The invention relates to a method for culturing a mother spawn, a liquid spawn and a solid spawn of phlebopus portentosus. The method is characterized in that: raw materials such as potato, sucrose, glucose, agar, yeast extract, MgSO4, KH2PO4, rubber wood timber dust or other weed tree dust, grains and water are used to prepare culture media of the mother spawn, the liquid spawn and the solid spawn according to a certain proportion; and according to the culture method from the mother spawn, the liquid spawn to the solid spawn, the phlebopus portentosus mother spawn and liquid spawn are firstly cultured, and then the liquid spawn is inoculated on the solid spawn culture medium so as to culture the solid spawn. The culture mediums are particularly suitable for the culture of the phlebopus portentosus mother spawn, the liquid spawn and the solid spawn. The method can be used to carry out the rapid culture of the phlebopus portentosus spawns from the mother spawn, the liquid spawn to the solid spawn.