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Biological agent capable of repairing heavy metal contaminated soil and soil remediation method

A technology for contaminated soil, biological agents, applied in the restoration of contaminated soil, microorganism-based methods, biochemical equipment and methods, etc., can solve problems such as undiscovered biological agents

Inactive Publication Date: 2013-05-22
广州天壤生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] After literature and patent searches, no reports were found on the use of photosynthetic bacteria to prepare biological agents and apply them to the remediation of heavy metal pollution in soil

Method used

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Examples

Experimental program
Comparison scheme
Effect test

preparation example Construction

[0032] The preparation method of the biological agent for remediating heavy metal-contaminated soil, the production process is the process of expanding the cultivation of microorganisms step by step, and the specific cultivation process steps included are as follows: (1) Take the original strains respectively; (2) Cultivate the first-level strains; 3) Secondary strain cultivation; (4) Seed tank cultivation; (5) Fermentation tank cultivation; (6) (Qualified inspection) aseptic filling. Wherein, the first-level strain culture and the second-level strain culture adopt light of 1000-3000LUX, culture temperature 25-30°C, and anaerobic culture for 3-4 days; when the strain concentration of the second-level strain culture reaches 1×10 8 When the number / ml is above, then inoculate the next level of culture. The preferred light is 1500-2000LUX, so that the cultivation effect is better. The seed tank culture adopts the culture method of aerobic fermentation in the dark, the culture tem...

Embodiment 1

[0041] Example 1: In a cadmium-contaminated soil in Guangdong, 6kg (diluted 30 times) of patented biological preparation was applied per mu, sprayed once every 7 days, and used continuously for 4 times to investigate the repair effect of the biological preparation on soil cadmium pollution . The results showed that after applying the patented bacterial agent, the cadmium content in the plants grown in the soil was reduced by more than 60%, which effectively inhibited the migration of cadmium into the plants and achieved a satisfactory restoration effect.

Embodiment 2

[0042] Example 2: In a certain lead-contaminated soil in Guangdong, 8 kg (diluted 30 times) of patented biological preparations were applied per mu, sprayed once every 7 days, and used continuously for 4 times to investigate the repair effect of the biological preparations on soil lead pollution . The results showed that after the application of the patented bacterial agent, the content of water-soluble lead in the soil decreased by more than 80%, while the content of lead in a stable state increased greatly, indicating that the biological agent can change the form of lead in the soil and reduce it from harmfulness. The high water-soluble state is transformed into a stable state with less harm to the environment, so as to achieve the remediation of lead-contaminated soil.

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PUM

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Abstract

The invention discloses a biological agent capable of repairing heavy metal contaminated soil. The biological agent comprises rhodopseudomonas palustris ATCC17001 strain, N strain and NO.37 strain of rhodopseudomonas in photosynthetic bacteria and rhodobacter sphaeroides ATCC17023 strain, T2 strain and NO.55 strain of rhodobacter. The invention also discloses a preparation method of the biological agent capable of repairing the heavy metal contaminated soil, wherein the production process is a process for amplification culture of microorganisms step by step. The biological agent has the beneficial effects that the endurance capacity of functional microorganisms to heavy metals is high, and the functional microorganisms still maintain the activity and efficiently repair the heavy metal seriously contaminated soil; the biological agent not only can adsorb and accumulate the heavy metals and can convert the forms of the heavy metals, so that the heavy metals are changed into a stable state from a water soluble state which is big in environment hazards; and the biological agent is simple in preparation, wide in application range, low in treatment cost, short in treatment time, complete in treatment and strong in operability, and can be popularized and applied in a wide range.

Description

technical field [0001] The invention relates to the technical field of remediating heavy metal-contaminated soil, in particular to a biological preparation and soil remediation method for remediating heavy metal-contaminated soil. Background technique [0002] Soil is the fertile and loose surface of the earth that can grow plants. It is the basis for human survival and development, and it is also the most basic, important and irreplaceable natural resource for human society. With the acceleration of human industrialization, soil heavy metal pollution has become a major environmental problem affecting human survival, and many world-renowned environmental pollution events have occurred, such as Japan's bone pain disease caused by soil cadmium pollution. Therefore, remediation of soil heavy metal pollution has become a research hotspot in environmental protection around the world. [0003] As a special class of pollutants, heavy metals have significantly different characteris...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/00C12N1/20B09C1/10C12R1/01
Inventor 曹新现吴坚伟
Owner 广州天壤生物科技有限公司
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