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Breeding method of pseudosciaena crocea resisting cryptocaryon irritans based on whole genome selection

A technology that stimulates Cryptocaryonis and the whole genome, which is applied in biochemical equipment and methods, fish farming, microbial measurement/inspection, etc., can solve the problems that parents cannot perform resistance evaluation, shorten the breeding period, and reduce the economic cost. loss effect

Active Publication Date: 2021-01-29
XIAMEN UNIV
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  • Claims
  • Application Information

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Problems solved by technology

[0004] The purpose of the present invention is to provide a breeding method for excellent strains of large yellow croaker resistant to irritating Cryptocaryoniasis based on whole genome selection, which effectively solves the problem that the parents cannot perform resistance evaluation in the breeding of complex traits.

Method used

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  • Breeding method of pseudosciaena crocea resisting cryptocaryon irritans based on whole genome selection
  • Breeding method of pseudosciaena crocea resisting cryptocaryon irritans based on whole genome selection
  • Breeding method of pseudosciaena crocea resisting cryptocaryon irritans based on whole genome selection

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Embodiment Construction

[0054] The present invention will be further illustrated and described in detail below in conjunction with the accompanying drawings.

[0055] see Figure 1~5 , the embodiment of the present invention includes the following steps:

[0056] (1) Establish a reference group;

[0057] ① In December 2017, in a cage culture area in a sea area of ​​Fujian, 500 healthy 2-year-old sexually mature large yellow croaker broodstock from various sources were selected from various fish rafts, and the source of broodstock was extensive to ensure its genetic diversity.

[0058] ②The broodstock were not selected through stimulating Cryptokaryon worm attack, and after intensive cultivation, they induced labor and reproduced offspring.

[0059] ③The descendants of this batch of broodstock are the reference group, and the reference group is cultivated according to the commercial fish breeding system.

[0060] (2) Measure the resistance traits of the reference population;

[0061] ① In mid-June...

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Abstract

The invention discloses a breeding method of pseudosciaena crocea resisting cryptocaryon irritans based on whole genome selection, and belongs to the field of animal disease resistance breeding. The method comprises the steps of establishing a reference group; performing resistance trait measurement on the reference group; establishing a breeding group; carrying out genotyping on the reference group and the breeding group; establishing an optimal whole genome selection model; estimating a genomic breeding value GEBV of the breeding group; selecting individuals, with the GEBV ranked in the front, of the breeding group as parent fishes according to a certain selection intensity, and propagating to generate a first filial generation resisting the cryptocaryon irritans; and conducting cryptocaryon irritans toxicity attack verification on the first filial generation with resistance. The insect resistance is obviously improved; and the estimation accuracy of the genomic breeding value of thebreeding group is improved, the breeding period is greatly shortened, high-resistance offspring can be obtained within one generation, the economic loss of the cryptocaryon irritans to the pseudosciaena crocea breeding industry is reduced, reference and basis are provided for disease-resistant breeding of other fishes, and the method has a wide application prospect.

Description

technical field [0001] The invention belongs to the field of animal disease-resistant breeding, and in particular relates to a large yellow croaker anti-irritant Cryptocaryoniasis breeding method based on whole-genome selection, which utilizes whole-genome selection technology to breed large yellow croaker anti-irritant Cryptocaryoniasis. Background technique [0002] Large yellow croaker (Pseudosciaena crocea), belonging to the order Perciformes, the family Sciaenidae, and the genus Larimichthys, is mainly distributed in the southwestern Pacific Ocean, that is, the southeastern coastal area of ​​China. The wild resources of large yellow croaker tend to be exhausted due to overfishing, but after scientific and technological workers broke through the artificial breeding of large yellow croaker, its breeding scale has increased year by year, and now it has become the main seawater species cultured in the southeastern coastal provinces of China, especially Fujian Province. It i...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01K61/10A01K67/027C12Q1/6888
CPCA01K67/027A01K2207/00A01K2227/40A01K61/10C12Q1/6888C12Q2600/124Y02A40/81
Inventor 赵吉徐鹏柯巧珍郑炜强
Owner XIAMEN UNIV
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