253 results about "Asexual reproduction" patented technology

The invention provides a method for quickly reproducing bletilla striata seedlings, which comprises the following steps: 1) using mature seeds of bletilla striata as explants; 2) sterilizing the seeds and inoculating the seeds to a germination culture medium to allow the seeds to germinate in about 7 days, cutting protocorms formed in about 14 days, and transferring the cut protocorms onto a protocorm proliferation culture medium; 3) transferring the protocorms onto a cluster bud induction and proliferation culture medium; and 4) cutting the cluster buds, allowing the cluster buds to propagate massively, transplanting 3-centimeter seedlings grown from the cluster buds onto a rooting and seedling strengthening culture medium, and transplanting the seedlings when the seedlings grow to 7 to 10 centimeter. The method solves the problems of virus carrying seedlings, deterioration of variety, low effective yield and the like of the conventional bletilla striata asexual reproduction. When the method is used, a large number of asexual reproduction virus-free seedlings can be obtained under a manual control condition; compared with the conventional reproduction method, the yield is improved by 30 percent; and thus, the method has an active significance for reliving market contradiction between supply and demand for bletilla striata.

The invention relates to a propagation method in the field of vegetative propagation, specially relates to a cluster bud propagation method of Paris polyphylla rhizomatic of Paridis. The invention is to provide a method with the advantages of simple operation, low cost, high propagation coefficient, rapid plumule growth, good seedling growth vigor, and no influence to the medicinal value of the propagating material to solve the problem of hard propagation of Paris polyphylla. The cluster bud propagation method of Paris polyphylla rhizom comprises selecting propagating material, processing method of the propagating material, processing time of the propagating material which can be performed all year around, inducing circumstance of cluster bud and managing, transplanting the seedling of cluster bud. Compared with the present technology, the invention has the advantages of simple operation, low cost, high propagation coefficient, good seedling growth vigor, and no influence to the medicinal value of the propagating material.

The invention discloses a method for breeding tetraplont locust own-rooted plantlets by means of hardwood cutting; in the invention, a root nursery is established by planting the own-rooted plantlets,well-bred roots are collected as breeding materials, and a plant growth regulator is added in a matching manner to realize asexual reproduction of the tetraplont locust in large scale. The method obtains the tetraplont locust own-rooted plantlets by tissue culture or hardwood cutting; the own-rooted plantlets are planted to form the root nursery; and the fertilizer and water management of the root nursery is strengthened to accelerate the growth of the plantlets. After two years, multitudinous root segments can be provided every year; the robust root systems of which root diameters reach 0.3cm are screened in the root system collection in spring, then the root systems are pruned into root segments with a length of 5-6cm and planted in a seeding nursery; and most plantlets grew in currentyear can be as high as 1.0m and the ground diameter can reach 0.8cm, which can be used for forestation. The method for breeding tetraplont locust plantlets has the advantages of simple operation, reasonable design and remarkable effect and greatly improves the breeding efficiency of well-bred tetraplont locust plantlets.

The invention relates to the technical field of traditional Chinese herbal medicine cultivation, in particular to a herbahouttuyniae cultivation method, which comprises the following steps of: 1) determining a planting time: planting seedlings at a period from the middle ten days and last ten days of September to the first ten days of November every year; 2) determining a planting mode: selecting high-stalk crops for interplanting; 3) preparing the seedlings: conducting asexual reproduction by using subterraneous stems of herbahouttuyniae as reproduction materials; 4) planting the seedlings: directly placing the seedlings on compartment surfaces and covering the compartment surfaces with rice straw or maize stalks after the seedlings are planted; 5) conducting field management: removing weed at the growth period of the herbahouttuyniae, removing ill herbahouttuyniae plants, weak herbahouttuyniae plants and non-herbahouttuyniae crops during weeding, and making sure that the herbahouttuyniae is not damaged; and during top dressing, using farm manure as main fertilizers and chemical fertilizers as auxiliary fertilizers, paying attention to reasonable drainage and irrigation and keeping soil wet at the entire growth period of the herbahouttuyniae; and 6) harvesting at a proper time and the like. By adopting the herbahouttuyniae cultivation method, the yield of the herbahouttuyniae is high, the damage by diseases and insects is reduced, the herbahouttuyniae diseases such as southern blight and zonate spot can be effectively prevented and controlled, and the yield of the herbahouttuyniae and the mass content of medicinal components in the herbahouttuyniae are improved to the utmost extent.

