76 results about "Cryptocaryon" patented technology

The invention discloses a prevention and control method for cryptocaryon irritans of scatophagus argus. According to physiological and ecological features of the scatophagus argus, the purpose of preventing and controlling the cryptocaryon irritans of the scatophagus argus is met with a combined method of nutrient enrichment cultivation and disease prevention of the scatophagus argus, drug therapy of the cryptocaryon irritans and ecological regulation, wherein therapeutic drugs are copper acetate and zeolite powder combined with copper acetate, furacilin antibiotics or an algae-inhibitory compound and furacilin antibiotics combined with the algae-inhibitory compound. The survival rate of the young scatophagus argus intensively cultivated with the method is greatly increased and reaches more than 95%; the fry survival rate of the diseased scatophagus argus is increased from 0% to 64-88%, so that the economical loss is greatly reduced. The method is remarkable and stable in effect and easy to master, popularize and apply, has the characteristics of economy, good actual effect, low toxicity, easiness for operation and the like, and has an important actual effect for establishing a technical system for disease prevention and control of the scatophagus argus and promoting the development of the breeding industry of the scatophagus argus.

The invention discloses a cyst removal method for controlling fish cryptocaryon irritans. A cloth liner with the strong attaching effect on cryptocaryon irritans brown cysts is screened out mainly and laid on a water bottom of fish infected with the cryptocaryon irritans, the cryptocaryon irritans brown is nourished, falls down and is attached to the cloth liner, the cryptocaryon irritans brown cysts are removed by removing the cloth liner, the life history of the cryptocaryon irritans brown is cut off, and deaths caused when fish is repeatedly infected with the cryptocaryon irritans brown are reduced. By screening the materials, the cloth liner which is resistant to corrosion and free of toxicity and has the strong attaching effect on the cryptocaryon irritans brown cysts is obtained, the method for removing the cryptocaryon irritans brown cysts is used for treating plectorhinchus cinctus infected with cryptocaryon irritans, and the relative protection rate can reach 98%.

The invention discloses an oil-adjuvant vaccine of larvae membrane protein of cryptocaryon irritans, and a preparation method and applications thereof. The preparation method of the vaccine comprises the following steps: (1) collecting live larvae of the cryptocaryon irritans; (2) extracting the larvae membrane protein of the cryptocaryon irritans; and (3) adding PBS in the larvae membrane protein as water phase and mixing No.10 white oil, Span-80 and aluminum stearate as oil phase, emulsifying the larvae membrane protein in the volume ratio of the water phase to the oil phase, which is 3:7, thus obtaining the oil-adjuvant vaccine of the larvae membrane protein of the cryptocaryon irritans. The preparation method of the oil-adjuvant vaccine of the larvae membrane protein of the cryptocaryon irritant is simple and practicable, and the obtained oil-adjuvant vaccine of the larvae membrane protein of the cryptocaryon irritans has high safety, obvious immunoprotection effect and no toxicity to fish, can be used for preventing and treating the parasitic infection of sea fish and can absolutely meet the demand of actual production.

The invention relates to a direct fluorescence labeling method for displaying a cryptocaryon irritans brown microtubule cytoskeleton structure, which comprises the following steps of: preparing a glass slide coated with polylysine; permeating cryptocaryon irritans brown cells through solution PHEM (Postherpetic Erythema Multiforme) containing saponin, and adding PHEM solution for immersion bath; fixing permeated cells through paraformaldehyde solution, and carrying out immersion bath through the PHEM solution, and transferring the fixed cells to the glass slide coated with the polylysine; dripping Triton X-100 water solution on the cells after removing extra fluid, carrying out immersion bath through the PHEM solution, and rinsing through 0.01mol / L of phosphate buffer; rinsing through 0.10mol / L of PBS (Phosphate Buffer Solution) after keeping from light, hatching through the PBS containing 1mu mol / L of fluorescence taxoid at room temperature, removing extra solution, and capping the glass slide; and finally, observing through a microscope and taking photos. The method is simple to operate, has high efficiency, is environmental-friendly and has no pollution.

The invention adopts a method of cryptocaryon irritans locally-infected epinephelus coioides one-sided skin for immunity for the first time, adopts an optimized infection concentration and infection duration time to construct an immune model of the cryptocaryon irritans locally-infected epinephelus coioides one-sided skin for the first time, thoroughly eliminates an adverse effect brought by ''congenital differences'' in the process of local skin mucosal immune response in fish, and provides an ideal immune model for researching a local mucosal immune response law. The construction method forthe immune model mainly comprises the following five steps: (1) collecting live larvae of the cryptocaryon irritans and calculating a concentration; (2) grouping the epinephelus coioides and performing domestication; and (3) performing local skin immunization; (4) measuring infection intensity; and (5) performing treatment on locally-infected fish bodies.

The present invention relates to a compound preparation for treating large yellow croaker irritating cryptocystosis and its application. The compound preparation is composed of 10 wt% to 15 wt% of nitazoxanide and 5 wt% to 7 wt% of copper sulfate , oxytetracycline 25 wt%~30 wt%, dissolving agent 48%~60%, the sum of the above raw materials is 100%. The method of use is: after the diagnosis of cryptocystosis irritant, sprinkle the above compound preparation in an amount of 8~10 g / m3, and soak in the bath continuously for 3 days, which can effectively cure cryptocystosis irritant of large yellow croaker and prevent secondary infection of bacteria . The invention has the advantages of simple preparation method and convenient use, can significantly reduce the death rate of large yellow croaker stimulated by cryptocystosis, and improve the economic benefits of breeding.

The invention belongs to the field of fish disease prevention and control and in particular relates to a method for controlling cryptocaryon irritans diseases of fishes by using ozone. According to the method, by virtue of the characteristic that larvae of cryptocaryon irritans are centrally incubated at night, ozone is introduced into a breeding water body in a larva incubation period, and the ozone concentration is kept within a lowest concentration range which is capable of effectively deactivating the larvae of the cryptocaryon irritans; and by cutting off the life history of the cryptocaryon irritans, the purposes of treating and preventing the cryptocaryon irritans diseases can be achieved. By adopting the method, experiments that the larvae of the cryptocaryon irritans are treated with seawater of different ozone concentrations show that the lowest effective deactivation concentration is 0.1-0.15mg / L; and trachinotus ovatus is adopted for effect verification experiments, resultsshow that 411.4+14.7 cysts are generated by each fish on average when the ozone concentration is 0mg / L, 0.4+ / -0.1 cyst is generated by each fish on average when the ozone concentration is 0.1mg / L, and 0 cyst is generated by each fish on average when the ozone concentration is 0.15mg / L. Results show that when the ozone concentration is 0.1-0.15mg / L, the larva infection capability of the cryptocaryon irritans can be remarkably reduced (P is less than 0.05), and the cryptocaryon irritans diseases can be effectively controlled.