that all components are mixed uniformly according to the formula amount, then a three-layer film blowing machine is used for film blowing, and the spectral ecological agricultural greenhouse energy gathering film is obtained after extrusion, plastic film forming, blowing, corona treatment, coating, drying and folding. According to the spectral ecological agricultural greenhouse energy gathering film and the preparation method thereof, all disadvantages caused by the fact that a light conversion agent is merely used for changing light in the greenhouse are changed, collineation of the light is changed into scattering and refraction by adding a double peak material and a reddish orange auxiliary, the spectrum peak of the light transmitted into the greenhouse can be adjusted into the red orange light band suitable for plant growth, the light intensity can be kept continuously, no half-life period exists, and the own energy of the light power is fully used.