The invention relates to a forest tree seedling breeding technology and in particular relates to a method for suppressing browning of a carya cathayensis sarg explant. The method is characterized by selecting a carya cathayensis sarg stem with axillary buds as an explant in spring, disinfecting the explant, then inoculating the explant into a culture medium to which catechins and anti-browning agents are added, and firstly culturing a tissue culture vessel in the low temperature dark environment and then transferring the tissue culture vessel to the routine culture environment at the initial stage of inoculation. The method can conduce to obvious reduction of the browning rate of the carya cathayensis sarg explant and improvement of inductivity of the carya cathayensis sarg explant, is simple and convenient to operate, has a better browning suppression effect, and provides a technical support for tissue culture and asexual reproduction of carya cathayensis sarg.

The present invention relates to methods for promoting somatic embryogenesis from a tissue or organ of a plant, by overexpressing a Wuschel gene in said tissue or organ. In one embodiment, such overexpression can be used as a silent selectable marker for transgenic plants. In another embodiment, such expression can be used to confer apomixis to a plant. In another embodiment, such overexpression can be used to create haploid plants, which can be used to produce dihaploid plants.

The invention relates to an asexual reproduction method of cutting-seeding raising cyclocarya paliurus branches to solve the seedling technical problem of cyclocarya paliurus and to efficiently cultivate clone feedings. The method includes the following steps, firstly choosing un-entirely lignification branches from robust cyclocarya paliurus plants and cutting sections with two leaf buds as cuttings, wherein the biology bottom of a cutting is flat and 2 to 3 leaves are left on the upper portion, secondly immersing the lower end of the cuttings in rooting solution for 20 to 100 minutes, thirdly inserting cuttings treated by rooting agent into soil matrix with a depth of 3 to 3.5 cm and spraying with 80% by wt carbendazim until the upper leaves completely wetted after cutting, and fourthly shedding the cut branches with black sunshade nets, sterilizing and moistening the cuttings by spraying carbendazim every two days at the first week and later moistening by spraying water every two days. The cutting-seedling raising method of cyclocarya paliurus branches is simple and practical in operation, short in seedling period and high in cutting rooting rate, and is suitable for large scale cutting seedling.

An artificial reproduction method for drynaria includes asexual production and spore reproduction. Said asexual reproduction includes such steps as taking its rhizome segment with 3-5 bud points and 3-5 sporophyllary leaves, fixing it to tree or mountain rock, covering it by bryophyte, spraying water and management. Said spore reproduction includes such steps as sterilizing the burnt earth, immersing in cold boiled water, loading the sporanges in bag, drying in air, preparing the suspension of sporanges, spraying it onto said burnt earth, optical culture to obtain gametophytes, transplanting, culturing to obtain sporophytes, and transplanting them on tree or mountain rock.

The invention discloses a wild culture-imitating method for rheum officinale baill. The method is characterized by planting seedlings subjected to sexual reproduction and seed blocks subjected to asexual reproduction in an adret sloping field with a high altitude of 1,500 to 1,800 m and a gradient of less than or equal to 30 degrees and performing extensive management by a wild-imitating method, wherein the seedlings subjected to sexual reproduction is cultured in an adret flat field with a low altitude of 800 to 1,000 m; during seedling culture, the thousand seed weight is more than or equal to 12.9 g, the moisture content is lower than 6.51 percent and the purity is over 98.9 percent; after immersion and germination acceleration, the seeds are sown on a seedbed; after the seedlings grow to be 10 centimeters high and the ground diameter is more than 2 centimeters, the seedlings are transplanted; the transplanting time is the first ten days of April every year; farmyard manure subjected to innocent treatment is applied one time per year; and after three years, the rheum officinale baill can be harvested. The invention provides a practicable method for realizing large-area standard plantation of the rheum officinale baill. By the method, the yield and the quality of the rheum officinale baill can be improved; and all the officinal active ingredients of the product are over 1.5 percent.

The present invention is directed to the seed-to-seed perpetuation of hybrid vigor and other traits through apomixis (asexual seed formation) in flowering plants (angiosperms). More particularly, to predictable methods for producing, from sexual or facultatively-apomictic plants, progeny plants that express an increased percentage of apomictic seed set or one or more elements of apomixis. This invention uses: plant cyto-embryology procedures to identify and select a plant or group of plants that possess appropriate genetic variability for initiation times and durations of megasporogenesis (female meiosis), embryo sac formation, egg and central cell formation and maturation, fertilization, embryony and endosperm formation; plant breeding procedures to produce numerous and divergent genetically-recombined early to late generation progeny such that embryo sac formation preempts megasporogenesis and embryony preempts fertilization; and plant cyto-embryology or progeny test procedures to select segregant plants that express an increased frequency of one or more elements of apomixis.

