Breeding method of large yellow croaker resistant to irritating Cryptocaryoniasis based on genome-wide selection

Abstract

The invention discloses a large yellow croaker anti-stimulatory cryptocystosis breeding method based on whole-genome selection, belonging to the field of animal disease-resistant breeding. Methods: establish a reference population; measure the resistance traits of the reference population; establish a selective breeding population; genotype the reference population and the selective breeding population; establish the best genome-wide selection model; Estimate; Select the individual with the highest GEBV value in the breeding group as the broodstock with a certain selection intensity, and breed to produce the first generation of anti-stimulatory Cryptocaryonis; the anti-stimulatory generation is verified by the challenge of Cryptocaryon stimuli. Significantly improved insect resistance. It improves the estimation accuracy of the genome breeding value of the selected breeding population, greatly shortens the breeding period, and can obtain highly resistant offspring within one generation, reducing the economic loss caused by stimulating Cryptocaryonia to the large yellow croaker breeding industry, and also It provides a reference and basis for disease-resistant breeding of other fishes, and has broad application prospects.

Description

technical field [0001] The invention belongs to the field of animal disease-resistant breeding, and in particular relates to a large yellow croaker anti-irritant Cryptocaryoniasis breeding method based on whole-genome selection, which utilizes whole-genome selection technology to breed large yellow croaker anti-irritant Cryptocaryoniasis. Background technique [0002] Large yellow croaker (Pseudosciaena crocea), belonging to the order Perciformes, the family Sciaenidae, and the genus Larimichthys, is mainly distributed in the southwestern Pacific Ocean, that is, the southeastern coastal area of ​​China. The wild resources of large yellow croaker tend to be exhausted due to overfishing, but after scientific and technological workers broke through the artificial breeding of large yellow croaker, its breeding scale has increased year by year, and now it has become the main seawater species cultured in the southeastern coastal provinces of China, especially Fujian Province. It i...

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Problems solved by technology

[0004] The purpose of the present invention is to provide a breeding method for excellent strains of large yellow croaker resistant to irritating Cryptocaryoniasis based on whole genome selection, which effectively solves the problem that the parents cannot perform resistance evaluation in the breeding of complex traits.

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