422 results about "Shoot apex" patented technology

The invention provides a method for rapidly reproducing high quality germchit with dendrobium officinale, which is characterized in that: (1) folded sprouts of the dendrobium officinale are taken and sterilized, and stem apexes are inoculated to a solid culture medium to establish a non-bacterial system; (2) the stem sections or small basal tissue blocks of the young seedlings of the non-bacterial system are cut for inducing protocorm based on suitable hormone combination and a culture medium; (3) the protocorm is cultured alternatively on liquid and solid subculture mediums for rapid multiplication to form protocorm masses; (4) the protocorm masses are cut apart and transferred on a solid planting medium for disuniting plants into small seedlings; (5) the small seedlings are transferred on a solid strong seedling culture medium for strengthening the seedlings and taking roots to culture complete plants; (6) the non-bacterial strong seedlings are fixedly planted in suitable seedling adapting substrate to obtain the high quality germchit. The method can be used in large scale industrialization and the explants have the advantages of high soil removal efficiency, high multiplication rate, low variability, strong germchit, good quality, high survival rate after being transplanted, strong growth potential, etc.

A "Jiuxiang" strawberry stem tip rapid breeding method by tissue culture and virus removal is disclosed. The rapid breeding method includes: explant disinfection, stem tip tissue virus removal and culture, multiplication culture, rooting culture, seedling hardening and transplanting. A stem tip tissue culture method is adopted to breed "Jiuxiang" strawberry virus-free seedlings, thus ensuring that all the excellent characteristics of parents are stably inherited, and therefore influences of outside conditions can be prevented, and the rapid breeding method can be performed at all seasons, thus saving the seedling culture land, and reducing the production cost. A large quantity of good test-tube plantlets can be produced in a short period of time. Large-scale production can be performed to provide "Jiuxiang" strawberry seedlings with a large quantity and good quality for the market so as to meet market needs. The "Jiuxiang" strawberry virus-free seedlings prepared by the rapid breeding method are high in growth vigor, disease-resistant and increased in big-fruit yield. The yield can be increased by 30-50%.

The present invention belongs to the field of agricultural seedling culturing technology. The strawberry stem apex culturing process to produce detoxicated seedling includes the following steps: 1. taking strawberry terminal bud in spring or autumn and sterilizing; 2. taking stem apex of 0.2 mm size under binocular anatomical lens and culturing in compounded 0.5 mg / L concentration MS+BA culture medium to grow cespitose bud; 3. inoculating cespitose bud to 0.2 mg / L concentration MS+BA culture medium for culturing; 4. cutting down giant strawberry seedling from the cespitose bud after proliferation culture, and transferring to 1 / 2MS culture medium for rooting while culturing the rest in the 0.2 mg / L concentration MS+BA culture medium; and 5. domesticating test tube seedling via transplanting the rooted seedling to seedling bed. The said process has 30 % raised detoxicated seedling producing efficiency and obviously improved quality compared with convenient seedling culturing process.

The invention discloses a method for agrobacterium mediated pepper transgene. The invention comprises the following steps: firstly, a young and tender stem section with an axillary bud and a stem tip are inoculated in an induction culture medium, and a leaf stalk and the stem section of the sprouting axillary bud for 20 days are used as a dip-dyeing explant; secondly, the single colony of agrobacterium is picked with toothpicks and added into a YEP liquid culture medium to cultivate shaking culture till an OD value reaches 0.4 to 0.7, thallus is centrifugally collected, and agrobacterium liquid is prepared by the weight dropping of a WPM weigh dropping culture medium; thirdly, the leaf stalk and the stem section are cut into about 0.5 centimeters explants, inoculated with the induction culture medium to perform pre-incubation in dark, the cultivation time needs 48 to 72 hours, the explants are thrown into the agrobacterium liquid of the step b, to ensure that the explants are adequately contacted with the agrobacterium, the explants are taken out, shifted on the common culture medium to be cultivated for 48 to 72 hours, and then shifted in the sieving medium to induce buds to be regenerated, finally material is inoculated in the rooting culture medium to induce root regenerating. The invention can cultivate transgene pepper with insect resistance, low temperature resistance, drought resistance, high essential oil content, high medicinal value, and no thorn.

Capsules which contain vital cells or tissues as the contents and in which these cells or tissues can be grown. Since these cells or tissues can be grown in the capsules, an extremely high cell density can be achieved. By using these capsules, foods having an excellent effect of ameliorating intestinal disorders, etc. can be provided. Moreover, it is possible to provide artificial seeds showing an excellent storage stability and a high germination ratio which comprise seamless soft capsules consisting of an innermost layer containing indefinite embryo, indefinite shoots, multiple shoots, shoot apexes, growing points, protocorm-like bodies, indefinite roots, hairy roots, etc., an inner coating layer comprising a hardened oil and an outer coating layer made of a biodegradable film comprising gelatin, polysaccharides, etc.

The invention provides a robina stem-apex chromosome pressing method, comprising: taking material, preprocessing, fixing, dissociating, pressing, dyeing, striking, microscopic examining, characterized in that: taking the stem-apex of robina tissue culture seedling in vigorous growth stage at 3-5pm at the culture-room temperature 22-25 DEG C., wherein the cell of the stem-apex of robina tissue culture seedling is at vigorous division stage; preprocessing the stem-apex using 8-hydroxy quinoline 0.002-0.004m for 3-5 hours; pressing the stem-apex using a cross pressing method; dyeing the pressed stem-apex using carbol fuchsin; striking the material using a pencil head and uniformly dispersing the cells to the best. The robina stem-apex chromosome pressing method has features of simple operation, high pressing efficiency, saved medicine consumption, uniform chromosome dispersion, easy counting, very easy observation of metaphase chromosome, uniform and clear dyeing, especially suitable for chromosome pressing of tough wood material.