The invention relates to a cultivation method of the triploid salvia, the steps of the invention are that: not completely ripe good salvia seeds are collected to put into the 1 / 2MS culture medium with colchicine for mutagenesis cultivation; then the seeds are transferred into a normal 1 / 2MS culture medium for cultivation, the root tip of the seedling is fetched to perform the ploidy identification of the chromosome after the preceding process of the chromosome, and the seedling with a chromosome quantity of 2n=4x=32 is the tetraploid salvia; the tetraploid salvia is taken as a female parent and the diploid salvia is taken as a male parent to perform the crossbreed and the obtaining seed is a triploid salvia plant. The nutrient organs such as the root, stem and leaf and so on of the triploid salvia plant obviously become bigger and present remarkable crossbreed advantages, and the root medicinal material output of the triploid salvia is increased by over 30 percent. Moreover, the triploid salvia has flowers, but no seeds, thus decreasing the nutrition consumption and the crossbreed advantages of the triploid salvia can be kept over 10 years through the asexual reproduction, which enhances the disease resistance and stress resistance with strong applicability, thus being applicable to planting in every part of the country. Besides, the crossbreed advantages produced by the novel variety triploid production are able to improve the output of the salvia medicinal material planting in a large scale.

The invention discloses a high-yield Magnolia officinalis propagation method, which relates to the technical field of plant propagation. The propagation process includes seed selection, seed treatment, selection and retention of female parent saplings, establishment of collection gardens, tree planting screening, and asexual propagation; it has a propagation process The invention has the characteristics of being simple, capable of breeding magnolia saplings with good resistance, increased weight of effective parts, and high active ingredient increment, and can be used for breeding high-yield magnolia saplings and other plants.

The invention discloses technology for breeding heartleaf houttuynia herb seedlings, and relates to the fields of biotechnology and crop cultivation. The technology comprises seed collection, seed storage, seed sterilization, seed low-temperature treatment, seed germination, seedling cultivation and other processes, wherein the mature and dry heartleaf houttuynia herb seeds are sterilized by 70 percent ethanol and 0.1 percent mercuric chloride, are washed by sterile water for 4 to 5 times, and are treated at low temperature of between 1 and 4 DEG C for 7 days; and the seeds are sowed on an MS minimal medium and are transplanted to a seed bed for culturing seedling 14 days after the seeds are germinated in an illumination incubator. The technology treats the heartleaf houttuynia herb seeds at low temperature, changes the physiological status of the seeds, and promotes the seeds to germinate and grow to form seedlings. The seed breeding method can also avoid the problem of plant diseases and insect pests due to the fact that insect sources of a stock plant are easily brought to the seedlings by rhizome asexual reproduction, and can reduce planting cost simultaneously.

The invention discloses a method for regenerating a plant by use of the callus of camellia japonica, belonging to the field of biotechnology. The method is characterized by comprising the following steps of: collecting the fruits of camellia japonica in early September, and disinfecting and cleaning; removing the inner and outer seed coats of seeds, and inoculating to a callus induction medium; transferring the material to a callus transition medium in the 25th -28th day; after the callus turns from light yellow into yellow with a little red, transferring the material into a callus differentiation adventitious bud medium; after the material is formed and bud sticks of 3-4cm grow, soaking the base part in IBA, and transferring to a paper bridge of an MV liquid medium to induce rooting; starting in the 20-22 days to grow transparent radicles with a little red; and then culturing for a certain period of time so that the radicels extend and are gradually lignified. The method for regenerating a plant by use of callus of camellia japonica, disclosed by the invention, provides a process with a relatively short period and a relatively high reproduction coefficient to large-scale asexual reproduction of camellia japonica, and lays a foundation for the molecular breeding of camellia japonica.

The invention discloses a culture medium for promoting an adventive root of a woody plant to root and grow and an application of the culture medium. The culture medium comprises the following components: inorganic salt A, inorganic salt B, an organic compound, 50-150mg / L water-soluble carbon nano tubes, 0.5-1.5mg / L indolebutyric acid, 100mg / L 2-(N-morpholine) ethanesulfonic acid, 30000mg / L cane sugar and 5000mg / L agar. The culture medium provided by the invention is more capable of promoting the growth of the adventive root. The largest difficulty of asexual reproduction of the woody plant lies in the rooting and the growth of the adventive root, the culture medium has the same promotion effect for the rooting of the adventive root of brassaia actinophylla and the rooting and the growth of plants such as honeysuckle, blueberry, small kiriko, swida wilsoniana, zanthoxylum dissitum and the like.

