Method for breeding improved variety of scrophularia ningpoensis Hemsl.
A technology of Scrophulariaceae and improved varieties, applied in the field of breeding of Scrophulariaceae improved varieties, can solve problems such as virus accumulation, and achieve the effects of increasing the rooting rate and the number of roots, dark green leaves, and robust plant growth.
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[0020] A method for breeding fine varieties of Scrophulariaceae in this scheme is characterized in that it comprises the following steps:
[0021] 1) Plant screening: when Scrophulariaceae is mature, select plants without pests and diseases;
[0022] 2) Pretreatment and disinfection: The screened Scrophulariaceae take the sub-buds wrapped in white scales, without cracks, and the length is longer than 3cm, wash them with clean water, peel off the scales layer by layer, rinse the sub-buds with 70% alcohol for 30s, Then soak in 20 times sodium hypochlorite solution for 9 minutes, and rinse 4 times with sterile water;
[0023] 3) Stem tip stripping: Place the shoot tip of the seed buds treated in step 2) under a microscope, soak them in alcohol, then burn and cool them with tweezers and scissors, peel off the outer leaves of the seed buds, and cut out 0.2mm strips There are 2 leaf primordium shoot tips, and then wash it 3 times with sterile distilled water;
[0024] 4) Cultivati...
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