The invention discloses a method for breeding an improved variety of scrophularia ningpoensis Hemsl., and solves problems that due to long time asexual reproduction of scrophularia ningpoensis Hemsl. in the prior art, virus accumulation is serious, and the yield and the quality are reduced. The method comprises the following steps: 1), screening plants, wherein the pest-free plants are selected; 2) pre-treatment and disinfection, wherein scale-peeled buds are soaked with the 20-time sodium hypochlorite solution for 8-10 min; 3) stem tip peeling, wherein each stem tip with 1-2 leaf primordium are cut; 4) cultivation, wherein the stem tips are placed in a culture medium for cultivation; 5) exercised seedling transplantation, wherein the seedlings are cultivated in the sun, then the seedlings are transplanted to a matrix to be cultivated for 15 days, and finally the seedlings are transplanted to a field. Compared with the conventional asexual reproduction method for scrophularia ningpoensis Hemsl., the yield and the quality of the scrophularia ningpoensis Hemsl. seedlings cultivated by the method are greatly improved.

The invention relates to a grafting method capable of promoting asexual reproduction of populus euphratica. The method comprises the following steps: (1) irrigation and pest control before grafting; (2) selection of a rootstock: selecting annual Populus bolleana as the rootstock, wherein a tree trunk of the Populus bolleana is straight from top to bottom, and the Populus bolleana is vigorous in growth and full in scion; (3) selection of populus euphratica scion: selecting a bud as the populus euphratica scion, wherein the middle portion of a seeding branch is full, the seeding branch has 4-6 buds and lengths of the buds is 8-12 millimeters; (4) cutting a cross-shaped incision on a rootstock Populus bolleana trunk at a height of 1-1.2 meters with a grafting knife, and levering the incision; (5) clipping the lower end of a populus euphratica scion lower bud into a rhombus and obtaining a clipped populus euphratica scion; (6) inserting the clipped populus euphratica scion into the incision position around the Tomb-sweeping Day and binding tightly, and then the grafting is finished; (7) irrigating and fertilizing timely after the grafting is finished; and wiping off all the buds ready to sprout when the populus euphratica starts to sprout, and spraying pesticides when 3-4 true leaves grow on the populus euphratica seedling. The grafting method capable of promoting the asexual reproduction of the populus euphratica can effectively stop a populus euphratica population from being weak in regeneration and aging.

The invention discloses a non-tube cutting seedling method of tissue subcultured buds of zenia. The two steps of non-tube rooting of tissue subcultured buds and cutting seedling are combined into one. The tissue subcultured buds of zenia are bred by a conventional method, and then the tissue subcultured buds of zenia are selected as cuttings and are treated by a root promoter and cut into a red subsoil matrix, and seedlings are cultured to obtain forestation wood seedlings for planting. According to the method, the tissue subcultured buds of zenia are directly used as the cuttings for non-tube cutting, the breeding speed is fast, and seasonal and geographical restrictions do not exist; and meanwhile, the two steps of non-tube rooting and cutting seedling are combined into one, the method is simple, the operation is easy, rooting starts after cutting culture for 15 to 20 days, the seedling survival rate is high, and the rooting survival rate is 85% to 95%. The method overcomes the defects of the traditional zenia asexual reproduction method, the seedling cycle is shortened, the seedling survival rate is improved, the seedling cost is reduced, and the method is favorable for accelerating the development of asexual seedling of zenia.

The invention relates to the use of monoterpenes, sesquiterpenes and / or diterpenes in addition to derivatives thereof for inhibiting the asexual reproduction of fungi and filter materials, building material, auxiliary building materials, textiles, fur, paper, skins or leather, and also washing agents, cleaning agents, rinsing agents, handwashing agents, agents for handwashing dishes, dishwasher agents, and agents for building materials, auxiliary building materials, textiles, fur, paper, skins or leather containing monoterpenes, sesquiterpenes and / or diterpenes or derivatives thereof.

The invention relates to a method for obtaining hydranths of mastigias papua, and belongs to the field of artificial feeding and propagating of ornamental marine animals. The method comprises the steps of: culturing parents in a manner of completely being away from light, wherein the culturing water temperature is 25-28 DEG C, enabling the cultured parents to be fertilized, and when fertilized eggs grow into planula larvas floating and swimming on a water surface, taking out the parents; shifting the planula larvas into fresh seawater with the same temperature, at the same time, putting attaching pieces in the fresh seawater, wherein the putting density is that 400-600 planula larvas and two attaching pieces are put in each one liter of a container, and culturing the planula larvas at a constant temperature; when the planula larvas grow into the hydranths with 4 feelers in an attaching manner, beginning to feed the hydranths with artemia nauplii every day, after feeding the hydranths with the artemia nauplii for 2.5-3.5 hours, completely changing water, when the hydranths grow to have 16 feelers, controlling the culturing temperature to be 20-27 DEG C, and extending the number of the hydranths through asexual reproduction. According to the method disclosed by the invention, the hydranths of the mastigias papua can be cultured in batches, and the requirements of showing, enjoying and researching the hydranths of the mastigias papua can be met. The breeding blank of the mastigias papua is filled.

The invention relates to the technical field of asexual reproduction of seaweed artificial seedlings, and particularly relate to a seedling cultivation method for Sargassum vachellianum greville by using tissue culture. The method comprises acquiring fully grown wild Sargassum vachellianum greville with immature receptacles, holding culture, accelerating maturation, obtaining oosperms for static cultivation, and obtaining seedlings; segmenting the blades of seedlings of low age in days and the holdfasts of seedlings of low age in days for tissue culture, and obtaining regenerated seedlings; separating the regenerated seedlings from tissue blocks, culturing in vitro, obtaining complete seedlings, and holding culture; segmenting the blades of complete seedlings in holding culture again for tissue culture, and obtaining regenerated seedlings again; and finally separating the regenerated seedlings from tissue blocks, culturing in vitro, inducing the bases of the regenerated seedlings to regenerate holdfasts to form complete seedlings, and obtaining complete seedlings for another tissue culture. The blades and the holdfasts of the regenerated seedlings obtained by using the method of the invention can be used as regeneration sources for continuous use for many times, and are large in amount and easy for storage; the reproduction time is short, the acquisition of seedlings are not limited by objective conditions such as seasons, ecological conditions and the release amount of generative cells.

The invention relates to a method for cultivating gardenia jasminoides, which belongs to the field of plant tissue culture, in particular to a method for cultivating the gardenia jasminoides and a non-pollution cultivation method thereof. The method comprises the following steps: disinfecting and cleaning the outer planting body of the gardenia jasminoides; inducing and differentiating the callus; proliferating the adventitious buds; inducing the adventitious roots; acclimatizing; and executing the non-pollution cultivation to realize the experiment purpose of the invention. The invention has the following beneficial effects: the invention obtains a large number of tissue culture seedlings to meet the planting needs, and overcomes the pollutions of pesticide and heavy metal in natural wood area. The survival rate is 95.5%, the weight of single plant is twice as the wild and asexual reproduction drug materials in the same year, and the effective components is higher than the wild drug materials in the same year. The invention meets the needs of original ingredients of pharmaceutical industries, not only cultivates in batches, but also plants the tissue culture seedlings in all areas, obtains the non-pollution drug materials two years in advance, and has practical value.

The invention provides a grape planting method. The grape planting method comprises the steps of cutting shoot selecting, seedling land preparing, transplanting and managing. According to the grape planting method, pit manure, organic fertilizer and inorganic fertilizer are combined together to be used, the organic fertilizer is taken as a main fertilizer source, and nutrient substances needed in the grape growing process are guaranteed by supplementing the inorganic fertilizer; through fine management on grape seedlings, pest and disease damage is prevented from occurring, and the using variety and number of pesticides are decreased; cutting-shoot asexual reproduction is adopted, material obtaining is precise, management is scientific and reasonable, and the taste and plumpness of grapes are effectively kept while the grape yield is not decreased.

The invention provides a hibernating conservation asexual reproduction method for jellyfish hydranth. The method is characterized in that when normal cultivation of young jellyfish is finished in early summer every year, an enough quantity of jellyfish intestinal-shape young fingerlings are purchased and subjected to conservation, illumination, feeding, trace-amount inflation, water changing, asexual reproduction and development under the condition of low temperature. The method improves the efficiency and benefit of cultivating jellyfish fingerlings by reducing or avoiding a sexual reproduction process of fingerling cultivation, has a simple structure, reduces production cost, and is suitable for extensive popularization.

The use of ortho-phenylphenol and / or derivatives thereof to inhibit the asexual reproduction of fungi, and to filter media, construction materials, construction adjuvants, textiles, furs, paper, hide, or leather. Also, washing agents, cleaning agents, rinsing agents, hand washing agents, hand dishwashing agents, automatic dishwashing agents, and agents for finishing construction materials, construction adjuvants, textiles, furs, paper, hides, or leather, that contain ortho-phenylphenol and / or derivatives thereof,

An asexual reproduction method for Chinese prickly ash tree features that the bud of domestic Chinese prickly ash tree is grafted to wild one as stock at the position which is 15-20 cm far from ground in spring or autumn. Its advantages include simple operation, high survival rate, early fruiting, high yield, and high quality of fruit.

A method provided by the present invention is applicable to the aspects such as sargassum fusiform germplasm resource analysis, artificially cultured sargassum fusiform group line classification, elite seed and pedigree seed near-source genetic relationship analysis and manual seedling screening by using sexual reproduction of sargassum fusiform. The method mainly comprises the operating steps of sargassum fusiform history feature and sargassum fusiform mature sporophyte morphological feature main component morphological measurement, sargassum fusiform mature sporophyte morphological feature correlation mathematical model analysis, sargassum fusiform mature sporophyte morphological feature main component mathematical model analysis, sargassum fusiform mature sporophyte morphological feature main component cluster mathematical model analysis, and sargassum fusiform mature sporophyte line differentiation and classification determination and the like. The present invention may provide a scientific differentiation and classification method for the aspects such as sargassum fusiform germplasm resource investigation, sargassum fusiform mature sporophyte group diversity analysis, sargassum basic scientific research and experimental sample screening, sargassum fusiform introduced seed morphological feature stability analysis, manual elite seed differentiation and screening by using sexual reproduction of sargassum fusiform and asexual reproduction excellent plant rhizoid storage.

The invention discloses an agrobacterium-mediated spirodela polyrhiza stable transformation system establishment method, which comprises the following steps: A, inducing calli of spirodela polyrhiza; B, connecting a target gene with a T vector; C, connecting the target gene with a plant expression vector; D, transforming the plant expression vector in agrobacterium tumefaciens LBA4404; E, preparing an infective bacteria solution; F, co-culturing the calli and the agrobacterium tumefaciens; G, performing regeneration and selective culturing: determining that the target gene is successfully integrated into a genome of the spirodela polyrhiza if a GUS (glucuronidase) staining result of thalli is blue, and determining that a part which is stained to be blue by thallus tissues is a part expressed by the target gene. The target gene is inserted into the plant expression vector and then transferred into the agrobacterium tumefaciens LBA4404 for callus infection, and the concentration of antibiotics is finally controlled to screen a positive plant to obtain transgenetic spirodela polyrhiza, and an established transformation system can still ensure continuous and stable inheritance after asexual reproduction of the spirodela polyrhiza, and has bio-safety and development and application value.

The invention provides an imitation wild gastrodia elata cultivation method, and belongs to the field of Chinese herbal medicine planting. The method comprises the following steps that 1, a bacteria bed is arranged; 2, a fungus material is laid flat, and amillaria mellea cultispecies are inoculated; 3, endophytic fungus cultispecies and gastrodia elata capsules are mixed, and seed dressing endophytic fungi are obtained; 4, a layer of seed dressing endophytic fungi are laid flat on the fungus material, a layer of leaves are scattered, and earthing is conducted; 5, the bacteria bed is dug out, white hemp is fixedly planted at the two ends of the fungus material, a bacteria introduction material is placed, and earthing is conducted; 6, harvesting is conducted. Accordingly, when gastrodia elata is planted, sexual propagation and asexual reproduction can be conducted synchronously, the land utilization rate is increased, the cost is reduced, the yield per mu of dried arrow hemp products is187.2 kg each year, the output value is 42,000-66,000 yuan, compared with a traditional planting method, the yield per mu is 21,000-30,000 yuan higher, and the economic value is considerable.

The invention relates to the technical field of asexual reproduction of crops, in particular to a method for rapidly multiplying seeds by cutting cotton nodes. During the cotton growth and development season, the main stem node or fruiting branch node of the cotton plant is used to cut cuttings with a length of 5 to 8 cm, and one node and one leaf are used for cutting; The soil is disinfected, and the water content is subject to the saturation of the soil surface. Cover the top of the cuttings with an arch film, and seal them with soil around them to maintain temperature and humidity. After 20 days, it can be transplanted to the field. Field cultivation is the same as conventional cultivation. The cutting seedlings cultivated by this method have a transplanting seedling rate of about 98%, which can save costs.